FastQCFastQC Report
Fri 27 May 2016
SRR2139247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15387946
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172950.051.278651
GTATCAA311650.036.640521
TCAACGC385200.028.9456964
ATCAACG394450.028.29943
CAACGCA396600.028.1047275
TATCAAC408900.027.5082842
AACGCAG408700.027.283116
ACGCAGA473150.023.5848837
GTACATG425400.021.600941
CGCAGAG519150.021.4789338
TACATGG425000.021.1890472
ACATGGG426900.020.3442173
GCAGAGT573600.019.3595859
GTGGTAT86900.018.82821
GAGTACT334300.018.65894712-13
AGAGTAC510400.018.38736310-11
CATGGGG332150.017.8519214
TGGTATC84050.017.789352
CAGAGTA554150.017.77996410-11
TATATAC177400.016.6159523
Produced by FastQC (version 0.11.5)