##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8747516
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28466904204576	33.0	33.0	33.0	33.0	33.0
2	32.32988759323218	33.0	33.0	33.0	33.0	33.0
3	32.35536625483166	33.0	33.0	33.0	33.0	33.0
4	36.217462420188774	37.0	37.0	37.0	33.0	37.0
5	36.26535384445138	37.0	37.0	37.0	33.0	37.0
6	36.252416114471814	37.0	37.0	37.0	33.0	37.0
7	36.215471112027686	37.0	37.0	37.0	33.0	37.0
8	36.2009177233857	37.0	37.0	37.0	33.0	37.0
9	36.26776035619712	37.0	37.0	37.0	33.0	37.0
10-11	36.18649637222727	37.0	37.0	37.0	33.0	37.0
12-13	36.14292943276698	37.0	37.0	37.0	33.0	37.0
14-15	37.873521980411354	40.0	37.0	40.0	33.0	40.0
16-17	37.80419327040957	40.0	37.0	40.0	33.0	40.0
18-19	37.76992148399614	40.0	37.0	40.0	33.0	40.0
20-21	37.77728736445103	40.0	37.0	40.0	33.0	40.0
22-23	37.78186691036852	40.0	37.0	40.0	33.0	40.0
24-25	37.77693329989653	40.0	37.0	40.0	33.0	40.0
26-27	37.636527113265885	40.0	37.0	40.0	33.0	40.0
28-29	37.559532227409605	40.0	37.0	40.0	33.0	40.0
30-31	37.438565078607425	40.0	37.0	40.0	33.0	40.0
32-33	37.37990231254014	40.0	37.0	40.0	33.0	40.0
34-35	37.345287467116485	40.0	37.0	40.0	33.0	40.0
36-37	37.284252151859164	40.0	37.0	40.0	33.0	40.0
38-39	37.216880591059564	40.0	37.0	40.0	33.0	40.0
40-41	37.184042575707025	40.0	37.0	40.0	33.0	40.0
42-43	37.0939091666106	40.0	37.0	40.0	33.0	40.0
44-45	37.02517604438998	37.0	37.0	40.0	33.0	40.0
46-47	36.96849216490634	37.0	37.0	40.0	33.0	40.0
48-49	36.94114588186814	37.0	37.0	40.0	33.0	40.0
50-51	36.84274648440507	37.0	37.0	40.0	33.0	40.0
52-53	36.71946025968606	37.0	37.0	40.0	33.0	40.0
54-55	36.519675706459026	37.0	37.0	40.0	33.0	40.0
56-57	36.410778688454755	37.0	37.0	40.0	33.0	40.0
58-59	36.25492253406343	37.0	37.0	40.0	33.0	40.0
60-61	36.10428858839592	37.0	37.0	40.0	33.0	40.0
62-63	35.9260956256915	37.0	33.0	40.0	33.0	40.0
64-65	35.74161740718729	37.0	33.0	40.0	27.0	40.0
66-67	35.53138619032124	37.0	33.0	38.5	27.0	40.0
68-69	35.31775489776214	37.0	33.0	37.0	27.0	40.0
70-71	35.088814804786836	37.0	33.0	37.0	27.0	40.0
72-73	34.88115583063018	37.0	33.0	37.0	27.0	40.0
74-75	34.69672441209137	37.0	33.0	37.0	27.0	40.0
76-77	32.70487562066671	33.0	33.0	37.0	27.0	37.0
78-79	34.169429962267486	37.0	33.0	37.0	27.0	37.0
80-81	34.12239844661788	37.0	33.0	37.0	27.0	37.0
82-83	33.808548073750416	37.0	33.0	37.0	27.0	37.0
84-85	33.90994122469718	37.0	33.0	37.0	27.0	37.0
86-87	33.86231726310825	37.0	33.0	37.0	27.0	37.0
88-89	33.751788060346115	37.0	33.0	37.0	27.0	37.0
90-91	33.42469471318017	33.0	33.0	37.0	27.0	37.0
92-93	33.566888075342625	33.0	33.0	37.0	27.0	37.0
94-95	33.48129838116235	33.0	33.0	37.0	27.0	37.0
96-97	33.52462339355427	33.0	33.0	37.0	27.0	37.0
98-99	33.68249041356341	33.0	33.0	37.0	27.0	37.0
100	33.72557682762192	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	1.0
18	4.0
19	22.0
20	133.0
21	671.0
22	2525.0
23	7116.0
24	16094.0
25	30684.0
26	52549.0
27	81813.0
