##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23153514
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82245710089622	31.0	31.0	34.0	30.0	34.0
2	31.924933036082557	31.0	31.0	34.0	30.0	34.0
3	32.646872003964496	34.0	31.0	34.0	30.0	37.0
4	35.57004690519115	37.0	35.0	37.0	33.0	37.0
5	35.45891755350829	37.0	35.0	37.0	33.0	37.0
6	35.466056210733285	37.0	35.0	37.0	33.0	37.0
7	35.448272171558926	37.0	35.0	37.0	33.0	37.0
8	35.70146017576425	37.0	35.0	37.0	33.0	39.0
9	36.95717047528941	39.0	37.0	39.0	33.0	39.0
10-11	36.86772571541408	39.0	37.0	39.0	32.0	39.0
12-13	36.95302836105137	39.0	37.0	39.0	32.0	39.5
14-15	37.95290758024894	40.0	37.0	41.0	33.0	41.0
16-17	37.92544177095537	40.0	37.0	41.0	33.0	41.0
18-19	37.95324590902271	40.0	37.0	41.0	32.5	41.0
20-21	37.925477848515094	40.0	37.0	41.0	32.0	41.0
22-23	37.825295447144185	40.0	37.0	41.0	32.0	41.0
24-25	37.71027765451986	40.0	37.0	41.0	32.0	41.0
26-27	37.52882875310095	39.0	36.0	41.0	32.0	41.0
28-29	37.40343770002571	39.0	36.0	41.0	31.5	41.0
30-31	37.22581288220087	39.0	36.0	41.0	31.0	41.0
32-33	37.03504321834658	39.0	36.0	40.5	30.5	41.0
34-35	36.906443190387236	39.0	35.5	40.0	30.0	41.0
36-37	36.757409664556604	38.5	35.0	40.0	30.0	41.0
38-39	36.61006439796677	38.0	35.0	40.0	30.0	41.0
40-41	36.48555269818564	38.0	35.0	40.0	30.0	41.0
42-43	36.297497634080415	38.0	35.0	40.0	30.0	41.0
44-45	36.17047586664597	38.0	35.0	40.0	29.5	41.0
46-47	36.33762708142274	38.0	35.0	40.0	30.0	41.0
48-49	36.72059352033991	39.0	35.0	40.0	30.0	41.0
50-51	36.79602384730444	39.0	35.0	41.0	30.0	41.0
52-53	36.75194829790353	39.0	35.0	40.5	30.0	41.0
54-55	36.640177978196526	39.0	35.0	40.0	30.0	41.0
56-57	36.49789762337339	38.5	35.0	40.0	30.0	41.0
58-59	36.30492250059902	38.0	34.5	40.0	29.0	41.0
60-61	36.13493081793004	38.0	34.0	40.0	29.0	41.0
62-63	35.92277236548076	38.0	34.0	40.0	28.5	41.0
64-65	35.68000294049134	37.0	34.0	40.0	28.0	41.0
66-67	35.42481827249007	37.0	34.0	40.0	28.0	41.0
68-69	35.14884002968735	36.5	33.5	39.0	28.0	41.0
70-71	34.86819162461647	36.0	33.0	39.0	28.0	41.0
72-73	34.54678382998658	35.5	33.0	39.0	28.0	40.0
74-75	34.01732081204947	35.0	32.0	37.5	27.0	39.5
76-77	32.67194474852495	34.0	30.5	36.0	26.0	39.0
78-79	33.57083616380297	35.0	32.0	37.0	27.0	39.0
80-81	33.555201647228174	35.0	32.0	36.5	27.0	39.0
82-83	33.32526049346937	35.0	32.0	36.0	27.0	37.5
84-85	33.109902544086616	35.0	32.0	36.0	27.0	37.0
86-87	32.90249652935183	34.5	32.0	35.0	27.0	37.0
88-89	32.756387027929755	34.0	32.0	35.0	27.0	36.0
90-91	32.62984185142858	34.0	32.0	35.0	27.0	36.0
92-93	32.55433685686693	34.0	32.0	35.0	27.0	36.0
94-95	32.544653370761765	34.0	32.0	35.0	27.0	35.5
96-97	32.62368275008681	34.0	32.0	35.0	27.5	35.0
98-99	32.61413493197021	34.0	31.5	35.0	28.0	35.0
100	31.34177043740237	33.0	30.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	13.0
19	45.0
20	306.0
21	1846.0
22	8349.0
23	27761.0
24	69595.0
25	140205.0
26	239226.0
27	361179.0
28	493567.0
29	628877.0
30	768580.0
31	919024.0
32	1096484.0
33	1324985.0
34	1650599.0
35	2108123.0
36	2875348.0
37	4035280.0
38	4315987.0
39	2004890.0
40	83240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.001372479589506	5.184385253741959E-5	26.317507824533443	29.681067852024512
2	14.680943581059012	26.15597180053879	37.63800003346517	21.525084584937034
