FastQCFastQC Report
Fri 27 May 2016
SRR2139245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20078975
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232950.050.6131061
GTATCAA423250.037.0499421
TCAACGC501800.030.5932834
ATCAACG510300.030.0836943
CAACGCA517450.029.6364675
TATCAAC534850.028.9704782
AACGCAG532950.028.8152376
ACGCAGA618550.024.8136487
CGCAGAG630250.024.3530048
GTACATG585550.021.8689821
TACATGG587000.021.4824162
ACATGGG598700.020.4249973
GCAGAGT752100.020.2588929
GAGTACT428050.019.1698812-13
AGAGTAC666250.018.54554210-11
CAGAGTA730750.018.2830310-11
CATGGGG440750.018.0600934
GTGGTAT124000.017.5263821
GTACTTT481900.016.99232314-15
TGGTATC116300.016.7099272
Produced by FastQC (version 0.11.5)