##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15387946
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30138291361303	33.0	33.0	33.0	33.0	33.0
2	32.33879057022945	33.0	33.0	33.0	33.0	33.0
3	32.36680977435195	33.0	33.0	33.0	33.0	33.0
4	36.22995915114337	37.0	37.0	37.0	33.0	37.0
5	36.27542454334061	37.0	37.0	37.0	33.0	37.0
6	36.26307143266554	37.0	37.0	37.0	33.0	37.0
7	36.22711679648473	37.0	37.0	37.0	33.0	37.0
8	36.21380904248039	37.0	37.0	37.0	33.0	37.0
9	36.277505327871566	37.0	37.0	37.0	33.0	37.0
10-11	36.19709219151146	37.0	37.0	37.0	33.0	37.0
12-13	36.15621568336671	37.0	37.0	37.0	33.0	37.0
14-15	37.90497620670101	40.0	37.0	40.0	33.0	40.0
16-17	37.83817924757469	40.0	37.0	40.0	33.0	40.0
18-19	37.799126309645224	40.0	37.0	40.0	33.0	40.0
20-21	37.80411975538044	40.0	37.0	40.0	33.0	40.0
22-23	37.80758699623435	40.0	37.0	40.0	33.0	40.0
24-25	37.804852583579354	40.0	37.0	40.0	33.0	40.0
26-27	37.670857891288335	40.0	37.0	40.0	33.0	40.0
28-29	37.598083038523654	40.0	37.0	40.0	33.0	40.0
30-31	37.48021564710221	40.0	37.0	40.0	33.0	40.0
32-33	37.427395445110925	40.0	37.0	40.0	33.0	40.0
34-35	37.39553224002712	40.0	37.0	40.0	33.0	40.0
36-37	37.338154866087145	40.0	37.0	40.0	33.0	40.0
38-39	37.27739705115396	40.0	37.0	40.0	33.0	40.0
40-41	37.24326528238688	40.0	37.0	40.0	33.0	40.0
42-43	37.152090912522766	40.0	37.0	40.0	33.0	40.0
44-45	37.08535951167345	40.0	37.0	40.0	33.0	40.0
46-47	37.03003130603322	37.0	37.0	40.0	33.0	40.0
48-49	37.00430426676256	37.0	37.0	40.0	33.0	40.0
50-51	36.907753355768065	37.0	37.0	40.0	33.0	40.0
52-53	36.782436028975795	37.0	37.0	40.0	33.0	40.0
54-55	36.575468753915686	37.0	37.0	40.0	33.0	40.0
56-57	36.46340010471803	37.0	37.0	40.0	33.0	40.0
58-59	36.3029277040096	37.0	37.0	40.0	33.0	40.0
60-61	36.148322999056546	37.0	37.0	40.0	33.0	40.0
62-63	35.96762707813272	37.0	35.0	40.0	33.0	40.0
64-65	35.77912530749603	37.0	33.0	40.0	30.0	40.0
66-67	35.56606631030199	37.0	33.0	40.0	27.0	40.0
68-69	35.34814645729965	37.0	33.0	37.0	27.0	40.0
70-71	35.1172054848263	37.0	33.0	37.0	27.0	40.0
72-73	34.91002035354466	37.0	33.0	37.0	27.0	40.0
74-75	34.72079698395624	37.0	33.0	37.0	27.0	40.0
76-77	32.72246132682001	33.0	33.0	37.0	27.0	37.0
78-79	34.1870554655821	37.0	33.0	37.0	27.0	37.0
80-81	34.14110693930361	37.0	33.0	37.0	27.0	37.0
82-83	33.826345514750344	37.0	33.0	37.0	27.0	37.0
84-85	33.9325325208119	37.0	33.0	37.0	27.0	37.0
86-87	33.88365799203294	37.0	33.0	37.0	27.0	37.0
88-89	33.7724190297196	37.0	33.0	37.0	27.0	37.0
90-91	33.449278677706715	33.0	33.0	37.0	27.0	37.0
92-93	33.59924470978101	33.0	33.0	37.0	27.0	37.0
94-95	33.51269507425849	33.0	33.0	37.0	27.0	37.0
96-97	33.55589179924244	33.0	33.0	37.0	27.0	37.0
98-99	33.71416625652152	33.0	33.0	37.0	27.0	37.0
100	33.76092781319694	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	31.0
20	198.0
21	1207.0
22	4429.0
23	12521.0
24	27811.0
25	53069.0
26	90760.0
27	140141.0
28	201962.0
29	275654.0
30	361878.0
31	471186.0
32	606728.0
33	784630.0
