##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16571239
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28515375343992	33.0	33.0	33.0	33.0	33.0
2	32.33248726905695	33.0	33.0	33.0	33.0	33.0
3	32.346562619729276	33.0	33.0	33.0	33.0	33.0
4	36.21496751087834	37.0	37.0	37.0	33.0	37.0
5	36.268021902285035	37.0	37.0	37.0	33.0	37.0
6	36.256881214494584	37.0	37.0	37.0	33.0	37.0
7	36.22130451440595	37.0	37.0	37.0	33.0	37.0
8	36.21010686044659	37.0	37.0	37.0	33.0	37.0
9	36.26530647466976	37.0	37.0	37.0	33.0	37.0
10-11	36.18622267773701	37.0	37.0	37.0	33.0	37.0
12-13	36.14480791086291	37.0	37.0	37.0	33.0	37.0
14-15	37.8432448533269	40.0	37.0	40.0	33.0	40.0
16-17	37.77641457588054	40.0	37.0	40.0	33.0	40.0
18-19	37.76932237836893	40.0	37.0	40.0	33.0	40.0
20-21	37.784030328949925	40.0	37.0	40.0	33.0	40.0
22-23	37.784170012105136	40.0	37.0	40.0	33.0	40.0
24-25	37.77459896665141	40.0	37.0	40.0	33.0	40.0
26-27	37.62675657279595	40.0	37.0	40.0	33.0	40.0
28-29	37.55356303794048	40.0	37.0	40.0	33.0	40.0
30-31	37.421630903818624	40.0	37.0	40.0	33.0	40.0
32-33	37.36033743197274	40.0	37.0	40.0	33.0	40.0
34-35	37.323922896149114	40.0	37.0	40.0	33.0	40.0
36-37	37.265634897014294	40.0	37.0	40.0	33.0	40.0
38-39	37.19792842857334	40.0	37.0	40.0	33.0	40.0
40-41	37.18391933853544	40.0	37.0	40.0	33.0	40.0
42-43	37.09677450090271	40.0	37.0	40.0	33.0	40.0
44-45	37.028217388035635	37.0	37.0	40.0	33.0	40.0
46-47	36.977936582695136	37.0	37.0	40.0	33.0	40.0
48-49	36.96732540614774	37.0	37.0	40.0	33.0	40.0
50-51	36.88158995481942	37.0	37.0	40.0	33.0	40.0
52-53	36.780350415168044	37.0	37.0	40.0	33.0	40.0
54-55	36.581045229861374	37.0	37.0	40.0	33.0	40.0
56-57	36.46874683326631	37.0	37.0	40.0	33.0	40.0
58-59	36.33150030820056	37.0	37.0	40.0	33.0	40.0
60-61	36.18488475746159	37.0	37.0	40.0	33.0	40.0
62-63	36.0078190997818	37.0	35.0	40.0	33.0	40.0
64-65	35.837822256110556	37.0	33.0	40.0	33.0	40.0
66-67	35.63734815979704	37.0	33.0	40.0	27.0	40.0
68-69	35.41709874556745	37.0	33.0	37.0	27.0	40.0
70-71	35.19648933471609	37.0	33.0	37.0	27.0	40.0
72-73	34.986639269577154	37.0	33.0	37.0	27.0	40.0
74-75	34.78989388882677	37.0	33.0	37.0	27.0	40.0
76-77	32.794615762935095	33.0	33.0	37.0	27.0	37.0
78-79	34.248623996386144	37.0	33.0	37.0	27.0	37.0
80-81	34.20200592576699	37.0	33.0	37.0	27.0	37.0
82-83	33.893254277842956	37.0	33.0	37.0	27.0	37.0
84-85	33.99926077357528	37.0	33.0	37.0	27.0	37.0
86-87	33.951547124057335	37.0	33.0	37.0	27.0	37.0
88-89	33.84256553557931	37.0	33.0	37.0	27.0	37.0
90-91	33.52082343712658	37.0	33.0	37.0	27.0	37.0
92-93	33.66830307409967	33.0	33.0	37.0	27.0	37.0
94-95	33.57569557057471	33.0	33.0	37.0	27.0	37.0
96-97	33.611829234229376	33.0	33.0	37.0	27.0	37.0
98-99	33.77207357454457	33.0	33.0	37.0	27.0	37.0
100	33.82293907370446	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	3.0
18	12.0
19	54.0
20	301.0
21	1346.0
22	4731.0
23	13151.0
24	28892.0
25	55660.0
26	94656.0
27	146574.0
28	212276.0
29	291961.0
30	388042.0
31	505311.0
32	655878.0
33	853311.0
34	1122388.0
35	1532809.0
36	2420968.0
37	4487855.0
38	3657571.0
39	97486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.260572006715975	20.985938347760236	14.222744599845552	23.530745045678238
2	14.7423134745688	21.637325971823834	41.54449766852074	22.075862885086625
3	17.353771797027367	27.933753173193626	31.014995318092993	23.69747971168601
4	12.035038538759837	18.888291937615527	39.42790880030154	29.648760723323104
5	12.713853200717217	38.533195978888486	35.7672651996631	12.985685620731196
