FastQCFastQC Report
Fri 27 May 2016
SRR2139242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747516
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114550.053.857291
GTATCAA212300.037.1238861
TCAACGC265650.029.4274584
ATCAACG269050.029.0397133
CAACGCA273300.028.6367915
TATCAAC274150.028.5771942
AACGCAG280000.027.9668036
ACGCAGA320150.024.446437
CGCAGAG352700.022.214538
GTACATG289400.021.9373511
TACATGG289100.021.4284652
GTGGTAT53400.020.947481
ACATGGG292100.020.7115463
CTTATAC50550.020.5237561
GCAGAGT379250.020.5140619
GAGTACT217000.019.87287512-13
TGGTATC52800.019.8108842
TATATAC105400.019.0384453
CAGAGTA367600.018.80024710-11
CATGGGG221750.018.7914544
Produced by FastQC (version 0.11.5)