34	1037488.0
35	1432897.0
36	2283878.0
37	4159935.0
38	3358405.0
39	83132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29563191864593	19.899302999893553	13.629687808886255	24.17537727257426
2	15.147668181315426	21.45704176502829	40.37330908231677	23.021980971339513
3	18.144097984227393	26.160431028286684	30.252920045339383	25.442550942146536
4	12.565712148976868	18.09869881269404	38.63631962316478	30.69926941516431
5	13.700925386663041	37.463180596032764	35.47404572384124	13.36184829346295
6	30.24269775836229	37.788786105695976	18.642748031478664	13.325768104463066
7	26.641931288295396	32.34226972202788	23.252388590394066	17.76341039928266
8	24.72569113512616	36.27931889025345	21.010373964140502	17.984616010479893
9	25.66219855867694	16.369183799396218	21.045929134787002	36.922688507139846
10-11	24.26269666334298	26.52538318860464	28.88509960047511	20.326820547577267
12-13	25.24483752941474	24.158624286075007	28.75754037658854	21.83899780792172
14-15	22.369655144087908	25.427108860151403	26.35383065066432	25.849405345096365
16-17	22.16379950904429	28.495706964399275	26.856765028938884	22.483728497617548
18-19	22.196581662035985	27.64728313967309	28.449791154712916	21.706344043578003
20-21	22.97510204986167	26.810867979638576	28.40987823034417	21.804151740155586
22-23	22.72315853203174	26.711578339834475	28.176352086719174	22.38891104141461
24-25	22.527506157471542	26.879298540529806	28.342555550026123	22.250639751972532
26-27	22.462910208938347	26.976319392956928	28.245425613300807	22.31534478480392
28-29	22.526653577962094	26.96812593944512	28.233173248207947	22.272047234384836
30-31	22.79993889533448	26.75188639483125	28.140030656562466	22.308144053271803
32-33	22.55717369347537	26.91050951912984	28.07394512540588	22.458371661988917
34-35	22.726107377760265	26.98625855181139	28.046533027512794	22.241101042915556
36-37	22.826453423758117	26.85185270279833	27.86184825079223	22.459845622651322
38-39	22.48399470526567	27.20294233972325	27.970457901537	22.34260505347408
40-41	22.753254896865915	26.91924780135895	27.921444660994748	22.406052640780388
42-43	22.54020195696334	27.081275001470985	27.69406325335696	22.684459788208716
44-45	22.552891654932665	27.16260779710752	27.570629327045708	22.713871220914108
46-47	22.780945335426082	27.103949894311786	27.35252188612544	22.76258288413669
48-49	22.74398540943533	27.33675092339578	27.192893892033943	22.726369775134945
50-51	22.71498669442398	27.33736085811475	27.219190315675274	22.728462131785996
52-53	22.95657388361651	27.244199239639627	27.060040701407495	22.73918617533637
54-55	22.940722094264125	27.10708598340596	27.12014576795748	22.83204615437244
56-57	22.929009872038026	27.329213686020502	26.959732422921174	22.7820440190203
58-59	22.764601241880438	27.296485829019346	27.081578844183475	22.85733408491674
60-61	22.93595744894756	27.269576392513876	27.045404326478096	22.74906183206047
62-63	22.87304926071763	27.3616849137462	27.082893382706512	22.68237244282966
