##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9786312
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.797308015522088	33.0	31.0	33.0	25.0	34.0
2	31.838887008711758	33.0	32.0	33.0	28.0	34.0
3	31.89109993631922	33.0	31.0	33.0	29.0	34.0
4	32.30801409151885	33.0	33.0	33.0	31.0	34.0
5	32.79958466478485	33.0	33.0	34.0	32.0	34.0
6	36.46494368869499	38.0	37.0	38.0	34.0	38.0
7	37.10950110726083	38.0	38.0	38.0	36.0	38.0
8	37.05611552135268	38.0	38.0	38.0	36.0	38.0
9	37.23339670756461	38.0	38.0	38.0	36.0	38.0
10-11	37.31596407308494	38.0	38.0	38.0	37.0	38.0
12-13	37.359558432226564	38.0	38.0	38.0	37.0	38.0
14-15	37.394666857136784	38.0	38.0	38.0	37.0	38.0
16-17	37.33100457046536	38.0	38.0	38.0	37.0	38.0
18-19	37.37836051006753	38.0	38.0	38.0	37.0	38.0
20-21	37.41173737535516	38.0	38.0	38.0	37.0	38.0
22-23	37.426920822039634	38.0	38.0	38.0	37.0	38.0
24-25	37.429431580803225	38.0	38.0	38.0	37.0	38.0
26-27	37.39847732644232	38.0	38.0	38.0	37.0	38.0
28-29	37.375547482041235	38.0	38.0	38.0	37.0	38.0
30-31	37.27647944861721	38.0	38.0	38.0	37.0	38.0
32-33	37.338592277025725	38.0	38.0	38.0	37.0	38.0
34-35	37.35183576462092	38.0	38.0	38.0	37.0	38.0
36-37	37.33662460317541	38.0	38.0	38.0	37.0	38.0
38-39	37.31404200211021	38.0	38.0	38.0	37.0	38.0
40-41	37.2952926307988	38.0	38.0	38.0	37.0	38.0
42-43	37.27410183083947	38.0	38.0	38.0	37.0	38.0
44-45	37.23911348015801	38.0	38.0	38.0	37.0	38.0
46-47	37.20999991729802	38.0	38.0	38.0	37.0	38.0
48-49	37.18058728968048	38.0	38.0	38.0	37.0	38.0
50-51	37.141460491309005	38.0	38.0	38.0	37.0	38.0
52-53	37.121686093975114	38.0	38.0	38.0	36.0	38.0
54-55	37.10507300500173	38.0	38.0	38.0	36.0	38.0
56-57	37.09281884265016	38.0	38.0	38.0	36.0	38.0
58-59	37.08129158906433	38.0	38.0	38.0	36.0	38.0
60-61	37.08019501984681	38.0	38.0	38.0	36.0	38.0
62-63	37.08382005572243	38.0	38.0	38.0	36.0	38.0
64-65	37.08421566738623	38.0	38.0	38.0	36.0	38.0
66-67	37.06387782059116	38.0	38.0	38.0	36.0	38.0
68-69	37.063792696708084	38.0	38.0	38.0	36.0	38.0
70-71	37.04370927527023	38.0	38.0	38.0	36.0	38.0
72-73	37.03196091776631	38.0	38.0	38.0	36.0	38.0
74-75	37.016020550249635	38.0	38.0	38.0	36.0	38.0
76-77	37.0150108800576	38.0	38.0	38.0	36.0	38.0
78-79	36.99518404132276	38.0	38.0	38.0	36.0	38.0
80-81	36.987242815346264	38.0	38.0	38.0	36.0	38.0
82-83	36.93099589779226	38.0	38.0	38.0	36.0	38.0
84-85	36.9149413794211	38.0	38.0	38.0	36.0	38.0
86-87	36.90307846725277	38.0	38.0	38.0	35.0	38.0
88-89	36.90588650310232	38.0	38.0	38.0	35.0	38.0
90-91	36.89413742136696	38.0	38.0	38.0	35.0	38.0
92-93	36.873861267854004	38.0	38.0	38.0	35.0	38.0
94-95	36.84382595309608	38.0	38.0	38.0	35.0	38.0
96-97	36.81129896099212	38.0	38.0	38.0	35.0	38.0
98-99	36.8143190034168	38.0	38.0	38.0	35.0	38.0
100	35.2151868960609	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	12.0
19	24.0
20	62.0
21	221.0
22	823.0
23	2154.0
24	4956.0
25	9830.0
26	16442.0
27	26266.0
28	39812.0
29	57462.0
30	78901.0
31	104076.0
32	135240.0
33	181383.0
34	263323.0
35	455394.0
36	1206203.0
37	7203725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29736513612074	19.818436199459	14.132545539116267	24.751653125303996
