##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20078975
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31423018356266	33.0	33.0	33.0	33.0	33.0
2	32.35807684406201	33.0	33.0	33.0	33.0	33.0
3	32.386484569057934	33.0	33.0	33.0	33.0	33.0
4	36.252449788896094	37.0	37.0	37.0	33.0	37.0
5	36.29873731104302	37.0	37.0	37.0	33.0	37.0
6	36.281748396021214	37.0	37.0	37.0	33.0	37.0
7	36.24859336694229	37.0	37.0	37.0	33.0	37.0
8	36.23369400081428	37.0	37.0	37.0	33.0	37.0
9	36.29674487866039	37.0	37.0	37.0	33.0	37.0
10-11	36.2176677096316	37.0	37.0	37.0	33.0	37.0
12-13	36.177862341080655	37.0	37.0	37.0	33.0	37.0
14-15	37.94650986915418	40.0	37.0	40.0	33.0	40.0
16-17	37.878138400989094	40.0	37.0	40.0	33.0	40.0
18-19	37.84066430183812	40.0	37.0	40.0	33.0	40.0
20-21	37.846678769736876	40.0	37.0	40.0	33.0	40.0
22-23	37.85213438962688	40.0	37.0	40.0	33.0	40.0
24-25	37.84915219955092	40.0	37.0	40.0	33.0	40.0
26-27	37.718246615220586	40.0	37.0	40.0	33.0	40.0
28-29	37.64956614514436	40.0	37.0	40.0	33.0	40.0
30-31	37.534210414048154	40.0	37.0	40.0	33.0	40.0
32-33	37.485102789572224	40.0	37.0	40.0	33.0	40.0
34-35	37.45421497582255	40.0	37.0	40.0	33.0	40.0
36-37	37.40166338320271	40.0	37.0	40.0	33.0	40.0
38-39	37.346484238558546	40.0	37.0	40.0	33.0	40.0
40-41	37.317839681448554	40.0	37.0	40.0	33.0	40.0
42-43	37.22988067831862	40.0	37.0	40.0	33.0	40.0
44-45	37.166383061592185	40.0	37.0	40.0	33.0	40.0
46-47	37.117465612122444	40.0	37.0	40.0	33.0	40.0
48-49	37.10227961135645	40.0	37.0	40.0	33.0	40.0
50-51	37.019892299923754	37.0	37.0	40.0	33.0	40.0
52-53	36.89994290181167	37.0	37.0	40.0	33.0	40.0
54-55	36.700259550193536	37.0	37.0	40.0	33.0	40.0
56-57	36.59475324764246	37.0	37.0	40.0	33.0	40.0
58-59	36.449441824630654	37.0	37.0	40.0	33.0	40.0
60-61	36.30518913447982	37.0	37.0	40.0	33.0	40.0
62-63	36.1230105953495	37.0	37.0	40.0	33.0	40.0
64-65	35.93821413417963	37.0	35.0	40.0	33.0	40.0
66-67	35.73244408437441	37.0	33.0	40.0	33.0	40.0
68-69	35.512963921473414	37.0	33.0	37.0	27.0	40.0
70-71	35.27822129007727	37.0	33.0	37.0	27.0	40.0
72-73	35.055464056773246	37.0	33.0	37.0	27.0	40.0
74-75	34.857261313915544	37.0	33.0	37.0	27.0	40.0
76-77	32.87968592768523	33.0	33.0	37.0	27.0	37.0
78-79	34.307041940258564	37.0	33.0	37.0	27.0	37.0
80-81	34.24507960020247	37.0	33.0	37.0	27.0	37.0
82-83	33.93154628385366	37.0	33.0	37.0	27.0	37.0
84-85	34.030664430237486	37.0	33.0	37.0	27.0	37.0
86-87	33.97434855534944	37.0	33.0	37.0	27.0	37.0
88-89	33.85661256747326	37.0	33.0	37.0	27.0	37.0
90-91	33.5274283063746	37.0	33.0	37.0	27.0	37.0
92-93	33.6753798135464	35.0	33.0	37.0	27.0	37.0
94-95	33.58549084414575	33.0	33.0	37.0	27.0	37.0
96-97	33.6174800321093	33.0	33.0	37.0	27.0	37.0
98-99	33.77109734271258	33.0	33.0	37.0	27.0	37.0
100	33.81582110630332	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	36.0
20	297.0
21	1380.0
22	5288.0
23	14836.0
24	33472.0
25	64744.0
26	111378.0
27	174091.0
28	252480.0
29	348031.0
30	460655.0
31	601238.0
32	776038.0
33	1008335.0
34	1331019.0
35	1830940.0
36	2907653.0
37	5462990.0
