##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8742546
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30488784388438	33.0	33.0	33.0	33.0	33.0
2	32.35117275905669	33.0	33.0	33.0	33.0	33.0
3	32.37976809043956	33.0	33.0	33.0	33.0	33.0
4	36.246844454693175	37.0	37.0	37.0	33.0	37.0
5	36.29456647983322	37.0	37.0	37.0	33.0	37.0
6	36.27827580203753	37.0	37.0	37.0	33.0	37.0
7	36.241753489200974	37.0	37.0	37.0	33.0	37.0
8	36.22716586220993	37.0	37.0	37.0	33.0	37.0
9	36.290709136674835	37.0	37.0	37.0	33.0	37.0
10-11	36.21192585089057	37.0	37.0	37.0	33.0	37.0
12-13	36.1704895804952	37.0	37.0	37.0	33.0	37.0
14-15	37.92543430712289	40.0	37.0	40.0	33.0	40.0
16-17	37.85741127355807	40.0	37.0	40.0	33.0	40.0
18-19	37.82454842102061	40.0	37.0	40.0	33.0	40.0
20-21	37.83355968704198	40.0	37.0	40.0	33.0	40.0
22-23	37.837505032821625	40.0	37.0	40.0	33.0	40.0
24-25	37.83312175604759	40.0	37.0	40.0	33.0	40.0
26-27	37.69902399796554	40.0	37.0	40.0	33.0	40.0
28-29	37.627439933363185	40.0	37.0	40.0	33.0	40.0
30-31	37.50465461856686	40.0	37.0	40.0	33.0	40.0
32-33	37.449967361548914	40.0	37.0	40.0	33.0	40.0
34-35	37.41747217915495	40.0	37.0	40.0	33.0	40.0
36-37	37.3566767451088	40.0	37.0	40.0	33.0	40.0
38-39	37.29726201311841	40.0	37.0	40.0	33.0	40.0
40-41	37.26133816685327	40.0	37.0	40.0	33.0	40.0
42-43	37.17110642446788	40.0	37.0	40.0	33.0	40.0
44-45	37.10449679883638	40.0	37.0	40.0	33.0	40.0
46-47	37.04775952893526	37.0	37.0	40.0	33.0	40.0
48-49	37.02257355355559	37.0	37.0	40.0	33.0	40.0
50-51	36.9288601906138	37.0	37.0	40.0	33.0	40.0
52-53	36.80905882507042	37.0	37.0	40.0	33.0	40.0
54-55	36.61008944492555	37.0	37.0	40.0	33.0	40.0
56-57	36.50120903901211	37.0	37.0	40.0	33.0	40.0
58-59	36.34505973834477	37.0	37.0	40.0	33.0	40.0
60-61	36.194728505954686	37.0	37.0	40.0	33.0	40.0
62-63	36.01571920067184	37.0	35.0	40.0	33.0	40.0
64-65	35.82929376148186	37.0	33.0	40.0	33.0	40.0
66-67	35.61781980607343	37.0	33.0	40.0	27.0	40.0
68-69	35.40443840659792	37.0	33.0	37.0	27.0	40.0
70-71	35.17557328890112	37.0	33.0	37.0	27.0	40.0
72-73	34.96449749502787	37.0	33.0	37.0	27.0	40.0
74-75	34.7744351643656	37.0	33.0	37.0	27.0	40.0
76-77	32.79635740821937	33.0	33.0	37.0	27.0	37.0
78-79	34.23836677181272	37.0	33.0	37.0	27.0	37.0
80-81	34.17953687710886	37.0	33.0	37.0	27.0	37.0
82-83	33.85502219112199	37.0	33.0	37.0	27.0	37.0
84-85	33.96376947200724	37.0	33.0	37.0	27.0	37.0
86-87	33.91727819822161	37.0	33.0	37.0	27.0	37.0
88-89	33.80446611381771	37.0	33.0	37.0	27.0	37.0
90-91	33.46841364029089	35.0	33.0	37.0	27.0	37.0
92-93	33.612017003974614	33.0	33.0	37.0	27.0	37.0
94-95	33.521095032023226	33.0	33.0	37.0	27.0	37.0
96-97	33.56484669264803	33.0	33.0	37.0	27.0	37.0
98-99	33.72921617997759	33.0	33.0	37.0	27.0	37.0
100	33.76385400426158	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	22.0
20	107.0
21	576.0
22	2208.0
23	6453.0
24	14597.0
25	28624.0
26	49701.0
27	77661.0
28	112550.0
29	155095.0
30	206032.0
31	268624.0
32	346930.0
33	449492.0
34	592898.0
35	815647.0
