##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10527338
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26747721028811	33.0	33.0	33.0	33.0	33.0
2	32.31793991985438	33.0	33.0	33.0	33.0	33.0
3	32.341513875587545	33.0	33.0	33.0	33.0	33.0
4	36.201305021269384	37.0	37.0	37.0	33.0	37.0
5	36.24905327443652	37.0	37.0	37.0	33.0	37.0
6	36.237832299105435	37.0	37.0	37.0	33.0	37.0
7	36.201457956417855	37.0	37.0	37.0	33.0	37.0
8	36.18762473476201	37.0	37.0	37.0	33.0	37.0
9	36.255812723026466	37.0	37.0	37.0	33.0	37.0
10-11	36.173471536679074	37.0	37.0	37.0	33.0	37.0
12-13	36.132061210535845	37.0	37.0	37.0	33.0	37.0
14-15	37.856064895038045	40.0	37.0	40.0	33.0	40.0
16-17	37.78433237348321	40.0	37.0	40.0	33.0	40.0
18-19	37.7478691669252	40.0	37.0	40.0	33.0	40.0
20-21	37.75487844797284	40.0	37.0	40.0	33.0	40.0
22-23	37.75539467475541	40.0	37.0	40.0	33.0	40.0
24-25	37.7507972414478	40.0	37.0	40.0	33.0	40.0
26-27	37.610911580850654	40.0	37.0	40.0	33.0	40.0
28-29	37.530424848121726	40.0	37.0	40.0	33.0	40.0
30-31	37.407258977440634	40.0	37.0	40.0	33.0	40.0
32-33	37.34818230092914	40.0	37.0	40.0	33.0	40.0
34-35	37.31133783440015	40.0	37.0	40.0	33.0	40.0
36-37	37.247537610152165	40.0	37.0	40.0	33.0	40.0
38-39	37.182256830384645	40.0	37.0	40.0	33.0	40.0
40-41	37.14510622459701	40.0	37.0	40.0	33.0	40.0
42-43	37.052129270050756	38.5	37.0	40.0	33.0	40.0
44-45	36.977833132055174	37.0	37.0	40.0	33.0	40.0
46-47	36.9209564589777	37.0	37.0	40.0	33.0	40.0
48-49	36.89143793457001	37.0	37.0	40.0	33.0	40.0
50-51	36.79274758879676	37.0	37.0	40.0	33.0	40.0
52-53	36.67004224359802	37.0	37.0	40.0	33.0	40.0
54-55	36.465651172385094	37.0	37.0	40.0	33.0	40.0
56-57	36.3524601612715	37.0	37.0	40.0	33.0	40.0
58-59	36.19221423846571	37.0	37.0	40.0	33.0	40.0
60-61	36.03970168364016	37.0	37.0	40.0	33.0	40.0
62-63	35.85923557557118	37.0	33.0	40.0	30.0	40.0
64-65	35.673939221683646	37.0	33.0	40.0	27.0	40.0
66-67	35.462009861863194	37.0	33.0	38.5	27.0	40.0
68-69	35.2444529641695	37.0	33.0	37.0	27.0	40.0
70-71	35.00842138305879	37.0	33.0	37.0	27.0	40.0
72-73	34.79633661626842	37.0	33.0	37.0	27.0	40.0
74-75	34.607034487294314	37.0	33.0	37.0	27.0	40.0
76-77	32.643107300271865	33.0	33.0	37.0	27.0	37.0
78-79	34.098102535436084	37.0	33.0	37.0	27.0	37.0
80-81	34.04037574225694	37.0	33.0	37.0	27.0	37.0
82-83	33.72414683545409	37.0	33.0	37.0	27.0	37.0
84-85	33.82514039019826	37.0	33.0	37.0	27.0	37.0
86-87	33.77120959011659	37.0	33.0	37.0	27.0	37.0
88-89	33.651859920612466	37.0	33.0	37.0	27.0	37.0
90-91	33.32400089715834	33.0	33.0	37.0	27.0	37.0
92-93	33.45856468760192	33.0	33.0	37.0	27.0	37.0
94-95	33.369673487387054	33.0	33.0	37.0	27.0	37.0
96-97	33.41691319730879	33.0	33.0	37.0	27.0	37.0
98-99	33.58496086585187	33.0	33.0	37.0	27.0	37.0
100	33.62618253748564	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	21.0
20	157.0
21	803.0
22	3161.0
23	8695.0
24	20227.0
25	39383.0
26	66399.0
27	102853.0
28	148061.0
29	201490.0
30	264794.0
31	341437.0
32	437528.0
