##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9233943
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64831838359843	33.0	33.0	33.0	33.0	33.0
2	32.64945722536949	33.0	33.0	33.0	33.0	33.0
3	32.64347787288702	33.0	33.0	33.0	33.0	33.0
4	36.57203970178287	37.0	37.0	37.0	37.0	37.0
5	36.60362025193355	37.0	37.0	37.0	37.0	37.0
6	36.609931098773295	37.0	37.0	37.0	37.0	37.0
7	36.58621858506166	37.0	37.0	37.0	37.0	37.0
8	36.57933376890024	37.0	37.0	37.0	37.0	37.0
9	36.60561636561976	37.0	37.0	37.0	37.0	37.0
10-11	36.56012014585752	37.0	37.0	37.0	37.0	37.0
12-13	36.54895416833307	37.0	37.0	37.0	37.0	37.0
14-15	38.63852484252935	40.0	37.0	40.0	37.0	40.0
16-17	38.57927041568266	40.0	37.0	40.0	37.0	40.0
18-19	38.64065594730225	40.0	37.0	40.0	37.0	40.0
20-21	38.656024479665824	40.0	37.0	40.0	37.0	40.0
22-23	38.648556669320534	40.0	37.0	40.0	37.0	40.0
24-25	38.64599357055561	40.0	37.0	40.0	37.0	40.0
26-27	38.53198341969661	40.0	37.0	40.0	37.0	40.0
28-29	38.4800051122112	40.0	37.0	40.0	37.0	40.0
30-31	38.30471567988397	40.0	37.0	40.0	37.0	40.0
32-33	38.30646895692265	40.0	37.0	40.0	37.0	40.0
34-35	38.282962479072296	40.0	37.0	40.0	37.0	40.0
36-37	38.23817323587993	40.0	37.0	40.0	37.0	40.0
38-39	38.190871687901435	40.0	37.0	40.0	37.0	40.0
40-41	38.16570651108679	40.0	37.0	40.0	37.0	40.0
42-43	38.118390067339284	40.0	37.0	40.0	37.0	40.0
44-45	38.046418133825554	40.0	37.0	40.0	33.0	40.0
46-47	37.897217998725324	40.0	37.0	40.0	33.0	40.0
48-49	37.915655711323474	40.0	37.0	40.0	33.0	40.0
50-51	37.89805472449001	40.0	37.0	40.0	33.0	40.0
52-53	37.85986541029973	40.0	37.0	40.0	33.0	40.0
54-55	37.72244366113303	40.0	37.0	40.0	33.0	40.0
56-57	37.62567227168133	40.0	37.0	40.0	33.0	40.0
58-59	37.52398727285225	40.0	37.0	40.0	33.0	40.0
60-61	37.37656509904067	40.0	37.0	40.0	33.0	40.0
62-63	37.22615129049552	37.0	37.0	40.0	33.0	40.0
64-65	37.0659525981596	37.0	37.0	40.0	33.0	40.0
66-67	36.871560855200414	37.0	37.0	40.0	33.0	40.0
68-69	36.6579765751724	37.0	37.0	40.0	33.0	40.0
70-71	36.41894316261114	37.0	37.0	40.0	33.0	40.0
72-73	36.187503329672936	37.0	37.0	37.0	33.0	40.0
74-75	35.97452956607692	37.0	37.0	37.0	33.0	40.0
76-77	34.30818712528156	35.0	33.0	37.0	30.0	37.0
78-79	35.42610660450944	37.0	33.0	37.0	33.0	37.0
80-81	35.451615486447494	37.0	33.0	37.0	33.0	37.0
82-83	35.29250547580301	37.0	33.0	37.0	33.0	37.0
84-85	35.158013743995	37.0	33.0	37.0	33.0	37.0
86-87	35.0644151705128	37.0	33.0	37.0	33.0	37.0
88-89	34.924422554127545	37.0	33.0	37.0	33.0	37.0
90-91	34.785521548868346	37.0	33.0	37.0	33.0	37.0
92-93	34.79396881395928	37.0	33.0	37.0	33.0	37.0
94-95	34.832231871285536	37.0	33.0	37.0	33.0	37.0
96-97	34.8298919308063	37.0	33.0	37.0	33.0	37.0
98-99	34.840411082747615	37.0	33.0	37.0	33.0	37.0
100	34.8266545073866	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	5.0
19	7.0
20	58.0
21	305.0
22	1071.0
23	3069.0
24	7242.0
25	14230.0
26	24746.0
27	40270.0
28	59659.0
29	84873.0
30	115927.0
31	155717.0
32	207632.0
33	279600.0
34	387147.0
