FastQCFastQC Report
Fri 27 May 2016
SRR2139261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23537327
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298650.048.861451
GTATCAA526900.034.9999121
ATCAACG654700.027.5383663
TCAACGC659450.027.3527494
CAACGCA668800.026.9452825
AACGCAG681850.026.5158256
TATCAAC693400.026.2498992
ACGCAGA784300.022.9396937
CGCAGAG789850.022.7785028
GTACATG699850.019.4864461
TACATGG687750.019.260462
GCAGAGT936450.019.042149
ACATGGG701300.018.4547713
GAGTACT542950.018.3327612-13
AGAGTAC850600.018.30744410-11
CAGAGTA910600.017.5993810-11
CATGGGG478700.016.4359634
AGTACTT610000.015.95611512-13
GTACTTT635100.015.65390114-15
GTGGTAT141700.015.3774561
Produced by FastQC (version 0.11.5)