##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9772196
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.296809130721485	33.0	33.0	33.0	33.0	33.0
2	32.337712219443816	33.0	33.0	33.0	33.0	33.0
3	32.363199325924285	33.0	33.0	33.0	33.0	33.0
4	36.224510130578636	37.0	37.0	37.0	33.0	37.0
5	36.276846882727284	37.0	37.0	37.0	33.0	37.0
6	36.26027680983885	37.0	37.0	37.0	33.0	37.0
7	36.228793507621006	37.0	37.0	37.0	33.0	37.0
8	36.213217786462735	37.0	37.0	37.0	33.0	37.0
9	36.27935604238802	37.0	37.0	37.0	33.0	37.0
10-11	36.19827135067696	37.0	37.0	37.0	33.0	37.0
12-13	36.15834332426407	37.0	37.0	37.0	33.0	37.0
14-15	37.89501530669258	40.0	37.0	40.0	33.0	40.0
16-17	37.82311043495239	40.0	37.0	40.0	33.0	40.0
18-19	37.79105182704072	40.0	37.0	40.0	33.0	40.0
20-21	37.80177761279667	40.0	37.0	40.0	33.0	40.0
22-23	37.806413334889676	40.0	37.0	40.0	33.0	40.0
24-25	37.80254528192907	40.0	37.0	40.0	33.0	40.0
26-27	37.66622213180581	40.0	37.0	40.0	33.0	40.0
28-29	37.59437410866061	40.0	37.0	40.0	33.0	40.0
30-31	37.46702166594899	40.0	37.0	40.0	33.0	40.0
32-33	37.41480581838681	40.0	37.0	40.0	33.0	40.0
34-35	37.38467612436733	40.0	37.0	40.0	33.0	40.0
36-37	37.32752014119149	40.0	37.0	40.0	33.0	40.0
38-39	37.271301185101585	40.0	37.0	40.0	33.0	40.0
40-41	37.24783840967946	40.0	37.0	40.0	33.0	40.0
42-43	37.16086772135492	40.0	37.0	40.0	33.0	40.0
44-45	37.098245923823754	40.0	37.0	40.0	33.0	40.0
46-47	37.05000548638659	40.0	37.0	40.0	33.0	40.0
48-49	37.03945787947438	40.0	37.0	40.0	33.0	40.0
50-51	36.95674409932286	37.0	37.0	40.0	33.0	40.0
52-53	36.84243063733758	37.0	37.0	40.0	33.0	40.0
54-55	36.65080581463095	37.0	37.0	40.0	33.0	40.0
56-57	36.54479615797157	37.0	37.0	40.0	33.0	40.0
58-59	36.40742044289645	37.0	37.0	40.0	33.0	40.0
60-61	36.264626913654396	37.0	37.0	40.0	33.0	40.0
62-63	36.09504639060235	37.0	37.0	40.0	33.0	40.0
64-65	35.9212512799971	37.0	33.0	40.0	33.0	40.0
66-67	35.72396995702276	37.0	33.0	40.0	30.0	40.0
68-69	35.50811665279754	37.0	33.0	38.5	27.0	40.0
70-71	35.274628040324245	37.0	33.0	37.0	27.0	40.0
72-73	35.05707696989627	37.0	33.0	37.0	27.0	40.0
74-75	34.85914707518487	37.0	33.0	37.0	27.0	40.0
76-77	32.900934065103925	33.0	33.0	37.0	27.0	37.0
78-79	34.31605330132922	37.0	33.0	37.0	27.0	37.0
80-81	34.24522838412051	37.0	33.0	37.0	27.0	37.0
82-83	33.91604755987421	37.0	33.0	37.0	27.0	37.0
84-85	34.01988121695162	37.0	33.0	37.0	27.0	37.0
86-87	33.95982696695215	37.0	33.0	37.0	27.0	37.0
88-89	33.837855508617935	37.0	33.0	37.0	27.0	37.0
90-91	33.49204167681937	37.0	33.0	37.0	27.0	37.0
92-93	33.64354742919085	33.0	33.0	37.0	27.0	37.0
94-95	33.547483703931306	33.0	33.0	37.0	27.0	37.0
96-97	33.5810327476662	33.0	33.0	37.0	27.0	37.0
98-99	33.73852328668697	33.0	33.0	37.0	27.0	37.0
100	33.78185425712763	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	16.0
20	131.0
21	635.0
22	2470.0
23	7101.0
24	16061.0
25	31342.0
26	53912.0
27	85303.0
28	123629.0
29	171247.0
30	227770.0
31	296362.0
32	385111.0
33	500415.0
34	658527.0
35	900961.0
36	1418286.0