2	13.576812018371577	21.415020838885738	43.08962846449541	21.918538678247277
3	17.04137370645857	27.776418736700816	30.927861282166358	24.25434627467426
4	11.390878578439413	18.863605823297174	41.39225300982591	28.353262588437506
5	12.481338170438681	38.35971893777428	36.3005667882483	12.858376103538744
6	27.507543188894857	39.61328843797337	20.27128299199944	12.607885381132341
7	24.957879944968035	33.547663307689355	24.72286802219263	16.771588725149986
8	23.6604861974562	36.77628508063099	22.40169739121336	17.16153133069945
9	25.758894239464684	16.786831520625103	21.86128372809356	35.592990511816645
10-11	23.2502325584247	27.16922073474412	30.061150590821274	19.519396116009908
12-13	24.558149411042148	24.915480158178084	29.865245961157072	20.661124469622695
14-15	21.817161561985763	25.90971961654196	27.6412605688435	24.631858252628774
16-17	21.10324604406645	29.051832804840068	28.132196275777844	21.712724875315644
18-19	21.165506474757805	28.29849998651177	29.85187371912933	20.68411981960109
20-21	22.249924600176566	27.44896914914614	29.502199712890555	20.798906537786742
22-23	21.874405372013577	27.421769694640965	29.394299542283353	21.30952539106211
24-25	21.487063921550593	27.58005140705084	29.56339777099845	21.369486900400116
26-27	21.505023772880136	27.736040411979783	29.37957471752217	21.379361097617906
28-29	21.40853151905932	27.723242441219337	29.46556324436747	21.402662795353873
30-31	21.751222177097574	27.536447875555016	29.353927568695116	21.358402378652293
32-33	21.4990692934901	27.76073130790526	29.345028168181226	21.39517123042342
34-35	21.59147675165683	27.77438697061968	29.41596779726452	21.218168480458964
36-37	21.60204402531107	27.6601266707001	29.3504682885799	21.387361015408928
38-39	21.45516833392957	27.78470412065236	29.427160489995003	21.332967055423072
40-41	21.67578324029495	27.62477082855961	29.326812713907437	21.37263321723801
42-43	21.59006917880508	27.681537032042613	29.223072948053424	21.50532084109888
44-45	21.638731026544853	27.813637948820553	29.083017662731237	21.46461336190336
46-47	21.69114378638177	27.775982734219657	28.858126289416308	21.67474718998227
48-49	21.676445914921715	27.863845392531054	28.857715302431806	21.601993390115425
50-51	21.643425992614315	28.01825526775103	28.654996872240883	21.683321867393772
52-53	21.745722307059424	27.971540478499858	28.593892144015133	21.688845070425586
54-55	21.765009491735775	27.89887004806189	28.6345218010224	21.701598659179936
56-57	21.74370805728048	28.002279801476703	28.561260093293928	21.69275204794889
58-59	21.711714737002684	27.992454498387296	28.634498168579903	21.661332596030118
60-61	21.77703747255434	27.993809570392596	28.57087927271133	21.658273684341733
62-63	21.724462171415272	28.022148697450532	28.569587395522777	21.683801735611414
64-65	21.676991944927835	27.97086261432432	28.596929067258102	21.755216373489745
66-67	21.807425096154912	28.000998879105193	28.39698390843305	21.794592116306845
68-69	21.729023795553548	28.029289040485818	28.46530700757742	21.776380156383215
70-71	21.802082730380228	27.754981291371017	28.544238114092018	21.898697864156738
72-73	21.835870363317937	27.79497967246157	28.482383480426837	21.886766483793664
