FastQCFastQC Report
Fri 27 May 2016
SRR2139257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23153514
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA281800.048.95671
GTATCAA506250.036.3489341
ATCAACG599650.029.9185663
TCAACGC626900.028.6180084
CAACGCA636050.028.1930965
AACGCAG645000.027.7824296
TATCAAC660100.027.4371622
ACGCAGA657700.027.2650457
CGCAGAG758700.023.5968978
GTACATG686950.020.8394371
TACATGG681700.020.3422222
ACATGGG670150.020.2016753
GCAGAGT904600.019.5923149
AGAGTAC813550.018.54192410-11
GAGTACT520350.018.357312-13
CAGAGTA879650.017.688610-11
CATGGGG480950.017.2807874
GTGGTAT131200.016.5777951
AGTACTT581550.016.05484412-13
GTACTTT607100.015.75586314-15
Produced by FastQC (version 0.11.5)