FastQCFastQC Report
Fri 27 May 2016
SRR2139249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14895399
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169850.046.636651
GTATCAA306100.035.9298061
TCAACGC364850.029.5638284
ATCAACG365400.029.5193313
CAACGCA372550.028.9663335
AACGCAG383500.028.2857696
TATCAAC386650.028.1875612
ACGCAGA441350.024.490167
CGCAGAG446350.024.215828
GTACATG415250.022.1146321
TACATGG407550.021.8008122
ACATGGG412050.020.9124853
GCAGAGT534500.020.0855589
CATGGGG300400.018.8308584
GAGTACT305450.018.78214812-13
AGAGTAC473600.018.61651810-11
CAGAGTA519050.017.76492710-11
GTGGTAT96050.017.051881
AGTACTT333500.016.53505512-13
GTACTTT350900.016.30063614-15
Produced by FastQC (version 0.11.5)