3	16.012531497941758	26.25534276744459	34.151441572735216	23.58068416187844
4	12.803259053329022	25.024842926365427	38.04150841826228	24.13038960204327
5	16.37968215105491	37.72048597029375	32.923451705862014	12.976380172789323
6	26.156431373656712	38.7984087426211	21.529064659472425	13.51609522424976
7	24.609466390694145	33.55382399206548	24.04431673068359	17.792392886556783
8	23.519459012548115	32.646389340572206	23.1852289542412	20.64892269263848
9	23.33930996547046	20.732067900898645	24.72948375684583	31.19913837678506
10-11	23.946395383745802	26.57343335395945	29.755139300591864	19.72503196170288
12-13	24.39076286722032	24.756478554758772	29.34648771319936	21.50627086482155
14-15	21.815332321284814	27.073141557214814	27.32422812152676	23.78729799997361
16-17	20.957795115091717	29.220336783992597	28.24729453803346	21.574573562882225
18-19	21.116051809888745	28.432659950473997	29.747258459252045	20.70402978038521
20-21	21.908071149115603	27.86161713066708	29.47131773255765	20.758993987659665
22-23	21.73040085188678	27.64253406508135	29.286197919557978	21.3408671634739
24-25	21.434935599910744	27.877610887208515	29.441963558873752	21.24548995400699
26-27	21.43023005515128	28.063598192256407	29.228418281432816	21.2777534711595
28-29	21.44265598868643	27.939414249285495	29.257964748652217	21.35996501337586
30-31	21.623593949599996	27.892972207449628	29.235245431616995	21.248188411333377
32-33	21.526946839011153	28.166475324410516	29.095578216481577	21.21099962009675
34-35	21.633028881500405	27.998476531144938	29.072566102334097	21.295928485020557
36-37	21.57810232808285	28.04870121432284	29.10258023980549	21.27061621778882
38-39	21.45920034914012	28.234187638059428	29.069553298068996	21.237058714731457
40-41	21.730032339231308	27.93424430961011	28.87439033101137	21.461333020147215
42-43	21.58936322497384	28.037633419734686	28.878362627415772	21.4946407278757
44-45	21.672115941155727	28.36104108763263	28.524727913807375	21.44211505740427
46-47	21.799909340823024	28.207988875738486	28.249950928071776	21.742150855366717
48-49	21.67771331760856	28.295965125034815	28.346611725413677	21.679709831942944
50-51	21.683663325150256	28.566647640827668	28.089550870331216	21.660138163690856
52-53	21.870951053278695	28.256702081952938	28.09723857126719	21.775108293501173
54-55	21.760066605526717	28.21278353121291	28.258632599005516	21.768517264254857
56-57	21.785299530245553	28.52167762417846	28.051364189880644	21.64165865569534
58-59	21.868627969160638	28.338243384240315	28.094862073079412	21.698266573519636
60-61	21.794006925864544	28.39219235323267	28.139372114425136	21.67442860647765
62-63	21.76937590161204	28.566163550869746	28.045322720931853	21.619137826586364
64-65	21.81865051326504	28.342415848883572	28.039707617393937	21.799226020457454
66-67	21.776097472758718	28.412623534307208	28.008569099574494	21.802709893359587
68-69	21.779094055531463	28.502877666555943	28.02868502181568	21.689343256096915
70-71	21.831867041003253	28.13057914164483	28.09243304110966	21.945120776242256
72-73	21.788751871978313	28.271741582602782	28.059530879684207	21.879975665734698
74-75	21.63177395982213	28.43180880140917	28.12287448945292	21.813542749315772
76-77	21.87656680781305	28.234226952683212	27.985921443936252	21.903284795567487
78-79	21.68016572805727	28.34229586582599	28.104232384655436	21.873306021461303