33	564590.0
34	739178.0
35	1019670.0
36	1611963.0
37	2837956.0
38	2084445.0
39	34526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.719777592398	20.415911410842895	14.121499661167904	23.742811335591202
2	15.138404409547789	21.591355763441815	40.65232825240341	22.61791157460699
3	18.134080999394147	26.341910937028906	30.42452897399134	25.099479089585607
4	12.497185898277419	18.36363570733646	38.6412025528201	30.497975841566028
5	13.659550021097452	37.6130034012397	35.46772222949429	13.25972434816855
6	30.102681228625887	37.93848929330473	18.607581517758813	13.351247960310575
7	26.490229533809973	32.51404106147252	23.254330771938736	17.741398632778772
8	24.76412365595177	36.302273186250886	20.982151423275287	17.95145173452206
9	25.881331957486285	16.139605349237076	20.943576117521882	37.03548657575475
10-11	24.341743007605398	26.57833084965535	28.79748267857249	20.28244346416676
12-13	25.20767533242943	24.332095489355567	28.730109055879854	21.73012012233515
14-15	22.282824703761193	25.48807920220234	26.492683561667256	25.736412532369208
16-17	21.903794672499355	28.597903857556396	27.032446379132125	22.46585509081213
18-19	21.972435006836484	27.65553362113005	28.76779960897997	21.604231763053487
20-21	22.824292167172516	26.84363318561583	28.762550050584935	21.56952459662672
22-23	22.55731132355561	26.82801733863785	28.526795320942938	22.087876016863607
24-25	22.382225925389363	26.957012624910025	28.74449028898214	21.916271160718473
26-27	22.281599241907575	27.033993805895108	28.670992982815726	22.0134139693816
28-29	22.304264671737567	27.04218482137057	28.626247468774697	22.027303038117168
30-31	22.656527867787783	26.766227187214554	28.585308688283256	21.99193625671441
32-33	22.47426330141318	26.902358840318662	28.470201279931135	22.153176578337018
34-35	22.508079691136107	27.044334141979476	28.45466832811733	21.99291783876709
36-37	22.66883784874652	26.927962709612224	28.263129435879343	22.14007000576191
38-39	22.270404896635267	27.240738577936757	28.36452511056326	22.124331414864713
40-41	22.51755066545689	27.001426441681488	28.363559345136053	22.11746354772557
42-43	22.356845507840738	27.162688711731025	28.056766649617643	22.423699130810597
44-45	22.343772636381058	27.231629666886835	27.918291718940434	22.506305977791673
46-47	22.53886499043868	27.187686645506183	27.708051915763825	22.56539644829131
48-49	22.614130528301875	27.470322812052007	27.509513367162935	22.406033292483187
50-51	22.574197640104895	27.42992553883064	27.513057606322267	22.482819214742193
52-53	22.805827591436856	27.359671894017357	27.398589354856096	22.43591115968969
54-55	22.81722957108486	27.27164158707617	27.39366715711714	22.517461684721827
56-57	22.777649875585844	27.545550294001654	27.15171231086069	22.52508751955181
58-59	22.574639961708986	27.47497231022867	27.357183568358522	22.59320415970382
60-61	22.770515661401387	27.493831423786137	27.325476354076862	22.410176560735614
62-63	22.647474867931646	27.498974821281124	27.41720252979712	22.436347780990108