37	2615962.0
38	2226155.0
39	50797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5947346942284	20.39150667874447	14.030531110919183	23.983227516107945
2	14.581502458608075	21.48408607440948	41.62321345171546	22.31119801526699
3	17.89591612775675	26.6985946659277	30.816492014691477	24.588997191624074
4	12.105160395882358	18.453027344109756	39.26003940158384	30.181772858424043
5	13.00162215330106	38.12521770950972	35.88289674091678	12.990263396272445
6	29.2915635339283	38.57446166654864	18.941801822231156	13.1921729772919
7	25.741972428715105	33.04721886462367	23.59080804355541	17.620000663105817
8	24.01843966289665	37.173149208223	21.448321339441	17.360089789439346
9	25.530241887412963	16.523500205839202	21.207833534976956	36.738424371770876
10-11	23.81331577960382	26.935307633288403	29.345012396511322	19.906364190596456
12-13	24.734900069217012	24.61018357858096	29.312422509487153	21.34249384271488
14-15	21.88592643365606	25.888797117690782	26.954891107520996	25.27038534113217
16-17	21.391348474795223	29.00734901346637	27.646206645875708	21.9550958658627
18-19	21.466577215602307	28.027113864683024	29.40910108638836	21.097207833326305
20-21	22.21885483105023	27.269815375828742	29.3319737782847	21.17935601483633
22-23	21.90209618870176	27.32464353370288	29.16805878164894	21.605201495946417
24-25	21.746403133522175	27.442743698046097	29.272842751508616	21.538010416923118
26-27	21.700749928009696	27.63443037134708	29.170302023388352	21.494517677254873
28-29	21.638280568181713	27.620843849628567	29.212528746361972	21.52834683582775
30-31	21.83162797211335	27.528441567535307	29.184373687891835	21.45555677245951
32-33	21.701489351463994	27.70284512069081	29.07231495796149	21.523350569883704
34-35	21.906675714409594	27.629160018943754	29.039718058527047	21.424446208119605
36-37	21.721121756791405	27.66133164504256	28.97409988235901	21.643446715807027
38-39	21.578776482047672	27.693681355847538	29.070867510151583	21.656674651953203
40-41	21.83088127655069	27.590557258892513	28.973989267485557	21.604572197071246
42-43	21.80847720959277	27.653125670174784	28.770224561330682	21.768172558901764
44-45	21.815066518469106	27.875657616060217	28.52459536894301	21.784680496527667
46-47	21.970635263825777	27.76950531354342	28.198079435941985	22.06177998668882
48-49	21.863937195963697	27.92448152671802	28.191485567819374	22.02009570949891
50-51	21.83530385035379	28.045660283798668	28.09432670037176	22.02470916547578
52-53	21.977239610807892	27.998292274697118	28.03968087965941	21.98478723483558
54-55	22.01213220123584	27.884250235160916	27.988455390709195	22.11516217289405
56-57	21.996845281054203	28.02361226323352	27.86144134709233	22.118101108619946
58-59	21.916604471906844	28.07525224301579	27.974463688509378	22.033679596567993
60-61	22.069444189627262	28.107760853212604	27.851289006434094	21.97150595072604
62-63	21.9392997968694	28.213082293498115	27.837393100387487	22.010224809245003
64-65	21.92513368983957	28.155834648574	27.78961542190683	22.129416239679593
