FastQCFastQC Report
Fri 27 May 2016
SRR2139244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9194113
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123900.051.6573221
GTATCAA226500.037.22051
TCAACGC278400.029.7325764
ATCAACG282200.029.3924063
CAACGCA285100.029.0347945
TATCAAC289200.028.886572
AACGCAG291650.028.4118426
ACGCAGA330050.025.093377
CGCAGAG359800.023.0423898
GTACATG281950.022.2182481
GTGGTAT56150.021.9350281
TACATGG282900.021.513112
ACATGGG285400.020.9377543
GCAGAGT393150.020.7972019
TGGTATC55350.020.4105132
CTTATAC57100.020.231251
GAGTACT236700.019.70254712-13
CAGAGTA379750.019.03121810-11
AGAGTAC352250.018.83486210-11
CATGGGG217000.018.7693334
Produced by FastQC (version 0.11.5)