28	117100.0
29	161096.0
30	212776.0
31	274818.0
32	353702.0
33	457005.0
34	600469.0
35	824781.0
36	1309018.0
37	2344013.0
38	1859864.0
39	41260.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.7460453916289	20.0992830421802	14.142037579582592	24.01263398660831
2	14.924362527602122	21.280544099604963	40.90943074582544	22.885662626967473
3	18.049672615631685	26.24445614046319	30.415960370921297	25.28991087298383
4	12.52853953053644	18.332953034895848	38.32084445458573	30.817662979981975
5	13.707022656488995	37.47522153717696	35.32408514600031	13.493670660333745
6	30.12984486110114	37.695284009769175	18.63454722460639	13.540323904523296
7	26.520180128850292	32.52830860783793	23.1226670519951	17.828844211316675
8	24.73363866953773	36.33448627015944	20.905077510003984	18.026797550298852
9	25.781316358146594	16.399586945455237	20.996219153946956	36.82287754245121
10-11	24.44577425951568	26.584429754363885	28.554209278163828	20.415586707956606
12-13	25.322669850514767	24.298889989649595	28.49926824689974	21.8791719129359
14-15	22.38469479446041	25.403522490574147	26.32171135515055	25.890071359814893
16-17	22.01214607666908	28.569853430390985	26.84461508844339	22.573385404496545
18-19	22.092037328082622	27.548797853013358	28.57230555508558	21.786859263818435
20-21	22.99503288280059	26.705342989228694	28.548525538559748	21.751098589410965
22-23	22.75259666364323	26.70403139583955	28.298622921894456	22.244749018622755
24-25	22.484470898932354	26.90018053716497	28.530270110406036	22.08507845349664
26-27	22.3741618436134	26.917167964446126	28.50196800709906	22.206702184841415
28-29	22.371455724202782	26.911439476203803	28.491375069195378	22.22572973039804
30-31	22.753048222322754	26.633806194458774	28.421445297312875	22.19170028590559
32-33	22.5166049961474	26.850659552381746	28.265578434755888	22.367157016714966
34-35	22.64418804261474	26.954112368141196	28.269501755757602	22.132197833486458
36-37	22.80023678187371	26.834776482809513	28.013354331319952	22.351632403996827
38-39	22.397402326164702	27.109781369959613	28.17043975203938	22.32237655183631
40-41	22.640747673588553	26.85624081794893	28.15785218947959	22.345159318982923
42-43	22.493679012029567	27.04646408457888	27.873582237326417	22.58627466606514
44-45	22.4603675940494	27.10531196564353	27.732375046914775	22.701945393392293
46-47	22.73043119534163	27.036629410094825	27.464156109244946	22.768783285318595
48-49	22.764876157914454	27.312269475908764	27.261338728540984	22.661515637635798
50-51	22.704564079265264	27.320329905962122	27.30368538952354	22.671420625249077
52-53	22.95321417756045	27.258913930959235	27.209604681892735	22.57826720958758
54-55	22.947064074315644	27.185742171893157	27.11182046640247	22.755373287388732
56-57	22.964018304247038	27.454448625166545	26.89041072959697	22.69112234098945
58-59	22.710098255728155	27.453869635354188	27.186286987780427	22.64974512113723
