FastQCFastQC Report
Fri 27 May 2016
SRR2139256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23799134
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279950.046.909221
GTATCAA501700.036.0626871
ATCAACG605300.029.0358723
TCAACGC608000.028.9069274
CAACGCA616950.028.4671155
AACGCAG632700.027.811696
TATCAAC655250.027.1358722
ACGCAGA730250.024.0216127
CGCAGAG736300.023.8299478
GCAGAGT880200.019.6998839
GTACATG640900.019.0830761
AGAGTAC793050.018.80333110-11
TACATGG633650.018.7094732
GAGTACT533000.018.32400712-13
ACATGGG635950.018.1993033
CAGAGTA864150.017.37313510-11
AGTACTT585050.016.51032812-13
GTACTTT616050.015.86845114-15
CATGGGG435850.015.8362324
GTGGTAT145200.015.4123611
Produced by FastQC (version 0.11.5)