##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12037912
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51382216450826	33.0	33.0	33.0	33.0	33.0
2	32.51436411896017	33.0	33.0	33.0	33.0	33.0
3	32.512400987812505	33.0	33.0	33.0	33.0	33.0
4	36.405587447391206	37.0	37.0	37.0	37.0	37.0
5	36.43112725861428	37.0	37.0	37.0	37.0	37.0
6	36.42125519774525	37.0	37.0	37.0	37.0	37.0
7	36.39594665586524	37.0	37.0	37.0	37.0	37.0
8	36.3836056452315	37.0	37.0	37.0	37.0	37.0
9	36.42757140939392	37.0	37.0	37.0	37.0	37.0
10-11	36.36754015148142	37.0	37.0	37.0	37.0	37.0
12-13	36.34839364168803	37.0	37.0	37.0	37.0	37.0
14-15	38.24983842712922	40.0	37.0	40.0	37.0	40.0
16-17	38.20290267116091	40.0	37.0	40.0	35.0	40.0
18-19	38.21252203039863	40.0	37.0	40.0	35.0	40.0
20-21	38.21065968876765	40.0	37.0	40.0	33.0	40.0
22-23	38.21703393222877	40.0	37.0	40.0	37.0	40.0
24-25	38.195869588688254	40.0	37.0	40.0	35.0	40.0
26-27	38.063090412151375	40.0	37.0	40.0	33.0	40.0
28-29	37.99516790087698	40.0	37.0	40.0	33.0	40.0
30-31	37.92768428360297	40.0	37.0	40.0	33.0	40.0
32-33	37.861326149599925	40.0	37.0	40.0	33.0	40.0
34-35	37.81352037874827	40.0	37.0	40.0	33.0	40.0
36-37	37.75301266002407	40.0	37.0	40.0	33.0	40.0
38-39	37.701716451074645	40.0	37.0	40.0	33.0	40.0
40-41	37.677615745841834	40.0	37.0	40.0	33.0	40.0
42-43	37.611693764000805	40.0	37.0	40.0	33.0	40.0
44-45	37.525498322565944	40.0	37.0	40.0	33.0	40.0
46-47	37.48221093747349	40.0	37.0	40.0	33.0	40.0
48-49	37.47773920444024	40.0	37.0	40.0	33.0	40.0
50-51	37.3870694265797	40.0	37.0	40.0	33.0	40.0
52-53	37.267048814102395	40.0	37.0	40.0	33.0	40.0
54-55	37.13721363714576	38.5	37.0	40.0	33.0	40.0
56-57	37.02931446412575	37.0	37.0	40.0	33.0	40.0
58-59	36.897732343807256	37.0	37.0	40.0	33.0	40.0
60-61	36.741637663936686	37.0	37.0	40.0	33.0	40.0
62-63	36.555729846303194	37.0	37.0	40.0	33.0	40.0
64-65	36.39560875897965	37.0	37.0	40.0	33.0	40.0
66-67	36.172665314918184	37.0	37.0	40.0	33.0	40.0
68-69	35.97983967301661	37.0	37.0	40.0	33.0	40.0
70-71	35.728299706815775	37.0	33.0	37.0	33.0	40.0
72-73	35.51271604554205	37.0	33.0	37.0	33.0	40.0
74-75	35.31254922053867	37.0	33.0	37.0	33.0	40.0
76-77	33.52772314080851	35.0	33.0	37.0	27.0	37.0
78-79	34.78831170736444	37.0	33.0	37.0	30.0	37.0
80-81	34.87842588173032	37.0	33.0	37.0	33.0	37.0
82-83	34.735979187465865	37.0	33.0	37.0	33.0	37.0
84-85	34.57068132753983	37.0	33.0	37.0	30.0	37.0
86-87	34.428920790673544	37.0	33.0	37.0	27.0	37.0
88-89	34.3016252812922	37.0	33.0	37.0	27.0	37.0
90-91	34.23438724155165	37.0	33.0	37.0	27.0	37.0
92-93	34.19098081099944	37.0	33.0	37.0	27.0	37.0
94-95	34.20163588462509	37.0	33.0	37.0	27.0	37.0
96-97	34.16590419472831	37.0	33.0	37.0	27.0	37.0
98-99	34.197454339067306	37.0	33.0	37.0	27.0	37.0
100	34.203619474508486	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	27.0
20	112.0
21	623.0
22	2294.0
23	6539.0
24	14636.0
25	28702.0
26	49573.0
27	79627.0
28	116535.0
29	161818.0
30	218920.0
31	289396.0
32	379541.0
33	500032.0
34	674669.0
35	948457.0
36	1576367.0