74-75	21.6907293202724	27.930942877914923	28.463138443960805	21.91518935785187
76-77	21.811655421661776	27.906492491238666	28.361462872236316	21.920389214863246
78-79	21.793463645816765	27.934992307446134	28.31935729321455	21.952186753522547
80-81	21.822448994095982	27.924215428836508	28.26332324576466	21.99001233130285
82-83	21.851428692267007	27.852748053528924	28.38144051498841	21.914382739215657
84-85	22.000084189458637	27.86933943075794	28.276071327711268	21.85450505207216
86-87	21.790587578341103	27.922473657746522	28.355729447853516	21.93120931605886
88-89	21.901748438099137	27.89223982050561	28.264965419669064	21.94104632172619
90-91	21.98466311746834	27.9059952010627	28.28243840940453	21.826903272064435
92-93	21.978183669395214	27.902762477231676	28.240350975749017	21.87870287762409
94-95	21.801493626652316	27.99884864008431	28.255096738914077	21.944560994349295
96-97	21.901398717148066	28.137394219845884	28.33247711918852	21.62872994381753
98-99	22.967684748048946	30.03009223169161	28.57626150706409	18.42596151319535
100	21.88210641598762	30.92670843008137	22.585566755478233	24.60561839845278
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1833.0
1	1933.0
2	2913.5
3	4192.5
4	5455.5
5	7442.0
6	9021.5
7	10919.0
8	13305.5
9	14416.0
10	14114.0
11	13967.5
12	15446.0
13	18674.0
14	24305.5
15	30418.0
16	34644.0
17	36764.5
18	35842.0
19	32532.0
20	29524.5
21	28619.0
22	31112.0
23	37934.0
24	49608.5
25	66907.5
26	88255.5
27	111123.0
28	133873.5
29	157578.5
30	180231.0
31	202907.0
32	228230.5
33	252453.5
34	276659.5
35	300176.0
36	324507.5
37	347293.5
38	361369.5
39	371106.0
40	383040.5
41	391109.0
42	395252.0
43	401865.5
44	406218.0
45	408186.5
46	407181.5
47	402937.0
48	396632.0
49	385740.5
50	371622.5
51	354555.5
52	333550.5
53	310935.0
54	287438.0
55	262174.5
56	237694.0
57	211388.5
58	184678.0
59	160433.0
60	134268.0
61	107385.0
62	82495.0
63	62155.0
64	46692.5
65	34280.0
66	24615.5
67	18130.5
68	13690.0
69	9624.5
70	6668.0
71	4797.0
72	3333.0
73	2244.0
74	1420.5
75	868.5
76	567.0
77	343.5
78	200.5
79	136.5
80	86.5
81	54.5
82	36.0
83	24.5
84	17.5
85	13.5
86	11.0
87	10.0
88	6.5
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007765949011231198
3	0.0
4	0.004567604221079402
5	0.003004196064871016
6	0.0
7	0.0
8	0.0
9	3.474240347129746E-4
10-11	6.284287686719982E-4
12-13	5.109176981073156E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.605928043014937E-4
24-25	0.0014921108217279134
26-27	0.0
28-29	0.0019272353629797003
30-31	0.002941713201585169
32-33	5.841541724287838E-4
34-35	3.594191540012492E-5
36-37	0.0011380284301129532
38-39	0.002322810192677364
40-41	0.0014668837724006027
42-43	0.0
44-45	0.0
46-47	0.001282872752858858
48-49	5.393920410835158E-4
50-51	0.001613884745389239
52-53	0.002409764865433315
54-55	0.0018472007525245212
56-57	0.0019197266835756191
58-59	0.0019496326488907668
60-61	8.958814881225868E-4
62-63	7.680183265990646E-4
64-65	0.003314293180116251
66-67	0.002434566336898589
68-69	1.0894416703182075E-4
70-71	0.002798684225210582
72-73	0.009767695054309841
74-75	0.012094024517608265
76-77	0.004737631450766386
