##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14895399
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.320243318087684	33.0	33.0	33.0	33.0	33.0
2	32.370468424511486	33.0	33.0	33.0	33.0	33.0
3	32.39913922413223	33.0	33.0	33.0	33.0	33.0
4	36.26691826113554	37.0	37.0	37.0	33.0	37.0
5	36.31141025493845	37.0	37.0	37.0	33.0	37.0
6	36.29040202279912	37.0	37.0	37.0	33.0	37.0
7	36.25722607363522	37.0	37.0	37.0	33.0	37.0
8	36.24202607798556	37.0	37.0	37.0	33.0	37.0
9	36.30535610358608	37.0	37.0	37.0	33.0	37.0
10-11	36.226105759234784	37.0	37.0	37.0	33.0	37.0
12-13	36.18581069899504	37.0	37.0	37.0	33.0	37.0
14-15	37.9606377445814	40.0	37.0	40.0	33.0	40.0
16-17	37.887532888511416	40.0	37.0	40.0	33.0	40.0
18-19	37.85068842398918	40.0	37.0	40.0	33.0	40.0
20-21	37.86313471627856	40.0	37.0	40.0	33.0	40.0
22-23	37.86603159757969	40.0	37.0	40.0	33.0	40.0
24-25	37.86147061239724	40.0	37.0	40.0	33.0	40.0
26-27	37.728524480366914	40.0	37.0	40.0	33.0	40.0
28-29	37.659839547456755	40.0	37.0	40.0	33.0	40.0
30-31	37.54761430934968	40.0	37.0	40.0	33.0	40.0
32-33	37.49380173069325	40.0	37.0	40.0	33.0	40.0
34-35	37.45951508412436	40.0	37.0	40.0	33.0	40.0
36-37	37.39717396697123	40.0	37.0	40.0	33.0	40.0
38-39	37.33868051002358	40.0	37.0	40.0	33.0	40.0
40-41	37.32049823341433	40.0	37.0	40.0	33.0	40.0
42-43	37.231258579243544	40.0	37.0	40.0	33.0	40.0
44-45	37.17354297634281	40.0	37.0	40.0	33.0	40.0
46-47	37.127096584045304	40.0	37.0	40.0	33.0	40.0
48-49	37.116692920824164	40.0	37.0	40.0	33.0	40.0
50-51	37.01880472673414	38.5	37.0	40.0	33.0	40.0
52-53	36.919818237013445	37.0	37.0	40.0	33.0	40.0
54-55	36.733467007732045	37.0	37.0	40.0	33.0	40.0
56-57	36.622920248727574	37.0	37.0	40.0	33.0	40.0
58-59	36.49369797109807	37.0	37.0	40.0	33.0	40.0
60-61	36.35158934953551	37.0	37.0	40.0	33.0	40.0
62-63	36.193876999278714	37.0	37.0	40.0	33.0	40.0
64-65	36.03357961101105	37.0	37.0	40.0	33.0	40.0
66-67	35.834465816065105	37.0	33.0	40.0	33.0	40.0
68-69	35.616298553128104	37.0	33.0	38.5	30.0	40.0
70-71	35.387430185514546	37.0	33.0	37.0	27.0	40.0
72-73	35.17270002197958	37.0	33.0	37.0	27.0	40.0
74-75	34.95889324114731	37.0	33.0	37.0	27.0	40.0
76-77	32.964076697672944	33.0	33.0	37.0	27.0	37.0
78-79	34.37019674046586	37.0	33.0	37.0	27.0	37.0
80-81	34.28553250313581	37.0	33.0	37.0	27.0	37.0
82-83	33.96414585406677	37.0	33.0	37.0	27.0	37.0
84-85	34.069553505475916	37.0	33.0	37.0	27.0	37.0
86-87	34.005133230266594	37.0	33.0	37.0	27.0	37.0
88-89	33.884679473558734	37.0	33.0	37.0	27.0	37.0
90-91	33.56056743607487	37.0	33.0	37.0	27.0	37.0
92-93	33.72111292749288	37.0	33.0	37.0	27.0	37.0
94-95	33.60584726742916	33.0	33.0	37.0	27.0	37.0
96-97	33.63275885528902	33.0	33.0	37.0	27.0	37.0
98-99	33.797305438419315	33.0	33.0	37.0	27.0	37.0
100	33.832462712656536	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	28.0
20	199.0
21	1018.0
22	3968.0
23	10996.0
24	25291.0
25	48422.0
26	83031.0
27	129560.0
28	186992.0
29	258150.0
30	341838.0
31	442757.0