38	4594706.0
39	99364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.836418442674486	20.13111227042217	13.971599645898259	24.06086964100508
2	14.739074081221776	21.379622216771523	41.22329949611472	22.658004205891984
3	18.052594816219454	26.444965442708106	30.424177528982433	25.078262212090006
4	12.47796762533944	18.22398304694338	39.0268527153403	30.271196612376876
5	13.478840428856554	37.78210790142425	35.515577861917755	13.223473807801444
6	29.801351911638918	38.0451043940241	18.926578672467095	13.22696502186989
7	26.38471336310743	32.64449505017064	23.412146287347834	17.558645299374096
8	24.386020700757882	36.73857853799808	21.247274823540543	17.628125937703494
9	25.717162071419104	16.667663563801508	21.07453823948829	36.540636125291094
10-11	24.11887962247643	26.6611453182205	29.12507819025777	20.094896869045304
12-13	25.10380225831341	24.26716871883937	29.08614493631319	21.542884086534027
14-15	22.258691425294113	25.599744460189207	26.603548047852005	25.53801606666467
16-17	21.87751615807082	28.665447314915227	27.254239820508765	22.202796706505186
18-19	21.927917137204464	27.881470543192567	28.853459900219008	21.337152419383955
20-21	22.677350856173113	27.117765306011403	28.72367587770068	21.481207960114805
22-23	22.4140848312937	26.98660732853016	28.57779989088777	22.021507949288367
24-25	22.201196194411022	27.14039968782539	28.618518731909077	22.039885385854515
26-27	22.140546085455483	27.29829004961276	28.54779241326239	22.013371451669368
28-29	22.124348089772532	27.35133415426748	28.572409712284486	21.951908043675505
30-31	22.395817453971162	27.210772725628324	28.414013175868273	21.97939664453224
32-33	22.203471999479525	27.363963418269073	28.364840220136383	22.067724362115023
34-35	22.332146048878272	27.36325109946145	28.32173393840163	21.982868913258653
36-37	22.31890688030515	27.320627113433755	28.296449744976382	22.064016261284717
38-39	22.176171176157677	27.47882161118834	28.344382898299315	22.000624314354667
40-41	22.421997777805082	27.304922956139183	28.201696879890886	22.07138238616485
42-43	22.30289153195266	27.435978874523425	27.99578795049643	22.265341643027483
44-45	22.410422485747613	27.48488036416249	27.871076533094246	22.23362061699565
46-47	22.48066834591803	27.504440821924554	27.589117560207043	22.425773271950373
48-49	22.34840644436407	27.670695058625743	27.583825802990532	22.397072694019656
50-51	22.387904757996633	27.726877049554243	27.519185067132373	22.366033125316747
52-53	22.606626516511316	27.60048435689789	27.380256684024403	22.41263244256639
54-55	22.530297251402267	27.506395577442667	27.441355182160493	22.521951988994573
56-57	22.50027879709055	27.65631378062894	27.408646603695168	22.434760818585342
58-59	22.497332567126303	27.658617866616837	27.399012441905214	22.445037124351646
60-61	22.55999995275256	27.61660077438553	27.439812131295483	22.38358714156643
62-63	22.538764552890424	27.721248747111098	27.355647359897805	22.384339340100674
64-65	22.51490366656937	27.64791027522574	27.3485564443755	22.488629613829396
