FastQCFastQC Report
Fri 27 May 2016
SRR2139246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16571239
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA288850.053.364221
GTATCAA541750.037.210361
CTTATAC136350.029.1145361
TATCAAC705250.028.2631472
TCAACGC702100.028.2348824
ATCAACG703500.028.1429773
TATACAC158400.028.1282793
CAACGCA704200.028.1226485
AACGCAG725750.027.4664676
ACGCAGA810600.024.4728727
CGCAGAG820600.024.1798888
TTATACA177800.023.7638112
GTACATG667850.022.6164231
TACATGG696950.021.497872
GCAGAGT923250.021.3068649
ACATGGG689550.021.1273673
GAGTACT556250.020.25606212-13
TACACAT226250.019.5460175
CAGAGTA904750.019.26369510-11
ACACATC212650.018.9252196
Produced by FastQC (version 0.11.5)