##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15180154
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.273183855710556	33.0	33.0	33.0	33.0	33.0
2	32.324970747991095	33.0	33.0	33.0	33.0	33.0
3	32.3461961584843	33.0	33.0	33.0	33.0	33.0
4	36.207069440797504	37.0	37.0	37.0	33.0	37.0
5	36.25663323310159	37.0	37.0	37.0	33.0	37.0
6	36.24282579741945	37.0	37.0	37.0	33.0	37.0
7	36.209953864763165	37.0	37.0	37.0	33.0	37.0
8	36.19590460017731	37.0	37.0	37.0	33.0	37.0
9	36.25999703296818	37.0	37.0	37.0	33.0	37.0
10-11	36.17996747595578	37.0	37.0	37.0	33.0	37.0
12-13	36.13931867884871	37.0	37.0	37.0	33.0	37.0
14-15	37.85863766599469	40.0	37.0	40.0	33.0	40.0
16-17	37.78935991031448	40.0	37.0	40.0	33.0	40.0
18-19	37.76132113020724	40.0	37.0	40.0	33.0	40.0
20-21	37.7722996164466	40.0	37.0	40.0	33.0	40.0
22-23	37.77739421940014	40.0	37.0	40.0	33.0	40.0
24-25	37.77157041514309	40.0	37.0	40.0	33.0	40.0
26-27	37.630059153602446	40.0	37.0	40.0	33.0	40.0
28-29	37.555957876743946	40.0	37.0	40.0	33.0	40.0
30-31	37.43275203729168	40.0	37.0	40.0	33.0	40.0
32-33	37.37752162801887	40.0	37.0	40.0	33.0	40.0
34-35	37.34692734312614	40.0	37.0	40.0	33.0	40.0
36-37	37.28927087690046	40.0	37.0	40.0	33.0	40.0
38-39	37.23084280131498	40.0	37.0	40.0	33.0	40.0
40-41	37.20615454336985	40.0	37.0	40.0	33.0	40.0
42-43	37.117766240042144	40.0	37.0	40.0	33.0	40.0
44-45	37.054450545747926	40.0	37.0	40.0	33.0	40.0
46-47	37.00119581648822	37.0	37.0	40.0	33.0	40.0
48-49	36.9928216101348	37.0	37.0	40.0	33.0	40.0
50-51	36.909041639530784	37.0	37.0	40.0	33.0	40.0
52-53	36.79726687002284	37.0	37.0	40.0	33.0	40.0
54-55	36.602750032241396	37.0	37.0	40.0	33.0	40.0
56-57	36.493711904749375	37.0	37.0	40.0	33.0	40.0
58-59	36.35469930924375	37.0	37.0	40.0	33.0	40.0
60-61	36.212206483643996	37.0	37.0	40.0	33.0	40.0
62-63	36.04021001323023	37.0	37.0	40.0	33.0	40.0
64-65	35.86919991383946	37.0	33.0	40.0	33.0	40.0
66-67	35.66816346796531	37.0	33.0	40.0	27.0	40.0
68-69	35.451214985236874	37.0	33.0	38.5	27.0	40.0
70-71	35.21845814813274	37.0	33.0	37.0	27.0	40.0
72-73	35.001664750755864	37.0	33.0	37.0	27.0	40.0
74-75	34.80137407080518	37.0	33.0	37.0	27.0	40.0
76-77	32.84174394122675	33.0	33.0	37.0	27.0	37.0
78-79	34.26106080510368	37.0	33.0	37.0	27.0	37.0
80-81	34.19233831145252	37.0	33.0	37.0	27.0	37.0
82-83	33.86569198705399	37.0	33.0	37.0	27.0	37.0
84-85	33.96749290030131	37.0	33.0	37.0	27.0	37.0
86-87	33.903354788578504	37.0	33.0	37.0	27.0	37.0
88-89	33.7822261810648	37.0	33.0	37.0	27.0	37.0
90-91	33.44676910597971	33.0	33.0	37.0	27.0	37.0
92-93	33.594112496040324	33.0	33.0	37.0	27.0	37.0
94-95	33.500223494876096	33.0	33.0	37.0	27.0	37.0
96-97	33.53719694088478	33.0	33.0	37.0	27.0	37.0
98-99	33.70021522232257	33.0	33.0	37.0	27.0	37.0
100	33.743348413120614	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	8.0
19	42.0
20	236.0
21	1202.0
22	4314.0
23	11763.0
24	27226.0
25	52158.0
26	89291.0
27	138801.0
28	199541.0
29	274387.0
30	362766.0
31	471573.0
32	605993.0
33	784439.0
34	1030402.0
35	1406032.0
