##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10911959
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25175039605629	33.0	33.0	33.0	33.0	33.0
2	32.30393946678135	33.0	33.0	33.0	33.0	33.0
3	32.32673903925042	33.0	33.0	33.0	33.0	33.0
4	36.18085900066157	37.0	37.0	37.0	33.0	37.0
5	36.22731830279054	37.0	37.0	37.0	33.0	37.0
6	36.221938608823585	37.0	37.0	37.0	33.0	37.0
7	36.18386111971279	37.0	37.0	37.0	33.0	37.0
8	36.16829416239559	37.0	37.0	37.0	33.0	37.0
9	36.24074540602654	37.0	37.0	37.0	33.0	37.0
10-11	36.15459231472552	37.0	37.0	37.0	33.0	37.0
12-13	36.11254954312054	37.0	37.0	37.0	33.0	37.0
14-15	37.812881215920996	40.0	37.0	40.0	33.0	40.0
16-17	37.73226457320817	40.0	37.0	40.0	33.0	40.0
18-19	37.69522516534382	40.0	37.0	40.0	33.0	40.0
20-21	37.705229993868194	40.0	37.0	40.0	33.0	40.0
22-23	37.703476581920256	40.0	37.0	40.0	33.0	40.0
24-25	37.6976863793048	40.0	37.0	40.0	33.0	40.0
26-27	37.545585815781756	40.0	37.0	40.0	33.0	40.0
28-29	37.45836829390451	40.0	37.0	40.0	33.0	40.0
30-31	37.339498859541195	40.0	37.0	40.0	33.0	40.0
32-33	37.27528769867867	40.0	37.0	40.0	33.0	40.0
34-35	37.230617899792875	40.0	37.0	40.0	33.0	40.0
36-37	37.16855136061646	40.0	37.0	40.0	33.0	40.0
38-39	37.08747872285086	40.0	37.0	40.0	33.0	40.0
40-41	37.05988141605333	37.0	37.0	40.0	33.0	40.0
42-43	36.96790291471544	37.0	37.0	40.0	33.0	40.0
44-45	36.89504160936843	37.0	37.0	40.0	33.0	40.0
46-47	36.83395969126168	37.0	37.0	40.0	33.0	40.0
48-49	36.812614442968545	37.0	37.0	40.0	33.0	40.0
50-51	36.700178843385764	37.0	37.0	40.0	33.0	40.0
52-53	36.57916100634774	37.0	37.0	40.0	33.0	40.0
54-55	36.369995078856775	37.0	37.0	40.0	33.0	40.0
56-57	36.24846660465206	37.0	37.0	40.0	33.0	40.0
58-59	36.09041244581279	37.0	37.0	40.0	33.0	40.0
60-61	35.930277935151835	37.0	33.0	40.0	30.0	40.0
62-63	35.753834641245646	37.0	33.0	40.0	27.0	40.0
64-65	35.571714379191526	37.0	33.0	40.0	27.0	40.0
66-67	35.36159753904112	37.0	33.0	38.5	27.0	40.0
68-69	35.144990159340246	37.0	33.0	37.0	27.0	40.0
70-71	34.909555765961215	37.0	33.0	37.0	27.0	40.0
72-73	34.7014711260632	37.0	33.0	37.0	27.0	40.0
74-75	34.51238686909598	37.0	33.0	37.0	27.0	38.5
76-77	32.5385211766221	33.0	30.0	37.0	27.0	37.0
78-79	34.006458189580435	37.0	33.0	37.0	27.0	37.0
80-81	33.9645829786858	37.0	33.0	37.0	27.0	37.0
82-83	33.645314360338936	37.0	33.0	37.0	27.0	37.0
84-85	33.755706225928066	37.0	33.0	37.0	27.0	37.0
86-87	33.70663460241324	37.0	33.0	37.0	27.0	37.0
88-89	33.59555191913478	35.0	33.0	37.0	27.0	37.0
90-91	33.266011082682155	33.0	33.0	37.0	27.0	37.0
92-93	33.41644147347962	33.0	33.0	37.0	27.0	37.0
94-95	33.33056435446896	33.0	33.0	37.0	27.0	37.0
96-97	33.375125146145706	33.0	33.0	37.0	27.0	37.0
98-99	33.54089676801951	33.0	33.0	37.0	27.0	37.0
100	33.58508040977508	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	26.0
20	164.0
21	921.0
22	3590.0
23	9929.0
24	22087.0
25	42300.0
26	71672.0
27	111132.0
28	157629.0
29	214684.0
30	282050.0
31	361554.0
32	463410.0
33	592032.0
34	776405.0
35	1064294.0
36	1678570.0
37	2908762.0
38	2105535.0