78-79	0.004310605773847471
80-81	3.1319026079473474E-4
82-83	8.507296404293949E-5
84-85	6.127322201500299E-5
86-87	0.0020569328117412936
88-89	0.004391850221087609
90-91	5.469883419180115E-4
92-93	0.0010156179829128623
94-95	0.0022180495254559966
96-97	0.0024509098043738404
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	392.0
22-23	881.0
24-25	873.0
26-27	2127.0
28-29	5237.0
30-31	14851.0
32-33	18497.0
34-35	24612.0
36-37	40601.0
38-39	53258.0
40-41	51117.0
42-43	56149.0
44-45	67697.0
46-47	70417.0
48-49	68766.0
50-51	63417.0
52-53	56778.0
54-55	59457.0
56-57	62040.0
58-59	65996.0
60-61	68061.0
62-63	63744.0
64-65	64110.0
66-67	69067.0
68-69	72382.0
70-71	74418.0
72-73	66992.0
74-75	64739.0
76-77	67025.0
78-79	72041.0
80-81	74427.0
82-83	69508.0
84-85	65487.0
86-87	67227.0
88-89	72135.0
90-91	75861.0
92-93	76019.0
94-95	87579.0
96-97	294408.0
98-99	673700.0
100-101	6764134.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.78552547339079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13737122785365	72.96498590941177
2	8.0594950514954	13.344190657764788
3	2.053503015113761	5.100009785021551
4	0.8424559267334409	2.789726263312013
5	0.3574850138749755	1.4797292361251122
6	0.19034762325939739	0.945481680848414
7	0.11081563402424459	0.6421751345354836
8	0.06962052709750456	0.46108575356010856
9	0.039286154062996355	0.2927092417140365
>10	0.1359792265064755	1.7092706000132816
>50	0.0030437416757386274	0.1662826911598503
>100	5.755218326470853E-4	0.08255627677745107
>500	0.0	0.0
>1k	2.1336469890619518E-5	0.021796769756063733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0436707924292624E-5	0.0
3	0.0	0.0	0.0	2.0436707924292624E-5	0.0
4	0.0	2.0436707924292624E-5	0.0	2.0436707924292624E-5	0.0
5	0.0	3.0655061886438937E-5	0.0	2.0436707924292624E-5	0.0
6	0.0	3.0655061886438937E-5	0.0	2.0436707924292624E-5	0.0
7	0.0	5.109176981073156E-5	0.0	2.0436707924292624E-5	0.0
8	0.0	5.109176981073156E-5	0.0	2.0436707924292624E-5	0.0
9	0.0	8.17468316971705E-5	0.0	2.0436707924292624E-5	2.0436707924292624E-5
10-11	0.0	9.196518565931681E-5	0.0	3.0655061886438937E-5	2.0436707924292624E-5
12-13	0.0	9.196518565931681E-5	0.0	3.0655061886438937E-5	2.554588490536578E-5
14-15	0.0	2.0436707924292624E-4	0.0	4.087341584858525E-5	4.5982592829658405E-5
16-17	0.0	2.1458543320507256E-4	0.0	4.087341584858525E-5	5.109176981073156E-5
18-19	0.0	2.4013131811043834E-4	0.0	4.087341584858525E-5	5.109176981073156E-5
20-21	0.0	2.656772030158041E-4	0.0	4.5982592829658405E-5	5.620094679180472E-5
22-23	0.0	2.7589555697795043E-4	0.0	6.131012377287787E-5	7.152847773502419E-5
24-25	0.0	3.0144144188331624E-4	0.0	7.663765471609734E-5	9.196518565931681E-5
26-27	0.0	3.167689728265357E-4	0.0	1.1240189358360943E-4	1.0218353962146312E-4
28-29	0.0	3.372056807508283E-4	0.0	2.7589555697795043E-4	1.0218353962146312E-4
30-31	0.0	4.087341584858525E-4	0.0	9.247610335742412E-4	1.0218353962146312E-4
32-33	5.109176981073156E-6	4.24061689429072E-4	0.0	0.0024677324818583343	1.2262024754575575E-4
34-35	2.0436707924292624E-5	4.90480990183023E-4	0.0	0.004230398540328573	1.5327530943219468E-4
36-37	3.0655061886438937E-5	5.56900290936974E-4	0.0	0.007050664233880955	1.5327530943219468E-4