36	1293663.0
37	2366909.0
38	1916704.0
39	38051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.912310212608546	20.028707884408043	14.049397052071559	24.00958485091185
2	14.907808320368002	21.29067436419551	41.07289798646756	22.72861932896893
3	18.04593307258549	26.34520882132047	30.311364675690584	25.297493430403456
4	12.433460458772535	18.323804072635134	38.60249634374243	30.6402391248499
5	13.624475067102878	37.53286514020058	35.395524370131994	13.44713542256455
6	29.985887406254424	37.865605740021266	18.70909229416694	13.439414559557363
7	26.481599296131815	32.50946577804681	23.260558194374955	17.74837673144642
8	24.63396818272389	36.49810936082007	20.91809411125775	17.9498283451983
9	25.784200015098584	16.411306096397617	21.007693416329808	36.79680047217399
10-11	24.389556048561676	26.656646403085695	28.628579370267616	20.32521817808502
12-13	25.281138728162706	24.321627657820386	28.574740788574193	21.822492825442716
14-15	22.341541839474438	25.421686230179237	26.416858812988984	25.81991311735734
16-17	21.938677817651747	28.60095903413033	26.935488815271892	22.524874332946034
18-19	21.972849785405764	27.638659264703897	28.69437575735947	21.694115192530873
20-21	22.91338612399942	26.763051231257297	28.642417551092965	21.68114509365032
22-23	22.6697058195843	26.767883294514522	28.396854843954557	22.165556041946623
24-25	22.44475696416883	26.908665331824373	28.58781584622992	22.058761857776876
26-27	22.313700933523556	26.987105050202924	28.55516844512156	22.14402557115196
28-29	22.306547796297092	27.038595838605552	28.5188386522792	22.136017712818155
30-31	22.712725826453635	26.716244932485594	28.436661737999724	22.134367503061046
32-33	22.426261233608223	26.91820553107298	28.336481503273976	22.31905173204482
34-35	22.619661160583725	27.007342595343204	28.283632356700217	22.089363887372855
36-37	22.676107265017727	26.911025905685715	28.11931165349446	22.293555175802098
38-39	22.336281351105097	27.184311222638975	28.256394546443786	22.22301287981214
40-41	22.562933686907314	26.933668002527174	28.206742053979468	22.296656256586044
42-43	22.43354496517926	27.094352096020373	27.916927912473312	22.555175026327063
44-45	22.433695340960128	27.173664162415516	27.789750501290673	22.602889995333683
46-47	22.620214178011615	27.1228621036353	27.55042051221644	22.70650320613665
48-49	22.704170517831482	27.349481918567143	27.33614917245946	22.610198391141914
50-51	22.656892657845653	27.384425018114488	27.33719271547923	22.621489608560626
52-53	22.889496124559653	27.321665689200987	27.23854051384169	22.55029767239767
54-55	22.90610014581653	27.248457290253697	27.158848475256136	22.686594088673633
56-57	22.868247137677038	27.478294271948382	26.976294168790492	22.677164421584088
58-59	22.703685880027752	27.456930205642777	27.238135376876706	22.60124853745277
60-61	22.813144680267385	27.494473822580982	27.050363792724696	22.642017704426934
62-63	22.789344640663504	27.51359603873484	27.18696318979717	22.510096130804484
