##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9194113
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29307938677717	33.0	33.0	33.0	33.0	33.0
2	32.33699487922326	33.0	33.0	33.0	33.0	33.0
3	32.362324239434514	33.0	33.0	33.0	33.0	33.0
4	36.22742857304451	37.0	37.0	37.0	33.0	37.0
5	36.272372332165155	37.0	37.0	37.0	33.0	37.0
6	36.26117462337041	37.0	37.0	37.0	33.0	37.0
7	36.224518450012525	37.0	37.0	37.0	33.0	37.0
8	36.20962663826298	37.0	37.0	37.0	33.0	37.0
9	36.274751572011354	37.0	37.0	37.0	33.0	37.0
10-11	36.19409751653041	37.0	37.0	37.0	33.0	37.0
12-13	36.15276808105361	37.0	37.0	37.0	33.0	37.0
14-15	37.88946019045013	40.0	37.0	40.0	33.0	40.0
16-17	37.821403598150255	40.0	37.0	40.0	33.0	40.0
18-19	37.790681983134206	40.0	37.0	40.0	33.0	40.0
20-21	37.79929606359713	40.0	37.0	40.0	33.0	40.0
22-23	37.80269632951779	40.0	37.0	40.0	33.0	40.0
24-25	37.79616013652452	40.0	37.0	40.0	33.0	40.0
26-27	37.65820842613553	40.0	37.0	40.0	33.0	40.0
28-29	37.58566585592446	40.0	37.0	40.0	33.0	40.0
30-31	37.46556580084558	40.0	37.0	40.0	33.0	40.0
32-33	37.408381183312166	40.0	37.0	40.0	33.0	40.0
34-35	37.369097249672706	40.0	37.0	40.0	33.0	40.0
36-37	37.31041747725912	40.0	37.0	40.0	33.0	40.0
38-39	37.24433360418545	40.0	37.0	40.0	33.0	40.0
40-41	37.21370237411129	40.0	37.0	40.0	33.0	40.0
42-43	37.124282483234396	40.0	37.0	40.0	33.0	40.0
44-45	37.05575371673707	38.5	37.0	40.0	33.0	40.0
46-47	37.00354057508552	37.0	37.0	40.0	33.0	40.0
48-49	36.980178897723235	37.0	37.0	40.0	33.0	40.0
50-51	36.88472957003832	37.0	37.0	40.0	33.0	40.0
52-53	36.76699206138408	37.0	37.0	40.0	33.0	40.0
54-55	36.56759714288057	37.0	37.0	40.0	33.0	40.0
56-57	36.4592472489484	37.0	37.0	40.0	33.0	40.0
58-59	36.3065146015755	37.0	37.0	40.0	33.0	40.0
60-61	36.15823012991379	37.0	37.0	40.0	33.0	40.0
62-63	35.98279476806934	37.0	35.0	40.0	33.0	40.0
64-65	35.7994413324282	37.0	33.0	40.0	33.0	40.0
66-67	35.58656533314692	37.0	33.0	40.0	27.0	40.0
68-69	35.37576983461864	37.0	33.0	37.0	27.0	40.0
70-71	35.14659233206552	37.0	33.0	37.0	27.0	40.0
72-73	34.938927845167996	37.0	33.0	37.0	27.0	40.0
74-75	34.75228943591982	37.0	33.0	37.0	27.0	40.0
76-77	32.76374593648275	33.0	33.0	37.0	27.0	37.0
78-79	34.22276820902106	37.0	33.0	37.0	27.0	37.0
80-81	34.17524919761722	37.0	33.0	37.0	27.0	37.0
82-83	33.86299474910693	37.0	33.0	37.0	27.0	37.0
84-85	33.962094251190436	37.0	33.0	37.0	27.0	37.0
86-87	33.909668373019116	37.0	33.0	37.0	27.0	37.0
88-89	33.79735761412333	37.0	33.0	37.0	27.0	37.0
90-91	33.4713307355963	35.0	33.0	37.0	27.0	37.0
92-93	33.613206049245136	33.0	33.0	37.0	27.0	37.0
94-95	33.52234103765876	33.0	33.0	37.0	27.0	37.0
96-97	33.566812084984576	33.0	33.0	37.0	27.0	37.0
98-99	33.723458179483586	33.0	33.0	37.0	27.0	37.0
100	33.76649772680649	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	3.0
19	19.0
20	123.0
21	688.0
22	2466.0
23	7099.0
24	16145.0
25	30973.0
26	53457.0
27	83230.0
28	120359.0
29	165067.0
30	218461.0
31	283942.0
32	366259.0
33	475193.0
34	624435.0