66-67	22.53380104711525	27.685289298010012	27.238607114197904	22.542302540676836
68-69	22.48712928120151	27.67603738221628	27.325441200318696	22.51139213626351
70-71	22.517743837171057	27.46548435958233	27.355907091014668	22.66086471223194
72-73	22.54465674583328	27.52867835250062	27.253866527026844	22.67279837463926
74-75	22.37404769304249	27.668349886761508	27.292359892278228	22.66524252791778
76-77	22.609297301308732	27.530569379631437	27.195412147252963	22.66472117180687
78-79	22.485583998841893	27.615870148861926	27.1928120018799	22.705733850416284
80-81	22.525700129322562	27.671591816741998	27.138455961246912	22.66425209268853
82-83	22.577629445753384	27.58878965879864	27.199417111169954	22.63416378427802
84-85	22.557478290948996	27.63756321866501	27.222365690156973	22.58259280022902
86-87	22.457565979653673	27.604580392202273	27.30504329288836	22.632810335255694
88-89	22.48599408930835	27.61898098855416	27.237520105304437	22.657504816833058
90-91	22.56062637185297	27.630675028389994	27.24589718812838	22.562801411628662
92-93	22.512509842821515	27.6143015745407	27.26369043996372	22.60949814267406
94-95	22.431998541808742	27.631741481989618	27.285548701102158	22.650711275099482
96-97	22.465073515304987	27.739091489323144	27.38672680103068	22.409108194341194
98-99	23.57424613361422	29.687708991543737	27.5645273446358	19.173517530206244
100	22.49436637275019	30.711691894518495	21.393258158589497	25.40068357414182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4229.0
1	3830.0
2	5157.0
3	7267.0
4	9550.5
5	15581.0
6	20956.5
7	24272.0
8	25993.5
9	25220.0
10	23055.5
11	20791.5
12	22335.5
13	29076.0
14	44645.0
15	67227.0
16	86836.5
17	99054.0
18	102821.0
19	98554.0
20	89562.0
21	79915.0
22	74011.5
23	75971.0
24	87611.5
25	109250.5
26	140307.0
27	176982.5
28	216645.0
29	260721.0
30	303973.5
31	347094.5
32	393745.5
33	438236.0
34	482762.5
35	527443.0
36	575683.0
37	619669.5
38	650786.0
39	679034.5
40	711479.0
41	740890.0
42	762595.0
43	782615.5
44	799293.5
45	816088.0
46	823660.0
47	823643.5
48	825965.5
49	819846.5
50	806060.5
51	787828.5
52	758790.0
53	719093.5
54	677899.0
55	632312.5
56	580262.0
57	523425.0
58	463309.0
59	401479.0
60	336592.0
61	274895.5
62	218882.5
63	167575.5
64	122762.0
65	88869.5
66	65144.0
67	48437.0
68	36093.5
69	25459.0
70	17677.5
71	12546.5
72	8568.0
73	5669.0
74	3770.5
75	2473.0
76	1622.5
77	1065.5
78	733.0
79	542.0
80	388.5
81	268.0
82	192.5
83	142.5
84	104.5
85	75.5
86	58.0
87	43.0
88	32.0
89	24.5
90	17.0
91	12.5
92	11.0
93	7.0
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.9882003438920565E-5
10-11	0.002721752479895015
12-13	2.6146753009055493E-4
14-15	0.003458841898055055
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	389.0
20-21	1410.0
22-23	2861.0
24-25	6262.0
26-27	8286.0
28-29	13365.0
30-31	26277.0
32-33	33312.0
34-35	47260.0
36-37	65102.0
38-39	77885.0
40-41	86868.0
42-43	107713.0
44-45	129719.0
46-47	127568.0
48-49	115285.0
50-51	104309.0
52-53	104627.0
54-55	114582.0
56-57	122554.0
58-59	126239.0
60-61	126738.0
62-63	125660.0
64-65	136024.0
66-67	147482.0