32	569819.0
33	738636.0
34	972889.0
35	1336816.0
36	2107381.0
37	3987501.0
38	3568714.0
39	81389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.904181217300724	20.6287793969131	14.178760837490826	24.28827854829535
2	14.311083576881694	21.320550057101524	41.88251687652006	22.485849489496722
3	17.618386724652357	26.792071833725302	30.915747876240175	24.67379356538217
4	12.11116264827817	18.746614306874225	39.15678257427008	29.985440470577522
5	13.05852901288512	38.03421445776646	36.00691730379293	12.900339225555488
6	29.206152852971577	38.43926570882727	19.127752133393674	13.226829304807477
7	25.758719185702915	32.80738569003758	23.599374545119602	17.834520579139905
8	23.967501642621322	37.04457329407557	21.596890422337797	17.39103464096531
9	25.28047762412477	16.765678251567014	21.207285875936822	36.74655824837139
10-11	23.74421674679339	26.809017850147438	29.449362928246742	19.997402474812432
12-13	24.794399838258308	24.315767421596618	29.249600655721174	21.6402320844239
14-15	21.931467123467467	25.759876118629904	26.6014463344656	25.707210423437022
16-17	21.64769470089388	28.95546470423518	27.285737696586708	22.11110289828423
18-19	21.72987779649273	28.080603950253362	28.83715300274937	21.352365250504533
20-21	22.383377476107512	27.210307589535983	28.725911976287104	21.6804029580694
22-23	22.07400409511215	27.42827091124135	28.522919434258142	21.974805559388358
24-25	22.02750525965819	27.532559880754775	28.568618437818134	21.871316421768896
26-27	22.008367138247444	27.65291859029586	28.570506218951973	21.76820805250472
28-29	21.922350033310188	27.519234991936887	28.68464293193935	21.87377204281357
30-31	21.974418306516654	27.302041172031068	28.751561675104952	21.97197884634732
32-33	21.722713058723375	27.632821705818394	28.586087440260954	22.058377795197277
34-35	22.008735941924442	27.533857521397888	28.63727553811192	21.820130998565755
36-37	21.916237127348793	27.591981362762024	28.641834047306848	21.849947462582335
38-39	21.79648930652286	27.5136622390504	28.634214180494922	22.05563427393182
40-41	22.054341936420414	27.36143676785408	28.67731206325504	21.90690923247047
42-43	22.035996775335352	27.38137966157545	28.521647960889823	22.060975602199377
44-45	21.902973342336853	27.629175499445346	28.33290807974765	22.134943078470155
46-47	22.149006085323077	27.4507681866257	28.077948141236952	22.322277586814263
48-49	22.0023959101634	27.637631522955175	28.005873798855124	22.354098768026304
50-51	21.849464727192604	27.75174715933964	27.959132716760728	22.439655396707025
52-53	21.898263020679	27.658557165355663	28.030984138017512	22.412195675947828
54-55	22.022323071174736	27.494795189597575	28.046026018174896	22.436855721052794
56-57	22.056613255963338	27.386576538440092	27.94006676653254	22.616743439064027
58-59	21.74004644077263	27.393723605351173	28.20541752727392	22.660812426602277
60-61	22.07134091974495	27.671951775726246	28.098110509288887	22.15859679523992