37	3377548.0
38	3526217.0
39	86273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49854227211496	20.35715994601057	14.453046342256032	23.691251439618433
2	14.545279945558665	21.26951916578224	41.777402925025534	22.40779796363356
3	17.890793685815282	26.586080709013327	30.733461085277913	24.789664519893485
4	12.280463031128315	18.466043658426404	39.3442629760016	29.909230334443677
5	13.223320312435098	37.83566751349582	35.77231483435324	13.168697339715843
6	29.377591292840698	38.38044976245877	18.979482318774604	13.26247662592593
7	25.85805793048312	32.95693081631855	23.601271017870125	17.583740235328207
8	24.09113806447497	36.972499882039344	21.33369142422706	17.60267062925863
9	25.41465373907142	16.89287466205453	21.434379910307644	36.25809168856641
10-11	23.908509392576917	26.9008824200826	29.21354260295316	19.97706558438732
12-13	24.923757627196718	24.549634913434694	29.22307855991332	21.30352889945527
14-15	22.027928473395004	25.853731497896227	26.97168619828903	25.146653830419734
16-17	21.467107418628746	28.92315959777742	27.631889151540566	21.977843832053267
18-19	21.485831596044232	27.98112330444017	29.502379648563636	21.03066545095196
20-21	22.246621360613776	27.151627683335246	29.470831161654488	21.130919794396497
22-23	22.010567310858995	27.1099769000007	29.271859009913626	21.607596779226686
24-25	21.765496437582645	27.305595057178383	29.32713680118917	21.601771704049803
26-27	21.771698482047	27.425917641205842	29.275284692824954	21.527099183922203
28-29	21.677231410411483	27.450675578422164	29.32067285816752	21.551420152998833
30-31	21.957064705583115	27.3419892962324	29.155952450234622	21.544993547949858
32-33	21.76993214154456	27.481679851763214	29.07034695883433	21.678041047857892
34-35	21.861588747460956	27.541830339032202	29.09400903761527	21.502571875891576
36-37	21.904682114092367	27.494679244263992	28.9600402815887	21.64059836005494
38-39	21.716372354016077	27.58061761186306	29.05031951249751	21.652690521623356
40-41	22.00246050141921	27.4489881854458	28.896807484596476	21.651743828538518
42-43	21.937835543493858	27.562744154464013	28.672047391733685	21.827372910308444
44-45	22.021987755229556	27.692394786730894	28.455881246905605	21.829736211133945
46-47	22.130392433776493	27.722225327936822	28.097124441414188	22.0502577968725
48-49	22.042901807715022	27.85147195684284	28.066476815575665	22.039149419866476
50-51	22.031975573176442	27.969117946703637	27.979014411189507	22.019892068930407
52-53	22.24310364125202	27.859512987978036	27.877016335840604	22.020367034929343
54-55	22.184500033947753	27.78657115736979	27.86379896186447	22.165129846817987
56-57	22.164671652400138	27.91240120027932	27.82316576976964	22.0997613775509
58-59	22.157064429436264	27.949635762982773	27.858793095417205	22.03450671216376
60-61	22.236245233006212	27.942212300384224	27.822098178594807	21.99944428801476
62-63	22.195372326378212	28.04497752315739	27.740518701566923	22.019131448897475
64-65	22.19693225866911	27.982422782699956	27.695774911217274	22.12487004741366
