FastQCFastQC Report
Fri 27 May 2016
SRR2139260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786312
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120350.050.3840981
GTATCAA226750.040.972411
ATCAACG272100.033.169813
TCAACGC274250.032.9732744
CAACGCA278950.032.3547825
TATCAAC291050.031.4146442
AACGCAG289350.031.235816
ACGCAGA329400.027.4248687
CGCAGAG332500.027.1301028
GCAGAGT402950.022.3545559
TACATGG333100.021.8186992
GTACATG343100.021.7003481
ACATGGG336700.021.1333473
GAGTACT214950.019.80457112-13
AGAGTAC367350.019.51055310-11
CAGAGTA393950.019.01214210-11
CATGGGG214900.019.0043724
AGTACTT239950.017.09143812-13
GTACTTT248100.017.08881614-15
GTGGTAT59550.015.4193331
Produced by FastQC (version 0.11.5)