62-63	21.597600794112573	27.925137331052497	28.06906205540429	22.40819981943064
64-65	21.495625493334384	28.07955848306217	27.805851908777584	22.618964114825864
66-67	21.592707915670065	28.433253671851833	27.483681698079394	22.49035671439871
68-69	21.713717236584863	28.3746201116062	27.318609805788064	22.59305284602088
70-71	21.73033221377928	27.93132166168047	27.298115650501348	23.0402304740389
72-73	21.895778189021232	27.54181988688098	27.296522659396206	23.26587926470158
74-75	21.99391539836146	27.636060377449105	27.389462223610337	22.98056200057909
76-77	22.34178054016539	27.434832000235616	26.887177686097967	23.336209773501025
78-79	22.407199293399838	27.539277643036197	26.63377552859469	23.419747534969275
80-81	22.296963018219763	27.85583781609536	26.726614523161324	23.12058464252355
82-83	22.25941724617357	27.65769197916462	26.787274682061547	23.295616092600262
84-85	22.310608288676022	27.957323404805436	26.80177157156501	22.930296734953536
86-87	22.573487954605937	27.896698818607856	26.89452268862561	22.635290538160593
88-89	22.44402608567098	27.9514466721747	27.14538112221791	22.459146119936413
90-91	22.461978677443565	27.7250896093101	27.04670164377068	22.76623006947565
92-93	22.360620246070084	27.3660978408818	27.287675678560948	22.98560623448718
94-95	21.861295368657295	27.666732192466643	27.727971609500035	22.744000829376027
96-97	21.907855764066042	27.667717759446788	27.89872927675451	22.525697199732665
98-99	23.069429229055157	29.49926234757157	28.258243202782378	19.17306522059089
100	22.09865685012976	30.628347499191428	21.910548810979734	25.362446839699075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3046.0
1	2735.0
2	3843.0
3	5600.0
4	7477.5
5	12045.0
6	15921.0
7	18149.5
8	19283.5
9	18748.5
10	17153.5
11	15380.5
12	16461.5
13	21361.5
14	32681.0
15	49115.0
16	63196.0
17	71944.5
18	74789.0
19	71893.0
20	66063.0
21	60382.5
22	57294.5
23	60348.0
24	70536.0
25	87294.0
26	110744.0
27	138481.5
28	169389.0
29	204321.0
30	238832.5
31	271812.0
32	307330.5
33	341674.0
34	375175.5
35	407028.5
36	439404.5
37	471356.0
38	497155.0
39	518463.0
40	540105.0
41	560716.0
42	573084.0
43	579574.0
44	586906.5
45	599026.0
46	607838.5
47	612524.0
48	628080.5
49	630320.0
50	612205.0
51	594622.0
52	572593.0
53	542546.5
54	507497.5
55	465117.0
56	419006.5
57	373741.5
58	326518.5
59	278490.0
60	229148.5
61	183522.5
62	145075.0
63	110874.0
64	80984.0
65	59601.0
66	44340.5
67	33301.0
68	25163.0
69	18156.5
70	12853.0
71	9285.0
72	6591.0
73	4532.0
74	3075.0
75	2118.0
76	1421.5
77	925.0
78	644.0
79	436.5
80	292.0
81	199.5
82	144.0
83	96.5
84	63.5
85	48.5
86	47.0
87	42.5
88	28.0
89	16.5
90	12.0
91	10.0
92	8.5
93	6.5
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.6853929861160484E-5
10-11	0.0030344940743111345
12-13	2.920364872401203E-4
14-15	0.003383595162506221
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	344.0
20-21	1145.0
22-23	2558.0
24-25	5360.0
26-27	7317.0
28-29	12402.0
30-31	27961.0
32-33	35460.0
34-35	50090.0
36-37	68898.0