6	28.395004139400804	39.03376808457111	19.1086858381561	13.462541937871997
7	25.61424646642294	33.295488647529616	23.87417742270207	17.216087463345378
8	24.42675529572653	36.22878772070091	21.583763290119705	17.760693693452858
9	26.031030640204584	16.294018900463296	21.54083395358487	36.13411650574725
10-11	23.88922450314866	26.85200962067167	29.190632677955676	20.068133198223993
12-13	25.105571816715088	24.8332195213088	28.880691325375423	21.180517336600694
14-15	22.431297358202766	25.870450110861665	26.92778032679585	24.77047220413972
16-17	21.320098635955947	28.767595470682668	27.69006288546077	22.222243007900616
18-19	21.290212518206996	27.735714269765825	29.84684488588934	21.127228326137836
20-21	22.282645806896152	26.907085298378895	29.667678666073837	21.142590228651116
22-23	22.120262781538173	26.966481139850686	29.386439692819984	21.526816385791157
24-25	21.74938032192799	27.33957132680496	29.648891205913287	21.262157145353758
26-27	21.679221190967315	27.38168754361783	29.679975622345474	21.259115643069382
28-29	21.471915281832644	27.1666891367789	29.84352337808614	21.51787220330232
30-31	21.99489443296313	27.277347948159232	29.47907984038413	21.248677778493512
32-33	21.728024441928177	27.360886130342994	29.38098872541663	21.530100702312197
34-35	21.619626610375338	27.489960856890644	29.613272531635342	21.27714000109868
36-37	21.960635364139293	27.243215734363126	29.210423708730865	21.58572519276672
38-39	21.610503396594034	27.484863990264273	29.315469138704884	21.589163474436806
40-41	21.782446384236913	27.237241944976265	29.466059326865192	21.514252343921626
42-43	21.8581958740363	27.312024267162695	28.97711296237322	21.852666896427788
44-45	21.834158932962822	27.770182512893705	28.63770712356558	21.757951430577897
46-47	22.15231392361481	27.53443471534487	28.122674256914497	22.190577104125822
48-49	22.118423998360154	27.627104543371843	28.02098316667359	22.233488291594412
50-51	21.962399290994856	27.718264032044914	27.91445441664415	22.40488226031608
52-53	22.08367798751794	27.7725554061877	27.91890640803575	22.22486019825861
54-55	22.212450760072862	27.80685882134342	27.701857821082697	22.278832597501022
56-57	22.321851247058994	27.95364446732453	27.432365563306966	22.292138722309513
58-59	21.96170203667335	28.013322317019185	27.944682154055194	22.08029349225227
60-61	22.31514694991274	28.313858036871505	27.40512712364322	21.96586788957254
62-63	22.080435496242252	28.30155339471183	27.499911254794046	22.118099854251877
64-65	22.089219755299755	28.04499908121584	27.51983975154827	22.345941411936142
66-67	22.253159100120794	28.098009658589422	27.26261211740634	22.386219123883443
68-69	22.102268093840717	27.985002911047037	27.57419083641124	22.33853815870101
70-71	22.220791758920765	27.599329378351968	27.70634568939651	22.473533173330758
72-73	22.29571599361873	27.767850463034037	27.58479070641855	22.35164283692868
74-75	22.198389716366933	27.974901251228633	27.431668028401496	22.395041004002934
76-77	22.20597871047735	27.827002565624138	27.464586510726967	22.502432213171545
78-79	22.151485306217467	27.775930301490913	27.476665532545354	22.595918859746263
80-81	22.117337133851777	27.88502494013786	27.49627266253286	22.501365263477506
82-83	22.390363387146575	27.645154818021567	27.55358919902407	22.410892595807784
84-85	22.467843066290204	27.629410152978362	27.381574345516952	22.52117243521448
86-87	22.012765790220254	27.974264993374696	27.626646948993834	22.386322267411213
88-89	22.339721415978666	27.55517369284519	27.539104589060802	22.56600030211534
