FastQCFastQC Report
Fri 27 May 2016
SRR2139243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8742546
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110100.054.9063341
GTATCAA203350.038.4727671
TCAACGC254100.030.4329724
ATCAACG257250.030.010113
CAACGCA261950.029.4743585
TATCAAC267000.029.0109942
AACGCAG269650.028.6965776
ACGCAGA311200.024.8928157
CGCAGAG341550.022.6809798
GTACATG278000.021.9466251
TACATGG278100.021.4123292
GTGGTAT47800.020.9879841
GCAGAGT368500.020.7781319
ACATGGG282050.020.6850173
GAGTACT215700.019.53364212-13
AGAGTAC333750.019.01903510-11
CAGAGTA357700.018.9796110-11
TGGTATC47700.018.9558682
CATGGGG208800.018.9301874
TATATAC107500.018.6646613
Produced by FastQC (version 0.11.5)