FastQCFastQC Report
Fri 27 May 2016
SRR2139259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9989190
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127250.052.9363631
GTATCAA247450.040.094151
ATCAACG292300.033.247753
TCAACGC295600.032.9205864
CAACGCA301150.032.2830355
TATCAAC307300.031.90722
AACGCAG311350.031.265336
ACGCAGA354700.027.4442067
CGCAGAG359300.027.080878
GTACATG375700.022.8444921
GCAGAGT428400.022.7831049
TACATGG370800.022.6472282
ACATGGG370200.022.1126693
CTTATAC63200.020.0910421
GAGTACT225150.019.84939612-13
AGAGTAC389300.019.56607810-11
CAGAGTA420300.019.4386510-11
CATGGGG221300.018.3984474
AGTACTT246250.017.22237812-13
GTACTTT261650.016.94095614-15
Produced by FastQC (version 0.11.5)