35	559001.0
36	989550.0
37	2600502.0
38	3601534.0
39	101793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.234796121223624	20.28600349818057	14.57880994067215	22.900390439923658
2	14.546743465927827	21.36866125337789	42.5613846652508	21.523210615443478
3	18.00091250292535	27.438516785299626	30.66845875050344	23.892111961271585
4	11.822435984281038	18.73362224566472	39.71451848901385	29.729423281040397
5	12.614621944276674	38.379270913844714	35.54893072222776	13.457176419650846
6	28.200035781089046	38.66001554268129	19.50871828327013	13.631230392959528
7	25.724882642225538	33.59928689185108	23.236953054616	17.43887741130739
8	24.113534164116025	36.56053540724694	21.460821233139516	17.865109195497524
9	26.1906886571304	16.58081673027619	21.298541836195202	35.9299527763982
10-11	23.79300634924933	27.36092165649072	28.79619857311113	20.049873421148824
12-13	25.167375726538417	25.212800537322067	28.872743981835274	20.74707975430424
14-15	22.278288370096213	26.216717147679887	27.380429366861254	24.124565115362646
16-17	20.823893974654165	29.338257773521022	28.06714856264545	21.770699689179366
18-19	20.692904428801434	27.902305656424346	30.66064518700191	20.74414472777231
20-21	22.13997035640542	26.874525244382045	30.66647295590564	20.319031443306894
22-23	21.59327480656713	27.129594675437254	30.373437795052922	20.903692722942697
24-25	21.074779113816444	27.332251914919247	30.70174825699411	20.8912207142702
26-27	21.002148398166614	27.566966843540776	30.544648978027695	20.886235780264915
28-29	20.850571712161823	27.482001332961577	30.691261339821512	20.976165615055095
30-31	21.241746113273653	27.35000342614934	30.561246664794172	20.847003795782836
32-33	20.878720274527808	27.542706254671344	30.49881257615515	21.079760894645705
34-35	21.174454226452035	27.631369028428608	30.413655440752187	20.780521304367173
36-37	21.087491227644563	27.582350862308058	30.30562219740865	21.024535712638727
38-39	20.898964363739893	27.78482876037584	30.285159283753238	21.031047592131028
40-41	21.2735256188185	27.555198627362724	30.242082770384627	20.929192983434145
42-43	21.109641363466082	27.66293826760546	29.88023201243413	21.347188356494332
44-45	21.23519106148	27.953472418103033	29.49933564374067	21.3120008766763
46-47	21.440465544408692	27.919424450028675	28.969699893220856	21.67041011234178
48-49	21.37071614498621	28.19128849055572	28.767312934888533	21.67068242956953
50-51	21.43918005714761	28.33334377298459	28.433001258562214	21.794474911305585
52-53	21.404135905386656	28.293705325031098	28.450010948429238	21.85214782115301
54-55	21.624812790610175	28.15050704457505	28.47239680842739	21.75228335638738
56-57	21.563108258582446	28.090088972508802	28.347220791266864	21.999581977641885
58-59	21.305686200504443	27.978652607614375	28.605475621118657	22.110185570762518
60-61	21.594775588345456	28.28563466655548	28.50925738733401	21.610332357765053
62-63	21.134214185796925	28.40215932496727	28.64789566700024	21.815730822235565
