##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12523722
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.513726989468466	33.0	33.0	33.0	33.0	33.0
2	32.5155143973972	33.0	33.0	33.0	33.0	33.0
3	32.51223853419934	33.0	33.0	33.0	33.0	33.0
4	36.40471187399401	37.0	37.0	37.0	37.0	37.0
5	36.43097267729194	37.0	37.0	37.0	37.0	37.0
6	36.42148883534783	37.0	37.0	37.0	37.0	37.0
7	36.39638471693958	37.0	37.0	37.0	37.0	37.0
8	36.38397993823242	37.0	37.0	37.0	37.0	37.0
9	36.42898684592328	37.0	37.0	37.0	37.0	37.0
10-11	36.368750080846574	37.0	37.0	37.0	37.0	37.0
12-13	36.349989603729625	37.0	37.0	37.0	37.0	37.0
14-15	38.254669498412696	40.0	37.0	40.0	37.0	40.0
16-17	38.20779812902266	40.0	37.0	40.0	35.0	40.0
18-19	38.22133615709451	40.0	37.0	40.0	37.0	40.0
20-21	38.223990225663485	40.0	37.0	40.0	33.0	40.0
22-23	38.229488660946146	40.0	37.0	40.0	37.0	40.0
24-25	38.207519657827206	40.0	37.0	40.0	35.0	40.0
26-27	38.076924078300024	40.0	37.0	40.0	33.0	40.0
28-29	38.01081353128777	40.0	37.0	40.0	33.0	40.0
30-31	37.94907790535554	40.0	37.0	40.0	33.0	40.0
32-33	37.884323905711	40.0	37.0	40.0	33.0	40.0
34-35	37.833993817337735	40.0	37.0	40.0	33.0	40.0
36-37	37.78028524814527	40.0	37.0	40.0	33.0	40.0
38-39	37.72703989097858	40.0	37.0	40.0	33.0	40.0
40-41	37.70948659225795	40.0	37.0	40.0	33.0	40.0
42-43	37.65083795836398	40.0	37.0	40.0	33.0	40.0
44-45	37.570216547092954	40.0	37.0	40.0	33.0	40.0
46-47	37.53335851646533	40.0	37.0	40.0	33.0	40.0
48-49	37.53966194373619	40.0	37.0	40.0	33.0	40.0
50-51	37.45054030951951	40.0	37.0	40.0	33.0	40.0
52-53	37.337600419236324	40.0	37.0	40.0	33.0	40.0
54-55	37.21420011243867	40.0	37.0	40.0	33.0	40.0
56-57	37.10645922399085	38.5	37.0	40.0	33.0	40.0
58-59	36.98775028202097	37.0	37.0	40.0	33.0	40.0
60-61	36.83591004460476	37.0	37.0	40.0	33.0	40.0
62-63	36.6623385028021	37.0	37.0	40.0	33.0	40.0
64-65	36.51725683673328	37.0	37.0	40.0	33.0	40.0
66-67	36.30338010316161	37.0	37.0	40.0	33.0	40.0
68-69	36.11674012471623	37.0	37.0	40.0	33.0	40.0
70-71	35.872070270896245	37.0	35.0	38.5	33.0	40.0
72-73	35.65972349470904	37.0	33.0	37.0	33.0	40.0
74-75	35.45681899965152	37.0	33.0	37.0	33.0	40.0
76-77	33.672431736134044	35.0	33.0	37.0	27.0	37.0
78-79	34.911537144224084	37.0	33.0	37.0	30.0	37.0
80-81	34.99019936355898	37.0	33.0	37.0	33.0	37.0
82-83	34.83694812522158	37.0	33.0	37.0	33.0	37.0
84-85	34.664464288528265	37.0	33.0	37.0	33.0	37.0
86-87	34.52102147520061	37.0	33.0	37.0	30.0	37.0
88-89	34.388759510580215	37.0	33.0	37.0	27.0	37.0
90-91	34.32333635374055	37.0	33.0	37.0	27.0	37.0
92-93	34.27799394053157	37.0	33.0	37.0	27.0	37.0
94-95	34.286620379200926	37.0	33.0	37.0	27.0	37.0
96-97	34.24915764616188	37.0	33.0	37.0	27.0	37.0
98-99	34.276927961376956	37.0	33.0	37.0	27.0	37.0
100	34.27964203771807	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	4.0
19	11.0
20	108.0
21	625.0
22	2340.0
23	6470.0
24	14951.0
25	29498.0
26	50470.0
27	79715.0
28	116610.0
29	163060.0
30	219885.0
31	289439.0
32	379791.0
33	504657.0
34	680653.0
35	959740.0
36	1590474.0
37	3436068.0
38	3896289.0
