##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19205399
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.674460082813173	31.0	31.0	34.0	30.0	34.0
2	31.810254189459954	31.0	31.0	34.0	30.0	34.0
3	31.856580068969148	31.0	31.0	34.0	30.0	34.0
4	35.6058467725664	37.0	35.0	37.0	33.0	37.0
5	35.4583934444684	37.0	35.0	37.0	33.0	37.0
6	35.477910091844485	37.0	35.0	37.0	33.0	37.0
7	35.38592736344608	37.0	35.0	37.0	33.0	37.0
8	35.37077407243661	37.0	35.0	37.0	32.0	37.0
9	36.98208347558934	39.0	37.0	39.0	33.0	39.0
10-11	36.86745386024003	39.0	37.0	39.0	32.5	39.0
12-13	36.79931408871016	39.0	37.0	39.0	32.0	39.0
14-15	37.95677098403423	40.0	37.0	41.0	33.0	41.0
16-17	37.934201262884464	40.0	37.0	41.0	33.0	41.0
18-19	37.95535651198915	40.0	37.0	41.0	33.0	41.0
20-21	37.93611301912677	40.0	37.0	41.0	32.0	41.0
22-23	37.83985965982719	40.0	37.0	41.0	32.0	41.0
24-25	37.73488007685211	40.0	37.0	41.0	32.0	41.0
26-27	37.549262374902845	39.0	36.5	41.0	32.0	41.0
28-29	37.42142857528249	39.0	36.0	41.0	31.5	41.0
30-31	37.245677507574186	39.0	36.0	41.0	31.0	41.0
32-33	37.05306974575923	39.0	36.0	40.5	31.0	41.0
34-35	36.9290127261553	39.0	35.5	40.0	30.0	41.0
36-37	36.779083040599176	39.0	35.0	40.0	30.0	41.0
38-39	36.63173273606657	38.0	35.0	40.0	30.0	41.0
40-41	36.50568081925463	38.0	35.0	40.0	30.0	41.0
42-43	36.31914585764659	38.0	35.0	40.0	30.0	41.0
44-45	36.16075188668286	38.0	35.0	40.0	29.5	41.0
46-47	36.2927817919699	38.0	35.0	40.0	30.0	41.0
48-49	36.70690168026782	39.0	35.0	40.0	30.0	41.0
50-51	36.80808691082771	39.0	35.0	41.0	30.0	41.0
52-53	36.76827194326066	39.0	35.0	41.0	30.0	41.0
54-55	36.66469314797372	39.0	35.0	40.0	30.0	41.0
56-57	36.52921524627628	38.5	35.0	40.0	30.0	41.0
58-59	36.33585847885534	38.0	34.5	40.0	29.0	41.0
60-61	36.16901441243674	38.0	34.0	40.0	29.0	41.0
62-63	35.96539620500323	38.0	34.0	40.0	29.0	41.0
64-65	35.72246281261219	37.5	34.0	40.0	28.0	41.0
66-67	35.47030393797588	37.0	34.0	40.0	28.0	41.0
68-69	35.20018999254388	36.5	33.5	39.0	28.0	41.0
70-71	34.917802463611594	36.0	33.0	39.0	28.0	41.0
72-73	34.61170843227954	36.0	33.0	39.0	28.0	40.0
74-75	34.29421927632487	35.0	32.5	38.0	28.0	40.0
76-77	32.51117132617764	34.0	30.5	36.0	25.5	39.0
78-79	33.564679844771426	35.0	32.0	37.0	27.0	39.0
80-81	33.59334987403149	35.0	32.0	36.5	27.5	39.0
82-83	33.36212335819701	35.0	32.0	36.0	27.0	38.5
84-85	33.143614945548904	35.0	32.0	36.0	27.0	37.0
86-87	32.92647538034488	34.5	32.0	35.0	27.0	37.0
88-89	32.77201100201034	34.0	32.0	35.0	27.0	36.5
90-91	32.642022507035904	34.0	32.0	35.0	27.0	36.0
92-93	32.55721402747359	34.0	32.0	35.0	27.0	36.0
94-95	32.531571876923124	34.0	32.0	35.0	27.0	35.5
96-97	32.61087717908752	34.0	32.0	35.0	27.5	35.0
98-99	32.753246089044104	34.0	32.0	35.0	29.0	35.0
100	31.36804255545189	33.0	30.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	26.0
20	216.0
21	1464.0
22	6726.0
23	22918.0
24	56959.0
25	115321.0
26	198159.0
27	299172.0
28	409764.0
29	524619.0
30	641009.0
31	766572.0
32	912047.0
33	1102993.0
34	1373812.0
35	1755962.0
36	2391059.0
37	3365659.0
38	3592296.0
39	1618412.0
40	50228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6906619852053	21.764317419284026	14.946401269767945	23.598619325742725
2	14.25125208707249	22.820312117904486	41.168467563470415	21.759968231552605
