FastQCFastQC Report
Fri 27 May 2016
SRR2139258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23368182
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271700.045.9561421
GTATCAA478550.035.5303461
ATCAACG592250.028.0314713
TCAACGC595500.027.8925934
CAACGCA604950.027.408345
AACGCAG620050.026.7882826
TATCAAC630850.026.5192782
ACGCAGA708300.023.3794677
CGCAGAG713500.023.2149648
GTACATG640100.020.2623371
TACATGG636550.019.694842
ACATGGG640550.019.2039453
GCAGAGT853600.019.178579
AGAGTAC774900.018.34633310-11
GAGTACT510900.017.5754412-13
CAGAGTA839150.016.99919110-11
CATGGGG439350.016.747414
AGTACTT567200.016.13079312-13
GTACTTT591900.015.16784114-15
GTGGTAT141350.013.9540421
Produced by FastQC (version 0.11.5)