64-65	22.87846619220292	27.362782576524395	27.10570338591888	22.653047845353804
66-67	22.919661951310598	27.45613340802494	26.87991812611151	22.744286514552954
68-69	22.841304156916227	27.40909490084254	27.015706603437323	22.73389433880391
70-71	22.859950001680158	27.261125433682153	27.02682180368535	22.852102760952338
72-73	22.83337679403443	27.372387900777216	26.866284698433844	22.927950606754507
74-75	22.726901614402387	27.38575567191544	26.947926158374948	22.939416555307222
76-77	22.859904221806183	27.37244846165025	26.8328746388148	22.934772677728766
78-79	22.658477696455066	27.42846550635525	26.96222685601982	22.95082994116987
80-81	22.79039863449761	27.40850474944785	26.9528589220048	22.84823769404974
82-83	22.947267563455785	27.25658769107137	27.060349534835005	22.73579521063784
84-85	22.822987218095122	27.361402324589264	27.033231870708217	22.78237858660739
86-87	22.67853742898366	27.491387430150432	27.0249866662051	22.805088474660813
88-89	22.871789950979576	27.335773145515844	26.910548196613775	22.881888706890802
90-91	22.89583028670304	27.38802835386098	26.91083814898248	22.805303210453495
92-93	22.827143254936477	27.400686313986867	27.094036157331086	22.67813427374557
94-95	22.607366634677295	27.57231692904401	27.06139049332219	22.758925942956505
96-97	22.633891265504303	27.651664926667134	27.16069820406382	22.553745603764742
98-99	23.793941850694537	29.551079221699972	27.356341740409107	19.298637187196388
100	22.614123960478285	30.703649480183177	21.25581903135363	25.426407527984907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3241.0
1	2650.5
2	2897.0
3	3951.5
4	5071.5
5	7976.0
6	10718.0
7	12709.5
8	14057.0
9	13970.0
10	12748.5
11	11325.0
12	11877.0
13	15293.0
14	23621.5
15	35420.5
16	45577.0
17	51893.5
18	53458.5
19	50579.0
20	45357.0
21	39962.0
22	36573.0
23	37136.5
24	41823.5
25	51254.5
26	63968.0
27	77498.5
28	92797.0
29	110277.5
30	127412.5
31	144283.0
32	163197.5
33	181966.0
34	199516.5
35	217141.5
36	236159.5
37	253188.0
38	266541.0
39	279589.0
40	292180.5
41	302317.0
42	308783.5
43	315934.5
44	323981.0
45	335145.5
46	348038.5
47	362811.5
48	370573.5
49	367142.0
50	363799.5
51	358693.0
52	339514.5
53	314205.5
54	297356.0
55	281745.5
56	263609.5
57	240557.5
58	215972.5
59	190290.5
60	162266.5
61	139415.5
62	116129.0
63	94687.5
64	78853.5
65	62288.5
66	49433.5
67	36761.5
68	24119.0
69	17393.5
70	12580.0
71	8102.0
72	5564.0
73	3935.5
74	2960.5
75	2148.5
76	1401.5
77	788.5
78	594.0
79	481.0
80	317.5
81	208.0
82	172.5
83	119.5
84	72.5
85	44.5
86	31.0
87	24.0
88	15.5
89	10.0
90	6.5
91	4.0
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.862989339718659E-5
10-11	0.0029854003627776166
12-13	2.3448546910705416E-4
14-15	0.003357145618679044
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	191.0
20-21	784.0
22-23	1478.0
24-25	3054.0
26-27	4199.0
28-29	7205.0
30-31	15408.0
32-33	19152.0
34-35	27232.0
36-37	38327.0
38-39	44818.0
40-41	47749.0
42-43	58617.0
44-45	69728.0
46-47	67683.0
48-49	60210.0
50-51	52311.0
52-53	51044.0