80-81	21.760313966976387	28.377218053263668	28.01207232734506	21.850395652414882
82-83	21.86043987420238	28.15470147564031	28.151087106622896	21.833771543534418
84-85	21.83165889914763	28.211500687796747	28.1898874804508	21.76695293260483
86-87	21.7079568517032	28.314783805849668	28.169317605986283	21.807941736460847
88-89	21.815033254679868	28.13849821979956	28.127924732382997	21.918543793137584
90-91	21.8564052139095	28.11428823016029	28.2526191501014	21.776687405828806
92-93	21.741833047552323	28.145576408154927	28.293723039469782	21.818867504822975
94-95	21.712237199160235	28.070215693728624	28.338924980513212	21.87862212659793
96-97	21.65394421274428	28.40312749797938	28.53000046826441	21.41292782101193
98-99	22.683557678248867	30.45122095479753	28.157469522900563	18.707751844053035
100	22.182042363417114	30.561151514649644	22.446888676884928	24.809917445048317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4112.0
1	3893.0
2	6613.5
3	10041.0
4	13363.0
5	21821.0
6	29004.0
7	33892.5
8	37172.0
9	36163.0
10	32387.0
11	28127.0
12	29428.0
13	37194.5
14	58170.5
15	91573.5
16	120906.5
17	138060.5
18	141850.5
19	136230.5
20	126899.5
21	117946.5
22	115010.0
23	124590.0
24	148938.0
25	187279.5
26	237624.0
27	293730.5
28	350372.0
29	410930.0
30	468271.0
31	521880.5
32	581591.0
33	637222.0
34	688728.5
35	735562.5
36	782473.5
37	831063.5
38	864782.5
39	884662.5
40	909263.5
41	933899.0
42	945963.5
43	954847.0
44	960508.5
45	964550.5
46	961290.5
47	949624.0
48	935094.0
49	910040.5
50	881197.5
51	847531.5
52	799271.0
53	745055.5
54	693105.5
55	637939.5
56	581064.5
57	524143.5
58	463711.5
59	401689.5
60	337640.0
61	276514.5
62	221493.0
63	173043.5
64	131741.5
65	98405.0
66	73945.5
67	57174.0
68	44615.0
69	32797.0
70	23571.5
71	17382.0
72	12627.5
73	8929.0
74	6315.0
75	4565.5
76	3221.0
77	2179.5
78	1503.5
79	1055.0
80	769.5
81	578.5
82	460.5
83	346.0
84	243.0
85	164.5
86	115.0
87	97.5
88	74.5
89	54.5
90	40.5
91	30.0
92	21.0
93	11.0
94	6.5
95	6.5
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030604425747210555
2	0.10791882389860993
3	7.342298020075916E-5
4	1.8139795108422852E-4
5	0.0
6	0.0
7	4.318998835338774E-6
8	4.318998835338774E-6
9	2.5913993012032642E-5
10-11	9.933697321279181E-5
12-13	3.887098951804897E-5
14-15	6.240953317064529E-4
16-17	0.0013194541441959955
18-19	8.206097787143671E-5
20-21	5.507081123689021E-4
22-23	0.002207516199540111
24-25	5.83398261598503E-4
26-27	0.0048363459026367255
28-29	0.004050536063132105
30-31	0.009507450768330754
32-33	0.008401752648621561
34-35	0.0039100896463245445
36-37	0.006979424119133952
38-39	2.2684541997665517E-4
40-41	5.078308402608999E-4
42-43	0.0017632770009165688
44-45	9.020172284839632E-6
46-47	2.2784407217507812E-5
48-49	7.360416091682078E-5
50-51	2.7840420011712463E-5
52-53	5.542987422774432E-4
54-55	0.0021456290294647917
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.4478708162613722E-6
64-65	9.88523709283975E-6
66-67	1.997179383556603E-5
68-69	2.3220062921827463E-4
70-71	6.229146400666458E-4
72-73	3.5382960630232E-4
74-75	6.505322987065615E-4
76-77	0.0019846499089452665
78-79	0.006371734248895127
80-81	6.636555845303671E-4
82-83	4.11970455236691E-4
84-85	0.0
86-87	0.0
88-89	2.8701105084389285E-6
90-91	5.554633681263893E-5
92-93	3.134263053265338E-4
