FastQCFastQC Report
Fri 27 May 2016
SRR2139238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11532441
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161850.054.772031
GTATCAA301400.037.9724271
TCAACGC376100.029.99674
ATCAACG381000.029.6109163
TATCAAC388150.029.2202992
CAACGCA386400.029.2193185
AACGCAG393950.028.6577196
ACGCAGA448400.025.178427
CGCAGAG490150.023.0514858
GTACATG391300.022.3806021
GTGGTAT73350.022.0644281
TGGTATC69400.021.9593932
TACATGG391150.021.8184812
GCAGAGT527300.021.34639
ACATGGG392750.021.1612153
GAGTACT309700.020.4382512-13
CAGAGTA516250.019.31687410-11
AGAGTAC482750.019.12783410-11
CATGGGG304750.019.059244
GTACTTT348150.018.07013714-15
Produced by FastQC (version 0.11.5)