64-65	22.684680535855446	27.401227752867925	27.320634153619768	22.593457557656865
66-67	22.743252225681616	27.54225994599922	27.198201519175097	22.516286309144064
68-69	22.694501789793676	27.563700841806998	27.175356974032074	22.566440394367255
70-71	22.693888698013513	27.40024489039809	27.084456242214706	22.821410169373692
72-73	22.660142706424832	27.498372827275276	26.95153280978707	22.88995165651282
74-75	22.58810047700035	27.525773284602323	27.112729429810358	22.773396808586973
76-77	22.758029102887473	27.37992428433343	26.978296349256333	22.883750263522764
78-79	22.536788965167652	27.45586962886571	26.97388775514663	23.03345365082001
80-81	22.78279888772429	27.52625568178029	26.958511644291335	22.732433786204084
82-83	22.939521153451857	27.26581088987488	27.099481007390754	22.69518694928251
84-85	22.710182500403434	27.400444888639807	27.07986336877794	22.80950924217882
86-87	22.583438511895185	27.625047302028268	27.068231617921974	22.723282568154577
88-89	22.859254052127227	27.496094857727893	26.963994711346963	22.680656378797913
90-91	22.897514914395398	27.525702238158722	26.979954426868336	22.59682842057755
92-93	22.750782937895778	27.427612172193815	27.097206951766683	22.724397938143724
94-95	22.612627696561095	27.725596513127908	27.14124751608031	22.520528274230692
96-97	22.75275765434549	27.631874055228174	27.272243422000937	22.343124868425406
98-99	23.716321013032058	29.674098950158417	27.469451548735435	19.140128488074087
100	22.631433551686545	30.739061362743698	21.43237252903083	25.197132556538932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4226.0
1	3426.5
2	3844.0
3	5272.0
4	6534.0
5	10037.0
6	13549.0
7	16671.5
8	19084.0
9	19209.5
10	17037.5
11	14276.5
12	14477.5
13	18522.5
14	29036.0
15	43942.5
16	55919.0
17	62487.0
18	63816.0
19	60498.0
20	54294.0
21	48043.5
22	44383.0
23	45310.0
24	51102.5
25	62232.5
26	77337.0
27	93293.0
28	109923.5
29	128914.5
30	148467.0
31	168378.5
32	190376.0
33	212162.0
34	233994.5
35	255001.5
36	277875.5
37	299295.5
38	315437.5
39	331177.5
40	348095.0
41	362101.5
42	370330.5
43	377938.5
44	387633.5
45	402464.0
46	416816.0
47	431964.5
48	439748.5
49	434934.5
50	431366.5
51	428148.0
52	406201.5
53	373943.0
54	352674.0
55	332951.5
56	310303.5
57	282306.5
58	251779.0
59	220684.5
60	187302.0
61	160253.0
62	132262.5
63	106191.0
64	87468.5
65	69135.0
66	56090.0
67	41910.5
68	27107.0
69	19405.5
70	13902.0
71	8690.5
72	5832.0
73	4187.5
74	3184.5
75	2345.0
76	1529.0
77	870.0
78	626.5
79	495.5
80	338.0
81	223.5
82	181.0
83	115.0
84	63.5
85	43.0
86	35.5
87	32.5
88	25.0
89	16.5
90	11.0
91	9.0
92	9.0
93	7.5
94	5.0
95	3.5
96	3.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.749538772289823E-5
10-11	0.0026169958635316923
12-13	2.707237100205199E-4
14-15	0.003400669760959513
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	295.0
20-21	993.0
22-23	1980.0
24-25	3830.0
26-27	5228.0
28-29	8454.0
30-31	16536.0
32-33	21079.0
34-35	29432.0
36-37	38885.0