68-69	153408.0
70-71	154957.0
72-73	152050.0
74-75	159750.0
76-77	154451.0
78-79	176779.0
80-81	181374.0
82-83	176204.0
84-85	182667.0
86-87	196639.0
88-89	216131.0
90-91	227110.0
92-93	247766.0
94-95	308254.0
96-97	748451.0
98-99	1287977.0
100-101	1.339723E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.93639229907645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14439820356101	69.66008801466785
2	8.356975608124042	13.360529612896327
3	2.3017851374849947	5.519891992145524
4	0.9356136587038643	2.9915832185010522
5	0.4562164440174315	1.8234148321133525
6	0.25160704440124987	1.2067535643881673
7	0.15202338728349987	0.8506540787169794
8	0.10104419204804739	0.646168654007675
9	0.06323458940080606	0.45492704506924686
>10	0.22911231273205523	2.861789713853505
>50	0.006001284021346037	0.3215436133650752
>100	0.0019189069910023932	0.24414530719907937
>500	2.7705261479019853E-5	0.014173766811957452
>1k	4.1525969154519454E-5	0.04433658626409163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.494100171946028E-5
2	0.0	0.0	0.0	4.9803339064867606E-6	1.494100171946028E-5
3	0.0	9.960667812973521E-6	0.0	4.9803339064867606E-6	1.494100171946028E-5
4	0.0	4.4823005158380844E-5	0.0	1.494100171946028E-5	1.494100171946028E-5
5	0.0	5.976400687784112E-5	0.0	1.494100171946028E-5	1.494100171946028E-5
6	0.0	5.976400687784112E-5	0.0	1.494100171946028E-5	2.4901669532433802E-5
7	0.0	5.976400687784112E-5	0.0	1.494100171946028E-5	3.4862337345407325E-5
8	0.0	7.968534250378817E-5	0.0	1.494100171946028E-5	4.4823005158380844E-5
9	0.0	1.5937068500757634E-4	0.0	1.494100171946028E-5	4.9803339064867604E-5
10-11	9.960667812973521E-6	1.5937068500757634E-4	0.0	1.9921335625947042E-5	4.9803339064867604E-5
12-13	9.960667812973521E-6	1.5937068500757634E-4	0.0	2.988200343892056E-5	4.9803339064867604E-5
14-15	1.7431168672703662E-5	2.1664452493217406E-4	0.0	3.4862337345407325E-5	6.474434078432788E-5
16-17	2.4901669532433802E-5	2.1913469188541745E-4	0.0	5.229350601811098E-5	7.221484164405802E-5
18-19	2.4901669532433802E-5	2.3656586055812112E-4	0.0	8.217550945703155E-5	9.462634422324845E-5
20-21	2.988200343892056E-5	2.639576970437983E-4	0.0	1.145476798491955E-4	1.1952801375568225E-4
22-23	3.4862337345407325E-5	3.1376103610866594E-4	0.0	1.2948868156865577E-4	1.2699851461541237E-4
24-25	4.731317211162423E-5	3.6356437517353355E-4	0.0	1.5190018414784617E-4	1.2948868156865577E-4
26-27	5.4783672971354364E-5	3.73525042986507E-4	0.0	1.7929202063352338E-4	1.2948868156865577E-4
28-29	5.4783672971354364E-5	3.760152099397504E-4	0.0	3.6107420822029015E-4	1.3197884852189916E-4
30-31	5.976400687784112E-5	4.2083821509813124E-4	0.0	0.0012973769826398012	1.369591824283859E-4
32-33	6.474434078432788E-5	4.3328904986434817E-4	0.0	0.0037576619324442605	1.394493493816293E-4
34-35	6.723450773757127E-5	5.030137245551628E-4	0.0	0.006935114964782814	1.394493493816293E-4
36-37	6.972467469081465E-5	5.926597348719245E-4	0.0	0.010354114191585974	1.394493493816293E-4
38-39	6.972467469081465E-5	6.100909035446282E-4	0.0	0.015297095593774086	1.5190018414784617E-4