64-65	22.88768167552323	27.267354386834803	27.028754561030183	22.816209376611788
66-67	22.934970020992164	27.38567867808811	26.792139142842768	22.887212158076952
68-69	22.854179151144898	27.30815324461936	26.877390248734777	22.960277355500963
70-71	22.91615977741493	27.157239154946588	26.860254002188494	23.06634706544999
72-73	22.899126970291693	27.207494624613215	26.732130979758395	23.161247425336697
74-75	22.770746655657238	27.262925288730926	26.802654450668562	23.163673604943273
76-77	22.98050285594045	27.180046987588824	26.647729811243444	23.191720345227285
78-79	22.787353983116105	27.258548522675806	26.740841206727474	23.213256287480615
80-81	22.926930053068283	27.23605520511323	26.681280503912973	23.155734237905516
82-83	23.01236204985512	27.137060133669305	26.82060506746972	23.02997274900585
84-85	22.938843674454297	27.226968396229402	26.80258214459892	23.03160578471738
86-87	22.818241511291024	27.32779233091315	26.78685420569391	23.06711195210191
88-89	22.882091742385256	27.20304776457757	26.738640310254613	23.17622018278256
90-91	22.92634348854705	27.15854015977275	26.79821713514447	23.11689921653573
92-93	22.906849114700044	27.24100649235728	26.892550705475166	22.959593687467514
94-95	22.71418897762791	27.339774174492952	26.93745099111044	23.008585856768697
96-97	22.707529817940863	27.361993907132998	27.05533466876313	22.875141606163005
98-99	23.89843913550273	29.333263422778177	27.176551844873707	19.59174559684539
100	22.786032270216165	30.315417240911263	20.990210347637635	25.908340141234937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5322.0
1	4467.5
2	4964.5
3	6652.0
4	8431.5
5	13103.5
6	17359.0
7	20467.0
8	22833.0
9	22700.0
10	20844.0
11	18872.5
12	19818.5
13	25272.0
14	38230.5
15	56495.0
16	71670.5
17	80175.5
18	81733.0
19	77547.0
20	71068.5
21	65384.0
22	62609.5
23	65869.0
24	76866.5
25	95950.5
26	121595.5
27	149001.5
28	177660.5
29	208048.0
30	235468.5
31	260798.5
32	287848.0
33	316723.0
34	344668.5
35	372256.0
36	405194.5
37	435044.0
38	458205.5
39	480974.0
40	504403.0
41	524065.5
42	539515.5
43	556315.5
44	573122.0
45	596401.0
46	619341.5
47	641029.0
48	653462.0
49	650376.0
50	646047.5
51	639283.5
52	614680.0
53	575987.0
54	545029.0
55	517532.5
56	485806.0
57	446313.0
58	401377.5
59	352539.5
60	299488.5
61	253244.0
62	207194.5
63	165202.5
64	132653.5
65	104322.5
66	86384.5
67	65860.5
68	43714.0
69	32537.0
70	23289.5
71	13784.0
72	8945.0
73	6314.0
74	4616.5
75	3299.0
76	2177.5
77	1279.0
78	928.5
79	746.0
80	516.0
81	342.5
82	258.5
83	160.0
84	92.0
85	65.5
86	46.5
87	39.0
88	30.0
89	19.5
90	12.0
91	6.5
92	4.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.498593119575543E-6
10-11	0.0027229105171021526
12-13	2.4369724198408286E-4
14-15	0.00347024872585334
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	382.0
20-21	1257.0
22-23	2583.0
24-25	5426.0
26-27	7881.0
28-29	13585.0
30-31	26110.0
32-33	33888.0
34-35	47908.0
36-37	66836.0
38-39	75318.0
40-41	84265.0
42-43	102921.0
44-45	122614.0
