##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23368182
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6795784969494	31.0	31.0	34.0	30.0	34.0
2	31.816149283671276	31.0	31.0	34.0	30.0	34.0
3	31.861164895069717	31.0	31.0	34.0	30.0	34.0
4	35.61016368325101	37.0	35.0	37.0	33.0	37.0
5	35.463106543761086	37.0	35.0	37.0	33.0	37.0
6	35.48283580639692	37.0	35.0	37.0	33.0	37.0
7	35.39160449024233	37.0	35.0	37.0	33.0	37.0
8	35.3773444164377	37.0	35.0	37.0	33.0	37.0
9	36.98490344691769	39.0	37.0	39.0	33.0	39.0
10-11	36.87354737309047	39.0	37.0	39.0	32.5	39.0
12-13	36.80662434501751	39.0	37.0	39.0	32.0	39.0
14-15	37.96810947894877	40.0	37.0	41.0	33.0	41.0
16-17	37.94728430307501	40.0	37.0	41.0	33.0	41.0
18-19	37.968114528549975	40.0	37.0	41.0	33.0	41.0
20-21	37.94684324685169	40.0	37.0	41.0	32.0	41.0
22-23	37.850730558410476	40.0	37.0	41.0	32.0	41.0
24-25	37.74833393597663	40.0	37.0	41.0	32.0	41.0
26-27	37.563444770902805	39.0	36.5	41.0	32.0	41.0
28-29	37.437463453550016	39.0	36.0	41.0	31.5	41.0
30-31	37.2632950338407	39.0	36.0	41.0	31.0	41.0
32-33	37.07178722918799	39.0	36.0	40.5	31.0	41.0
34-35	36.95158215693598	39.0	35.5	40.0	30.0	41.0
36-37	36.8017908205791	39.0	35.0	40.0	30.0	41.0
38-39	36.657318650349154	38.0	35.0	40.0	30.0	41.0
40-41	36.535072696531316	38.0	35.0	40.0	30.0	41.0
42-43	36.35167631951484	38.0	35.0	40.0	30.0	41.0
44-45	36.19474764158285	38.0	35.0	40.0	30.0	41.0
46-47	36.32784074337954	38.0	35.0	40.0	30.0	41.0
48-49	36.74141404609672	39.0	35.0	40.0	30.0	41.0
50-51	36.84092671435215	39.0	35.0	41.0	30.0	41.0
52-53	36.80515629289775	39.0	35.0	41.0	30.0	41.0
54-55	36.70587326832944	39.0	35.0	40.5	30.0	41.0
56-57	36.57478640782446	39.0	35.0	40.0	30.0	41.0
58-59	36.38518524937972	38.0	35.0	40.0	29.5	41.0
60-61	36.22215448453943	38.0	34.0	40.0	29.0	41.0
62-63	36.02428683773729	38.0	34.0	40.0	29.0	41.0
64-65	35.78791152615197	37.5	34.0	40.0	28.0	41.0
66-67	35.53836105693311	37.0	34.0	40.0	28.0	41.0
68-69	35.271818607948404	36.5	34.0	39.5	28.0	41.0
70-71	34.99034735478237	36.0	33.0	39.0	28.0	41.0
72-73	34.68244861210556	36.0	33.0	39.0	28.0	40.0
74-75	34.3649812131551	35.0	33.0	38.5	28.0	40.0
76-77	32.577450864272166	34.0	30.5	36.0	26.0	39.0
78-79	33.626395547324066	35.0	32.0	37.0	27.0	39.0
80-81	33.651188799146546	35.0	32.0	36.5	28.0	39.0
82-83	33.41331982841981	35.0	32.0	36.0	27.0	38.5
84-85	33.19082985349524	35.0	32.0	36.0	27.0	37.0
86-87	32.97331512295325	34.5	32.0	35.5	27.0	37.0
88-89	32.81482909025703	34.0	32.0	35.0	27.0	36.5
90-91	32.68171559960139	34.0	32.0	35.0	27.0	36.0
92-93	32.5968788997109	34.0	32.0	35.0	27.0	36.0
94-95	32.56689363548888	34.0	32.0	35.0	27.0	36.0
96-97	32.64301170677085	34.0	32.0	35.0	27.5	35.0
98-99	32.784457830617804	34.0	32.0	35.0	29.0	35.0
100	31.416852311353118	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	25.0
20	257.0
21	1721.0
22	8249.0
23	27149.0
24	68585.0
25	137935.0
26	237576.0
27	358616.0
28	493335.0
29	630235.0
30	771444.0
31	923022.0
32	1101123.0
33	1332892.0
34	1655456.0
35	2114288.0
36	2884029.0
37	4078814.0
38	4449093.0
39	2029249.0
40	65083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65652954945318	21.870837021039975	14.920510290445357	23.552123139061482
2	14.167814026231234	22.783233398883066	41.433679717432646	21.615272857453053