64-65	21.070115592024965	28.494839095056385	28.436541148288004	21.99850416463065
66-67	21.13425065792246	28.88210762155764	28.03822270914656	21.945419011373342
68-69	21.261380920005887	28.897132023868423	27.85637497078562	21.985112085340074
70-71	21.249474428362298	28.42214548408595	27.915784816634982	22.412595270916775
72-73	21.37508563274802	28.140982713809954	27.916888671792115	22.56704298164991
74-75	21.504101566215315	28.195263620073092	27.876010472020468	22.424624341691132
76-77	21.66436813582883	28.021663200721086	27.64551714935396	22.668451514096123
78-79	21.856665136030603	27.9678780711754	27.301439594598477	22.874017198195517
80-81	21.721509112192543	28.353434524654936	27.307127009755938	22.617929353396583
82-83	21.839594320781288	28.13693151298584	27.398130414223765	22.62534375200911
84-85	21.860496779557373	28.306610590523356	27.323360508933654	22.509532120985618
86-87	22.054506920790665	28.296368005369786	27.521664846147043	22.127460227692506
88-89	22.145670921907232	28.25839595153089	27.618060842533204	21.97787228402867
90-91	22.097286753546584	28.0139771716076	27.58310093446471	22.305635140381106
92-93	22.03189642931488	27.626847482488465	27.853067155238847	22.488188932957804
94-95	21.550330943101244	27.899195851684368	28.263605187857717	22.286868017356678
96-97	21.66526553796103	27.95509598248721	28.267916064081472	22.111722415470286
98-99	22.752552799585114	29.68147573380142	28.781882947729976	18.78408851888349
100	21.748514227007906	30.845362567623802	22.40412375156055	25.001999453807738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2319.0
1	2175.5
2	3335.0
3	5024.5
4	7346.0
5	12564.0
6	16599.0
7	18789.0
8	19838.0
9	19243.0
10	17965.5
11	16861.0
12	19086.5
13	26766.0
14	44341.5
15	68294.5
16	88139.5
17	99775.0
18	101153.0
19	92088.5
20	76899.5
21	60398.0
22	47107.0
23	40819.0
24	41689.5
25	48648.0
26	60233.5
27	74766.5
28	91142.5
29	111238.5
30	134301.5
31	158107.5
32	187134.0
33	218415.0
34	247870.0
35	276002.0
36	305031.5
37	329994.5
38	346639.0
39	359627.5
40	369403.5
41	374532.5
42	375322.5
43	380472.0
44	386436.0
45	388384.0
46	385124.5
47	378095.0
48	377697.5
49	371683.5
50	356501.5
51	338460.0
52	314799.0
53	288205.0
54	260269.0
55	232383.5
56	205245.5
57	178994.0
58	153978.0
59	129498.5
60	105212.5
61	82803.5
62	63067.5
63	46658.5
64	34296.0
65	25379.5
66	18613.5
67	13785.5
68	10291.5
69	7351.5
70	5296.5
71	3890.5
72	2731.5
73	1948.0
74	1358.0
75	897.5
76	567.0
77	340.5
78	227.5
79	153.5
80	116.0
81	87.5
82	60.5
83	45.0
84	35.0
85	27.0
86	22.5
87	20.5
88	18.0
89	13.0
90	9.5
91	7.5
92	5.0
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.6244414764093735E-4
7	0.0
8	0.0
9	1.0829609842729158E-5
10-11	0.006151218390670161
12-13	2.707402460682289E-5
14-15	0.006535669540087046
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	148.0
20-21	567.0
22-23	1145.0
24-25	2730.0
26-27	3538.0
28-29	6626.0
30-31	14442.0
32-33	19098.0
34-35	26822.0
36-37	35685.0
38-39	33542.0
40-41	53388.0
42-43	71629.0
44-45	90747.0