35	857155.0
36	1362198.0
37	2470700.0
38	2010401.0
39	45738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70496925587058	20.168438216932945	14.144812011773187	23.981780515423292
2	14.841301167388307	21.389143248511303	41.039782739237594	22.729772844862794
3	17.95446716828475	26.438733132820968	30.557825425900248	25.048974272994034
4	12.435163674842805	18.410867910803358	38.65069963790961	30.50326877644423
5	13.55784946302052	37.49826655382634	35.52474284360003	13.419141139553103
6	29.79010590798699	37.97718170311807	18.72102289802181	13.511689490873128
7	26.170224359870275	32.63006447712792	23.369236379844363	17.83047478315744
8	24.505615713011142	36.409711301133676	21.045499440783466	18.039173545071723
9	25.625498144899694	16.448701081735788	21.16219597933982	36.763604794024694
10-11	24.275025551024086	26.66371252070064	28.758757816427128	20.302504111848144
12-13	25.14385480351785	24.375517962401783	28.691610726228767	21.789016507851603
14-15	22.25228779326576	25.468185386794612	26.55220467197764	25.727322147961985
16-17	21.852461460937015	28.663134768954873	27.047606441208632	22.436797328899484
18-19	21.92875484562785	27.657953518735308	28.806938744390028	21.60635289124682
20-21	22.798338390761188	26.77186363152833	28.766541965846752	21.663256011863727
22-23	22.55620093722755	26.781954238619416	28.5398718762277	22.121972947925332
24-25	22.321698063731034	26.949314960282077	28.749950220672034	21.979036755314855
26-27	22.196874266100746	27.00812932464805	28.717902665263566	22.07709374398764
28-29	22.18379676970594	26.983958759311243	28.722885366866375	22.109359104116443
30-31	22.530281175980008	26.712086181255014	28.550204535593203	22.207428107171776
32-33	22.402260117361916	26.866828137455496	28.52710055724092	22.203811187941668
34-35	22.532623366775965	27.04934649111122	28.403601449973703	22.014428692139113
36-37	22.563859613910676	26.918059194227062	28.248850064812103	22.26923112705016
38-39	22.218427352867135	27.19022539586242	28.38450750645028	22.206839744820165
40-41	22.469766155052266	26.938582316048958	28.35043660617019	22.24121492272858
42-43	22.35391826519201	27.10767631555254	28.076689948118105	22.461715471137346
44-45	22.34057985363632	27.200668735484562	27.93359037971389	22.525161031165222
46-47	22.589221018502677	27.121613057178852	27.657423277278546	22.631742647039925
48-49	22.64113324608051	27.348341541193182	27.432387612804515	22.578137599921792
50-51	22.58111828787023	27.383036656443405	27.471160230972675	22.564684824713694
52-53	22.816297374501023	27.31092360729362	27.38345657186064	22.489322446344715
54-55	22.799223468344504	27.23367261870781	27.31410821055194	22.652995702395742
56-57	22.82340330666145	27.511955790997916	27.052964482621476	22.611676419719153
58-59	22.622815208092035	27.497036096661027	27.349014818437382	22.53113387680955
60-61	22.742257216323917	27.522148957328458	27.168407914930874	22.56718591141675
62-63	22.734833060349644	27.590673764730507	27.225000036592988	22.44949313832686