3	17.310075151263455	28.15823821207776	31.49423763598975	23.03744900066903
4	12.212244094611187	19.812916615899535	40.15419149049171	27.820647798997573
5	12.314672556399376	38.8932247645571	36.47426955305641	12.317833125987125
6	27.742188537712753	40.161654543079266	19.740438613121235	12.355718306086741
7	24.640060849555898	33.570117444579	24.93942979263279	16.850391913232315
8	23.548820828976268	37.39817641903716	22.233971811780634	16.819030940205927
9	25.348299266950768	16.784153619334568	21.94425576771527	35.923291345999395
10-11	23.202921805829128	27.41114040787419	30.134212892291707	19.25172489400498
12-13	24.383103760284055	25.069993995177658	29.925324378211904	20.62157786632638
14-15	21.549101686205198	26.23789571897009	27.534943595255147	24.678058999569576
16-17	21.037795871479258	29.31944801916284	28.1001564845814	21.5425996247765
18-19	21.104126009153106	28.622217862336992	29.648450148014373	20.625205980495537
20-21	21.963991605697633	27.837615765266964	29.404313370451746	20.794079258583658
22-23	21.62482712739259	27.811583889758595	29.305619343743306	21.257969639105507
24-25	21.41125568752739	27.954112960627043	29.35740041169656	21.27723094014901
26-27	21.431346155630553	28.073711719024736	29.19112059340614	21.303821531938574
28-29	21.3874264861061	28.12125495534332	29.20809373198678	21.2832248265638
30-31	21.697347075434138	27.958205269361052	29.141202118084802	21.203245537120008
32-33	21.501916989569512	28.175066680061516	29.02552096138711	21.297495368981863
34-35	21.55556249007759	28.170022187318462	29.10895384434667	21.16546147825728
36-37	21.599196693329247	28.134523056975873	28.997411400915045	21.268868848779828
38-39	21.478691389082492	28.208166972084207	29.051021223048018	21.26212041578528
40-41	21.674222592378165	28.07209930352125	28.92659692261924	21.327081181481347
42-43	21.598186761761703	28.215383691759193	28.741457539111682	21.444972007367426
44-45	21.680114248655418	28.31425952491074	28.55166102147339	21.453965204960454
46-47	21.764913168054942	28.29046074669494	28.260051257946685	21.684574827303436
48-49	21.698071691354297	28.365669459889187	28.29753451592133	21.638724332835185
50-51	21.679658404824185	28.477042178137125	28.176141080999805	21.667158336038888
52-53	21.78550336883523	28.37711029663721	28.138380105511416	21.699006229016142
54-55	21.768464528130696	28.307923110622962	28.188900774753627	21.734711586492715
56-57	21.78462114800947	28.378731364063125	28.145767389744965	21.69088009818244
58-59	21.770853549024913	28.497259257915026	28.12917233091321	21.60271486214685
60-61	21.828097207694324	28.44679038059592	28.110032990711787	21.61507942099797
62-63	21.759520953004014	28.514931336852527	28.059154492302735	21.666393217840724
64-65	21.742342581775414	28.489317715324603	28.06043313132519	21.707906571574792
66-67	21.82322463015324	28.484367569570225	27.95319536148668	21.739212438789853
68-69	21.743122131688782	28.443250652187118	28.054632397650238	21.758994818473866
70-71	21.782910690949283	28.203141168018064	28.12575108621455	21.888197054818097
72-73	21.792857349538654	28.286460450321933	28.084608012724626	21.836074187414788
74-75	21.66915715857422	28.352069035035356	28.073536665019454	21.90523714137097
76-77	21.738598622430395	28.33130170522124	28.068460215145542	21.861639457202823
