FastQCFastQC Report
Fri 27 May 2016
SRR2139254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23262958
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273500.048.3826561
GTATCAA490300.035.584531
TCAACGC598700.028.2894764
ATCAACG598600.028.2871253
CAACGCA613450.027.5815455
TATCAAC639400.026.8224952
AACGCAG633000.026.78996
ACGCAGA721850.023.3927577
CGCAGAG730100.023.1284248
GTACATG645800.019.9322811
TACATGG632700.019.7820742
GCAGAGT864950.019.3318359
ACATGGG634650.019.0878813
AGAGTAC790150.018.50172810-11
GAGTACT523750.018.22700312-13
CAGAGTA851550.017.38647510-11
CATGGGG454350.017.2966354
AGTACTT584900.016.2019612-13
GTACTTT614100.015.63966914-15
GTGGTAT130450.014.2683481
Produced by FastQC (version 0.11.5)