46-47	85839.0
48-49	72055.0
50-51	40841.0
52-53	19436.0
54-55	17333.0
56-57	17941.0
58-59	41223.0
60-61	67941.0
62-63	66786.0
64-65	70719.0
66-67	79085.0
68-69	79008.0
70-71	78526.0
72-73	75075.0
74-75	77056.0
76-77	77326.0
78-79	84772.0
80-81	86635.0
82-83	84316.0
84-85	84302.0
86-87	89103.0
88-89	96901.0
90-91	102004.0
92-93	104916.0
94-95	118410.0
96-97	302610.0
98-99	601997.0
100-101	6151680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.88595334783176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70741679559416	74.41306226921878
2	7.895458750710856	13.246361688437219
3	1.9337615663703658	4.866462976271237
4	0.7043277984086725	2.3633283535556386
5	0.29970872042515484	1.2570675869761445
6	0.15300005978652223	0.7700733526480604
7	0.09102640572052792	0.5345085776585107
8	0.05805260449129575	0.3895838457661571
9	0.03586832396480877	0.27079636956991776
>10	0.11822909388706061	1.4909666631214469
>50	0.0019038042769190364	0.10898470029276264
>100	0.0010617118715439793	0.16220965953875288
>500	1.433951716993385E-4	0.08076488908455658
>1k	4.096932044181176E-5	0.045829067860711066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.0829609842729158E-5	0.0	0.0	0.0
5	0.0	2.1659219685458315E-5	0.0	0.0	1.0829609842729158E-5
6	0.0	2.1659219685458315E-5	0.0	0.0	3.248882952818747E-5
7	0.0	2.1659219685458315E-5	0.0	0.0	3.248882952818747E-5
8	0.0	3.248882952818747E-5	0.0	2.1659219685458315E-5	5.414804921364578E-5
9	2.1659219685458315E-5	4.331843937091663E-5	0.0	2.1659219685458315E-5	7.58072688991041E-5
10-11	2.1659219685458315E-5	4.331843937091663E-5	0.0	2.1659219685458315E-5	7.58072688991041E-5
12-13	2.1659219685458315E-5	4.873324429228121E-5	0.0	2.1659219685458315E-5	7.58072688991041E-5
14-15	2.1659219685458315E-5	9.746648858456241E-5	0.0	5.414804921364578E-5	1.1912570827002073E-4
16-17	2.1659219685458315E-5	9.746648858456241E-5	0.0	7.58072688991041E-5	1.2995531811274989E-4
18-19	2.1659219685458315E-5	1.0288129350592698E-4	0.0	1.1912570827002073E-4	1.4078492795547903E-4
20-21	2.1659219685458315E-5	1.0829609842729157E-4	0.0	1.732737574836665E-4	1.4619973287684362E-4
22-23	2.1659219685458315E-5	1.0829609842729157E-4	0.0	2.9239946575368724E-4	1.6244414764093735E-4
24-25	2.1659219685458315E-5	1.1912570827002073E-4	0.0	7.093394446987598E-4	1.6244414764093735E-4
26-27	2.1659219685458315E-5	1.2995531811274989E-4	0.0	0.0020846998947253626	1.6244414764093735E-4
28-29	2.1659219685458315E-5	1.2995531811274989E-4	0.0	0.00777024506215817	1.6244414764093735E-4
30-31	2.1659219685458315E-5	1.4078492795547903E-4	0.0	0.01144689760376472	1.6244414764093735E-4
32-33	2.1659219685458315E-5	1.516145377982082E-4	0.0	0.015123550145371267	1.9493297716912481E-4
34-35	2.1659219685458315E-5	1.6244414764093738E-4	0.0	0.019872334061408002	1.9493297716912481E-4
36-37	2.1659219685458315E-5	1.8410336732639567E-4	0.0	0.029640642139549703	1.9493297716912481E-4
38-39	2.1659219685458315E-5	2.274218066973123E-4	0.0	0.11008298405134187	2.1117739193321857E-4
