FastQCFastQC Report
Fri 27 May 2016
SRR2139241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23091546
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA290350.049.855951
GTATCAA518650.036.3598941
TCAACGC616850.029.9356774
ATCAACG623300.029.6258963
CAACGCA631300.029.2915335
AACGCAG645750.028.6875236
TATCAAC657150.028.3094752
ACGCAGA742000.024.9261677
CGCAGAG750500.024.6209228
GTACATG647300.021.2370071
TACATGG643650.020.7006992
GCAGAGT890000.020.6990689
ACATGGG650300.019.7994923
GAGTACT545050.019.66473612-13
CAGAGTA868650.018.67262610-11
AGAGTAC802100.018.60865410-11
GTGGTAT150450.018.0046331
CATGGGG442300.017.9637134
GTACTTT612700.017.40227914-15
TGGTATC139900.017.3583152
Produced by FastQC (version 0.11.5)