FastQCFastQC Report
Fri 27 May 2016
SRR2139252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9772196
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125850.052.085911
GTATCAA228600.037.684471
TCAACGC280900.030.0900334
ATCAACG283750.029.7727473
CAACGCA288800.029.2977265
TATCAAC293750.029.0355992
AACGCAG297100.028.4936266
ACGCAGA342550.024.6632797
CGCAGAG345800.024.3944078
TACATGG297300.022.7671342
GTACATG307350.022.439771
ACATGGG301800.021.719723
GCAGAGT407500.020.586639
GAGTACT251700.019.43154512-13
GTGGTAT59500.019.175311
CATGGGG207250.019.1544324
TGGTATC54950.018.507912
AGAGTAC371550.018.47229410-11
CAGAGTA400500.018.43884710-11
GTACTTT280050.017.36588914-15
Produced by FastQC (version 0.11.5)