66-67	22.229451287939376	27.949240827512206	27.628570850811563	22.192737033736858
68-69	22.22191608382776	27.938030832086497	27.685338795798646	22.154714288287096
70-71	22.20466951171568	27.739553447720382	27.771846660275973	22.283930380287966
72-73	22.281451227821826	27.7687427685355	27.67346150867928	22.276344494963396
74-75	22.120288755065015	27.87177464214369	27.670069254119174	22.33786734867212
76-77	22.265526032583054	27.793028781153367	27.635484759477823	22.305960426785756
78-79	22.244331600082106	27.78926161425961	27.593814627117276	22.372592158541014
80-81	22.244799007301182	27.812094096417926	27.62328911208474	22.31981778419615
82-83	22.26566770731853	27.78980493383597	27.672710420554576	22.27181693829092
84-85	22.364861113690683	27.737151439075365	27.641589069428584	22.25639837780537
86-87	22.277528877764826	27.705172285931607	27.695894697746454	22.321404138557117
88-89	22.29956943010326	27.683071950807296	27.64418740929041	22.37317120979903
90-91	22.334442979180285	27.666666736132413	27.6901166357683	22.308773648919004
92-93	22.340502983698247	27.60783948506451	27.741796921835178	22.309860609402065
94-95	22.236758127506576	27.64368272705322	27.77839537070675	22.34116377473346
96-97	22.310928775397272	27.708910474741394	27.828607466140156	22.151553283721174
98-99	23.411574508694542	29.656912788113164	28.0422653965946	18.889247306597692
100	22.370903920970946	30.665146437129913	21.93994421762464	25.0240054242745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2936.0
1	2601.5
2	3619.5
3	5281.5
4	7131.0
5	11681.5
6	15766.5
7	18869.5
8	21281.0
9	21346.0
10	19254.5
11	16886.5
12	17758.5
13	22904.5
14	35356.0
15	52878.5
16	67221.0
17	75829.5
18	78315.5
19	74915.0
20	68120.0
21	61182.5
22	57520.0
23	59670.5
24	68503.5
25	84359.5
26	105420.5
27	128185.5
28	151609.0
29	178307.0
30	204308.0
31	229552.0
32	257446.0
33	283317.5
34	308167.0
35	332170.0
36	356934.0
37	380976.0
38	400771.0
39	416454.0
40	430528.0
41	444589.5
42	454242.0
43	461403.5
44	468067.5
45	476055.5
46	481405.0
47	481986.5
48	480728.0
49	473010.5
50	463128.0
51	450940.5
52	432498.5
53	410253.5
54	386556.5
55	359622.0
56	330735.0
57	300644.0
58	267542.0
59	231517.0
60	193146.5
61	157010.0
62	123848.0
63	94723.5
64	70478.0
65	51627.0
66	38136.5
67	28586.5
68	21169.5
69	14952.0
70	10536.0
71	7693.5
72	5487.0
73	3814.5
74	2683.0
75	1841.0
76	1209.5
77	740.5
78	480.5
79	344.0
80	259.0
81	197.0
82	151.5
83	117.5
84	92.0
85	68.5
86	52.5
87	40.5
88	23.5
89	11.5
90	7.0
91	4.5
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001578346809646058
5	0.007077639377991798
6	1.661417694364272E-5
7	8.30708847182136E-6
8	0.0
9	8.057875817666718E-4
10-11	8.971655549567068E-4
12-13	0.0012502168150091146
14-15	0.0010425396032135805
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	224.0
20-21	907.0
22-23	1960.0
24-25	4395.0
26-27	5676.0
28-29	9336.0
30-31	19997.0
32-33	24826.0
34-35	35346.0
36-37	47947.0
38-39	57529.0
40-41	62597.0
42-43	77320.0
44-45	93392.0
46-47	89748.0
48-49	80391.0