94-95	8.26034629023718E-5
96-97	5.718286699646355E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	910.0
20-21	3092.0
22-23	6180.0
24-25	12520.0
26-27	16863.0
28-29	29329.0
30-31	56195.0
32-33	66495.0
34-35	94340.0
36-37	126950.0
38-39	153735.0
40-41	167455.0
42-43	192848.0
44-45	227523.0
46-47	212887.0
48-49	190408.0
50-51	175528.0
52-53	169620.0
54-55	182312.0
56-57	193940.0
58-59	199383.0
60-61	200818.0
62-63	193257.0
64-65	199175.0
66-67	216263.0
68-69	223222.0
70-71	227284.0
72-73	221260.0
74-75	222361.0
76-77	213175.0
78-79	236924.0
80-81	253151.0
82-83	249419.0
84-85	252356.0
86-87	270814.0
88-89	308157.0
90-91	343263.0
92-93	389142.0
94-95	505514.0
96-97	1310675.0
98-99	4132678.0
100-101	1.0506093E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.8453282571497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.25684068179856	70.54307927601819
2	8.296141368480196	13.414085444050011
3	2.320008135613313	5.626854578487485
4	0.9531402318312944	3.08227739669987
5	0.4589543731637673	1.855215847673957
6	0.24440042934997355	1.1855179762192163
7	0.14079099576979923	0.7967605988062275
8	0.09535978722226213	0.6167514640412585
9	0.05486333734708092	0.39919000653967474
>10	0.17468999430647977	2.090643820860501
>50	0.003505169491886328	0.18833345556394243
>100	0.0012530782086257462	0.15663999713528529
>500	3.146960069782908E-5	0.021957038480251097
>1k	2.094781603490804E-5	0.022693099424134944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.637997670677548E-6
2	0.0	6.0465983694742834E-5	0.0	0.0	8.637997670677548E-6
3	0.0	6.0465983694742834E-5	0.0	0.0	8.637997670677548E-6
4	0.0	6.0465983694742834E-5	0.0	4.318998835338774E-6	8.637997670677548E-6
5	0.0	6.0465983694742834E-5	0.0	4.318998835338774E-6	8.637997670677548E-6
6	4.7508987188726516E-5	6.0465983694742834E-5	0.0	4.318998835338774E-5	2.159499417669387E-5
7	4.7508987188726516E-5	6.0465983694742834E-5	0.0	4.318998835338774E-5	2.159499417669387E-5
8	5.182798602406529E-5	7.774197903609793E-5	0.0	4.7508987188726516E-5	2.5913993012032646E-5
9	5.182798602406529E-5	1.2525096622482444E-4	0.0	5.182798602406529E-5	3.0232991847371417E-5
10-11	5.398748544173468E-5	1.2525096622482444E-4	0.0	5.182798602406529E-5	3.0232991847371417E-5
12-13	5.6146984859404066E-5	1.2741046564249383E-4	0.0	8.637997670677548E-5	3.2392491265040804E-5
14-15	6.0465983694742834E-5	2.2458793943761624E-4	0.0	9.069897554211426E-5	3.887098951804897E-5
16-17	9.501797437745303E-5	2.461829336143101E-4	0.0	9.717747379512242E-5	3.887098951804897E-5
18-19	9.501797437745303E-5	2.461829336143101E-4	0.0	1.2525096622482444E-4	4.7508987188726516E-5
20-21	9.501797437745303E-5	2.461829336143101E-4	0.0	1.4252696156617954E-4	4.7508987188726516E-5
22-23	1.014964726304612E-4	2.807349242970203E-4	0.0	1.5764345748986525E-4	5.398748544173467E-5
24-25	1.0365597204813058E-4	3.023299184737142E-4	0.0	1.835574505018979E-4	6.0465983694742834E-5
26-27	1.0365597204813058E-4	3.196059138150693E-4	0.0	2.8937292196769786E-4	6.910398136542038E-5
28-29	1.0365597204813058E-4	3.196059138150693E-4	0.0	6.435308264654773E-4	6.910398136542038E-5
30-31	1.0581547146579997E-4	3.7791239809214274E-4	0.0	0.0016995260417058075	7.126348078308977E-5
32-33	1.0797497088346936E-4	3.843908963451509E-4	0.0	0.0039043749471462516	7.774197903609793E-5