3	17.288644876182495	28.265125631082473	31.53041601610258	22.915813476632458
4	12.089725443180482	19.821892481607005	40.34307063996386	27.745311435248652
5	12.14986685742177	38.999157058944505	36.64789584401559	12.203080239618126
6	27.558275607404976	40.38873456223509	19.774315348964674	12.278674481395257
7	24.45626811945697	33.657476377814774	25.073710272956205	16.812545229772056
8	23.383333799779546	37.66508665500808	22.237459465182184	16.714120080030188
9	25.15468580665799	16.91127355901777	22.03627369274665	35.89776694157759
10-11	23.07528606933851	27.530858288359912	30.265280469623733	19.12857517267784
12-13	24.282646316486776	25.13915931835513	30.056748107897146	20.52144625726094
14-15	21.39523447940244	26.387833794674346	27.61354278232538	24.603388943597828
16-17	20.900998154855458	29.45976297229647	28.2096226618002	21.42961621104787
18-19	20.951258465517704	28.78313677902444	29.742193027347742	20.523411728110116
20-21	21.804735169329906	28.00279689580258	29.504657274022883	20.68781066084463
22-23	21.483068682548517	27.991579440104207	29.363531967863395	21.16181990948388
24-25	21.291687392550543	28.103630596831596	29.450863893374812	21.15381811724305
26-27	21.318464606118237	28.25136001457331	29.281812739259443	21.148362640049005
28-29	21.24809411232245	28.269221068693117	29.304889055969184	21.177795763015244
30-31	21.521528203178963	28.174115229705478	29.20789700518806	21.096459561927503
32-33	21.323502886274657	28.288862338470082	29.18340429309194	21.204230482163318
34-35	21.38187222968544	28.32765944098763	29.196297878883996	21.094170450442927
36-37	21.41142851555487	28.297802598462297	29.147848820168694	21.142920065814142
38-39	21.29675887036875	28.339160022226523	29.183568185648472	21.180512921756257
40-41	21.519268743949524	28.21236247413189	29.069267067584644	21.199101714333942
42-43	21.466014867695353	28.348933852246493	28.860629190947435	21.32442208911072
44-45	21.502381511541	28.493649302557333	28.680712256792486	21.32325692910918
46-47	21.577283424816162	28.45972154991368	28.40971622933494	21.553278795935213
48-49	21.560271726177767	28.513332998804348	28.44240799671202	21.483987278305865
50-51	21.532415269335907	28.636514725557	28.294761889767965	21.536308115339125
52-53	21.62651128559954	28.55999360178898	28.260982823748737	21.55251228886274
54-55	21.60034006228935	28.50596325248691	28.298851890584505	21.594844794639233
56-57	21.640077660866204	28.6023269761757	28.232602937918998	21.524992425039095
58-59	21.649256348681345	28.64920318861496	28.255516759590588	21.446023703113106
60-61	21.676656862057325	28.668149050229186	28.190237367196136	21.46495672051735
62-63	21.578845563659467	28.699636014927155	28.203012149468044	21.518506271945334
64-65	21.556551960624766	28.648410696706083	28.211746652261095	21.583290690408056
66-67	21.632321388136123	28.665355057274994	28.083850055355697	21.618473499233186
68-69	21.594632505855902	28.650898529828446	28.16088134051869	21.593587623796964
70-71	21.637432015876936	28.42255295267744	28.20880143750555	21.731213593940076
72-73	21.656748500487215	28.489574367784666	28.19735821047112	21.656318921257
74-75	21.506557067158962	28.585944993197653	28.185350120983728	21.722147818659653
76-77	21.55507499296578	28.58965125656372	28.17102638567921	21.684247364791297