64-65	22.80464067790751	27.428722503358433	27.171114156255005	22.595522662479055
66-67	22.832812223262483	27.499533621597827	26.985834268367174	22.681819886772516
68-69	22.838128198795122	27.39065601780587	27.085589494404843	22.68562628899416
70-71	22.81906606052782	27.23603814235239	27.10893157866846	22.83596421845133
72-73	22.80798618346285	27.307692799370027	26.98201088908803	22.902310128079094
74-75	22.657944619642567	27.370666975676627	27.058581539756116	22.912806864924686
76-77	22.84429601320609	27.34471143997761	26.92377909095032	22.887213455865986
78-79	22.61574633930153	27.418058489591296	27.049258201783115	22.91693696932406
80-81	22.772019308791254	27.289952103203024	27.047867128396703	22.890161459609022
82-83	22.908425095166127	27.157348303353007	27.20731981017822	22.726906791302646
84-85	22.80870198782222	27.187636314925	27.204037454709024	22.799624242543754
86-87	22.68361142922556	27.32251466737497	27.146925122589003	22.846948780810465
88-89	22.87918319810833	27.14657488545403	27.04512391292702	22.929118003510624
90-91	22.9112257704959	27.138620162824868	27.09757763601263	22.852576430666602
92-93	22.815827371354132	27.170418352306534	27.273059101652137	22.740695174687197
94-95	22.63302973812461	27.266701656213193	27.279675062480553	22.820593543181644
96-97	22.67767701568754	27.344550025688473	27.35315885460203	22.624614104021955
98-99	23.853366150169432	29.30067341765613	27.499061279617816	19.34689915255662
100	22.700302529732443	30.35900615546798	21.385634262852143	25.55505705194743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2633.0
1	2237.0
2	2799.5
3	3932.5
4	5138.5
5	8328.5
6	11251.5
7	13416.5
8	15069.5
9	15122.0
10	13677.0
11	11983.0
12	12794.0
13	16932.5
14	26260.0
15	39351.0
16	50536.0
17	57269.0
18	58965.0
19	56134.5
20	51052.0
21	46061.0
22	43236.5
23	44680.5
24	50831.5
25	62100.0
26	77935.5
27	94274.5
28	111233.0
29	130102.0
30	147158.5
31	162599.0
32	178856.0
33	196013.5
34	213867.5
35	231145.0
36	250987.5
37	268719.0
38	280806.0
39	293252.0
40	306825.0
41	318537.5
42	326547.0
43	332873.0
44	340492.0
45	353225.5
46	366795.0
47	381286.0
48	392057.5
49	390773.0
50	384992.5
51	379521.5
52	361585.0
53	335240.5
54	316023.5
55	298812.0
56	280165.0
57	258026.5
58	232855.5
59	205419.0
60	175412.5
61	149974.0
62	124372.5
63	100843.0
64	83167.0
65	65666.5
66	52253.5
67	38808.0
68	25554.5
69	18522.0
70	13473.0
71	8726.0
72	5996.5
73	4349.5
74	3287.0
75	2350.5
76	1505.0
77	880.5
78	660.5
79	553.0
80	380.5
81	240.0
82	187.5
83	117.0
84	61.5
85	41.0
86	33.0
87	29.0
88	23.5
89	18.0
90	12.5
91	8.0
92	5.5
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0876525011167472E-5
10-11	0.0027680756153421215
12-13	3.643635878741103E-4
14-15	0.0034696114785624237
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	232.0
20-21	793.0
22-23	1685.0
24-25	3467.0
26-27	5172.0
28-29	8929.0
30-31	19861.0
32-33	25172.0
34-35	34750.0
36-37	48792.0
38-39	56321.0
40-41	59059.0
42-43	71662.0
44-45	84326.0
46-47	81283.0
48-49	72050.0
50-51	63396.0