54-55	54870.0
56-57	58077.0
58-59	58980.0
60-61	58444.0
62-63	57204.0
64-65	61494.0
66-67	64721.0
68-69	65323.0
70-71	64626.0
72-73	62466.0
74-75	65855.0
76-77	61108.0
78-79	70704.0
80-81	72257.0
82-83	70232.0
84-85	72760.0
86-87	78072.0
88-89	85799.0
90-91	90643.0
92-93	99771.0
94-95	125753.0
96-97	314156.0
98-99	552803.0
100-101	5807238.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.76735503339036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.81575893082677	76.13444324882794
2	7.082608691493755	12.00748011028855
3	1.7112854112625493	4.351834140598617
4	0.6256932902447998	2.121534611047644
5	0.2865774868538217	1.2146207786357328
6	0.14433808188928693	0.7341094459408652
7	0.09445695826372785	0.5604806561560888
8	0.059347329683692676	0.40245729324649937
9	0.03851169546819606	0.2938081106421347
>10	0.13700819337799797	1.8541261178952513
>50	0.0035347035239964427	0.20326770717993778
>100	8.32788436054202E-4	0.09847482308377822
>500	4.643867536155521E-5	0.023362956456960078
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.1438315566197764E-5	0.0	0.0	0.0
4	0.0	3.4314946698593295E-5	0.0	0.0	0.0
5	0.0	3.4314946698593295E-5	0.0	0.0	0.0
6	2.2876631132395527E-5	3.4314946698593295E-5	0.0	0.0	1.1438315566197764E-5
7	2.2876631132395527E-5	3.4314946698593295E-5	0.0	1.1438315566197764E-5	1.1438315566197764E-5
8	2.2876631132395527E-5	3.4314946698593295E-5	0.0	1.1438315566197764E-5	1.1438315566197764E-5
9	2.2876631132395527E-5	1.258214712281754E-4	0.0	3.4314946698593295E-5	1.1438315566197764E-5
10-11	2.2876631132395527E-5	1.258214712281754E-4	0.0	3.4314946698593295E-5	2.2876631132395527E-5
12-13	2.2876631132395527E-5	1.258214712281754E-4	0.0	3.4314946698593295E-5	4.0034104481692175E-5
14-15	2.2876631132395527E-5	1.94451364625362E-4	0.0	3.4314946698593295E-5	4.5753262264791055E-5
16-17	5.719157783098882E-5	1.94451364625362E-4	0.0	3.4314946698593295E-5	6.29107356140877E-5
18-19	5.719157783098882E-5	2.0588968019155977E-4	0.0	3.4314946698593295E-5	8.006820896338435E-5
20-21	5.719157783098882E-5	2.287663113239553E-4	0.0	3.4314946698593295E-5	1.1438315566197764E-4
22-23	5.719157783098882E-5	2.287663113239553E-4	0.0	5.147242004788994E-5	1.1438315566197764E-4
24-25	5.719157783098882E-5	2.802387313718452E-4	0.0	6.862989339718659E-5	1.315406290112743E-4
26-27	5.719157783098882E-5	2.9739620472114185E-4	0.0	1.5441726014366983E-4	1.3725978679437318E-4
28-29	5.719157783098882E-5	3.031153625042408E-4	0.0	5.147242004788994E-4	1.3725978679437318E-4
30-31	6.29107356140877E-5	3.7746441368452624E-4	0.0	0.0018758837528564335	1.4297894457747205E-4
32-33	6.862989339718659E-5	3.7746441368452624E-4	0.0	0.005124365373656598	1.601364179267687E-4
34-35	6.862989339718659E-5	4.6325178043100945E-4	0.0	0.009533836024425837	1.601364179267687E-4
36-37	6.862989339718659E-5	5.261625160450971E-4	0.0	0.015567547485595159	1.601364179267687E-4
38-39	6.862989339718659E-5	5.661966205267894E-4	0.0	0.021950127571533508	1.601364179267687E-4