78-79	21.584638603181972	28.59311865378899	28.147678075587514	21.674564667441523
80-81	21.565780620495072	28.600308675579384	28.15800474179644	21.675905962129107
82-83	21.59404427136141	28.553022017324047	28.248628243644237	21.604305467670308
84-85	21.665532941448063	28.536136088399317	28.20281867343366	21.59551229671896
86-87	21.503822769388385	28.58975978984453	28.2742845088842	21.632132931882893
88-89	21.58000662463515	28.54384857329706	28.234446970361837	21.641697831705955
90-91	21.576279084944336	28.57260936868157	28.324316506310083	21.52679504006402
92-93	21.5804065498474	28.56522278599784	28.26999361989711	21.58437704425765
94-95	21.363589381488215	28.623334284812884	28.400087135495696	21.612989198203206
96-97	21.383157767473822	28.761628974680097	28.525179475668622	21.330033782177455
98-99	22.365334595606363	30.773965160731144	28.805681923944178	18.055018319718315
100	21.751247233974603	31.473267165150798	22.644367076649687	24.131118524224913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5502.0
1	4947.0
2	7312.5
3	10799.0
4	14266.0
5	22577.0
6	29736.0
7	34520.0
8	37190.0
9	35764.0
10	31972.0
11	28300.0
12	30135.0
13	38892.0
14	60658.5
15	91699.5
16	116740.5
17	131399.5
18	136412.5
19	133467.5
20	126429.0
21	119607.0
22	119241.5
23	132560.0
24	159942.0
25	200780.0
26	253798.5
27	311281.5
28	369594.5
29	430303.0
30	487302.0
31	541318.0
32	600537.5
33	658556.5
34	714015.0
35	767232.5
36	821399.5
37	870431.5
38	903660.0
39	925773.5
40	947827.0
41	964516.0
42	971627.0
43	978793.0
44	982664.5
45	982677.0
46	973144.5
47	958583.0
48	944269.0
49	914997.0
50	878894.0
51	838476.5
52	786946.0
53	731422.0
54	670648.0
55	605641.0
56	545802.5
57	487102.0
58	425545.0
59	366214.0
60	306567.5
61	248846.5
62	197222.0
63	153099.0
64	115408.5
65	86216.0
66	65227.0
67	50554.5
68	39226.0
69	28568.5
70	20450.5
71	15158.5
72	11089.0
73	7903.0
74	5631.0
75	3960.0
76	2785.5
77	1914.0
78	1317.0
79	909.5
80	635.5
81	462.5
82	355.0
83	252.5
84	173.0
85	137.5
86	119.5
87	99.0
88	73.5
89	50.5
90	35.5
91	26.0
92	19.5
93	10.5
94	3.5
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1400151710560967
3	0.0
4	2.1396615277987822E-4
5	0.0
6	0.0
7	4.279323055597564E-6
8	0.0
9	2.995526138918295E-5
10-11	1.17681384028933E-4
12-13	8.558646111195128E-6
14-15	3.1666990611421973E-4
16-17	0.001561952915293111
18-19	4.279323055597564E-6
20-21	5.691847850915527E-4
22-23	0.002285654264359928
24-25	0.0
26-27	0.004849538775446905
28-29	0.003805038488339454
30-31	0.009076627863581632
32-33	0.00972371687721735
34-35	0.003119422682723368
36-37	0.00795610432971848
38-39	2.9655047041517656E-4
40-41	1.5369420263711145E-5
42-43	0.002097594013201168
44-45	3.125342252881532E-5
46-47	0.0
48-49	1.5709480792325197E-4
50-51	0.0
52-53	4.631015289435909E-6
54-55	0.002495452219244876
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	4.8958580013178665E-6
66-67	0.0
68-69	1.2748544753616374E-4
70-71	4.980288674722431E-4
72-73	4.6281833815128924E-4
74-75	4.4736755612387617E-4
76-77	0.0010905248039474384
78-79	0.006794645628814566
80-81	8.088720731586757E-4
82-83	4.0175951120852035E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.054217141153494E-4
94-95	9.014008670875408E-5