66-67	22.01464178480272	28.20118837716282	27.61607159811464	22.16809823991982
68-69	21.991263670602397	28.144888662932544	27.70588664977838	22.15796101668668
70-71	22.021580289671803	27.942889026775415	27.749587716669932	22.285942966882853
72-73	22.053552428133703	27.98498162883551	27.650839240033516	22.310626702997276
74-75	21.97036196831013	28.097037602365237	27.65679304902059	22.275807380304045
76-77	22.184947063224612	27.992288971373764	27.526262242879817	22.296501722521807
78-79	22.07569522174456	28.068553387102014	27.548445640836356	22.30730575031707
80-81	22.116164587004857	28.0410495691171	27.51737972818582	22.32540611569222
82-83	22.161427687074404	28.042082752324553	27.5579497633236	22.23853979727744
84-85	22.187483787121213	28.078946142815138	27.57593219827015	22.1576378717935
86-87	22.067368659167062	28.068583412703408	27.655784472944895	22.20826345518464
88-89	22.09937157847896	28.09711686725792	27.62493402738252	22.17857752688061
90-91	22.213105936635742	28.127151993500256	27.616117758985382	22.043624310878624
92-93	22.171627072587516	28.087509328252143	27.668644791395913	22.07221880776443
94-95	21.996943010448046	28.116599226182625	27.714889496369892	22.171568266999437
96-97	22.050394576051232	28.223736711161646	27.857659029232767	21.86820968355436
98-99	23.089503832195255	30.173809570548787	28.071436635294734	18.665249961961223
100	22.048494022314337	31.182130597212854	21.980608832273692	24.788766548199117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3784.0
1	3142.5
2	3661.5
3	5084.0
4	6659.5
5	10576.0
6	13999.5
7	16422.0
8	17940.5
9	17372.5
10	15526.0
11	13783.0
12	14816.5
13	19283.0
14	29524.5
15	44062.5
16	55849.5
17	62203.5
18	63593.5
19	60944.5
20	56182.0
21	51601.5
22	49334.0
23	51659.5
24	59839.5
25	73863.0
26	92222.5
27	111524.5
28	129414.5
29	147959.5
30	166754.5
31	186106.0
32	207809.5
33	229723.5
34	251242.5
35	272319.5
36	294347.5
37	314274.0
38	328885.5
39	341206.0
40	354823.5
41	365330.5
42	372340.0
43	380514.0
44	386347.5
45	390857.0
46	397145.0
47	402238.0
48	402504.5
49	398533.0
50	392520.5
51	381310.0
52	363323.5
53	341480.0
54	317674.5
55	292591.0
56	267465.0
57	241272.5
58	214038.0
59	184554.5
60	152303.5
61	122441.5
62	95880.5
63	73136.0
64	54330.5
65	39552.0
66	28949.5
67	21886.0
68	16655.5
69	11980.5
70	8488.5
71	5927.0
72	4028.0
73	2681.0
74	1810.5
75	1201.0
76	788.0
77	534.0
78	368.0
79	261.0
80	185.0
81	129.0
82	96.0
83	68.0
84	46.5
85	33.5
86	26.0
87	20.0
88	14.5
89	10.5
90	7.0
91	4.5
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.116557220096691E-5
10-11	0.0030034190881967574
12-13	3.2745966208618817E-4
14-15	0.003162032362019755
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	217.0
20-21	828.0
22-23	1654.0
24-25	3199.0
26-27	5203.0
28-29	9406.0
30-31	20022.0
32-33	25514.0
34-35	35677.0
36-37	48261.0
38-39	54467.0
40-41	59617.0
42-43	70760.0
44-45	82942.0
46-47	82165.0
48-49	73598.0
50-51	62223.0
52-53	58553.0
54-55	64037.0
56-57	69035.0
58-59	70021.0
60-61	69647.0
62-63	71812.0
