FastQCFastQC Report
Fri 27 May 2016
SRR2139239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12037912
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167700.050.38861
GTATCAA305250.036.7597921
TCAACGC370800.029.668974
ATCAACG373850.029.426923
CAACGCA380450.028.8950925
TATCAAC385050.028.716222
AACGCAG388250.028.3130556
ACGCAGA444350.024.7774777
CGCAGAG449100.024.4773398
GCAGAGT522950.020.996159
TGGTATC76600.020.3079552
GAGTACT323250.020.05511912-13
GTGGTAT83600.019.9992281
GTACATG348750.019.8435361
TACATGG345300.019.4178332
AGAGTAC472150.019.0658110-11
CAGAGTA509550.018.92226210-11
ACATGGG353500.018.3458773
GTACTTT365250.017.63137614-15
AGTACTT352600.017.35311912-13
Produced by FastQC (version 0.11.5)