36	2203136.0
37	4043831.0
38	3394619.0
39	78390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.357874235004466	20.499910606967493	14.39030196926856	23.75191318875948
2	14.526736685280003	21.467904739306334	41.71532778916472	22.29003078624894
3	17.842085132996672	26.834688238340664	30.82609702115012	24.497129607512548
4	12.222675738335724	18.62398760908486	39.32510829600279	29.82822835657662
5	13.129926086388846	38.03425182643075	35.74208140444425	13.093740682736158
6	29.153854433887826	38.53173031050937	19.07501070147246	13.239404554130346
7	25.74833562294559	33.04751717275069	23.614450815189358	17.589696389114366
8	24.06251609832153	36.98724663794584	21.38233248490101	17.567904778831625
9	25.47698468368433	16.70879843627389	21.324298992526558	36.48991788751522
10-11	23.880471995437595	26.924200131978694	29.224284922722237	19.97104294986147
12-13	24.9465558861746	24.604147288924366	29.19283289754926	21.256463927351778
14-15	22.00714120337851	25.860302024660708	27.001441304214136	25.131115467746646
16-17	21.452740202767377	28.929512177544442	27.604176479369052	22.01357114031913
18-19	21.448810730115124	27.988457824604414	29.467352570994997	21.095378874285466
20-21	22.2879080991065	27.159508706752188	29.361741780621642	21.190841413519664
22-23	22.01627727997977	27.15873724612911	29.207654869008724	21.617330604882394
24-25	21.800167920025782	27.359427938386265	29.248244846904782	21.59215929468317
26-27	21.731285303226375	27.536517396108028	29.176375491745954	21.555821808919642
28-29	21.62432026210018	27.591939432608843	29.208929291426017	21.574811013864963
30-31	22.000080049250105	27.38375868083591	29.08171753723859	21.5344437326754
32-33	21.739413605969478	27.56236675243468	29.042478085273217	21.655741556322628
34-35	21.846669159061115	27.512440789316056	29.068270347499208	21.57261970412362
36-37	21.80831983148305	27.614745073207146	28.941154452505803	21.635780642804004
38-39	21.684247404824305	27.611014747035007	29.03479020482073	21.669947643319954
40-41	21.943729642107872	27.47598488077306	28.886099383503822	21.694186093615247
42-43	21.89186113363348	27.545875704269314	28.683507629581417	21.878755532515786
44-45	21.942953358157528	27.72866000963451	28.42685285415782	21.901533778050144
46-47	22.064313159045454	27.712235864865598	28.099881280145222	22.12356969594373
48-49	21.964599216648345	27.864313898655134	28.041817909923978	22.129268974772543
50-51	21.952680772533547	28.004663236166007	27.9256890752901	22.116966916010348
52-53	22.071938228555442	27.895885326547397	27.865724534533946	22.16645191036321
54-55	22.107744004349364	27.83132354530091	27.875822123555555	22.18511032679417
56-57	22.113518860286614	27.91708910925453	27.76759138774863	22.20180064271023
58-59	22.047333517323626	27.967861509988573	27.852600177102648	22.132204795585153
60-61	22.160012931322143	28.010696688755743	27.770471061979755	22.05881931794236
62-63	22.04077260643044	28.05881405021795	27.760538140215395	22.13987520313621
64-65	22.033722031423984	28.003383730216385	27.688908211162	22.273986027197633