39	102863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.784999858668215	20.889492756226943	14.666334816438756	23.659172568666087
2	14.187627288437096	21.419271363577057	42.39177458586194	22.001326762123913
3	17.51682127725288	27.11555718020569	31.09011043202652	24.27751111051491
4	11.978110031943832	18.810360804388853	39.812113902253714	29.3994152614136
5	12.795956175935094	38.181221452071426	36.16985019324751	12.852972178745967
6	28.718190065077305	38.91305148046655	19.308750170975543	13.060008283480604
7	25.29311174425622	33.29334522117306	24.02461504654926	17.38892798802145
8	23.658821235412283	37.301394904805456	21.706390480401915	17.333393379380347
9	25.165383597000613	17.026988699426333	21.81801065060144	35.98961705297162
10-11	23.487401451602736	27.213313290649406	29.597135585275343	19.702149672472515
12-13	24.50406184342514	24.871237716903636	29.554693402935467	21.070007036735753
14-15	21.67655580744029	26.211239584702	27.30384601119224	24.808358596665467
16-17	21.044937758918635	29.315294606507553	27.97998869665104	21.65977893792277
18-19	21.03010191379208	28.373577759071942	29.820416007317952	20.77590431981802
20-21	21.853048302622657	27.485918154755545	29.739432612260398	20.921600930361397
22-23	21.515788133714814	27.595050858342972	29.559019791623285	21.33014121631893
24-25	21.316577887164332	27.735089287051508	29.676094434099316	21.272238391684848
26-27	21.29919498480174	27.883680269924238	29.553651126307727	21.26347361896629
28-29	21.154495661316236	27.912747191327213	29.558594046841307	21.374163100515243
30-31	21.37175919598867	27.77243503484944	29.564216397296246	21.291589371865648
32-33	21.23312653468958	27.891724477417597	29.503143522236584	21.372005465656237
34-35	21.342099510677766	28.070717429367058	29.474577352631115	21.112605707324057
36-37	21.424704292661527	27.970761708399642	29.200838377539785	21.403695621399045
38-39	21.318982647616032	28.013566342189545	29.345714833407634	21.321736176786793
40-41	21.537074019985475	27.91841705066023	29.239127908091127	21.305381021263166
42-43	21.505612702206665	28.001346950032342	29.03832200067635	21.454718347084643
44-45	21.562848357448615	28.19974743723072	28.75901779454103	21.478386410779628
46-47	21.670445303244453	28.12065096873726	28.4085158909299	21.80038783708839
48-49	21.63127952683587	28.219359601348604	28.40773293952085	21.741627932294676
50-51	21.57781674653887	28.36654203061016	28.30485224960358	21.750788973247396
52-53	21.732131887761515	28.308724427133342	28.271135678351634	21.688008006753513
54-55	21.74955705099015	28.243132807690767	28.192107935332455	21.81520220598663
56-57	21.764325048251067	28.369378389975296	28.10338318002823	21.76291338174541
58-59	21.65429762158011	28.440444093222673	28.232014066484446	21.673244218712778
60-61	21.854790851006385	28.492108600236193	28.056403259142492	21.596697289614934
62-63	21.72146170204562	28.5742233422773	28.009072375113853	21.69524258056323
64-65	21.694660066590583	28.50739353604791	28.01724780264106	21.780698594720448