40-41	6.862989339718659E-5	6.748606184056681E-4	0.0	0.029865441943342362	1.601364179267687E-4
42-43	6.862989339718659E-5	6.920180917549647E-4	0.0	0.0352872035217201	1.601364179267687E-4
44-45	6.862989339718659E-5	7.377713540197558E-4	0.0	0.043271147786926145	1.601364179267687E-4
46-47	6.862989339718659E-5	7.663671429352502E-4	0.0	0.04937920829927575	1.658555757098676E-4
48-49	6.862989339718659E-5	7.77805458501448E-4	0.0	0.05573319259629861	1.8301304905916422E-4
50-51	8.578736674648324E-5	8.750311408141289E-4	0.0	0.061435192906048194	1.8301304905916422E-4
52-53	9.150652452958211E-5	8.807502985972278E-4	0.0	0.06698849511343721	2.0588968019155977E-4
54-55	9.150652452958211E-5	9.093460875127222E-4	0.0	0.07178114933567406	2.287663113239553E-4
56-57	9.150652452958211E-5	9.265035608620189E-4	0.0	0.07799215468811946	2.4592378467325194E-4
58-59	9.150652452958211E-5	9.379418764282166E-4	0.0	0.08409449604268596	2.6880041580564743E-4
60-61	1.0294484009577988E-4	9.608185075606122E-4	0.0	0.08866410311138197	2.7451957358874636E-4
62-63	1.1438315566197764E-4	9.951334542592056E-4	0.0	0.09267895187511739	2.7451957358874636E-4
64-65	1.1438315566197764E-4	0.0011323932410535788	0.0	0.0972142439971148	2.7451957358874636E-4
66-67	1.315406290112743E-4	0.0011838656611014686	0.0	0.10160655717453473	2.859578891549441E-4
68-69	1.4297894457747205E-4	0.0012010231344507653	0.0	0.10627910908332652	3.2599199363663627E-4
70-71	1.4869810236057093E-4	0.001218180607800062	0.0	0.11053416247395209	3.488686247690318E-4
72-73	1.7157473349296647E-4	0.0012238997655831607	0.0	0.11373689083248748	4.0034104481692173E-4
74-75	1.7157473349296647E-4	0.0012639338700648531	0.0	0.11627619688818337	4.0034104481692173E-4
76-77	1.7729389127606535E-4	0.0013096871323296441	0.0	0.11858101747477222	4.0034104481692173E-4
78-79	1.8301304905916422E-4	0.001315406290112743	0.0	0.12009087512951033	4.0034104481692173E-4
80-81	1.8301304905916422E-4	0.0013382829212451384	0.0	0.12057700354107373	4.1749851816621835E-4
82-83	1.8301304905916422E-4	0.001349721236811336	0.0	0.12097734458589066	4.3465599151551503E-4
84-85	1.8301304905916422E-4	0.0013668787101606327	0.0	0.12118323426608221	4.3465599151551503E-4
86-87	1.8301304905916422E-4	0.001406912814642325	0.0	0.12123470668613011	4.3465599151551503E-4
88	1.8301304905916422E-4	0.0014641043924733138	0.0	0.12123470668613011	4.4609430708171283E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11010	0.0	54.906334	1
GTATCAA	20335	0.0	38.472767	1
TCAACGC	25410	0.0	30.432972	4
ATCAACG	25725	0.0	30.01011	3
CAACGCA	26195	0.0	29.474358	5
TATCAAC	26700	0.0	29.010994	2
AACGCAG	26965	0.0	28.696577	6
ACGCAGA	31120	0.0	24.892815	7
CGCAGAG	34155	0.0	22.680979	8
GTACATG	27800	0.0	21.946625	1
TACATGG	27810	0.0	21.412329	2
GTGGTAT	4780	0.0	20.987984	1
GCAGAGT	36850	0.0	20.778131	9
ACATGGG	28205	0.0	20.685017	3
GAGTACT	21570	0.0	19.533642	12-13
AGAGTAC	33375	0.0	19.019035	10-11
CAGAGTA	35770	0.0	18.97961	10-11
TGGTATC	4770	0.0	18.955868	2
CATGGGG	20880	0.0	18.930187	4
TATATAC	10750	0.0	18.664661	3
>>END_MODULE