60-61	22.88930346275211	27.486003404654756	26.972928853405893	22.651764279187237
62-63	22.85969273921081	27.508594252939528	27.092191901378797	22.539521106470858
64-65	22.951821152512704	27.363783237682238	27.018181720289096	22.666213889515966
66-67	22.97250679239614	27.450377044787178	26.79442897354042	22.782687189276263
68-69	22.89215688195629	27.34852709739223	26.943078317265744	22.816237703385735
70-71	22.92474350221438	27.219023730859615	26.94513458813151	22.911098178794497
72-73	22.887403603079377	27.306665210823223	26.822801437822946	22.983129748274457
74-75	22.770269312137064	27.332699674583992	26.876091712191148	23.020939301087793
76-77	22.92822697919325	27.34071750151551	26.753126929593435	22.9779285896978
78-79	22.710532618665898	27.401413139158333	26.884395129360723	23.00365911281505
80-81	22.857929031591066	27.311650836071085	26.882209725526323	22.948210406811523
82-83	23.00664725929566	27.205451384633733	26.99697174103765	22.790929615032958
84-85	22.876896649855574	27.298255015185102	26.992732305757063	22.832116029202265
86-87	22.725230048620052	27.442900322728892	26.940043009494946	22.89182661915611
88-89	22.90413072980013	27.275737801427784	26.831868274257314	22.988263194514765
90-91	22.926382485290453	27.314394124544254	26.85965847824	22.89956491192529
92-93	22.856461668477387	27.366013887902024	27.01760221611102	22.759922227509563
94-95	22.646653403962716	27.490562311239575	27.043042943241186	22.819741341556522
96-97	22.6823625279423	27.54602185228048	27.108924904701777	22.662690715075453
98-99	23.885245619740285	29.506735984265887	27.210862283409682	19.397156112584145
100	22.70464515699909	30.58279699662112	21.14745022423614	25.56510762214365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3428.0
1	2850.5
2	3140.5
3	4148.0
4	5148.5
5	7877.0
6	10460.0
7	12582.0
8	14315.5
9	14488.0
10	13169.5
11	11500.5
12	11831.0
13	15016.5
14	23306.5
15	35309.5
16	45432.0
17	51429.0
18	52771.5
19	50051.0
20	45500.5
21	40808.5
22	37659.5
23	38670.0
24	44068.5
25	53692.0
26	66738.5
27	81116.5
28	96581.0
29	113709.0
30	130106.0
31	145877.5
32	163325.5
33	181245.0
34	198585.0
35	215072.0
36	233450.5
37	250648.5
38	263392.0
39	276660.0
40	289973.0
41	301094.5
42	308474.0
43	314443.0
44	322951.5
45	336048.0
46	350109.0
47	366035.5
48	373763.0
49	369981.5
50	367455.5
51	363522.5
52	346024.5
53	319555.5
54	301175.5
55	286358.5
56	269005.5
57	247430.0
58	223533.0
59	197405.5
60	168401.0
61	144868.5
62	120874.0
63	98318.5
64	82066.0
65	65653.5
66	52800.0
67	39428.0
68	25976.5
69	18793.0
70	13631.0
71	8873.0
72	6160.0
73	4446.5
74	3355.0
75	2500.0
76	1639.5
77	925.0
78	688.0
79	575.5
80	402.5
81	266.5
82	202.0
83	118.0
84	54.5
85	33.5
86	27.0
87	23.5
88	19.5
89	14.5
90	10.0
91	7.5
92	5.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.42954502741121E-5
10-11	0.0030637268911540144
12-13	3.42954502741121E-4
14-15	0.0033838177603790604
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	235.0