96-97	4.962537032544907E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	844.0
20-21	2977.0
22-23	6002.0
24-25	12215.0
26-27	16284.0
28-29	25857.0
30-31	57037.0
32-33	65451.0
34-35	89590.0
36-37	122996.0
38-39	156321.0
40-41	169284.0
42-43	192083.0
44-45	227874.0
46-47	213203.0
48-49	193828.0
50-51	179890.0
52-53	172692.0
54-55	185273.0
56-57	198137.0
58-59	202049.0
60-61	206263.0
62-63	197538.0
64-65	200840.0
66-67	216440.0
68-69	222849.0
70-71	225279.0
72-73	219422.0
74-75	221192.0
76-77	206690.0
78-79	232157.0
80-81	249576.0
82-83	243329.0
84-85	244051.0
86-87	260754.0
88-89	290758.0
90-91	326506.0
92-93	368567.0
94-95	454755.0
96-97	958110.0
98-99	1656555.0
100-101	1.3676664E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.73667272702285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.87406358146772	71.82533576152025
2	7.956650000009027	13.007001941084084
3	2.1869829935913154	5.362701396202143
4	0.8892910707487633	2.9075077283542154
5	0.41984222965823936	1.7158253461279538
6	0.22188741433391304	1.0881803380593822
7	0.13875259510500867	0.7938822819286244
8	0.08417909610601816	0.5504415383099368
9	0.05445553757801597	0.4005913007869547
>10	0.1708221597554192	2.0704485663356618
>50	0.002447611989891054	0.13219230506404692
>100	5.579988909120605E-4	0.08571619916228938
>500	2.9117598368792008E-5	0.017306465856252173
>1k	3.8593167376361944E-5	0.042868831208179095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.558646111195128E-6
2	0.0	2.995526138918295E-5	0.0	0.0	8.558646111195128E-6
3	0.0	3.423458444478051E-5	0.0	8.558646111195128E-6	8.558646111195128E-6
4	0.0	9.414510722314642E-5	0.0	1.7117292222390256E-5	8.558646111195128E-6
5	0.0	9.842443027874398E-5	0.0	1.7117292222390256E-5	8.558646111195128E-6
6	4.279323055597564E-6	9.842443027874398E-5	0.0	3.8513907500378076E-5	1.2837969166792693E-5
7	4.279323055597564E-6	9.842443027874398E-5	0.0	4.279323055597564E-5	2.139661527798782E-5
8	4.279323055597564E-6	1.0698307638993911E-4	0.0	4.707255361157321E-5	2.995526138918295E-5
9	4.279323055597564E-6	2.396420911134636E-4	0.0	7.274849194515859E-5	3.423458444478051E-5
10-11	8.558646111195128E-6	2.396420911134636E-4	0.0	1.1340206097333545E-4	3.423458444478051E-5
12-13	8.558646111195128E-6	2.396420911134636E-4	0.0	1.3907799930692083E-4	4.065356902817686E-5
14-15	8.558646111195128E-6	3.423458444478051E-4	0.0	1.4121766083471961E-4	5.135187666717077E-5
16-17	2.139661527798782E-5	3.5732347514239667E-4	0.0	1.66893599168305E-4	5.99105227783659E-5
18-19	2.139661527798782E-5	3.915580595871772E-4	0.0	1.8615055291849403E-4	6.632950736176224E-5
20-21	2.139661527798782E-5	4.32211628615354E-4	0.0	2.1182649125207944E-4	7.274849194515859E-5
22-23	2.139661527798782E-5	4.835635052825248E-4	0.0	2.268041219466709E-4	7.274849194515859E-5
24-25	2.139661527798782E-5	5.263567358385004E-4	0.0	2.4178175264126238E-4	7.488815347295737E-5
26-27	2.139661527798782E-5	5.520326741720858E-4	0.0	2.995526138918295E-4	7.916747652855493E-5
28-29	2.139661527798782E-5	5.520326741720858E-4	0.0	5.49893012644287E-4	8.130713805635372E-5
30-31	2.139661527798782E-5	6.18362181533848E-4	0.0	0.0015234390077927327	8.130713805635372E-5
32-33	2.139661527798782E-5	6.247811661172443E-4	0.0	0.00367165918170271	9.414510722314642E-5
