FastQCFastQC Report
Fri 27 May 2016
SRR2139255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19205399
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225550.049.2930681
GTATCAA405450.035.7631261
ATCAACG491300.028.7622573
TCAACGC492700.028.6890564
CAACGCA501200.028.1857455
AACGCAG516400.027.461866
TATCAAC520850.027.3467562
ACGCAGA592650.023.8364937
CGCAGAG599100.023.5588288
GTACATG533700.019.413271
GCAGAGT719800.019.3868459
TACATGG528800.019.131772
ACATGGG533900.018.5208233
GAGTACT418600.018.46977612-13
AGAGTAC644050.018.3669710-11
CAGAGTA702500.017.58923710-11
CATGGGG374300.016.9124764
GTACTTT487300.015.92314-15
AGTACTT473550.015.71892812-13
GTGGTAT103250.015.4411851
Produced by FastQC (version 0.11.5)