66-67	21.760833982491807	28.45027032020358	27.90288574185996	21.886009955444656
68-69	21.751483917730674	28.408341547808703	27.95271745241555	21.887457082045074
70-71	21.760301516349003	28.213049241659817	28.0083280204365	22.01832122155468
72-73	21.80928794674641	28.203808660609965	27.981398544901904	22.005504847741722
74-75	21.745641629514328	28.355725958950227	27.913679671109954	21.98495274042549
76-77	21.83405867726859	28.292841034164297	27.827304000564606	22.045796288002506
78-79	21.789207389217818	28.292839160159268	27.835834889956672	22.08211856066624
80-81	21.782810835405577	28.265376474635882	27.888358652133068	22.06345403782547
82-83	21.83150875669545	28.285004563371334	27.933575487853673	21.94991119207954
84-85	21.958358437707677	28.26532110668407	27.859089596299004	21.917230859309246
86-87	21.784059992348435	28.265518835348445	27.942172159659513	22.00824901264361
88-89	21.84148159179387	28.268028269333094	27.90854553755251	21.981944601320528
90-91	21.853247392116604	28.256170382480384	27.926919731373584	21.963662494029425
92-93	21.827683785961465	28.226495102341204	28.004366571788776	21.94145453990856
94-95	21.695166193196126	28.357086611529176	28.025027619114777	21.922719576159917
96-97	21.74922485358167	28.42724208577141	28.103331000296443	21.720202060350484
98-99	22.86773477239053	30.299507208315724	28.33589094122043	18.49686707807331
100	21.82174299255795	31.363902199808454	22.164213545537848	24.650141262095755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3431.0
1	3047.0
2	4320.0
3	6301.5
4	8632.5
5	14184.5
6	18629.0
7	21018.5
8	21964.5
9	20876.5
10	18788.5
11	16895.0
12	18296.5
13	24174.0
14	37987.0
15	57214.5
16	73067.0
17	82600.5
18	85446.5
19	82347.0
20	76056.5
21	69525.5
22	66251.0
23	69963.5
24	81930.0
25	101215.0
26	126532.0
27	153746.5
28	182121.5
29	212916.0
30	241409.5
31	270118.0
32	300187.5
33	328188.5
34	355591.0
35	379223.0
36	404125.5
37	429384.5
38	447203.0
39	460076.0
40	474336.0
41	485775.0
42	492732.5
43	498433.0
44	500044.5
45	501772.5
46	505469.5
47	508049.0
48	502128.0
49	490105.0
50	475783.5
51	454235.5
52	427367.0
53	397610.0
54	368591.0
55	339169.5
56	308529.0
57	276578.0
58	243577.0
59	209386.5
60	171706.5
61	136436.0
62	106529.5
63	81366.0
64	60563.0
65	44839.5
66	33193.5
67	24746.0
68	18400.0
69	13155.0
70	9389.0
71	6653.5
72	4561.5
73	3038.5
74	2030.5
75	1351.0
76	871.0
77	581.0
78	400.5
79	279.5
80	204.5
81	154.5
82	127.0
83	97.5
84	68.0
85	47.0
86	29.0
87	17.5
88	9.5
89	6.0
90	5.0
91	5.0
92	5.5
93	4.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0014133178618944112
5	0.007362028636534731
6	7.984846677369555E-6
7	0.0
8	0.0
9	8.783331345106511E-4
10-11	9.302346379135532E-4
12-13	0.001273583045040444
14-15	0.0010899315714609442
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	1124.0
22-23	2193.0
24-25	3940.0
26-27	5943.0
28-29	10746.0
30-31	23497.0
32-33	30140.0
34-35	42602.0
36-37	57435.0
38-39	67394.0
40-41	74479.0
42-43	89278.0
44-45	106524.0
46-47	102893.0
48-49	92194.0
50-51	80532.0
52-53	77369.0
54-55	85767.0
56-57	90759.0
58-59	93198.0
60-61	93696.0