78-79	21.766665102517454	28.31580924150101	28.043743632796602	21.873782023184933
80-81	21.7680503745542	28.318364695141163	28.05691113707791	21.856673793226726
82-83	21.78687557855374	28.261139375357608	28.15930435725117	21.792680688837486
84-85	21.871157712816082	28.24414619231501	28.091384346138838	21.793311748730066
86-87	21.707151542601487	28.266073798988433	28.192910626105522	21.833864032304554
88-89	21.800944817996502	28.21424223913511	28.159752564510416	21.825060378357968
90-91	21.78452866385074	28.188710414253887	28.279282878739714	21.747478043155656
92-93	21.815847196739817	28.122966439543912	28.261214605775304	21.79997175794097
94-95	21.608622042795876	28.159236160126138	28.383614598567057	21.84852719851093
96-97	21.643279664994182	28.31313801395556	28.47535501487417	21.568227306176095
98-99	22.680298108828094	30.269700737860898	28.771141710445992	18.278859442865013
100	21.989143311637545	30.972399633654057	22.58779771400988	24.450659340698515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3033.0
1	2975.0
2	5184.5
3	7856.0
4	10574.5
5	17292.5
6	23034.5
7	26934.0
8	29437.0
9	28607.5
10	25695.0
11	22531.5
12	23658.5
13	30499.0
14	48159.5
15	73912.0
16	94787.5
17	106618.0
18	110373.0
19	107808.5
20	102020.5
21	96703.0
22	96174.0
23	105712.5
24	126943.5
25	160105.0
26	204058.5
27	250563.0
28	296386.5
29	344999.5
30	390281.5
31	433519.0
32	482810.0
33	531163.5
34	575939.5
35	617958.5
36	662323.0
37	703576.5
38	727364.5
39	742788.5
40	762305.5
41	777579.5
42	782917.5
43	788775.5
44	794833.5
45	799921.5
46	799143.0
47	790931.0
48	777684.0
49	753796.5
50	727507.5
51	696293.5
52	654447.0
53	611001.5
54	567885.5
55	521094.0
56	473031.5
57	423921.5
58	374293.5
59	324309.5
60	270229.5
61	219473.5
62	175126.0
63	135890.0
64	103272.0
65	77734.5
66	58464.5
67	44463.5
68	34112.0
69	24973.0
70	18002.5
71	13350.0
72	9599.0
73	6771.5
74	4778.0
75	3361.0
76	2365.0
77	1615.0
78	1085.5
79	753.0
80	569.0
81	432.5
82	328.0
83	222.0
84	149.5
85	111.0
86	84.0
87	64.5
88	45.0
89	28.5
90	18.5
91	14.0
92	11.0
93	6.5
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.13861206424297667
3	0.0
4	3.072052811816094E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	2.0827476690278602E-5
10-11	8.330990676111441E-5
12-13	7.810303758854477E-6
14-15	2.889812390776156E-4
16-17	0.0016245431818417311
18-19	2.6034345862848252E-6
20-21	4.061591536205613E-4
22-23	0.0022862969231521183
24-25	0.0
26-27	0.004475033833158385
28-29	0.0033046919740169307
30-31	0.00889134953707779
32-33	0.009408857435017673
34-35	0.003052147222343777
36-37	0.007461990338344684
38-39	2.149017032976348E-4
40-41	2.9385927875232053E-5
42-43	0.0022533053324026494
44-45	2.1728120536314114E-5
46-47	0.0
48-49	1.0800991564259419E-4
50-51	0.0
52-53	5.6316905363523515E-6
54-55	0.00261705472687202
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.946983533843317E-6
66-67	0.0
68-69	2.1855631110944497E-4
70-71	5.003379429805671E-4
72-73	4.8121965774229803E-4
74-75	4.8037749005084846E-4
76-77	0.001114556923137011
78-79	0.006998034127283162
80-81	8.098298118257984E-4
82-83	4.153553990119882E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.965693253487343E-4
94-95	1.170446104193917E-4
96-97	4.530777229914517E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	660.0