50-51	71494.0
52-53	69313.0
54-55	75204.0
56-57	80208.0
58-59	80754.0
60-61	80566.0
62-63	79737.0
64-65	85297.0
66-67	92226.0
68-69	91737.0
70-71	91892.0
72-73	88579.0
74-75	92098.0
76-77	89615.0
78-79	97673.0
80-81	101143.0
82-83	100051.0
84-85	101800.0
86-87	109269.0
88-89	118946.0
90-91	125664.0
92-93	134279.0
94-95	157785.0
96-97	416136.0
98-99	753964.0
100-101	8040891.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.82150208126082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.78919631289835	71.83043908828027
2	8.155487397132951	13.345884580764203
3	2.1719053969062174	5.331256858597909
4	0.8456712627451954	2.767763719390739
5	0.3993011078513589	1.6335708213554856
6	0.22216731782883506	1.0906838194872097
7	0.11844917018506583	0.6784182317374687
8	0.07837635783395729	0.5130297060506223
9	0.04842908432975845	0.35662863818527973
>10	0.16651580430535912	2.111286046224886
>50	0.0035516489781045628	0.19567585365040802
>100	9.111657346228086E-4	0.12028890578328486
>500	3.797327014785332E-5	0.025073730492244414
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	8.30708847182136E-6	0.0	1.661417694364272E-5	0.0
4	0.0	1.661417694364272E-5	0.0	1.661417694364272E-5	0.0
5	0.0	2.4921265415464076E-5	0.0	1.661417694364272E-5	0.0
6	5.814961930274951E-5	2.4921265415464076E-5	0.0	1.661417694364272E-5	0.0
7	5.814961930274951E-5	2.4921265415464076E-5	0.0	3.322835388728544E-5	0.0
8	5.814961930274951E-5	6.645670777457087E-5	0.0	4.15354423591068E-5	8.30708847182136E-6
9	5.814961930274951E-5	1.412205040209631E-4	0.0	9.96850616618563E-5	8.30708847182136E-6
10-11	5.814961930274951E-5	1.412205040209631E-4	0.0	1.2875987131323106E-4	8.30708847182136E-6
12-13	5.814961930274951E-5	1.412205040209631E-4	0.0	1.4952759249278446E-4	8.30708847182136E-6
14-15	5.814961930274951E-5	1.9106303485189125E-4	0.0	1.4952759249278446E-4	4.15354423591068E-5
16-17	8.722442895412427E-5	1.9521657908780192E-4	0.0	1.4952759249278446E-4	4.984253083092815E-5
18-19	9.137797319003495E-5	2.242913887391767E-4	0.0	1.4952759249278446E-4	5.814961930274951E-5
20-21	9.137797319003495E-5	2.492126541546408E-4	0.0	1.578346809646058E-4	6.23031635386602E-5
22-23	9.137797319003495E-5	2.907480965137476E-4	0.0	1.661417694364272E-4	8.30708847182136E-5
24-25	9.137797319003495E-5	3.1151581769330093E-4	0.0	1.869094906159806E-4	9.96850616618563E-5
26-27	9.137797319003495E-5	3.364370831087651E-4	0.0	2.1598430026735534E-4	1.03838605897767E-4
28-29	9.553151742594563E-5	3.405906273446757E-4	0.0	3.862796139396932E-4	1.0799215013367767E-4
30-31	1.03838605897767E-4	3.862796139396932E-4	0.0	9.013190991926175E-4	1.0799215013367767E-4
32-33	1.0799215013367767E-4	4.112008793551573E-4	0.0	0.0018192523753288777	1.412205040209631E-4
34-35	1.0799215013367767E-4	4.651969544219961E-4	0.0	0.0031151581769330097	1.4952759249278446E-4
36-37	1.0799215013367767E-4	5.399607506683884E-4	0.0	0.005171162573708796	1.4952759249278446E-4
38-39	1.0799215013367767E-4	5.399607506683884E-4	0.0	0.008340316825708644	1.661417694364272E-4