66-67	22.110285272287804	28.074655400319653	27.565663598192053	22.24939572920049
68-69	22.07820961604286	28.03858352356423	27.615365634572438	22.26784122582048
70-71	22.07589396369917	27.840515657538667	27.682114368470508	22.401476010291656
72-73	22.165148019890257	27.808203079631344	27.60966256476657	22.41698633571183
74-75	22.122442582755717	27.954789301921203	27.536553033485884	22.386215081837197
76-77	22.296207145853327	27.79565499245507	27.46565092231797	22.442486939373634
78-79	22.18558572559159	27.85891102041445	27.471081829150567	22.484421424843397
80-81	22.22212691288325	27.85960283089387	27.485207891311454	22.43306236491142
82-83	22.289390054368685	27.809831299407666	27.533091551086418	22.367687095137228
84-85	22.34001183943742	27.820965139411708	27.52360733899004	22.315415682160825
86-87	22.19379494115475	27.851848062277647	27.618457584689605	22.335899411878003
88-89	22.236242927406824	27.819193579892808	27.575681247858192	22.368882244842176
90-91	22.388588691489	27.748523155437578	27.58553482349108	22.277353329582336
92-93	22.29892670974528	27.728311291837983	27.664791621583866	22.307970376832873
94-95	22.19812569168632	27.749895803558516	27.71156056797443	22.340417936780728
96-97	22.185357350921212	27.868911752473558	27.85873810515106	22.086992791454172
98-99	23.291912472484334	29.752970455791072	28.060228192477926	18.894888879246672
100	22.22344714531504	30.814273903254417	21.87312253390916	25.08915641752138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3175.0
1	2891.0
2	4470.5
3	6754.5
4	9297.0
5	15576.0
6	20891.5
7	24365.5
8	26526.5
9	26109.0
10	23938.0
11	21194.0
12	22121.5
13	28640.0
14	44683.0
15	67424.0
16	86341.0
17	97776.0
18	101181.5
19	96650.5
20	87741.5
21	78139.0
22	71666.5
23	72873.0
24	83203.5
25	101739.5
26	127625.5
27	157052.5
28	187244.5
29	221141.0
30	255875.0
31	288612.0
32	323056.5
33	359145.0
34	395169.0
35	428638.5
36	464254.5
37	496692.5
38	517791.5
39	534673.5
40	553528.0
41	571514.5
42	584109.0
43	595481.5
44	604026.5
45	612974.0
46	620919.5
47	627199.5
48	628053.0
49	617284.0
50	604625.0
51	586439.0
52	559266.5
53	527841.0
54	493779.0
55	455461.5
56	415310.0
57	374342.5
58	330119.5
59	283526.5
60	236503.5
61	191708.0
62	150758.0
63	115525.5
64	86088.5
65	63638.5
66	47603.0
67	36004.5
68	27496.0
69	19835.5
70	13826.0
71	10050.0
72	7263.5
73	5082.5
74	3371.5
75	2201.5
76	1451.0
77	944.0
78	628.5
79	435.5
80	321.5
81	240.5
82	177.5
83	116.5
84	74.5
85	51.5
86	35.0
87	26.0
88	19.0
89	13.5
90	9.0
91	6.0
92	3.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.317509690613152E-5
10-11	0.0028392333832713426
12-13	2.931459061614263E-4
14-15	0.0032904804523063466
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	355.0
20-21	1370.0
22-23	2770.0
24-25	6487.0
26-27	7361.0
28-29	14093.0
30-31	30652.0
32-33	37944.0
34-35	53639.0
36-37	72253.0
38-39	82363.0
40-41	91854.0
42-43	109578.0
44-45	129446.0
46-47	124375.0
48-49	110994.0
50-51	94169.0
52-53	86777.0
54-55	95165.0
56-57	103328.0