20-21	2368.0
22-23	4919.0
24-25	10164.0
26-27	13098.0
28-29	20917.0
30-31	47238.0
32-33	53872.0
34-35	73183.0
36-37	101672.0
38-39	127743.0
40-41	139080.0
42-43	157210.0
44-45	185885.0
46-47	173832.0
48-49	156681.0
50-51	145565.0
52-53	139815.0
54-55	148755.0
56-57	159282.0
58-59	163495.0
60-61	166047.0
62-63	158817.0
64-65	163204.0
66-67	175531.0
68-69	181066.0
70-71	185115.0
72-73	180110.0
74-75	182904.0
76-77	171200.0
78-79	191510.0
80-81	205972.0
82-83	202753.0
84-85	204871.0
86-87	219437.0
88-89	246013.0
90-91	276663.0
92-93	312429.0
94-95	373195.0
96-97	792950.0
98-99	1370182.0
100-101	1.1219996E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.32727825407787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.26624188986501	72.66719456508662
2	7.764376031231078	12.784398919849075
3	2.116235981631024	5.226718455330868
4	0.8375317130703755	2.758068255542373
5	0.39202520819000863	1.613718419863582
6	0.19938431441171484	0.9848860759243078
7	0.12306156338054275	0.7091926499568247
8	0.08102755041479252	0.5336622151395958
9	0.05354375156360823	0.39673002003699526
>10	0.1636910432860815	2.0454450822638734
>50	0.0021160685004296114	0.11441556292761729
>100	6.837223640825719E-4	0.08559991365547952
>500	4.6398638118943135E-5	0.030514842772192026
>1k	3.476345318273848E-5	0.04945502165061279
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.2068691725696505E-6
2	0.0	2.6034345862848254E-5	0.0	0.0	5.2068691725696505E-6
3	0.0	2.6034345862848254E-5	0.0	5.2068691725696505E-6	5.2068691725696505E-6
4	0.0	8.330990676111441E-5	0.0	3.6448084207987555E-5	5.2068691725696505E-6
5	0.0	8.851677593368406E-5	0.0	3.6448084207987555E-5	5.2068691725696505E-6
6	2.0827476690278602E-5	8.851677593368406E-5	0.0	3.6448084207987555E-5	1.0413738345139301E-5
7	2.0827476690278602E-5	8.851677593368406E-5	0.0	5.727556089826616E-5	1.0413738345139301E-5
8	2.0827476690278602E-5	1.0934425262396267E-4	0.0	5.727556089826616E-5	2.6034345862848254E-5
9	2.0827476690278602E-5	1.9265415938507708E-4	0.0	1.0413738345139302E-4	2.6034345862848254E-5
10-11	2.0827476690278602E-5	2.0046446314393154E-4	0.0	1.0674081803767785E-4	2.6034345862848254E-5
12-13	2.0827476690278602E-5	2.1087820148907088E-4	0.0	1.431889022456654E-4	2.6034345862848254E-5
14-15	2.6034345862848254E-5	2.9418810825018526E-4	0.0	1.4839577141823504E-4	3.1241215035417906E-5
16-17	4.686182255312686E-5	3.046018465953246E-4	0.0	1.822404210399378E-4	4.686182255312686E-5
18-19	4.686182255312686E-5	3.3323962704445763E-4	0.0	2.0827476690278603E-4	4.946525713941168E-5
20-21	4.686182255312686E-5	3.6968771125244523E-4	0.0	2.2910224359306463E-4	5.4672126311981336E-5
22-23	4.686182255312686E-5	4.3477357590956587E-4	0.0	2.9939497742275496E-4	6.248243007083581E-5
24-25	4.686182255312686E-5	5.024628751529713E-4	0.0	3.150155849404639E-4	6.508586465712063E-5
26-27	4.686182255312686E-5	5.311006556021044E-4	0.0	3.670842766661604E-4	6.768929924340547E-5
28-29	4.686182255312686E-5	5.415143939472436E-4	0.0	6.820998616066242E-4	7.029273382969029E-5
30-31	4.686182255312686E-5	5.831693473278009E-4	0.0	0.0015959054013925979	7.289616841597511E-5
32-33	4.686182255312686E-5	5.831693473278009E-4	0.0	0.003772376715526712	7.549960300225994E-5
34-35	4.686182255312686E-5	6.56065515743776E-4	0.0	0.006443500601054943	8.330990676111441E-5