62-63	94336.0
64-65	101682.0
66-67	105077.0
68-69	101283.0
70-71	99265.0
72-73	96148.0
74-75	100018.0
76-77	97453.0
78-79	104778.0
80-81	108969.0
82-83	106339.0
84-85	107233.0
86-87	114379.0
88-89	121956.0
90-91	128341.0
92-93	136645.0
94-95	167007.0
96-97	424576.0
98-99	770634.0
100-101	8205669.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.32515290502104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4748959876885	71.13909291549578
2	8.265407634633846	13.443710794178514
3	2.2675533928607616	5.53227379184102
4	0.8852058861019261	2.8795801615865506
5	0.4176568244798008	1.6983002556322666
6	0.234386673145026	1.1436919219451056
7	0.13189065849085874	0.7508219577959099
8	0.0849443438146982	0.5526489399317608
9	0.0537755686468594	0.39359757084842667
>10	0.18052388763770397	2.1879391393548686
>50	0.00318017281989716	0.17102852575842564
>100	5.063477846434337E-4	0.06417219160264448
>500	5.4468805867605375E-5	0.026932029360995893
>1k	1.8153089669324206E-5	0.01620980466775871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.596969335473911E-5
2	0.0	3.193938670947822E-5	0.0	0.0	1.596969335473911E-5
3	0.0	3.992423338684778E-5	0.0	0.0	1.596969335473911E-5
4	0.0	1.0380300680580422E-4	0.0	0.0	1.596969335473911E-5
5	0.0	1.0380300680580422E-4	0.0	0.0	1.596969335473911E-5
6	7.984846677369555E-5	1.0380300680580422E-4	0.0	0.0	1.596969335473911E-5
7	7.984846677369555E-5	1.1178785348317378E-4	0.0	7.984846677369555E-6	1.596969335473911E-5
8	9.581816012843466E-5	1.2775754683791289E-4	0.0	7.984846677369555E-6	2.3954540032108666E-5
9	9.581816012843466E-5	1.5171208687002154E-4	0.0	6.387877341895644E-5	3.992423338684778E-5
10-11	9.981058346711944E-5	1.5171208687002154E-4	0.0	8.783331345106511E-5	3.992423338684778E-5
12-13	1.0380300680580422E-4	1.5570451020870634E-4	0.0	1.0380300680580422E-4	5.589392674158689E-5
14-15	1.0380300680580422E-4	2.5551509367582577E-4	0.0	1.1977270016054333E-4	7.1863620096326E-5
16-17	1.956287435955541E-4	2.7148478703056486E-4	0.0	1.2775754683791289E-4	7.984846677369555E-5
18-19	1.9962116693423887E-4	2.954393270626736E-4	0.0	1.5570451020870634E-4	1.1578027682185855E-4
20-21	1.9962116693423887E-4	3.193938670947822E-4	0.0	1.79659050240815E-4	1.7566662690213022E-4
22-23	2.115984369502932E-4	3.393559837882061E-4	0.0	1.9163632025686933E-4	1.7566662690213022E-4
24-25	2.2357570696634755E-4	3.752877938363691E-4	0.0	2.5152267033714095E-4	1.9163632025686933E-4
26-27	2.2357570696634755E-4	3.912574871911082E-4	0.0	3.553256771429452E-4	1.9163632025686933E-4
28-29	2.2357570696634755E-4	3.912574871911082E-4	0.0	8.384089011238033E-4	2.2357570696634755E-4
30-31	2.2357570696634755E-4	4.3517414391664073E-4	0.0	0.0022477343396795297	2.4753024699845623E-4
32-33	2.2756813030503232E-4	4.471514139326951E-4	0.0	0.005549468440771841	2.4753024699845623E-4
34-35	2.4753024699845623E-4	4.790908006421733E-4	0.0	0.01013676285692065	2.5551509367582577E-4
36-37	2.4753024699845623E-4	5.38977150722445E-4	0.0	0.017087571889570847	2.5551509367582577E-4
38-39	2.51522670337141E-4	5.789013841092927E-4	0.0	0.03443864371949489	2.7148478703056486E-4