52-53	62555.0
54-55	67274.0
56-57	71174.0
58-59	71056.0
60-61	69890.0
62-63	68722.0
64-65	74843.0
66-67	78656.0
68-69	76421.0
70-71	76116.0
72-73	72310.0
74-75	76492.0
76-77	72356.0
78-79	82205.0
80-81	82630.0
82-83	80817.0
84-85	83652.0
86-87	88865.0
88-89	98298.0
90-91	103514.0
92-93	111443.0
94-95	132743.0
96-97	326755.0
98-99	564159.0
100-101	5864217.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.13449520712895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72570556217563	75.49026944577129
2	7.076786603852264	11.908037372073652
3	1.7176895235821925	4.335508229674847
4	0.6397531377171994	2.153012291960537
5	0.30195517545715306	1.2702423131133809
6	0.16160856172485366	0.815811285712242
7	0.1006504433462793	0.5927721970319058
8	0.055672324395277244	0.37471703280033447
9	0.044740842000082943	0.33878233411370023
>10	0.17003918974478674	2.3141828026446225
>50	0.004307027904266573	0.24427388393476948
>100	0.0010440784026408507	0.13957720584720315
>500	4.752969731326542E-5	0.0228136053215914
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.525915006700484E-5	0.0	0.0	0.0
3	0.0	6.525915006700484E-5	0.0	0.0	0.0
4	0.0	1.3051830013400967E-4	0.0	0.0	0.0
5	0.0	1.3051830013400967E-4	0.0	0.0	0.0
6	6.525915006700484E-5	1.3051830013400967E-4	0.0	1.0876525011167472E-5	0.0
7	6.525915006700484E-5	1.4139482514517713E-4	0.0	1.0876525011167472E-5	0.0
8	6.525915006700484E-5	1.522713501563446E-4	0.0	2.1753050022334944E-5	0.0
9	6.525915006700484E-5	3.045427003126892E-4	0.0	2.1753050022334944E-5	1.0876525011167472E-5
10-11	7.069741257258858E-5	3.045427003126892E-4	0.0	2.1753050022334944E-5	1.0876525011167472E-5
12-13	7.61356750781723E-5	3.045427003126892E-4	0.0	2.1753050022334944E-5	2.1753050022334944E-5
14-15	7.61356750781723E-5	4.078696879187802E-4	0.0	2.1753050022334944E-5	2.1753050022334944E-5
16-17	9.788872510050725E-5	4.1330795042436396E-4	0.0	2.1753050022334944E-5	2.1753050022334944E-5
18-19	9.788872510050725E-5	4.350610004466989E-4	0.0	2.1753050022334944E-5	2.1753050022334944E-5
20-21	9.788872510050725E-5	4.894436255025363E-4	0.0	4.350610004466989E-5	2.1753050022334944E-5
22-23	9.788872510050725E-5	5.166349380304549E-4	0.0	6.525915006700484E-5	2.7191312527918683E-5
24-25	9.788872510050725E-5	5.601410380751248E-4	0.0	1.0332698760609098E-4	3.262957503350242E-5
26-27	9.788872510050725E-5	5.76455825591876E-4	0.0	1.957774502010145E-4	3.262957503350242E-5
28-29	9.788872510050725E-5	5.76455825591876E-4	0.0	6.145236631309622E-4	3.262957503350242E-5
30-31	9.788872510050725E-5	6.417149756588809E-4	0.0	0.0025233538025908535	3.262957503350242E-5
32-33	9.788872510050725E-5	6.417149756588809E-4	0.0	0.006830457707013172	3.262957503350242E-5
34-35	9.788872510050725E-5	7.559184882761393E-4	0.0	0.01168682612449945	3.262957503350242E-5
36-37	9.788872510050725E-5	8.701220008933977E-4	0.0	0.01850096904399587	3.262957503350242E-5
38-39	9.788872510050725E-5	9.027515759269002E-4	0.0	0.02580999385150041	4.350610004466989E-5
