FastQCFastQC Report
Fri 27 May 2016
SRR2139248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10911959
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137250.053.191091
GTATCAA245200.036.6242681
TCAACGC307850.028.6710914
ATCAACG311500.028.3351383
TATCAAC320750.027.7045542
CAACGCA318950.027.701365
AACGCAG327500.027.017326
ACGCAGA372700.023.6952977
GTGGTAT62700.022.359891
TGGTATC60550.022.1655652
CGCAGAG403000.021.9137388
GTACATG315450.021.5983771
CTTATAC184400.021.0469071
TACATGG316200.020.9929242
ACATGGG317950.020.5681843
TATACAC220150.019.8618773
GCAGAGT443400.019.7444659
GAGTACT270650.018.94451112-13
CAGAGTA429250.018.64758710-11
AGAGTAC400750.018.48037710-11
Produced by FastQC (version 0.11.5)