36-37	4.946525713941168E-5	7.393754225048905E-4	0.0	0.010702719584216917	8.330990676111441E-5
38-39	5.206869172569651E-5	7.75823506712878E-4	0.0	0.01697439350257706	8.330990676111441E-5
40-41	5.206869172569651E-5	8.877711939231255E-4	0.0	0.023230446813419497	8.591334134739923E-5
42-43	5.206869172569651E-5	9.190124089585433E-4	0.0	0.030751769333196358	8.851677593368406E-5
44-45	6.508586465712063E-5	9.99718881133373E-4	0.0	0.03819238538079839	9.11202105199689E-5
46-47	7.810303758854477E-5	0.0010595978766179239	0.0	0.04820259136506354	9.893051427882336E-5
48-49	7.810303758854477E-5	0.0010674081803767783	0.0	0.05695013157498056	1.0413738345139302E-4
50-51	8.851677593368406E-5	0.001163735260069317	0.0	0.06706707837728339	1.0413738345139302E-4
52-53	8.851677593368406E-5	0.001176752433000741	0.0	0.07592916970899694	1.1194768721024749E-4
54-55	8.851677593368406E-5	0.0011897696059321651	0.0	0.08579097992184385	1.1975799096910197E-4
56-57	8.851677593368406E-5	0.0012027867788635893	0.0	0.09469212277235167	1.223614255553868E-4
58-59	8.851677593368406E-5	0.0012158039517950136	0.0	0.10344226641685497	1.2756829472795645E-4
60-61	1.0934425262396267E-4	0.0012548554705892859	0.0	0.11207525550497545	1.3537859848681094E-4
62-63	1.3537859848681094E-4	0.0012887001202109886	0.0	0.11892228846690454	1.4839577141823504E-4
64-65	1.3537859848681094E-4	0.001431889022456654	0.0	0.1251731349085744	1.536026405908047E-4
66-67	1.3537859848681094E-4	0.001444906195388078	0.0	0.13136149892017343	1.5620607517708953E-4
68-69	1.3537859848681094E-4	0.0014865611487686354	0.0	0.13724265765059085	1.5880950976337435E-4
70-71	1.3798203307309576E-4	0.001491768017941205	0.0	0.14206421850439035	1.6661981352222881E-4
72-73	1.5620607517708953E-4	0.0015047851908726291	0.0	0.14576630248608735	1.8744729021250744E-4
74-75	1.5620607517708953E-4	0.0015438367096669015	0.0	0.14882273469038576	1.8744729021250744E-4
76-77	1.5880950976337435E-4	0.0016167328780828767	0.0	0.15149646201050027	1.8744729021250744E-4
78-79	1.6141294434965917E-4	0.0016427672239457249	0.0	0.15356098563742415	1.8744729021250744E-4
80-81	1.6141294434965917E-4	0.0016505775277045793	0.0	0.15427953358323876	2.0046446314393154E-4
82-83	1.6141294434965917E-4	0.0016661981352222883	0.0	0.1547898067621506	2.1348163607535567E-4
84-85	1.7182668269479848E-4	0.001697439350257706	0.0	0.1549746506177768	2.1348163607535567E-4
86-87	1.7182668269479848E-4	0.001744301172810833	0.0	0.1551100292162636	2.1348163607535567E-4
88	1.8744729021250744E-4	0.0017859561261913902	0.0	0.15511783952002248	2.1348163607535567E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22555	0.0	49.293068	1
GTATCAA	40545	0.0	35.763126	1
ATCAACG	49130	0.0	28.762257	3
TCAACGC	49270	0.0	28.689056	4
CAACGCA	50120	0.0	28.185745	5
AACGCAG	51640	0.0	27.46186	6
TATCAAC	52085	0.0	27.346756	2
ACGCAGA	59265	0.0	23.836493	7
CGCAGAG	59910	0.0	23.558828	8
GTACATG	53370	0.0	19.41327	1
GCAGAGT	71980	0.0	19.386845	9
TACATGG	52880	0.0	19.13177	2
ACATGGG	53390	0.0	18.520823	3
GAGTACT	41860	0.0	18.469776	12-13
AGAGTAC	64405	0.0	18.36697	10-11
CAGAGTA	70250	0.0	17.589237	10-11
CATGGGG	37430	0.0	16.912476	4
GTACTTT	48730	0.0	15.923	14-15
AGTACTT	47355	0.0	15.718928	12-13
GTGGTAT	10325	0.0	15.441185	1
>>END_MODULE