90-91	22.435710480752313	27.462146128316046	27.660824730934014	22.441318659997627
92-93	22.43909235740543	27.71725240815203	27.47521427115406	22.36844096328848
94-95	22.246770170236953	27.41119277544954	27.66967236392079	22.67236469039272
96-97	22.321634341495237	27.58269326330173	27.694583956488756	22.401088438714275
98-99	23.263498080492354	29.547353424059114	28.01264944751745	19.17649904793108
100	22.498946111255773	30.377313218140234	21.71196233382585	25.41177833677814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4839.0
1	4592.0
2	7561.0
3	11313.5
4	15243.0
5	25647.0
6	34355.0
7	39398.0
8	42177.0
9	40525.0
10	36202.5
11	31285.0
12	32012.0
13	41053.5
14	64932.0
15	100444.0
16	129877.0
17	145570.0
18	147085.0
19	135876.5
20	118172.0
21	99083.5
22	83844.0
23	77419.5
24	81081.5
25	96555.5
26	122829.0
27	153184.5
28	180827.5
29	214463.5
30	252868.0
31	290565.0
32	341593.0
33	396509.0
34	446925.5
35	502343.0
36	566604.0
37	616430.5
38	630813.5
39	633999.5
40	641777.0
41	637626.0
42	627231.0
43	639672.5
44	663431.5
45	679100.5
46	682453.0
47	682193.0
48	680200.5
49	667456.0
50	653583.0
51	637720.5
52	615386.0
53	588475.0
54	559571.5
55	516882.0
56	466101.5
57	414411.5
58	360145.5
59	310686.0
60	258869.5
61	207690.5
62	161168.0
63	121727.5
64	92273.0
65	69627.5
66	52273.0
67	40072.0
68	30158.0
69	20499.5
70	13480.5
71	9173.0
72	6254.0
73	4186.0
74	2800.5
75	1851.5
76	1196.5
77	770.5
78	502.0
79	351.5
80	265.0
81	191.0
82	130.5
83	87.5
84	59.5
85	43.0
86	33.5
87	23.0
88	12.5
89	6.0
90	3.5
91	2.0
92	2.0
93	1.5
94	1.5
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.017275895906154E-5
10-11	0.0027909802037131928
12-13	3.3190034854967696E-4
14-15	0.0035483164535856375
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	590.0
20-21	1773.0
22-23	3552.0
24-25	9725.0
26-27	11145.0
28-29	18480.0
30-31	37079.0
32-33	45641.0
34-35	63390.0
36-37	84315.0
38-39	104061.0
40-41	115125.0
42-43	143077.0
44-45	172539.0
46-47	163976.0
48-49	143635.0
50-51	128729.0
52-53	124614.0
54-55	139684.0
56-57	149804.0
58-59	152554.0
60-61	155584.0
62-63	154231.0
64-65	165249.0
66-67	170529.0
68-69	162983.0
70-71	162910.0
72-73	156725.0
74-75	164170.0
76-77	159156.0
78-79	178882.0
80-81	182327.0
82-83	174529.0
84-85	179413.0
86-87	188229.0
88-89	205863.0
90-91	213855.0
92-93	224324.0
94-95	259617.0
96-97	557714.0
98-99	964127.0
100-101	9977334.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.63206306409003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.91928621394888	66.60804222633023
2	7.499087618616277	11.493411106258785
3	2.4516141050909677	5.636167401304311
4	1.179002139972639	3.613974653723215
5	0.625810143473365	2.3978561190399073
6	0.38776465921975467	1.7829123491612173
7	0.24520762865957402	1.3153536524265514
8	0.1615132195642349	0.9901672981864537
9	0.11751406107187616	0.8104810445079609
>10	0.40664871303714817	4.672289311870757
>50	0.005052670455166531	0.2508623335184099
>100	0.0012592207444536805	0.16858022940049935
>500	9.21673373956727E-5	0.04730612035823043
>1k	1.474388082700451E-4	0.2125961539134393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.034551791812308E-6	0.0
3	0.0	0.0	0.0	6.034551791812308E-6	0.0
4	0.0	0.0	0.0	6.034551791812308E-6	0.0
5	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
6	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
7	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
8	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
9	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