20-21	801.0
22-23	1662.0
24-25	3469.0
26-27	4502.0
28-29	7468.0
30-31	15776.0
32-33	20063.0
34-35	28289.0
36-37	39886.0
38-39	47034.0
40-41	50088.0
42-43	61519.0
44-45	73673.0
46-47	71479.0
48-49	63946.0
50-51	57422.0
52-53	56837.0
54-55	62293.0
56-57	65101.0
58-59	66980.0
60-61	65956.0
62-63	65203.0
64-65	70625.0
66-67	74131.0
68-69	73358.0
70-71	72536.0
72-73	69526.0
74-75	72749.0
76-77	69783.0
78-79	79252.0
80-81	80272.0
82-83	78110.0
84-85	79953.0
86-87	86568.0
88-89	94433.0
90-91	98435.0
92-93	107296.0
94-95	131267.0
96-97	316444.0
98-99	540229.0
100-101	5622867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.71881145066143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99854725431355	76.24569955671633
2	6.955492032771329	11.785220361418643
3	1.6511835826260712	4.1965893182077725
4	0.6059581378180933	2.0534421289921982
5	0.2727434923434641	1.155325225112043
6	0.154160593006752	0.7836181327236708
7	0.0947839870962293	0.5620990711943163
8	0.05977179451261702	0.4051036311505665
9	0.044369392287300115	0.3383029961431389
>10	0.1583615648125807	2.1341657169667045
>50	0.003704657110438008	0.21558820685884617
>100	8.9961578921846E-4	0.11320535792595089
>500	2.3895512368995248E-5	0.011640296589708677
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1431816758037367E-5	0.0
4	0.0	6.85909005482242E-5	0.0	1.1431816758037367E-5	0.0
5	0.0	1.028863508223363E-4	0.0	2.2863633516074735E-5	0.0
6	0.0	1.028863508223363E-4	0.0	3.42954502741121E-5	0.0
7	0.0	1.028863508223363E-4	0.0	3.42954502741121E-5	0.0
8	0.0	1.028863508223363E-4	0.0	4.572726703214947E-5	0.0
9	0.0	1.9434088488663525E-4	0.0	5.715908379018684E-5	0.0
10-11	0.0	1.9434088488663525E-4	0.0	7.430680892724289E-5	0.0
12-13	0.0	2.0005679326565393E-4	0.0	1.028863508223363E-4	5.715908379018684E-6
14-15	0.0	3.200908692250463E-4	0.0	1.028863508223363E-4	2.2863633516074735E-5
16-17	0.0	3.3152268598308365E-4	0.0	1.028863508223363E-4	4.572726703214947E-5
18-19	0.0	3.601022278781771E-4	0.0	1.028863508223363E-4	5.715908379018684E-5
20-21	0.0	4.0011358653130785E-4	0.0	1.371818010964484E-4	5.715908379018684E-5
22-23	0.0	4.3440903680541995E-4	0.0	1.5432952623350445E-4	5.715908379018684E-5
24-25	0.0	4.629885787005134E-4	0.0	2.0005679326565393E-4	5.715908379018684E-5
26-27	0.0	4.915681205956068E-4	0.0	2.457840602978034E-4	5.715908379018684E-5
28-29	0.0	4.915681205956068E-4	0.0	3.772499530152331E-4	8.002271730626158E-5
30-31	0.0	6.173181049340178E-4	0.0	9.659885160541576E-4	8.002271730626158E-5
32-33	1.1431816758037367E-5	6.230340133130365E-4	0.0	0.0028751019146463982	8.002271730626158E-5
34-35	1.1431816758037367E-5	6.973408222402794E-4	0.0	0.005681612928744572	9.145453406429894E-5
36-37	1.1431816758037367E-5	7.659317227885036E-4	0.0	0.008470976217705688	9.145453406429894E-5
38-39	1.714772513705605E-5	7.830794479255596E-4	0.0	0.0124206689076076	9.145453406429894E-5
40-41	2.2863633516074735E-5	8.573862568528025E-4	0.0	0.016970531977306472	9.145453406429894E-5