40-41	6.972467469081465E-5	7.047172477678766E-4	0.0	0.020185293322990842	1.5688051805433297E-4
42-43	6.972467469081465E-5	7.296189173003104E-4	0.0	0.02413469811083484	1.5937068500757634E-4
44-45	7.221484164405802E-5	8.342059293365324E-4	0.0	0.03007125612736706	1.5937068500757634E-4
46-47	7.47050085973014E-5	9.313224405130242E-4	0.0	0.035726425278182775	1.743116867270366E-4
48-49	7.47050085973014E-5	9.387929413727544E-4	0.0	0.040704269017716295	1.8178218758676677E-4
50-51	7.47050085973014E-5	0.0010259487847362725	0.0	0.045293646712543836	1.8925268844649688E-4
52-53	7.719517555054479E-5	0.0010458701203622197	0.0	0.04969128155197165	1.9672318930622705E-4
54-55	7.968534250378817E-5	0.0010583209551284366	0.0	0.05335929747409915	2.0917402407244392E-4
56-57	7.968534250378817E-5	0.0010857127916141138	0.0	0.058606079244582956	2.2162485883866085E-4
58-59	7.968534250378817E-5	0.001100653793333574	0.0	0.06423385655891299	2.29095359698391E-4
60-61	8.466567641027492E-5	0.0011305357967724946	0.0	0.0686489225670135	2.3656586055812112E-4
62-63	8.466567641027492E-5	0.0011554374663049283	0.0	0.07267552253040804	2.515068622775814E-4
64-65	8.466567641027492E-5	0.0012849261478735843	0.0	0.07679923900497909	2.5648719618406814E-4
66-67	8.466567641027492E-5	0.0012923966487333144	0.0	0.08087066197353202	2.5897736313731153E-4
68-69	8.964601031676169E-5	0.0013496704886579122	0.0	0.08523094430866118	2.6146753009055493E-4
70-71	8.964601031676169E-5	0.001374572158190346	0.0	0.08931481811198032	2.639576970437983E-4
72-73	8.964601031676169E-5	0.0013870229929565628	0.0	0.09255203515119671	2.764085318100152E-4
74-75	8.964601031676169E-5	0.0014169049963954833	0.0	0.09527129746413848	2.8387903266974534E-4
76-77	8.964601031676169E-5	0.001479159170226568	0.0	0.09803787294919189	2.8387903266974534E-4
78-79	8.964601031676169E-5	0.0015090411736654885	0.0	0.1000424573465528	2.8387903266974534E-4
80-81	9.213617727000506E-5	0.0015165116745252186	0.0	0.10065254825009742	2.988200343892056E-4
82-83	9.462634422324844E-5	0.0015264723423381922	0.0	0.10128754082317448	3.287020378281262E-4
84-85	9.462634422324844E-5	0.0015364330101511657	0.0	0.10154900835326505	3.287020378281262E-4
86-87	9.462634422324844E-5	0.001558844512730356	0.0	0.10164861503139477	3.311922047813696E-4
88	9.462634422324844E-5	0.001663431524766578	0.0	0.10166853636702072	3.486233734540732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23295	0.0	50.613106	1
GTATCAA	42325	0.0	37.049942	1
TCAACGC	50180	0.0	30.593283	4
ATCAACG	51030	0.0	30.083694	3
CAACGCA	51745	0.0	29.636467	5
TATCAAC	53485	0.0	28.970478	2
AACGCAG	53295	0.0	28.815237	6
ACGCAGA	61855	0.0	24.813648	7
CGCAGAG	63025	0.0	24.353004	8
GTACATG	58555	0.0	21.868982	1
TACATGG	58700	0.0	21.482416	2
ACATGGG	59870	0.0	20.424997	3
GCAGAGT	75210	0.0	20.258892	9
GAGTACT	42805	0.0	19.16988	12-13
AGAGTAC	66625	0.0	18.545542	10-11
CAGAGTA	73075	0.0	18.28303	10-11
CATGGGG	44075	0.0	18.060093	4
GTGGTAT	12400	0.0	17.526382	1
GTACTTT	48190	0.0	16.992323	14-15
TGGTATC	11630	0.0	16.709927	2
>>END_MODULE