46-47	120308.0
48-49	110105.0
50-51	95640.0
52-53	92925.0
54-55	101041.0
56-57	107621.0
58-59	115181.0
60-61	113171.0
62-63	115585.0
64-65	125920.0
66-67	133644.0
68-69	136776.0
70-71	136907.0
72-73	130715.0
74-75	136625.0
76-77	130772.0
78-79	148814.0
80-81	151147.0
82-83	144801.0
84-85	148946.0
86-87	158836.0
88-89	173963.0
90-91	181381.0
92-93	194841.0
94-95	238376.0
96-97	566241.0
98-99	951305.0
100-101	9835385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.92841273790773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7494597679219	73.59851829900566
2	7.622923781511464	12.643139392455902
3	1.9205313312930905	4.777998447526705
4	0.7609557707349471	2.524194169232015
5	0.350934907033313	1.455123740729893
6	0.192809730254791	0.9593642934272382
7	0.11091671912075798	0.643870322394656
8	0.07827653055506864	0.5193078746849743
9	0.042758838526184666	0.3191330348543693
>10	0.16465008870564296	2.101567191138628
>50	0.00435397233677462	0.24107928942501866
>100	0.0013691467252159525	0.17877758476338076
>500	5.941528081864075E-5	0.03792636036145547
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.599437247830217E-5	0.0	0.0	0.0
3	0.0	3.249296559787772E-5	0.0	0.0	0.0
4	0.0	1.1047608303278424E-4	0.0	0.0	0.0
5	0.0	1.2347326927193532E-4	0.0	0.0	6.498593119575543E-6
6	0.0	1.2347326927193532E-4	0.0	0.0	1.2997186239151086E-5
7	0.0	1.2347326927193532E-4	0.0	0.0	1.2997186239151086E-5
8	0.0	1.4296904863066195E-4	0.0	6.498593119575543E-6	1.949577935872663E-5
9	0.0	2.0795497982641737E-4	0.0	6.498593119575543E-6	1.949577935872663E-5
10-11	0.0	2.1445357294599292E-4	0.0	6.498593119575543E-6	1.949577935872663E-5
12-13	0.0	2.2095216606556848E-4	0.0	1.2997186239151086E-5	2.27450759185144E-5
14-15	3.2492965597877714E-6	3.3142824909835266E-4	0.0	1.2997186239151086E-5	2.9243669038089944E-5
16-17	1.2997186239151086E-5	3.41176138777716E-4	0.0	2.599437247830217E-5	3.249296559787772E-5
18-19	1.2997186239151086E-5	3.541733250168671E-4	0.0	2.599437247830217E-5	3.574226215766549E-5
20-21	1.2997186239151086E-5	3.671705112560182E-4	0.0	3.574226215766549E-5	4.224085527724103E-5
22-23	1.949577935872663E-5	4.094113665332592E-4	0.0	4.54901518370288E-5	4.54901518370288E-5
24-25	1.949577935872663E-5	4.7764659428880244E-4	0.0	5.198874495660434E-5	4.873944839681657E-5
26-27	2.599437247830217E-5	5.166381530062557E-4	0.0	1.2997186239151084E-4	5.848733807617989E-5
28-29	2.599437247830217E-5	5.198874495660435E-4	0.0	8.870579608220616E-4	5.848733807617989E-5
30-31	2.599437247830217E-5	5.913719738813745E-4	0.0	0.0031745627389126525	5.848733807617989E-5
32-33	2.9243669038089944E-5	5.913719738813745E-4	0.0	0.007635846915501263	5.848733807617989E-5
34-35	3.899155871745326E-5	7.148452431533097E-4	0.0	0.013494328612798614	6.498593119575544E-5
36-37	3.899155871745326E-5	8.285706227458817E-4	0.0	0.023121994319449782	6.498593119575544E-5
38-39	3.899155871745326E-5	8.643128849035472E-4	0.0	0.04313116253462288	6.498593119575544E-5