34-35	2.139661527798782E-5	7.317642425071835E-4	0.0	0.006521688336730688	9.414510722314642E-5
36-37	2.139661527798782E-5	8.387473188971226E-4	0.0	0.010634117793159948	9.842443027874398E-5
38-39	2.139661527798782E-5	8.729819033419031E-4	0.0	0.017337677359753532	1.0270375333434155E-4
40-41	2.139661527798782E-5	9.62847687509452E-4	0.0	0.0233672435450905	1.1554172250113423E-4
42-43	2.139661527798782E-5	0.0010035012565376288	0.0	0.03007936175779528	1.2196070708453058E-4
44-45	2.139661527798782E-5	0.0011169033175109644	0.0	0.03721085362994862	1.326590147235245E-4
46-47	2.139661527798782E-5	0.001189651809456123	0.0	0.04663606265990226	1.326590147235245E-4
48-49	2.139661527798782E-5	0.0011917914709839218	0.0	0.05501069787970669	1.3693833777912205E-4
50-51	2.139661527798782E-5	0.0012944952243182633	0.0	0.06459424186271744	1.3693833777912205E-4
52-53	2.139661527798782E-5	0.0013051935319572572	0.0	0.07276132991432538	1.4763664541811596E-4
54-55	2.139661527798782E-5	0.0013223108241796474	0.0	0.08140770214816026	1.5833495305710987E-4
56-57	2.139661527798782E-5	0.0013437074394576352	0.0	0.0892495616475428	1.775919068072989E-4
58-59	2.139661527798782E-5	0.001354405747096629	0.0	0.09724119745387125	1.925695375018904E-4
60-61	2.139661527798782E-5	0.001384361008485812	0.0	0.10496109624616926	1.925695375018904E-4
62-63	2.139661527798782E-5	0.001403617962236001	0.0	0.11137366184498221	2.0112818361308553E-4
64-65	2.139661527798782E-5	0.0015533942691819157	0.0	0.11693250249420345	2.0112818361308553E-4
66-67	2.5675938333585386E-5	0.0015662322383487087	0.0	0.12228379597522819	2.032678451408843E-4
68-69	2.5675938333585386E-5	0.0016090254689046842	0.0	0.12761797216403056	2.1396615277987822E-4
70-71	2.995526138918295E-5	0.001630422084182672	0.0	0.1326269198006075	2.1824547583547578E-4
72-73	2.995526138918295E-5	0.0016432600533494647	0.0	0.1368527513180101	2.460610756968599E-4
74-75	2.995526138918295E-5	0.001707449899183428	0.0	0.13994028290262375	2.524800602802563E-4
76-77	2.995526138918295E-5	0.0018015950064065745	0.0	0.14271114458112316	2.524800602802563E-4
78-79	2.995526138918295E-5	0.0018572262061293428	0.0	0.1445854880794749	2.5461972180805504E-4
80-81	2.995526138918295E-5	0.0018572262061293428	0.0	0.14516533635350837	2.610387063914514E-4
82-83	2.995526138918295E-5	0.0018572262061293428	0.0	0.14559326865906813	2.738766755582441E-4
84-85	2.995526138918295E-5	0.0018615055291849405	0.0	0.1458093744733758	2.802956601416404E-4
86-87	2.995526138918295E-5	0.001895740113629721	0.0	0.14593347484198815	2.9313362930843316E-4
88	2.995526138918295E-5	0.0019770472516860748	0.0	0.14595059213421052	3.081112600030246E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27170	0.0	45.956142	1
GTATCAA	47855	0.0	35.530346	1
ATCAACG	59225	0.0	28.031471	3
TCAACGC	59550	0.0	27.892593	4
CAACGCA	60495	0.0	27.40834	5
AACGCAG	62005	0.0	26.788282	6
TATCAAC	63085	0.0	26.519278	2
ACGCAGA	70830	0.0	23.379467	7
CGCAGAG	71350	0.0	23.214964	8
GTACATG	64010	0.0	20.262337	1
TACATGG	63655	0.0	19.69484	2
ACATGGG	64055	0.0	19.203945	3
GCAGAGT	85360	0.0	19.17857	9
AGAGTAC	77490	0.0	18.346333	10-11
GAGTACT	51090	0.0	17.57544	12-13
CAGAGTA	83915	0.0	16.999191	10-11
CATGGGG	43935	0.0	16.74741	4
AGTACTT	56720	0.0	16.130793	12-13
GTACTTT	59190	0.0	15.167841	14-15
GTGGTAT	14135	0.0	13.954042	1
>>END_MODULE