FastQCFastQC Report
Fri 27 May 2016
SRR2139250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10527338
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129100.055.4488371
GTATCAA240250.037.5481641
TCAACGC294100.030.0811964
ATCAACG298900.029.5835673
CAACGCA304100.029.0935295
TATCAAC309800.028.7674472
AACGCAG309300.028.6747556
ACGCAGA356650.024.8067917
GTACATG310500.023.6992591
TACATGG313400.023.105062
ACATGGG314800.022.421723
CGCAGAG394950.022.3902598
GCAGAGT427300.020.5841839
CATGGGG238200.020.4245664
GTGGTAT59150.020.2316251
GAGTACT263200.019.80008912-13
AGAGTAC383450.019.0734810-11
CAGAGTA417200.018.94400210-11
ATACAAC101250.018.868836
TATATAC133000.018.584443
Produced by FastQC (version 0.11.5)