40-41	3.899155871745326E-5	9.585424851373927E-4	0.0	0.06330279557778537	6.498593119575544E-5
42-43	3.899155871745326E-5	9.877861541754825E-4	0.0	0.07528945058684246	7.473382087511875E-5
44-45	3.899155871745326E-5	0.0011112594234474178	0.0	0.09263419562298958	8.448171055448205E-5
46-47	3.899155871745326E-5	0.001208738320241051	0.0	0.10965726029971772	8.448171055448205E-5
48-49	3.899155871745326E-5	0.0012119876168008386	0.0	0.12704749548770186	9.09803036740576E-5
50-51	4.873944839681657E-5	0.001364704555110864	0.0	0.14450271660688177	9.09803036740576E-5
52-53	5.198874495660434E-5	0.0013906989275891662	0.0	0.1921374041733705	1.1047608303278424E-4
54-55	5.198874495660434E-5	0.0014004468172685294	0.0	0.2469822808060283	1.2997186239151087E-4
56-57	5.198874495660434E-5	0.001423191893187044	0.0	0.3124556064857519	1.3322115895129862E-4
58-59	5.848733807617989E-5	0.0014459369691055585	0.0	0.37106966712776346	1.364704555110864E-4
60-61	6.173663463596766E-5	0.0014751806381436484	0.0	0.3914005157023556	1.364704555110864E-4
62-63	7.798311743490651E-5	0.0015141721968611015	0.0	0.42332160510571065	1.5596623486981303E-4
64-65	7.798311743490651E-5	0.0016376454661330368	0.0	0.4321239494861757	1.5596623486981303E-4
66-67	7.798311743490651E-5	0.001660390542051551	0.0	0.43749828599606466	1.5596623486981303E-4
68-69	8.448171055448205E-5	0.0017091299904483678	0.0	0.44295710421650814	1.5596623486981303E-4
70-71	8.448171055448205E-5	0.0017318750663668824	0.0	0.44776606312499406	1.5596623486981303E-4
72-73	9.747889679363315E-5	0.0017546201422853967	0.0	0.45178219367289174	1.7221271766875188E-4
74-75	9.747889679363315E-5	0.001777365218203911	0.0	0.45496975359804354	1.7546201422853966E-4
76-77	9.747889679363315E-5	0.0018423511493996666	0.0	0.4583490220202228	1.7546201422853966E-4
78-79	9.747889679363315E-5	0.0018715948184377564	0.0	0.460597535239596	1.7871131078832744E-4
80-81	9.747889679363315E-5	0.0018748441149975441	0.0	0.4613611199311461	1.85209903907903E-4
82-83	9.747889679363315E-5	0.0018975891909160587	0.0	0.4618387665254349	1.8845920046769074E-4
84-85	9.747889679363315E-5	0.0019365807496335118	0.0	0.46217344407109306	1.8845920046769074E-4
86-87	9.747889679363315E-5	0.001962575122111814	0.0	0.46230016663692475	1.8845920046769074E-4
88	9.747889679363315E-5	0.0020860483913837493	0.0	0.46230341593348456	1.8845920046769074E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17295	0.0	51.27865	1
GTATCAA	31165	0.0	36.64052	1
TCAACGC	38520	0.0	28.945696	4
ATCAACG	39445	0.0	28.2994	3
CAACGCA	39660	0.0	28.104727	5
TATCAAC	40890	0.0	27.508284	2
AACGCAG	40870	0.0	27.28311	6
ACGCAGA	47315	0.0	23.584883	7
GTACATG	42540	0.0	21.60094	1
CGCAGAG	51915	0.0	21.478933	8
TACATGG	42500	0.0	21.189047	2
ACATGGG	42690	0.0	20.344217	3
GCAGAGT	57360	0.0	19.359585	9
GTGGTAT	8690	0.0	18.8282	1
GAGTACT	33430	0.0	18.658947	12-13
AGAGTAC	51040	0.0	18.387363	10-11
CATGGGG	33215	0.0	17.851921	4
TGGTATC	8405	0.0	17.78935	2
CAGAGTA	55415	0.0	17.779964	10-11
TATATAC	17740	0.0	16.615952	3
>>END_MODULE