39	45211.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.60132933050793	20.03395540617409	14.096258976046371	24.268456287271604
2	15.007497737115763	21.18703891757658	40.71102173312784	23.09444161217981
3	18.022492569849284	26.123017874242375	30.4879994508777	25.366490105030636
4	12.55506000343293	18.403798987881096	37.96797623598109	31.07316477270488
5	13.78277722634405	37.27187757945205	35.33541502492815	13.609930169275748
6	30.194065062011322	37.58271085879264	18.481447740043745	13.7417763391523
7	26.484593646292108	32.39250624017191	22.779548566852203	18.34335154668378
8	24.74121282897049	36.180533669527165	20.906145266858132	18.17210823464421
9	25.495868934955386	16.348388868136933	20.733184774572038	37.42255742233564
10-11	24.279006455616315	26.44316358237469	28.577664363946774	20.70016559806222
12-13	25.287274211888054	24.077624303886093	28.42150795219997	22.21359353202588
14-15	22.436730970260683	25.29170680171196	26.03700090321322	26.234561324814145
16-17	22.232442405621207	28.487506230549435	26.56213700949573	22.717914354333626
18-19	22.23313430704789	27.3437840079861	28.305449094887546	22.117632590078465
20-21	23.059968789715956	26.533319189192472	28.254746669043374	22.1519653520482
22-23	22.766525040592803	26.765765200131803	28.023631129994587	22.444078629280806
24-25	22.659632610722642	26.963369757009602	28.164338486010955	22.2126591462568
26-27	22.609275740936386	27.004945094201858	28.144191451830018	22.241587713031734
28-29	22.536545426252445	26.758092775966492	28.179389329966753	22.525972467814313
30-31	23.003052917528134	26.68620410142785	28.070250963705018	22.240492017338994
32-33	22.796734365594833	26.810703426474525	28.12305106798022	22.26951113995042
34-35	22.80347099846448	26.928436062526927	28.108693870362846	22.15939906864575
36-37	22.901484457929552	26.65766352367213	27.91985896589005	22.520993052508267
38-39	22.43983167990198	26.845694896065275	28.147675230239948	22.566798193792792
40-41	22.664705500690715	26.60866341935678	28.18962777842567	22.537003301526838
42-43	22.60352098621609	26.73766878131389	27.992184412183647	22.666625820286374
44-45	22.46010402236398	26.941830191608297	27.78655625301443	22.811509533013293
46-47	22.89713501898067	26.7519967436858	27.424572267012792	22.926295970320737
48-49	22.797662746626802	26.970154007266856	27.279410500104373	22.95277274600197
50-51	22.64006493491998	27.113963870973002	27.352291837547398	22.89367935655962
52-53	22.846409836769926	27.086065877923122	27.33858131623044	22.728942969076513
54-55	22.84039754687557	27.004380719723358	27.191809292274975	22.963412441126092
56-57	22.96771260599907	27.207358614446527	26.906988315649333	22.917940463905072
58-59	22.66537046520753	27.313563932655537	27.194639577626194	22.82642602451074
60-61	22.884024037886043	27.421596933540783	26.924835490887222	22.76954353768596
62-63	22.803393726960884	27.49954366428672	26.95644200131666	22.740620607435737
64-65	22.814516948738124	27.4071913947617	26.87415430409132	22.904137352408863
66-67	22.895532361327252	27.40523406189828	26.69054530916817	23.0086882676063