38-39	43895.0
40-41	50336.0
42-43	62203.0
44-45	74034.0
46-47	71563.0
48-49	62163.0
50-51	51834.0
52-53	49021.0
54-55	53054.0
56-57	56232.0
58-59	38864.0
60-61	61970.0
62-63	61456.0
64-65	67236.0
66-67	71153.0
68-69	71414.0
70-71	71758.0
72-73	69520.0
74-75	73016.0
76-77	67360.0
78-79	79411.0
80-81	82115.0
82-83	79650.0
84-85	82890.0
86-87	91050.0
88-89	101060.0
90-91	107383.0
92-93	120587.0
94-95	155130.0
96-97	413156.0
98-99	685498.0
100-101	7174614.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.23470825661249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1955284288978	74.24163786573695
2	7.339240890196012	12.217591486409322
3	1.878377672945666	4.690386528101016
4	0.67282941040082	2.240110387247234
5	0.32965943135951487	1.3719553296624996
6	0.1704378234249516	0.8511805505200729
7	0.11154927142947348	0.6499339744568933
8	0.06507930155109967	0.4333485342519912
9	0.04771908948599347	0.35746960424740654
>10	0.18370478475316204	2.4792630563550713
>50	0.0045579522767989315	0.2540218792338055
>100	0.0012507152788954722	0.16396624307982943
>500	4.3485340322026766E-5	0.03100092667092921
>1k	2.17426594359235E-5	0.018133634027017193
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	8.549169790121681E-5	0.0	0.0	0.0
5	0.0	9.499077544579645E-5	0.0	0.0	0.0
6	0.0	9.499077544579645E-5	0.0	0.0	0.0
7	0.0	9.499077544579645E-5	0.0	0.0	0.0
8	0.0	1.0448985299037611E-4	0.0	0.0	9.499077544579646E-6
9	0.0	1.7098339580243362E-4	0.0	9.499077544579646E-6	1.8998155089159292E-5
10-11	9.499077544579646E-6	1.7098339580243362E-4	0.0	9.499077544579646E-6	1.8998155089159292E-5
12-13	1.8998155089159292E-5	1.8048247334701326E-4	0.0	9.499077544579646E-6	1.8998155089159292E-5
14-15	1.8998155089159292E-5	2.5172555493136064E-4	0.0	2.3747693861449113E-5	2.8497232633738938E-5
16-17	1.8998155089159292E-5	2.5647509370365046E-4	0.0	2.8497232633738938E-5	2.8497232633738938E-5
18-19	1.8998155089159292E-5	2.897218651096792E-4	0.0	3.7996310178318584E-5	3.324677140602876E-5
20-21	1.8998155089159292E-5	3.2296863651570795E-4	0.0	6.649354281205752E-5	3.7996310178318584E-5
22-23	2.8497232633738938E-5	3.7521356301089604E-4	0.0	1.1398893053495576E-4	4.2745848950608405E-5
24-25	2.8497232633738938E-5	4.274584895060841E-4	0.0	1.5198524071327433E-4	4.7495387722898226E-5
26-27	2.8497232633738938E-5	4.55955722139823E-4	0.0	2.9922094265425885E-4	4.7495387722898226E-5
28-29	3.7996310178318584E-5	4.607052609121128E-4	0.0	9.166609830519358E-4	4.7495387722898226E-5
30-31	5.6994465267477876E-5	5.224492649518806E-4	0.0	0.003144194667255863	4.7495387722898226E-5
32-33	5.6994465267477876E-5	5.319483424964602E-4	0.0	0.008145458994477047	4.7495387722898226E-5
34-35	5.6994465267477876E-5	6.554363505759957E-4	0.0	0.014315109859681527	5.6994465267477876E-5
36-37	7.599262035663717E-5	7.931729749724005E-4	0.0	0.023467471073884014	5.6994465267477876E-5
38-39	7.599262035663717E-5	8.216702076061394E-4	0.0	0.04649323504194508	5.6994465267477876E-5