40-41	2.5551509367582577E-4	6.946816609311513E-4	0.0	0.052137056379884514	2.7148478703056486E-4
42-43	2.634999403531953E-4	7.465831643340534E-4	0.0	0.06356337197520034	2.7148478703056486E-4
44-45	2.794696337079344E-4	8.024770910756403E-4	0.0	0.07739312642040441	2.9543932706267354E-4
46-47	2.9543932706267354E-4	8.463937478011728E-4	0.0	0.09240463817385919	3.034241737400431E-4
48-49	2.9543932706267354E-4	8.543785944785424E-4	0.0	0.10627431685245009	3.034241737400431E-4
50-51	3.114090204174127E-4	9.302346379135532E-4	0.0	0.11990445013071993	3.393559837882061E-4
52-53	3.114090204174127E-4	9.422119079296075E-4	0.0	0.14907309504315092	3.433484071268909E-4
54-55	3.114090204174127E-4	9.581816012843465E-4	0.0	0.18514463990816787	3.433484071268909E-4
56-57	3.114090204174127E-4	9.821361413164553E-4	0.0	0.22365555543312124	3.792802171750539E-4
58-59	3.114090204174127E-4	9.821361413164553E-4	0.0	0.2578706234456498	4.1920445056190165E-4
60-61	3.2737871377215176E-4	0.0010140755280259334	0.0	0.2715885900373707	4.3118172057795596E-4
62-63	3.433484071268909E-4	0.0010460149147354118	0.0	0.2884126619865883	4.3517414391664073E-4
64-65	3.433484071268909E-4	0.0011178785348317378	0.0	0.29530757709249694	4.3916656725532556E-4
66-67	3.433484071268909E-4	0.0011378406515251617	0.0	0.300461795622739	4.4315899059401033E-4
68-69	3.433484071268909E-4	0.0011697800382346399	0.0	0.30557209749625547	4.591286839487494E-4
70-71	3.5133325380426044E-4	0.0011777648849120095	0.0	0.3103470358093225	4.7110595396480373E-4
72-73	3.8327264051373866E-4	0.0011897421549280638	0.0	0.3140040955875577	4.7110595396480373E-4
74-75	3.8327264051373866E-4	0.0012097042716214877	0.0	0.31700240551491	4.7509837730348855E-4
76-77	3.9924233386847774E-4	0.001273583045040444	0.0	0.3200526169456652	4.790908006421733E-4
78-79	4.0722718054584734E-4	0.0013135072784272919	0.0	0.32194901803154047	4.830832239808581E-4
80-81	4.0722718054584734E-4	0.001329476971782031	0.0	0.3226197451524395	5.070377640129667E-4
82-83	4.112196038845321E-4	0.0013414542417980852	0.0	0.32316271472650065	5.269998807063906E-4
84-85	4.152120272232169E-4	0.0013614163584915093	0.0	0.3234142373968378	5.309923040450755E-4
86-87	4.1920445056190165E-4	0.0013893633218623027	0.0	0.3235180404036436	5.469619973998145E-4
88	4.231968739005864E-4	0.001453242095281259	0.0	0.3235220328269823	5.589392674158689E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18510	0.0	47.5772	1
GTATCAA	32765	0.0	35.73715	1
TCAACGC	38820	0.0	29.568567	4
ATCAACG	39120	0.0	29.31996	3
CAACGCA	39665	0.0	28.906094	5
AACGCAG	40240	0.0	28.58615	6
TATCAAC	41195	0.0	28.019304	2
ACGCAGA	45505	0.0	25.194948	7
CGCAGAG	45895	0.0	24.971834	8
GCAGAGT	53560	0.0	21.214783	9
GTACATG	36150	0.0	20.340342	1
TACATGG	35370	0.0	20.196655	2
GAGTACT	33555	0.0	19.982258	12-13
ACATGGG	35710	0.0	19.585514	3
CAGAGTA	52745	0.0	18.875954	10-11
AGAGTAC	48635	0.0	18.721964	10-11
CTTATAC	12055	0.0	17.8377	1
GTACTTT	38610	0.0	17.388369	14-15
CATGGGG	24555	0.0	17.288242	4
AGTACTT	36330	0.0	17.079252	12-13
>>END_MODULE