68-69	22.87426170106707	27.287568263690588	26.79130642199266	23.046863613249684
70-71	22.899809187684713	27.132049126786807	26.80424605927419	23.163895626254288
72-73	22.906529432356766	27.15744503834687	26.648412944292154	23.2876125850042
74-75	22.81161905686508	27.28008597948553	26.6923072002336	23.21598776341579
76-77	23.06828812176939	27.212244775647516	26.514441179510655	23.205025923072448
78-79	22.857876681820237	27.267102588807372	26.618235543232906	23.256785186139485
80-81	23.021892258611402	27.22189994304317	26.587712549248145	23.168495249097287
82-83	23.050816244119044	27.180317074323746	26.744278053328813	23.024588628228397
84-85	23.043621840643034	27.232358185193778	26.695574241879378	23.02844573228381
86-87	22.87288529813711	27.325335631573626	26.749369573543518	23.052409496745746
88-89	22.98303110522217	27.23914905313279	26.677466590424615	23.100353251220426
90-91	23.030735929893385	27.270303270933866	26.669274680275045	23.0296861188977
92-93	23.012676731519	27.29761978548636	26.79199973890191	22.89770374409273
94-95	22.84892957393021	27.39533119832544	26.84133461577628	22.914404611968074
96-97	22.822149288530223	27.41443083167218	26.967349158867858	22.796070720929734
98-99	24.007116565252772	29.362580486990986	27.096502667860705	19.533800279895534
100	22.84570480169404	30.34530418359755	21.00658158301379	25.802409431694617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5403.0
1	4230.5
2	4097.0
3	5240.5
4	6303.5
5	9683.5
6	13076.5
7	16184.5
8	18783.0
9	18798.5
10	16528.5
11	14183.5
12	14923.0
13	19397.0
14	30084.0
15	45331.0
16	57909.5
17	64819.0
18	66199.5
19	62726.0
20	56522.0
21	50317.0
22	46537.0
23	47456.0
24	53743.5
25	65495.0
26	81626.0
27	98673.5
28	116652.5
29	137210.0
30	156763.5
31	175060.5
32	194628.5
33	213610.0
34	232884.0
35	251807.5
36	272806.5
37	293111.0
38	309328.0
39	326270.0
40	343907.5
41	359234.5
42	370746.0
43	381303.5
44	393620.5
45	411429.0
46	428407.5
47	446309.5
48	468608.0
49	476040.0
50	473157.5
51	472542.5
52	458777.5
53	433355.5
54	409457.5
55	383921.5
56	356684.5
57	327147.5
58	293871.0
59	256946.0
60	217814.0
61	183570.5
62	149919.5
63	119903.5
64	96278.5
65	75509.5
66	60872.0
67	45530.0
68	30618.0
69	22962.5
70	16500.0
71	9943.0
72	6603.5
73	4680.0
74	3401.0
75	2463.5
76	1643.0
77	978.5
78	701.0
79	551.5
80	384.0
81	266.5
82	210.0
83	139.5
84	86.5
85	57.5
86	42.5
87	34.5
88	25.5
89	15.0
90	11.0
91	10.0
92	8.5
93	6.5
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.74927719211555E-5
10-11	0.00272636654884792
12-13	3.80316678242651E-4
14-15	0.0033037147591921854
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	293.0
20-21	1053.0
22-23	2029.0
24-25	4011.0
26-27	6507.0
28-29	10540.0
30-31	19888.0
32-33	25490.0
34-35	35839.0
36-37	49487.0
38-39	53797.0
40-41	60537.0
42-43	73492.0
44-45	86585.0
46-47	84152.0
48-49	77486.0
50-51	64794.0
52-53	62248.0
54-55	72456.0
56-57	80289.0
58-59	81922.0
60-61	85191.0
62-63	87217.0
64-65	92584.0
66-67	99829.0
68-69	91787.0
70-71	91095.0
72-73	87504.0
74-75	92687.0