10-11	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
12-13	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
14-15	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
16-17	0.0	0.0	0.0	1.2069103583624616E-5	6.034551791812308E-6
18-19	0.0	0.0	0.0	1.8103655375436923E-5	6.034551791812308E-6
20-21	0.0	0.0	0.0	2.1120931271343076E-5	6.034551791812308E-6
22-23	0.0	0.0	0.0	3.319003485496769E-5	6.034551791812308E-6
24-25	0.0	0.0	0.0	4.525913843859231E-5	6.034551791812308E-6
26-27	0.0	0.0	0.0	9.957010456490309E-5	6.034551791812308E-6
28-29	0.0	0.0	0.0	4.073322459473308E-4	6.034551791812308E-6
30-31	0.0	0.0	0.0	0.0012431176691133354	6.034551791812308E-6
32-33	0.0	0.0	0.0	0.0030896905174079017	6.034551791812308E-6
34-35	0.0	3.017275895906154E-6	0.0	0.005759979685284848	6.034551791812308E-6
36-37	0.0	6.034551791812308E-6	0.0	0.008484579819288105	6.034551791812308E-6
38-39	0.0	6.034551791812308E-6	0.0	0.014154041227695768	6.034551791812308E-6
40-41	0.0	6.034551791812308E-6	0.0	0.019745053462809872	6.034551791812308E-6
42-43	0.0	6.034551791812308E-6	0.0	0.024153293546728766	6.034551791812308E-6
44-45	0.0	6.034551791812308E-6	0.0	0.030758110482867335	6.034551791812308E-6
46-47	0.0	6.034551791812308E-6	0.0	0.035658166537818926	6.034551791812308E-6
48-49	0.0	6.034551791812308E-6	0.0	0.0421634133693926	6.034551791812308E-6
50-51	0.0	6.034551791812308E-6	0.0	0.048879869513679695	6.034551791812308E-6
52-53	0.0	6.034551791812308E-6	0.0	0.054793730269655755	6.034551791812308E-6
54-55	0.0	6.034551791812308E-6	0.0	0.05969680360050326	6.034551791812308E-6
56-57	0.0	6.034551791812308E-6	0.0	0.06738180530737622	6.034551791812308E-6
58-59	0.0	9.051827687718461E-6	0.0	0.07570345222828541	6.034551791812308E-6
60-61	0.0	1.2069103583624616E-5	0.0	0.08172291764061818	6.034551791812308E-6
62-63	0.0	1.2069103583624616E-5	0.0	0.08747686277411122	6.034551791812308E-6
64-65	0.0	1.2069103583624616E-5	0.0	0.09305580590564169	6.034551791812308E-6
66-67	0.0	1.2069103583624616E-5	0.0	0.09885802745346922	6.034551791812308E-6
68-69	0.0	1.2069103583624616E-5	0.0	0.10451843703418917	6.034551791812308E-6
70-71	0.0	1.2069103583624616E-5	0.0	0.11013057020057462	6.034551791812308E-6
72-73	0.0	1.2069103583624616E-5	0.0	0.11446941293888767	6.034551791812308E-6
74-75	0.0	1.2069103583624616E-5	0.0	0.11782462373513532	6.034551791812308E-6
76-77	0.0	1.2069103583624616E-5	0.0	0.12148759667276539	6.034551791812308E-6
78-79	0.0	1.508637947953077E-5	0.0	0.12381693366440494	6.034551791812308E-6
80-81	0.0	1.8103655375436923E-5	0.0	0.12466478819115456	6.034551791812308E-6
82-83	0.0	1.8103655375436923E-5	0.0	0.1252199669560013	6.034551791812308E-6
84-85	0.0	1.8103655375436923E-5	0.0	0.1254673835794656	6.034551791812308E-6
86-87	0.0	1.8103655375436923E-5	0.0	0.12557600551171821	6.034551791812308E-6
88	0.0	1.8103655375436923E-5	0.0	0.1256031609947814	6.034551791812308E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28885	0.0	53.36422	1
GTATCAA	54175	0.0	37.21036	1
CTTATAC	13635	0.0	29.114536	1
TATCAAC	70525	0.0	28.263147	2
TCAACGC	70210	0.0	28.234882	4
ATCAACG	70350	0.0	28.142977	3
TATACAC	15840	0.0	28.128279	3
CAACGCA	70420	0.0	28.122648	5
AACGCAG	72575	0.0	27.466467	6
ACGCAGA	81060	0.0	24.472872	7
CGCAGAG	82060	0.0	24.179888	8
TTATACA	17780	0.0	23.763811	2
GTACATG	66785	0.0	22.616423	1
TACATGG	69695	0.0	21.49787	2
GCAGAGT	92325	0.0	21.306864	9
ACATGGG	68955	0.0	21.127367	3
GAGTACT	55625	0.0	20.256062	12-13
TACACAT	22625	0.0	19.546017	5
CAGAGTA	90475	0.0	19.263695	10-11
ACACATC	21265	0.0	18.925219	6
>>END_MODULE