64-65	75842.0
66-67	81700.0
68-69	78713.0
70-71	78632.0
72-73	74992.0
74-75	78847.0
76-77	74224.0
78-79	85713.0
80-81	87644.0
82-83	83756.0
84-85	86011.0
86-87	92022.0
88-89	101843.0
90-91	105906.0
92-93	115217.0
94-95	143482.0
96-97	349013.0
98-99	605557.0
100-101	6334224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.09740443099817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.44595355931621	76.11618484753411
2	7.439198092371148	12.661128974176354
3	1.7889731546929442	4.567109161833124
4	0.6375875707842207	2.1702818948481006
5	0.29255526149370265	1.2447846702873018
6	0.14162432962575863	0.7231117713259307
7	0.07720361915454743	0.45988793219118956
8	0.04431640608962879	0.3016968905549993
9	0.032236797814146075	0.24689410390356356
>10	0.09809321557246599	1.2873541931679002
>50	0.0017499206885596486	0.10035137812798628
>100	3.945448277045723E-4	0.0550054202774323
>500	9.460741273893575E-5	0.0478702218360648
>1k	1.8920156199665536E-5	0.01833853993599458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.069934332058014E-5	0.0	0.0	0.0
3	0.0	3.069934332058014E-5	0.0	1.0233114440193382E-5	0.0
4	0.0	7.163180108135367E-5	0.0	2.0466228880386764E-5	0.0
5	1.0233114440193382E-5	7.163180108135367E-5	0.0	2.0466228880386764E-5	0.0
6	4.093245776077353E-5	7.163180108135367E-5	0.0	2.0466228880386764E-5	0.0
7	4.093245776077353E-5	7.163180108135367E-5	0.0	2.0466228880386764E-5	1.0233114440193382E-5
8	6.139868664116028E-5	8.186491552154706E-5	0.0	2.0466228880386764E-5	3.069934332058014E-5
9	6.139868664116028E-5	1.3303048772251395E-4	0.0	7.163180108135367E-5	4.093245776077353E-5
10-11	6.651524386125698E-5	1.3303048772251395E-4	0.0	9.209802996174043E-5	4.093245776077353E-5
12-13	7.163180108135367E-5	1.3303048772251395E-4	0.0	1.2791393050241726E-4	4.093245776077353E-5
14-15	8.698147274164374E-5	2.2512851768425438E-4	0.0	1.5861327382299742E-4	9.209802996174043E-5
16-17	9.209802996174043E-5	2.3536163212444777E-4	0.0	1.7907950270338417E-4	1.1256425884212719E-4
18-19	9.209802996174043E-5	2.5071130378473785E-4	0.0	2.097788460239643E-4	1.1256425884212719E-4
20-21	9.209802996174043E-5	2.660609754450279E-4	0.0	2.762940898852213E-4	1.1768081606222387E-4
22-23	9.721458718183712E-5	3.0187687598570473E-4	0.0	3.325762193062849E-4	1.3814704494261065E-4
24-25	1.3303048772251395E-4	3.2745966208618817E-4	0.0	4.707232642488955E-4	1.5861327382299742E-4
26-27	1.3303048772251395E-4	3.4792589096657495E-4	0.0	8.135325979953738E-4	1.637298310430941E-4
28-29	1.8931261714357755E-4	3.4792589096657495E-4	0.0	0.0029573700732158873	1.637298310430941E-4
30-31	2.097788460239643E-4	4.0932457760773527E-4	0.0	0.009905654778107193	1.688463882631908E-4
32-33	2.3536163212444774E-4	4.0932457760773527E-4	0.0	0.02431899646711957	1.9442917436367425E-4
34-35	2.916437615455114E-4	4.5025703536850876E-4	0.0	0.04157202741328561	1.9442917436367425E-4
36-37	3.274596620861882E-4	4.963060503493789E-4	0.0	0.06713946384210877	1.9442917436367425E-4
38-39	3.8374179150725183E-4	5.065391647895724E-4	0.0	0.11234424688166303	1.9442917436367425E-4