76-77	86539.0
78-79	100738.0
80-81	102995.0
82-83	99174.0
84-85	102163.0
86-87	109498.0
88-89	121150.0
90-91	126202.0
92-93	137334.0
94-95	170692.0
96-97	405997.0
98-99	679488.0
100-101	6989200.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.6571012870846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40672205687652	74.7950720285834
2	7.239439541393875	12.112610539522253
3	1.79914755353164	4.5153440734862045
4	0.6786107992061673	2.2708244945479903
5	0.31548550348935217	1.3196301360007807
6	0.16546159998379276	0.8305222697380334
7	0.10457402621778143	0.6123851932309426
8	0.06792304663159651	0.45457961534294716
9	0.04454473605818651	0.3353835146603542
>10	0.17184000908295657	2.2778971181518504
>50	0.004899225928077775	0.27673734030393526
>100	0.0013105799756563635	0.1752825929496612
>500	4.1321624441816E-5	0.02373108348164921
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.164257307051832E-6
2	9.164257307051832E-6	3.665702922820733E-5	0.0	0.0	9.164257307051832E-6
3	9.164257307051832E-6	3.665702922820733E-5	0.0	1.8328514614103665E-5	9.164257307051832E-6
4	9.164257307051832E-6	1.4662811691282932E-4	0.0	1.8328514614103665E-5	9.164257307051832E-6
5	9.164257307051832E-6	1.4662811691282932E-4	0.0	2.7492771921155495E-5	9.164257307051832E-6
6	1.0080683037757015E-4	1.4662811691282932E-4	0.0	2.7492771921155495E-5	1.8328514614103665E-5
7	1.0080683037757015E-4	1.4662811691282932E-4	0.0	4.582128653525916E-5	1.8328514614103665E-5
8	1.0080683037757015E-4	1.5579237421988113E-4	0.0	4.582128653525916E-5	1.8328514614103665E-5
9	1.0080683037757015E-4	3.024204911327105E-4	0.0	4.582128653525916E-5	2.7492771921155495E-5
10-11	1.0538895903109606E-4	3.024204911327105E-4	0.0	5.040341518878507E-5	3.665702922820733E-5
12-13	1.0997108768462198E-4	3.024204911327105E-4	0.0	6.414980114936282E-5	5.040341518878507E-5
14-15	1.1913534499167381E-4	3.6657029228207325E-4	0.0	9.622470172404423E-5	7.789618710994057E-5
16-17	1.8328514614103663E-4	3.8489880689617694E-4	0.0	1.3288173095225156E-4	1.0080683037757015E-4
18-19	1.8328514614103663E-4	4.123915788173324E-4	0.0	1.512102455663552E-4	1.0997108768462198E-4
20-21	1.8328514614103663E-4	4.536307366990657E-4	0.0	2.061957894086662E-4	1.145532163381479E-4
22-23	1.9244940344808847E-4	5.131984091949025E-4	0.0	3.024204911327104E-4	1.3288173095225156E-4
24-25	1.9244940344808847E-4	5.910945963048431E-4	0.0	5.131984091949027E-4	1.4662811691282932E-4
26-27	1.9244940344808847E-4	6.323337541865764E-4	0.0	9.85157660508072E-4	1.4662811691282932E-4
28-29	1.9244940344808847E-4	6.460801401471541E-4	0.0	0.004110169402212746	1.5579237421988113E-4
30-31	2.0161366075514028E-4	7.239763272570947E-4	0.0	0.013242351808689897	1.5579237421988113E-4
32-33	2.1077791806219212E-4	7.285584559106206E-4	0.0	0.031080578656866288	1.5579237421988113E-4
34-35	2.1077791806219212E-4	8.843508301305018E-4	0.0	0.05406911811160581	1.6495663152693297E-4
36-37	2.1077791806219212E-4	0.0010493074616574347	0.0	0.08642811066280583	1.6495663152693297E-4
38-39	2.1077791806219212E-4	0.0010813823622321161	0.0	0.14389716823532786	1.695387601804589E-4