38-39	3.0655061886438937E-5	5.92664529804486E-4	0.0	0.015296875881333029	1.5327530943219468E-4
40-41	5.109176981073156E-5	6.744113615016566E-4	0.0	0.025193351693671734	1.5327530943219468E-4
42-43	5.109176981073156E-5	7.050664233880955E-4	0.0	0.03121196217737591	1.5327530943219468E-4
44-45	5.109176981073156E-5	7.714857241420466E-4	0.0	0.03773127200522526	1.5838448641326784E-4
46-47	5.620094679180472E-5	8.123591399906318E-4	0.0	0.044807482124011574	1.63493663394341E-4
48-49	7.152847773502419E-5	8.327958479149244E-4	0.0	0.05219535203864337	1.737120173564873E-4
50-51	7.152847773502419E-5	8.787784407445827E-4	0.0	0.06009413965138247	1.737120173564873E-4
52-53	7.152847773502419E-5	8.889967947067291E-4	0.0	0.077940494846271	1.7882119433756046E-4
54-55	8.17468316971705E-5	8.992151486688755E-4	0.0	0.09890344799961415	1.8903954829970678E-4
56-57	8.685600867824365E-5	9.298702105553144E-4	0.0	0.12440335031215027	1.992579022618531E-4
58-59	9.196518565931681E-5	9.451977414985338E-4	0.0	0.14649032240132953	2.0436707924292624E-4
60-61	1.0218353962146312E-4	9.605252724417533E-4	0.0	0.155671513436318	2.0436707924292624E-4
62-63	1.0218353962146312E-4	9.656344494228265E-4	0.0	0.16664602559166314	2.1458543320507256E-4
64-65	1.0729271660253628E-4	0.0011086914048928748	0.0	0.17196978800594137	2.1458543320507256E-4
66-67	1.1240189358360943E-4	0.0011240189358360942	0.0	0.17640966280249393	2.1458543320507256E-4
68-69	1.1240189358360943E-4	0.0011648923516846795	0.0	0.18108455974017587	2.1458543320507256E-4
70-71	1.1240189358360943E-4	0.0011904382365900453	0.0	0.18553976206767164	2.2991296414829202E-4
72-73	1.1240189358360943E-4	0.0011955474135711185	0.0	0.18909574924649858	2.452404950915115E-4
74-75	1.2262024754575575E-4	0.0012415300064007768	0.0	0.1918342681083538	2.452404950915115E-4
76-77	1.2262024754575575E-4	0.0013539318999843863	0.0	0.19463920627096293	2.452404950915115E-4
78-79	1.2262024754575575E-4	0.001410132846776191	0.0	0.19643252739131964	2.452404950915115E-4
80-81	1.277294245268289E-4	0.001410132846776191	0.0	0.19707628369093483	2.503496720725846E-4
82-83	1.3283860150790206E-4	0.0014203512007383372	0.0	0.1974441444335721	2.554588490536578E-4
84-85	1.3283860150790206E-4	0.0014458970856437033	0.0	0.19759741974300432	2.554588490536578E-4
86-87	1.3283860150790206E-4	0.001471442970549069	0.0	0.19768427575168257	2.60568026034731E-4
88	1.3283860150790206E-4	0.001604281572056971	0.0	0.1976944941056447	2.656772030158041E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12035	0.0	50.384098	1
GTATCAA	22675	0.0	40.97241	1
ATCAACG	27210	0.0	33.16981	3
TCAACGC	27425	0.0	32.973274	4
CAACGCA	27895	0.0	32.354782	5
TATCAAC	29105	0.0	31.414644	2
AACGCAG	28935	0.0	31.23581	6
ACGCAGA	32940	0.0	27.424868	7
CGCAGAG	33250	0.0	27.130102	8
GCAGAGT	40295	0.0	22.354555	9
TACATGG	33310	0.0	21.818699	2
GTACATG	34310	0.0	21.700348	1
ACATGGG	33670	0.0	21.133347	3
GAGTACT	21495	0.0	19.804571	12-13
AGAGTAC	36735	0.0	19.510553	10-11
CAGAGTA	39395	0.0	19.012142	10-11
CATGGGG	21490	0.0	19.004372	4
AGTACTT	23995	0.0	17.091438	12-13
GTACTTT	24810	0.0	17.088816	14-15
GTGGTAT	5955	0.0	15.419333	1
>>END_MODULE