FastQCFastQC Report
Fri 27 May 2016
SRR2139251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15180154
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208500.052.0971871
GTATCAA385250.037.2680741
TCAACGC476450.029.7033734
ATCAACG482500.029.2954623
CAACGCA488200.029.0080055
TATCAAC495700.028.78292
AACGCAG501050.028.2982186
ACGCAGA569100.024.9071947
CGCAGAG574100.024.6381868
GTACATG474400.021.2104681
GCAGAGT671750.020.8538329
TACATGG472400.020.7931162
GAGTACT404650.020.05730612-13
ACATGGG479650.020.0417773
GTGGTAT98400.019.5647851
AGAGTAC612950.018.91729710-11
CAGAGTA655400.018.76595910-11
TGGTATC96650.018.4583052
CATGGGG338950.018.1880444
GTACTTT460950.017.5799714-15
Produced by FastQC (version 0.11.5)