58-59	104291.0
60-61	107918.0
62-63	108339.0
64-65	115060.0
66-67	123669.0
68-69	122288.0
70-71	122553.0
72-73	118813.0
74-75	124168.0
76-77	117731.0
78-79	135176.0
80-81	138403.0
82-83	133872.0
84-85	139042.0
86-87	148637.0
88-89	164590.0
90-91	172941.0
92-93	188810.0
94-95	224410.0
96-97	545923.0
98-99	944801.0
100-101	9823746.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.49745640398892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67634043896837	71.45398732586116
2	8.07354012102006	13.159459680773752
3	2.2450331437822446	5.48893472282711
4	0.8894025514753063	2.899361826578212
5	0.4321196955266067	1.76083280237423
6	0.2268012704856357	1.1090235992263437
7	0.1405027020036982	0.8015428981832329
8	0.08616716215825573	0.5617923633158313
9	0.054594023976107645	0.4004346680020043
>10	0.1722092940826072	2.0987077192623786
>50	0.0025336610794744817	0.1440041969782981
>100	7.1124471147131E-4	0.08867754916076512
>500	2.97946788733795E-5	0.01850280538229704
>1k	1.4896051235962798E-5	0.014737842074400787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.58754845306576E-6
2	0.0	1.9762645359197277E-5	0.0	0.0	6.58754845306576E-6
3	0.0	1.9762645359197277E-5	0.0	6.58754845306576E-6	6.58754845306576E-6
4	0.0	4.6112839171460316E-5	0.0	3.29377422653288E-5	6.58754845306576E-6
5	0.0	4.6112839171460316E-5	0.0	4.6112839171460316E-5	6.58754845306576E-6
6	0.0	4.6112839171460316E-5	0.0	4.6112839171460316E-5	1.317509690613152E-5
7	0.0	4.6112839171460316E-5	0.0	5.270038762452608E-5	1.9762645359197277E-5
8	0.0	7.246303298372335E-5	0.0	5.9287936077591834E-5	1.9762645359197277E-5
9	0.0	1.0540077524905216E-4	0.0	1.2516342060824942E-4	2.635019381226304E-5
10-11	0.0	1.0540077524905216E-4	0.0	1.317509690613152E-4	3.29377422653288E-5
12-13	0.0	1.0540077524905216E-4	0.0	1.6798248555317685E-4	3.6231516491861676E-5
14-15	0.0	1.5480738864704534E-4	0.0	1.943326793654399E-4	6.58754845306576E-5
16-17	3.29377422653288E-6	1.5810116287357821E-4	0.0	2.5032684121649885E-4	7.905058143678911E-5
18-19	6.58754845306576E-6	1.6798248555317685E-4	0.0	3.392587453328866E-4	8.234435566332199E-5
20-21	6.58754845306576E-6	1.8774513091237413E-4	0.0	4.314844236758072E-4	9.222567834292063E-5
22-23	6.58754845306576E-6	2.1080155049810429E-4	0.0	6.192295545881814E-4	9.55194525694535E-5
24-25	6.58754845306576E-6	2.3715174431036733E-4	0.0	0.0012911594968008887	1.0869454947558504E-4
26-27	6.58754845306576E-6	2.5032684121649885E-4	0.0	0.003056622482222512	1.1857587215518367E-4
28-29	6.58754845306576E-6	2.5032684121649885E-4	0.0	0.010062480262057947	1.2516342060824942E-4
30-31	6.58754845306576E-6	2.8326458348182766E-4	0.0	0.015632252479125044	1.317509690613152E-4
32-33	6.58754845306576E-6	2.8326458348182766E-4	0.0	0.028214470024480645	1.449260659674467E-4
34-35	9.88132267959864E-6	3.3596497110635376E-4	0.0	0.04311879839954193	1.5810116287357821E-4
36-37	1.317509690613152E-5	3.886653587308798E-4	0.0	0.0645942063565363	1.6468871132664398E-4
38-39	1.317509690613152E-5	4.0184045563701134E-4	0.0	0.10702131216850633	1.7127625977970972E-4