42-43	2.2863633516074735E-5	8.85965798747896E-4	0.0	0.021217451902917354	9.145453406429894E-5
44-45	2.857954189509342E-5	0.0010002839663282696	0.0	0.028373769193448747	9.145453406429894E-5
46-47	3.42954502741121E-5	0.0010974544087715872	0.0	0.034061098030572334	9.717044244331763E-5
48-49	3.42954502741121E-5	0.0011260339506666806	0.0	0.03990847230230845	1.028863508223363E-4
50-51	3.42954502741121E-5	0.0012174884847309798	0.0	0.04591589200865709	1.028863508223363E-4
52-53	3.42954502741121E-5	0.0012232043931099984	0.0	0.05200333443231198	1.028863508223363E-4
54-55	3.42954502741121E-5	0.001263215751763129	0.0	0.057301981499662306	1.028863508223363E-4
56-57	3.42954502741121E-5	0.0012689316601421478	0.0	0.06490985555213617	1.028863508223363E-4
58-59	3.42954502741121E-5	0.0012803634769001852	0.0	0.07297500227493153	1.028863508223363E-4
60-61	3.42954502741121E-5	0.0013203748355533158	0.0	0.07863946747853905	1.028863508223363E-4
62-63	3.42954502741121E-5	0.0013432384690693906	0.0	0.08414388724753405	1.028863508223363E-4
64-65	3.42954502741121E-5	0.001514715720439951	0.0	0.08969975019194021	1.028863508223363E-4
66-67	3.42954502741121E-5	0.0015375793539560258	0.0	0.09519273814417717	1.028863508223363E-4
68-69	3.42954502741121E-5	0.0015947384377462126	0.0	0.10084005562264761	1.028863508223363E-4
70-71	3.42954502741121E-5	0.0016176020712622874	0.0	0.10593292998835327	1.028863508223363E-4
72-73	3.42954502741121E-5	0.0016347497963993436	0.0	0.10969399770174756	1.028863508223363E-4
74-75	3.42954502741121E-5	0.0017033406969475676	0.0	0.11284346321858685	1.028863508223363E-4
76-77	3.42954502741121E-5	0.0017947952310118668	0.0	0.11577000830864442	1.028863508223363E-4
78-79	3.42954502741121E-5	0.0018405224980440162	0.0	0.1179363375842925	1.028863508223363E-4
80-81	3.42954502741121E-5	0.001846238406423035	0.0	0.11869083749032297	1.028863508223363E-4
82-83	3.42954502741121E-5	0.0018576702231810722	0.0	0.1192795760533619	1.1431816758037368E-4
84-85	3.42954502741121E-5	0.0018633861315600908	0.0	0.11953107602203872	1.1431816758037368E-4
86-87	3.42954502741121E-5	0.0018862497650761656	0.0	0.11963967828124006	1.1431816758037368E-4
88	3.42954502741121E-5	0.0019662724823824272	0.0	0.11964539418961909	1.1431816758037368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11455	0.0	53.85729	1
GTATCAA	21230	0.0	37.123886	1
TCAACGC	26565	0.0	29.427458	4
ATCAACG	26905	0.0	29.039713	3
CAACGCA	27330	0.0	28.636791	5
TATCAAC	27415	0.0	28.577194	2
AACGCAG	28000	0.0	27.966803	6
ACGCAGA	32015	0.0	24.44643	7
CGCAGAG	35270	0.0	22.21453	8
GTACATG	28940	0.0	21.937351	1
TACATGG	28910	0.0	21.428465	2
GTGGTAT	5340	0.0	20.94748	1
ACATGGG	29210	0.0	20.711546	3
CTTATAC	5055	0.0	20.523756	1
GCAGAGT	37925	0.0	20.514061	9
GAGTACT	21700	0.0	19.872875	12-13
TGGTATC	5280	0.0	19.810884	2
TATATAC	10540	0.0	19.038445	3
CAGAGTA	36760	0.0	18.800247	10-11
CATGGGG	22175	0.0	18.791454	4
>>END_MODULE