40-41	8.074215912892699E-5	9.879040646362833E-4	0.0	0.06941450915701576	5.6994465267477876E-5
42-43	8.549169790121681E-5	0.001021150836042312	0.0	0.08310742943752733	5.6994465267477876E-5
44-45	8.549169790121681E-5	0.001101892995171239	0.0	0.10216732853072638	5.6994465267477876E-5
46-47	8.549169790121681E-5	0.0011636369992110065	0.0	0.12060503804475547	5.6994465267477876E-5
48-49	8.549169790121681E-5	0.0011683865379832964	0.0	0.1384680533673375	5.6994465267477876E-5
50-51	8.549169790121681E-5	0.0013251213174688605	0.0	0.15603659728603755	5.6994465267477876E-5
52-53	8.549169790121681E-5	0.0013393699337857301	0.0	0.2008057497536414	6.17440040397677E-5
54-55	8.549169790121681E-5	0.0013536185501025995	0.0	0.25155457153555816	6.649354281205752E-5
56-57	9.024123667350663E-5	0.0013868653215086282	0.0	0.30672521391447677	6.649354281205752E-5
58-59	9.974031421808627E-5	0.0014058634765977875	0.0	0.4557467424338422	7.124308158434735E-5
60-61	1.0448985299037611E-4	0.001448609325548396	0.0	0.5738535230843733	8.549169790121681E-5
62-63	1.0448985299037611E-4	0.0014723570194098451	0.0	0.5807688515368272	9.499077544579645E-5
64-65	1.0448985299037611E-4	0.0016148431825785399	0.0	0.5855848838519291	9.499077544579645E-5
66-67	1.0448985299037611E-4	0.0016433404152122787	0.0	0.5898689678245346	9.499077544579645E-5
68-69	1.0448985299037611E-4	0.001690835802935177	0.0	0.5945567625927846	9.974031421808627E-5
70-71	1.0448985299037611E-4	0.0017288321131134956	0.0	0.5982139074474477	1.0448985299037611E-4
72-73	1.1398893053495575E-4	0.0017288321131134956	0.0	0.601134873792406	1.0448985299037611E-4
74-75	1.1398893053495575E-4	0.001743080729430365	0.0	0.603723372423304	1.0923939176266593E-4
76-77	1.1398893053495575E-4	0.001852320121193031	0.0	0.6060316482666368	1.1873846930724559E-4
78-79	1.1398893053495575E-4	0.001909314586460509	0.0	0.6075609997513142	1.234880080795354E-4
80-81	1.1398893053495575E-4	0.0019140641252327986	0.0	0.6080264545509986	1.472357019409845E-4
82-83	1.1398893053495575E-4	0.0019235632027773783	0.0	0.6086533936689409	1.7098339580243362E-4
84-85	1.1398893053495575E-4	0.0019473108966388275	0.0	0.6089051192238721	1.7098339580243362E-4
86-87	1.1398893053495575E-4	0.0019758081292725665	0.0	0.6089811118442288	1.7098339580243362E-4
88	1.1398893053495575E-4	0.0020707989047183627	0.0	0.6089858613830011	1.7098339580243362E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12910	0.0	55.448837	1
GTATCAA	24025	0.0	37.548164	1
TCAACGC	29410	0.0	30.081196	4
ATCAACG	29890	0.0	29.583567	3
CAACGCA	30410	0.0	29.093529	5
TATCAAC	30980	0.0	28.767447	2
AACGCAG	30930	0.0	28.674755	6
ACGCAGA	35665	0.0	24.806791	7
GTACATG	31050	0.0	23.699259	1
TACATGG	31340	0.0	23.10506	2
ACATGGG	31480	0.0	22.42172	3
CGCAGAG	39495	0.0	22.390259	8
GCAGAGT	42730	0.0	20.584183	9
CATGGGG	23820	0.0	20.424566	4
GTGGTAT	5915	0.0	20.231625	1
GAGTACT	26320	0.0	19.800089	12-13
AGAGTAC	38345	0.0	19.07348	10-11
CAGAGTA	41720	0.0	18.944002	10-11
ATACAAC	10125	0.0	18.86883	6
TATATAC	13300	0.0	18.58444	3
>>END_MODULE