40-41	1.317509690613152E-5	4.677159401676689E-4	0.0	0.15076922144531604	1.778638082327755E-4
42-43	1.317509690613152E-5	4.841848113003333E-4	0.0	0.1826134306674359	1.8115758245930836E-4
44-45	1.317509690613152E-5	5.368851989248594E-4	0.0	0.22145361634671162	1.8445135668584126E-4
46-47	1.317509690613152E-5	5.665291669636553E-4	0.0	0.2575336192241528	1.943326793654399E-4
48-49	1.317509690613152E-5	5.665291669636553E-4	0.0	0.28770788491342053	2.2727042163076872E-4
50-51	1.317509690613152E-5	6.225233288147142E-4	0.0	0.31384727717518546	2.305641958573016E-4
52-53	1.317509690613152E-5	6.258171030412472E-4	0.0	0.37633017425251414	2.5691438966956464E-4
54-55	1.317509690613152E-5	6.2911087726778E-4	0.0	0.44739994073841416	2.8326458348182766E-4
56-57	1.317509690613152E-5	6.389921999473787E-4	0.0	0.5210948452828608	3.0302722884102494E-4
58-59	1.317509690613152E-5	6.422859741739115E-4	0.0	0.5745429196568098	3.2937742265328797E-4
60-61	1.317509690613152E-5	6.653423937596416E-4	0.0	0.6105274030816815	3.392587453328866E-4
62-63	1.317509690613152E-5	6.752237164392404E-4	0.0	0.6370324042825917	3.623151649186168E-4
64-65	1.317509690613152E-5	7.246303298372336E-4	0.0	0.6425890014027527	3.7549026182474827E-4
66-67	1.317509690613152E-5	7.279241040637664E-4	0.0	0.6470817094477435	3.8207781027781406E-4
68-69	1.317509690613152E-5	7.476867494229637E-4	0.0	0.6518873260442548	3.8207781027781406E-4
70-71	1.317509690613152E-5	7.608618463290952E-4	0.0	0.6560243064727802	3.886653587308798E-4
72-73	1.317509690613152E-5	7.641556205556281E-4	0.0	0.6596672207673255	4.2819064944927437E-4
74-75	1.317509690613152E-5	8.003871370474897E-4	0.0	0.662414228472254	4.314844236758072E-4
76-77	1.317509690613152E-5	8.563812988985487E-4	0.0	0.6652501680812988	4.347781979023401E-4
78-79	1.317509690613152E-5	8.893190411638775E-4	0.0	0.6670782127770245	4.413657463554059E-4
80-81	1.317509690613152E-5	8.893190411638775E-4	0.0	0.66768097346048	4.6112839171460313E-4
82-83	1.317509690613152E-5	8.893190411638775E-4	0.0	0.6682046835624987	4.94066133979932E-4
84-85	1.317509690613152E-5	8.959065896169433E-4	0.0	0.668478066823301	4.94066133979932E-4
86-87	1.317509690613152E-5	9.156692349761406E-4	0.0	0.6685505298562847	4.94066133979932E-4
88	1.317509690613152E-5	9.617820741476008E-4	0.0	0.6685505298562847	5.138287793391293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20850	0.0	52.097187	1
GTATCAA	38525	0.0	37.268074	1
TCAACGC	47645	0.0	29.703373	4
ATCAACG	48250	0.0	29.295462	3
CAACGCA	48820	0.0	29.008005	5
TATCAAC	49570	0.0	28.7829	2
AACGCAG	50105	0.0	28.298218	6
ACGCAGA	56910	0.0	24.907194	7
CGCAGAG	57410	0.0	24.638186	8
GTACATG	47440	0.0	21.210468	1
GCAGAGT	67175	0.0	20.853832	9
TACATGG	47240	0.0	20.793116	2
GAGTACT	40465	0.0	20.057306	12-13
ACATGGG	47965	0.0	20.041777	3
GTGGTAT	9840	0.0	19.564785	1
AGAGTAC	61295	0.0	18.917297	10-11
CAGAGTA	65540	0.0	18.765959	10-11
TGGTATC	9665	0.0	18.458305	2
CATGGGG	33895	0.0	18.188044	4
GTACTTT	46095	0.0	17.57997	14-15
>>END_MODULE