FastQCFastQC Report
Fri 27 May 2016
SRR2139253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9233943
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196950.059.1103971
GTATCAA371350.039.3501971
TGGTATC73650.031.8366282
GTGGTAT79000.031.156431
ATCAACG468000.030.8269523
TCAACGC469450.030.6969364
TATCAAC474850.030.509572
CAACGCA478850.030.0766355
AACGCAG491700.029.404796
ATCTCGT91200.026.8989194
ACGCAGA557050.025.932077
CGCAGAG563750.025.647278
GCAGAGT629650.022.8907729
GTACATG471500.022.602861
TACATGG470250.022.2313562
GAGTACT372500.021.7566912-13
ACATGGG471800.021.5084883
TATCTCG99800.021.27323794
CAGAGTA625550.020.65457510-11
GTACTTT401200.020.19015114-15
Produced by FastQC (version 0.11.5)