38-39	51513.0
40-41	85343.0
42-43	95317.0
44-45	111228.0
46-47	109306.0
48-49	101894.0
50-51	59049.0
52-53	25438.0
54-55	30658.0
56-57	30734.0
58-59	74552.0
60-61	125281.0
62-63	122831.0
64-65	129925.0
66-67	138713.0
68-69	124534.0
70-71	123836.0
72-73	120113.0
74-75	126668.0
76-77	119105.0
78-79	137136.0
80-81	139167.0
82-83	133724.0
84-85	137833.0
86-87	147134.0
88-89	161967.0
90-91	167291.0
92-93	182806.0
94-95	224589.0
96-97	561189.0
98-99	1002314.0
100-101	9782676.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.78494187918213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.19476676198678	67.90849689632789
2	8.701144757167802	13.71038367951631
3	2.620213744124579	6.193001626256674
4	1.036321613782408	3.2658615243994897
5	0.5299380788501715	2.08755703708881
6	0.3064644546735896	1.44868705496964
7	0.17799703371525463	0.981644016914621
8	0.11096591959648819	0.6993954820783456
9	0.07318652397431682	0.5189396433890328
>10	0.2443432013463202	2.8445234089005895
>50	0.0037108019724751305	0.19693363073042144
>100	8.78395316765983E-4	0.10719677919884046
>500	6.871349297498656E-5	0.03737922022940903
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6853929861160484E-5	0.0	0.0	0.0
3	0.0	2.6853929861160484E-5	0.0	0.0	0.0
4	0.0	8.056178958348145E-5	0.0	6.713482465290121E-6	0.0
5	0.0	1.0070223697935182E-4	0.0	1.3426964930580242E-5	0.0
6	6.042134218761109E-5	1.0070223697935182E-4	0.0	1.3426964930580242E-5	0.0
7	6.042134218761109E-5	1.1412920190993206E-4	0.0	2.6853929861160484E-5	0.0
8	6.713482465290121E-5	1.5441009670167277E-4	0.0	2.6853929861160484E-5	0.0
9	6.713482465290121E-5	2.6853929861160483E-4	0.0	3.3567412326450604E-5	0.0
10-11	1.0405897821199688E-4	2.6853929861160483E-4	0.0	3.692415355909566E-5	0.0
12-13	1.1412920190993206E-4	2.718960398442499E-4	0.0	4.028089479174072E-5	0.0
14-15	1.3426964930580241E-4	3.994522066847622E-4	0.0	4.363763602438578E-5	0.0
16-17	1.946909914934135E-4	4.1287917161534244E-4	0.0	5.706460095496603E-5	0.0
18-19	2.0140447395870364E-4	4.363763602438579E-4	0.0	6.377808342025615E-5	0.0
20-21	2.1483143888928387E-4	4.900842199661788E-4	0.0	9.063201328141663E-5	0.0
22-23	2.3497188628515423E-4	5.270083735252745E-4	0.0	1.443398730037376E-4	0.0
24-25	2.685392986116049E-4	5.605757858517251E-4	0.0	3.591713118930214E-4	0.0
26-27	2.75252781076895E-4	5.773594920149504E-4	0.0	7.754072247410089E-4	3.3567412326450605E-6
28-29	2.8196626354218507E-4	5.974999394108207E-4	0.0	0.0031989743947107427	6.713482465290121E-6
30-31	2.9539322847276536E-4	6.780617289943022E-4	0.0	0.012332667288737952	6.713482465290121E-6
32-33	3.155336758686357E-4	6.847752114595923E-4	0.0	0.03290277756238688	6.713482465290121E-6
34-35	3.4238760572979617E-4	7.519100361124936E-4	0.0	0.05807162332475955	6.713482465290121E-6
36-37	3.6924153559095665E-4	8.257583432306849E-4	0.0	0.09886609952509497	6.713482465290121E-6
38-39	3.6924153559095665E-4	8.828229441856509E-4	0.0	0.24795576137302533	6.713482465290121E-6