40-41	2.1077791806219212E-4	0.0011638606779955827	0.0	0.2005322783929082	1.7412088883398481E-4
42-43	2.291064326762958E-4	0.0011776070639561605	0.0	0.24556085667110736	1.7412088883398481E-4
44-45	2.3368856132982172E-4	0.001315070923561938	0.0	0.30337357389264386	1.7412088883398481E-4
46-47	2.3827068998334762E-4	0.001420459882593034	0.0	0.35878525569973274	1.7412088883398481E-4
48-49	2.3827068998334762E-4	0.00142504201124656	0.0	0.40662726097119684	2.016136607551403E-4
50-51	2.474349472903995E-4	0.0016037450287340706	0.0	0.4457861324442293	2.016136607551403E-4
52-53	2.474349472903995E-4	0.0016220735433481743	0.0	0.5236731552968628	2.061957894086662E-4
54-55	2.474349472903995E-4	0.001635819929308752	0.0	0.6047951609788857	2.1077791806219212E-4
56-57	2.474349472903995E-4	0.0016587305725763815	0.0	0.6878828998532711	2.1077791806219212E-4
58-59	2.474349472903995E-4	0.0016724769585369592	0.0	0.7487473147580558	2.1077791806219212E-4
60-61	2.7034559055802903E-4	0.0016999697304581147	0.0	0.790430939119181	2.1077791806219212E-4
62-63	3.5740603497502147E-4	0.0017320446310327962	0.0	0.8239629566056837	2.1077791806219212E-4
64-65	3.5740603497502147E-4	0.001970315321016144	0.0	0.8327835542637212	2.1077791806219212E-4
66-67	3.5740603497502147E-4	0.0019886438356302473	0.0	0.8396934042732382	2.1536004671571804E-4
68-69	3.5740603497502147E-4	0.002016136607551403	0.0	0.8465849257681411	2.1994217536924396E-4
70-71	3.8948093554970286E-4	0.00205279363677961	0.0	0.8511395616497459	2.1994217536924396E-4
72-73	4.35302222084962E-4	0.002071122151393714	0.0	0.8548831607596765	2.4285281863687357E-4
74-75	4.3988435073848793E-4	0.0021123613092754474	0.0	0.8576461843377527	2.474349472903995E-4
76-77	4.4446647939201385E-4	0.0022040038823459658	0.0	0.8602992368281442	2.520170759439254E-4
78-79	4.4904860804553977E-4	0.0022544072975347507	0.0	0.8620954312603264	2.7034559055802903E-4
80-81	4.4904860804553977E-4	0.0022681536834953282	0.0	0.8626911079852848	2.7492771921155495E-4
82-83	4.582128653525916E-4	0.0022727358121488544	0.0	0.8632409634237079	2.7492771921155495E-4
84-85	4.7195925131316937E-4	0.002291064326762958	0.0	0.8635479660434942	2.7492771921155495E-4
86-87	4.7654137996669524E-4	0.0023093928413770617	0.0	0.8636396086165646	2.7492771921155495E-4
88	4.857056372737471E-4	0.00240103541444758	0.0	0.8636671013884858	2.840919765186068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13725	0.0	53.19109	1
GTATCAA	24520	0.0	36.624268	1
TCAACGC	30785	0.0	28.671091	4
ATCAACG	31150	0.0	28.335138	3
TATCAAC	32075	0.0	27.704554	2
CAACGCA	31895	0.0	27.70136	5
AACGCAG	32750	0.0	27.01732	6
ACGCAGA	37270	0.0	23.695297	7
GTGGTAT	6270	0.0	22.35989	1
TGGTATC	6055	0.0	22.165565	2
CGCAGAG	40300	0.0	21.913738	8
GTACATG	31545	0.0	21.598377	1
CTTATAC	18440	0.0	21.046907	1
TACATGG	31620	0.0	20.992924	2
ACATGGG	31795	0.0	20.568184	3
TATACAC	22015	0.0	19.861877	3
GCAGAGT	44340	0.0	19.744465	9
GAGTACT	27065	0.0	18.944511	12-13
CAGAGTA	42925	0.0	18.647587	10-11
AGAGTAC	40075	0.0	18.480377	10-11
>>END_MODULE