40-41	9.788872510050725E-5	0.0010223933510497424	0.0	0.03234678538321206	5.9820887561421094E-5
42-43	9.788872510050725E-5	0.0010604611885888285	0.0	0.039101107415147066	6.525915006700484E-5
44-45	9.788872510050725E-5	0.0012127325387451733	0.0	0.04621979303495617	7.069741257258858E-5
46-47	1.0876525011167472E-4	0.0013269360513624316	0.0	0.05234327661624346	7.61356750781723E-5
48-49	1.0876525011167472E-4	0.0013269360513624316	0.0	0.058896382935471864	9.788872510050725E-5
50-51	1.2508003762842594E-4	0.0014792074015187763	0.0	0.06525915006700483	9.788872510050725E-5
52-53	1.3051830013400967E-4	0.0015009604515411111	0.0	0.07134456581075303	1.0332698760609098E-4
54-55	1.3051830013400967E-4	0.001506398714046695	0.0	0.07676651352882002	1.0876525011167472E-4
56-57	1.359565626395934E-4	0.0015118369765522786	0.0	0.08441271061167074	1.0876525011167472E-4
58-59	1.4139482514517713E-4	0.0015118369765522786	0.0	0.09276588182024736	1.0876525011167472E-4
60-61	1.4683308765076086E-4	0.0015553430765969487	0.0	0.09859026096372756	1.0876525011167472E-4
62-63	1.522713501563446E-4	0.0015988491766416185	0.0	0.10432218964461282	1.0876525011167472E-4
64-65	1.522713501563446E-4	0.0018816388269319727	0.0	0.11001605048795898	1.0876525011167472E-4
66-67	1.6314787516751207E-4	0.0019033918769543075	0.0	0.1151878381307691	1.0876525011167472E-4
68-69	1.6314787516751207E-4	0.00193602145198781	0.0	0.12034331098606249	1.0876525011167472E-4
70-71	1.6858613767309583E-4	0.0019632127645157287	0.0	0.12520511766605436	1.0876525011167472E-4
72-73	1.7402440017867955E-4	0.00197952755203248	0.0	0.12875630308220054	1.3051830013400967E-4
74-75	1.7402440017867955E-4	0.0020447867020994847	0.0	0.13184523618537208	1.3051830013400967E-4
76-77	1.7402440017867955E-4	0.002158990214716743	0.0	0.1345861204881863	1.3051830013400967E-4
78-79	1.7402440017867955E-4	0.0021970580522558294	0.0	0.13660371587775788	1.4139482514517713E-4
80-81	1.7402440017867955E-4	0.0021970580522558294	0.0	0.1372236778033944	1.4683308765076086E-4
82-83	1.7946266268426328E-4	0.002202496314761413	0.0	0.13775662752894163	1.522713501563446E-4
84-85	1.957774502010145E-4	0.0022188111022781645	0.0	0.13801222586670406	1.522713501563446E-4
86-87	2.0121571270659825E-4	0.002240564152300499	0.0	0.13818625026688272	1.522713501563446E-4
88	2.1753050022334944E-4	0.002349329402412174	0.0	0.1381971267918939	1.6314787516751207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12390	0.0	51.657322	1
GTATCAA	22650	0.0	37.2205	1
TCAACGC	27840	0.0	29.732576	4
ATCAACG	28220	0.0	29.392406	3
CAACGCA	28510	0.0	29.034794	5
TATCAAC	28920	0.0	28.88657	2
AACGCAG	29165	0.0	28.411842	6
ACGCAGA	33005	0.0	25.09337	7
CGCAGAG	35980	0.0	23.042389	8
GTACATG	28195	0.0	22.218248	1
GTGGTAT	5615	0.0	21.935028	1
TACATGG	28290	0.0	21.51311	2
ACATGGG	28540	0.0	20.937754	3
GCAGAGT	39315	0.0	20.797201	9
TGGTATC	5535	0.0	20.410513	2
CTTATAC	5710	0.0	20.23125	1
GAGTACT	23670	0.0	19.702547	12-13
CAGAGTA	37975	0.0	19.031218	10-11
AGAGTAC	35225	0.0	18.834862	10-11
CATGGGG	21700	0.0	18.769333	4
>>END_MODULE