40-41	3.960954654521171E-4	0.001000308887328228	0.0	0.40657521158043497	6.713482465290121E-6
42-43	4.0280894791740727E-4	0.0010238060759567434	0.0	0.4741900502296045	6.713482465290121E-6
44-45	4.1287917161534244E-4	0.0011278650541687403	0.0	0.5667219790486981	6.713482465290121E-6
46-47	4.1959265408063253E-4	0.0012084268437522218	0.0	0.6634834018209247	6.713482465290121E-6
48-49	4.263061365459227E-4	0.0012117835849848669	0.0	0.7507351766810677	6.713482465290121E-6
50-51	4.330196190112128E-4	0.0013057723394989285	0.0	0.828433665993103	6.713482465290121E-6
52-53	4.5316006640708315E-4	0.0013091290807315736	0.0	1.2398996495495018	6.713482465290121E-6
54-55	4.598735488723733E-4	0.0013561234579886044	0.0	1.7832587096189905	6.713482465290121E-6
56-57	4.632302901050183E-4	0.001382977387849765	0.0	2.3808627080080234	1.3426964930580242E-5
58-59	4.733005138029535E-4	0.0014333285063394409	0.0	3.0043337543358186	2.014044739587036E-5
60-61	4.900842199661787E-4	0.0014736094011311815	0.0	3.188041488516018	2.014044739587036E-5
62-63	5.370785972232097E-4	0.001500463330992342	0.0	3.197561206651799	2.014044739587036E-5
64-65	5.370785972232097E-4	0.0016448032039960796	0.0	3.205385770465095	2.014044739587036E-5
66-67	5.437920796884998E-4	0.0016917975812531104	0.0	3.209762961032464	2.014044739587036E-5
68-69	5.5050556215379E-4	0.0017052245461836907	0.0	3.2144959661704933	2.014044739587036E-5
70-71	5.706460095496603E-4	0.0017421486997427865	0.0	3.2188966539264907	2.014044739587036E-5
72-73	5.974999394108208E-4	0.001775716112069237	0.0	3.222162763145855	2.014044739587036E-5
74-75	5.974999394108208E-4	0.0018193537480936228	0.0	3.225069701053325	2.014044739587036E-5
76-77	6.042134218761109E-4	0.0018495644191874284	0.0	3.2280941249039383	2.014044739587036E-5
78-79	6.310673517372714E-4	0.0018764183490485888	0.0	3.2299940404416154	2.014044739587036E-5
80-81	6.310673517372714E-4	0.001886488572746524	0.0	3.2306183943108877	2.3497188628515425E-5
82-83	6.377808342025615E-4	0.001886488572746524	0.0	3.2311789700967393	2.6853929861160484E-5
84-85	6.444943166678516E-4	0.001899915537677104	0.0	3.231464293101514	3.0210671093805544E-5
86-87	6.478510579004966E-4	0.001940196432468845	0.0	3.231588492527122	4.028089479174072E-5
88	6.71348246529012E-4	0.0020274717045176164	0.0	3.2316019194920527	4.028089479174072E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16985	0.0	46.63665	1
GTATCAA	30610	0.0	35.929806	1
TCAACGC	36485	0.0	29.563828	4
ATCAACG	36540	0.0	29.519331	3
CAACGCA	37255	0.0	28.966333	5
AACGCAG	38350	0.0	28.285769	6
TATCAAC	38665	0.0	28.187561	2
ACGCAGA	44135	0.0	24.49016	7
CGCAGAG	44635	0.0	24.21582	8
GTACATG	41525	0.0	22.114632	1
TACATGG	40755	0.0	21.800812	2
ACATGGG	41205	0.0	20.912485	3
GCAGAGT	53450	0.0	20.085558	9
CATGGGG	30040	0.0	18.830858	4
GAGTACT	30545	0.0	18.782148	12-13
AGAGTAC	47360	0.0	18.616518	10-11
CAGAGTA	51905	0.0	17.764927	10-11
GTGGTAT	9605	0.0	17.05188	1
AGTACTT	33350	0.0	16.535055	12-13
GTACTTT	35090	0.0	16.300636	14-15
>>END_MODULE