40-41	2.1659219685458315E-5	2.4366622146140604E-4	0.0	0.19657366306029828	2.1659219685458313E-4
42-43	2.1659219685458315E-5	2.761550509895935E-4	0.0	0.21915881438730994	2.2200700177594772E-4
44-45	2.1659219685458315E-5	3.0864388051778097E-4	0.0	0.2495900180453789	2.274218066973123E-4
46-47	2.1659219685458315E-5	3.411327100459685E-4	0.0	0.2819326478406895	2.490810263827706E-4
48-49	2.1659219685458315E-5	3.519623198886976E-4	0.0	0.3134738865076382	2.5991063622549977E-4
50-51	2.1659219685458315E-5	3.952807592596142E-4	0.0	0.34358020187042526	2.5991063622549977E-4
52-53	2.1659219685458315E-5	4.006955641809788E-4	0.0	0.6521428603143857	2.869846608323227E-4
54-55	2.1659219685458315E-5	4.006955641809788E-4	0.0	1.0830476211516575	3.3030310020323926E-4
56-57	2.1659219685458315E-5	4.006955641809788E-4	0.0	1.5762876162436783	3.3571790512460387E-4
58-59	2.1659219685458315E-5	4.061103691023434E-4	0.0	2.115889171072423	3.411327100459685E-4
60-61	2.1659219685458315E-5	4.11525174023708E-4	0.0	2.270844643507113	3.4654751496733305E-4
62-63	2.1659219685458315E-5	4.11525174023708E-4	0.0	2.2770121063125472	3.5737712481006216E-4
64-65	2.1659219685458315E-5	4.2235478386643715E-4	0.0	2.282535207332339	3.5737712481006216E-4
66-67	2.1659219685458315E-5	4.548436133946246E-4	0.0	2.2868670512694305	3.627919297314267E-4
68-69	2.1659219685458315E-5	4.6025841831598917E-4	0.0	2.291577931551018	3.7362153957415595E-4
70-71	2.1659219685458315E-5	4.6567322323735373E-4	0.0	2.2960830492455933	4.006955641809788E-4
72-73	2.1659219685458315E-5	4.6567322323735373E-4	0.0	2.299597257639559	4.4401400355189544E-4
74-75	2.1659219685458315E-5	4.8191763800144746E-4	0.0	2.302450859833118	4.4401400355189544E-4
76-77	2.1659219685458315E-5	4.873324429228121E-4	0.0	2.305201580733171	4.4401400355189544E-4
78-79	2.1659219685458315E-5	4.873324429228121E-4	0.0	2.306923488698165	4.4401400355189544E-4
80-81	2.1659219685458315E-5	4.873324429228121E-4	0.0	2.307546191264122	4.7650283308008296E-4
82-83	2.1659219685458315E-5	4.873324429228121E-4	0.0	2.3079902052676737	5.360656872150933E-4
84-85	2.1659219685458315E-5	4.873324429228121E-4	0.0	2.3082067974645284	5.414804921364579E-4
86-87	2.1659219685458315E-5	4.981620527655412E-4	0.0	2.3082988491481915	5.414804921364579E-4
88	2.1659219685458315E-5	5.306508822937287E-4	0.0	2.3082988491481915	5.414804921364579E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19695	0.0	59.110397	1
GTATCAA	37135	0.0	39.350197	1
TGGTATC	7365	0.0	31.836628	2
GTGGTAT	7900	0.0	31.15643	1
ATCAACG	46800	0.0	30.826952	3
TCAACGC	46945	0.0	30.696936	4
TATCAAC	47485	0.0	30.50957	2
CAACGCA	47885	0.0	30.076635	5
AACGCAG	49170	0.0	29.40479	6
ATCTCGT	9120	0.0	26.89891	94
ACGCAGA	55705	0.0	25.93207	7
CGCAGAG	56375	0.0	25.64727	8
GCAGAGT	62965	0.0	22.890772	9
GTACATG	47150	0.0	22.60286	1
TACATGG	47025	0.0	22.231356	2
GAGTACT	37250	0.0	21.75669	12-13
ACATGGG	47180	0.0	21.508488	3
TATCTCG	9980	0.0	21.273237	94
CAGAGTA	62555	0.0	20.654575	10-11
GTACTTT	40120	0.0	20.190151	14-15
>>END_MODULE