34-35	1.1229396971880813E-4	4.29740384116208E-4	0.0	0.007005416110919491	9.069897554211426E-5
36-37	1.1229396971880813E-4	4.750898718872651E-4	0.0	0.011209961477121787	9.069897554211426E-5
38-39	1.1229396971880813E-4	4.815683701402733E-4	0.0	0.017876336179467184	9.069897554211426E-5
40-41	1.1661296855414689E-4	5.441938532526855E-4	0.0	0.02370698460717453	9.501797437745303E-5
42-43	1.1661296855414689E-4	5.938623398590814E-4	0.0	0.0300084039079338	1.014964726304612E-4
44-45	1.1661296855414689E-4	6.435308264654773E-4	0.0	0.03656032514114273	1.1229396971880812E-4
46-47	1.1661296855414689E-4	6.435308264654773E-4	0.0	0.04515297332404922	1.2741046564249383E-4
48-49	1.2093196738948567E-4	6.435308264654773E-4	0.0	0.05275009227541012	1.4684596040151832E-4
50-51	1.2525096622482444E-4	7.061563095778896E-4	0.0	0.06138593044667	1.4684596040151832E-4
52-53	1.2525096622482444E-4	7.104753084132283E-4	0.0	0.06861161549819177	1.6196245632520403E-4
54-55	1.2525096622482444E-4	7.169538066662365E-4	0.0	0.07709412921079711	1.7707895224888974E-4
56-57	1.2525096622482444E-4	7.342298020075916E-4	0.0	0.08507563905850317	1.7923845166655913E-4
58-59	1.2525096622482444E-4	7.407083002605998E-4	0.0	0.09361861875480326	1.8139795108422852E-4
60-61	1.2525096622482444E-4	7.558247961842855E-4	0.0	0.1021529604534327	1.8139795108422852E-4
62-63	1.3820796273084077E-4	7.774197903609794E-4	0.0	0.10908927258298676	1.9867394642558361E-4
64-65	1.4252696156617954E-4	8.897137600797875E-4	0.0	0.11513587095246104	1.9867394642558361E-4
66-67	1.4684596040151832E-4	9.069897554211426E-4	0.0	0.12122565931028871	2.0515244467859178E-4
68-69	1.4684596040151832E-4	9.437012455215221E-4	0.0	0.12693969476944192	2.1163094293159994E-4
70-71	1.490054598191877E-4	9.588177414452078E-4	0.0	0.1318503964452221	2.202689406022775E-4
72-73	1.5980295690753464E-4	9.696152385335547E-4	0.0	0.13571374090343263	2.461829336143101E-4
74-75	1.5980295690753464E-4	0.0010128052268869425	0.0	0.13909767649092056	2.461829336143101E-4
76-77	1.6412195574287342E-4	0.001071111711164016	0.0	0.14214257066983438	2.505019324496489E-4
78-79	1.684409545782122E-4	0.0010840687076700323	0.0	0.14390904119348796	2.505019324496489E-4
80-81	1.684409545782122E-4	0.0010840687076700323	0.0	0.14452233902810607	2.612994295379958E-4
82-83	1.684409545782122E-4	0.0010862282070877017	0.0	0.14496935540756362	2.7209692662634276E-4
84-85	1.7275995341355097E-4	0.0011013447030113874	0.0	0.14517234835282455	2.7209692662634276E-4
86-87	1.7275995341355097E-4	0.0011229396971880812	0.0	0.14526520682778432	2.7209692662634276E-4
88	1.7275995341355097E-4	0.0011747676832121465	0.0	0.145273844825455	2.7209692662634276E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28180	0.0	48.9567	1
GTATCAA	50625	0.0	36.348934	1
ATCAACG	59965	0.0	29.918566	3
TCAACGC	62690	0.0	28.618008	4
CAACGCA	63605	0.0	28.193096	5
AACGCAG	64500	0.0	27.782429	6
TATCAAC	66010	0.0	27.437162	2
ACGCAGA	65770	0.0	27.265045	7
CGCAGAG	75870	0.0	23.596897	8
GTACATG	68695	0.0	20.839437	1
TACATGG	68170	0.0	20.342222	2
ACATGGG	67015	0.0	20.201675	3
GCAGAGT	90460	0.0	19.592314	9
AGAGTAC	81355	0.0	18.541924	10-11
GAGTACT	52035	0.0	18.3573	12-13
CAGAGTA	87965	0.0	17.6886	10-11
CATGGGG	48095	0.0	17.280787	4
GTGGTAT	13120	0.0	16.577795	1
AGTACTT	58155	0.0	16.054844	12-13
GTACTTT	60710	0.0	15.755863	14-15
>>END_MODULE