40-41	3.990914631675419E-4	5.321219508900558E-4	0.0	0.15717040468693014	1.9442917436367425E-4
42-43	4.1444113482783194E-4	5.525881797704426E-4	0.0	0.19036662793091746	1.9442917436367425E-4
44-45	4.4514047814841204E-4	5.935206375312161E-4	0.0	0.23063393325307843	1.9442917436367425E-4
46-47	5.11655722009669E-4	6.242199808517962E-4	0.0	0.2721752613230435	1.9954573158377094E-4
48-49	5.218888364498625E-4	6.344530952919897E-4	0.0	0.3022708508916522	2.14895403244061E-4
50-51	5.218888364498625E-4	6.907352247130532E-4	0.0	0.3305295964182462	2.200119604641577E-4
52-53	5.218888364498625E-4	6.958517819331499E-4	0.0	0.3889555633145303	2.762940898852213E-4
54-55	5.372385081101525E-4	7.060848963733433E-4	0.0	0.45688297696853397	3.428093337464783E-4
56-57	6.037537519714095E-4	7.060848963733433E-4	0.0	0.5245545627615329	3.530424481866716E-4
58-59	6.037537519714095E-4	7.163180108135367E-4	0.0	0.5796036018925531	3.6327556262686505E-4
60-61	6.037537519714095E-4	7.265511252537301E-4	0.0	0.623989735776892	3.786252342871551E-4
62-63	6.139868664116029E-4	7.470173541341169E-4	0.0	0.6592735143666787	4.1955769204792866E-4
64-65	6.139868664116029E-4	8.953975135169209E-4	0.0	0.6647942796071631	4.1955769204792866E-4
66-67	6.242199808517962E-4	9.10747185177211E-4	0.0	0.6694247638913505	4.1955769204792866E-4
68-69	6.44686209732183E-4	9.312134140575976E-4	0.0	0.6742547939071217	4.1955769204792866E-4
70-71	6.549193241723765E-4	9.465630857178877E-4	0.0	0.6787317814747064	4.400239209283154E-4
72-73	6.600358813924731E-4	9.619127573781778E-4	0.0	0.6821547582549511	4.5025703536850876E-4
74-75	6.651524386125698E-4	9.926121006987578E-4	0.0	0.6846925706361191	4.5025703536850876E-4
76-77	6.651524386125698E-4	0.0010540107873399183	0.0	0.6878443698836986	4.707232642488955E-4
78-79	6.651524386125698E-4	0.0010847101306604985	0.0	0.6898347106423162	4.911894931292823E-4
80-81	7.572504685743102E-4	0.0010847101306604985	0.0	0.6905356789814694	5.065391647895724E-4
82-83	7.879498118948903E-4	0.0010847101306604985	0.0	0.6909603532307376	5.321219508900558E-4
84-85	8.032994835551804E-4	0.0010949432451006918	0.0	0.6912110645345222	5.321219508900558E-4
86-87	8.186491552154705E-4	0.0010949432451006918	0.0	0.6912622301067233	5.321219508900558E-4
88	8.186491552154705E-4	0.0010949432451006918	0.0	0.6912673466639433	5.423550653302492E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12585	0.0	52.08591	1
GTATCAA	22860	0.0	37.68447	1
TCAACGC	28090	0.0	30.090033	4
ATCAACG	28375	0.0	29.772747	3
CAACGCA	28880	0.0	29.297726	5
TATCAAC	29375	0.0	29.035599	2
AACGCAG	29710	0.0	28.493626	6
ACGCAGA	34255	0.0	24.663279	7
CGCAGAG	34580	0.0	24.394407	8
TACATGG	29730	0.0	22.767134	2
GTACATG	30735	0.0	22.43977	1
ACATGGG	30180	0.0	21.71972	3
GCAGAGT	40750	0.0	20.58663	9
GAGTACT	25170	0.0	19.431545	12-13
GTGGTAT	5950	0.0	19.17531	1
CATGGGG	20725	0.0	19.154432	4
TGGTATC	5495	0.0	18.50791	2
AGAGTAC	37155	0.0	18.472294	10-11
CAGAGTA	40050	0.0	18.438847	10-11
GTACTTT	28005	0.0	17.365889	14-15
>>END_MODULE