FastQCFastQC Report
Fri 27 May 2016
SRR2139240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523722
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185100.047.57721
GTATCAA327650.035.737151
TCAACGC388200.029.5685674
ATCAACG391200.029.319963
CAACGCA396650.028.9060945
AACGCAG402400.028.586156
TATCAAC411950.028.0193042
ACGCAGA455050.025.1949487
CGCAGAG458950.024.9718348
GCAGAGT535600.021.2147839
GTACATG361500.020.3403421
TACATGG353700.020.1966552
GAGTACT335550.019.98225812-13
ACATGGG357100.019.5855143
CAGAGTA527450.018.87595410-11
AGAGTAC486350.018.72196410-11
CTTATAC120550.017.83771
GTACTTT386100.017.38836914-15
CATGGGG245550.017.2882424
AGTACTT363300.017.07925212-13
Produced by FastQC (version 0.11.5)