40-41	1.0799215013367767E-4	6.313387238584232E-4	0.0	0.011484549812293028	1.827559463800699E-4
42-43	1.1214569436958835E-4	6.645670777457088E-4	0.0	0.014595554444990128	1.827559463800699E-4
44-45	1.1629923860549903E-4	7.64252139407565E-4	0.0	0.018217445018704242	1.827559463800699E-4
46-47	1.1629923860549903E-4	8.431694798898679E-4	0.0	0.022092701790808904	2.07677211795534E-4
48-49	1.1629923860549903E-4	8.514765683616893E-4	0.0	0.02603856881492405	2.492126541546408E-4
50-51	1.3291341554914175E-4	9.636222627312776E-4	0.0	0.029913825587028715	2.492126541546408E-4
52-53	1.453740482568738E-4	9.926970723826525E-4	0.0	0.03350248780685554	2.949016407496583E-4
54-55	1.4952759249278446E-4	0.0010051577050903845	0.0	0.037049614584323265	3.405906273446757E-4
56-57	1.4952759249278446E-4	0.001013464793562206	0.0	0.041348532868490814	3.405906273446757E-4
58-59	1.4952759249278446E-4	0.0010466931474494912	0.0	0.04593819924917211	3.447441715805864E-4
60-61	1.4952759249278446E-4	0.0010674608686290447	0.0	0.05027865297569878	3.488977158164971E-4
62-63	1.827559463800699E-4	0.001088228589808598	0.0	0.054714638219651376	3.738189812319612E-4
64-65	1.827559463800699E-4	0.0011962207399422756	0.0	0.05889725726521343	3.738189812319612E-4
66-67	1.869094906159806E-4	0.0012086813726500077	0.0	0.06329586061104284	3.738189812319612E-4
68-69	1.9106303485189125E-4	0.0012751380804245788	0.0	0.0680225939515092	3.738189812319612E-4
70-71	1.9521657908780192E-4	0.001295905801604132	0.0	0.07230905160296902	3.7797252546787187E-4
72-73	2.1598430026735534E-4	0.0013000593458400427	0.0	0.07559450509357438	4.319686005347107E-4
74-75	2.1598430026735534E-4	0.001337441243963239	0.0	0.07823200568337765	4.319686005347107E-4
76-77	2.3259847721099805E-4	0.0013955908632659884	0.0	0.0809733448790787	4.361221447706213E-4
78-79	2.409055656828194E-4	0.0014288192171532737	0.0	0.08288812877183352	4.40275689006532E-4
80-81	2.409055656828194E-4	0.0014288192171532737	0.0	0.08349039268604057	4.527363217142641E-4
82-83	2.409055656828194E-4	0.0014412798498610057	0.0	0.08386421166727254	4.818111313656388E-4
84-85	2.409055656828194E-4	0.0014578940268046485	0.0	0.08406773533483215	4.818111313656388E-4
86-87	2.409055656828194E-4	0.001478661747984202	0.0	0.0841258849541349	4.818111313656388E-4
88	2.409055656828194E-4	0.0015451184557587728	0.0	0.08413419204260672	4.818111313656388E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16770	0.0	50.3886	1
GTATCAA	30525	0.0	36.759792	1
TCAACGC	37080	0.0	29.66897	4
ATCAACG	37385	0.0	29.42692	3
CAACGCA	38045	0.0	28.895092	5
TATCAAC	38505	0.0	28.71622	2
AACGCAG	38825	0.0	28.313055	6
ACGCAGA	44435	0.0	24.777477	7
CGCAGAG	44910	0.0	24.477339	8
GCAGAGT	52295	0.0	20.99615	9
TGGTATC	7660	0.0	20.307955	2
GAGTACT	32325	0.0	20.055119	12-13
GTGGTAT	8360	0.0	19.999228	1
GTACATG	34875	0.0	19.843536	1
TACATGG	34530	0.0	19.417833	2
AGAGTAC	47215	0.0	19.06581	10-11
CAGAGTA	50955	0.0	18.922262	10-11
ACATGGG	35350	0.0	18.345877	3
GTACTTT	36525	0.0	17.631376	14-15
AGTACTT	35260	0.0	17.353119	12-13
>>END_MODULE