##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11532441
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52109427657163	33.0	33.0	33.0	33.0	33.0
2	32.517473360583416	33.0	33.0	33.0	33.0	33.0
3	32.516947539553854	33.0	33.0	33.0	33.0	33.0
4	36.4143623193043	37.0	37.0	37.0	37.0	37.0
5	36.43795992539654	37.0	37.0	37.0	37.0	37.0
6	36.43028973657875	37.0	37.0	37.0	37.0	37.0
7	36.402923717537334	37.0	37.0	37.0	37.0	37.0
8	36.39056284788277	37.0	37.0	37.0	37.0	37.0
9	36.43409144690183	37.0	37.0	37.0	37.0	37.0
10-11	36.373431479077155	37.0	37.0	37.0	37.0	37.0
12-13	36.35159824359821	37.0	37.0	37.0	37.0	37.0
14-15	38.2602642406755	40.0	37.0	40.0	37.0	40.0
16-17	38.21383508487058	40.0	37.0	40.0	37.0	40.0
18-19	38.22410346603984	40.0	37.0	40.0	37.0	40.0
20-21	38.21824312932873	40.0	37.0	40.0	35.0	40.0
22-23	38.22434521062079	40.0	37.0	40.0	37.0	40.0
24-25	38.20200972382367	40.0	37.0	40.0	35.0	40.0
26-27	38.06809211394769	40.0	37.0	40.0	33.0	40.0
28-29	37.99871678256994	40.0	37.0	40.0	33.0	40.0
30-31	37.932740793652926	40.0	37.0	40.0	33.0	40.0
32-33	37.86271812124583	40.0	37.0	40.0	33.0	40.0
34-35	37.81069831552806	40.0	37.0	40.0	33.0	40.0
36-37	37.7464925741461	40.0	37.0	40.0	33.0	40.0
38-39	37.68710631527535	40.0	37.0	40.0	33.0	40.0
40-41	37.65362879129057	40.0	37.0	40.0	33.0	40.0
42-43	37.58338261035362	40.0	37.0	40.0	33.0	40.0
44-45	37.486220765047165	40.0	37.0	40.0	33.0	40.0
46-47	37.43394760330624	40.0	37.0	40.0	33.0	40.0
48-49	37.41576688071386	40.0	37.0	40.0	33.0	40.0
50-51	37.310657677339776	40.0	37.0	40.0	33.0	40.0
52-53	37.17435639119252	40.0	37.0	40.0	33.0	40.0
54-55	37.0325511363645	37.0	37.0	40.0	33.0	40.0
56-57	36.91362566141019	37.0	37.0	40.0	33.0	40.0
58-59	36.758736784049525	37.0	37.0	40.0	33.0	40.0
60-61	36.5919202584147	37.0	37.0	40.0	33.0	40.0
62-63	36.400702367772496	37.0	37.0	40.0	33.0	40.0
64-65	36.225574050573805	37.0	37.0	40.0	33.0	40.0
66-67	35.98921798569238	37.0	35.0	40.0	33.0	40.0
68-69	35.79448024745639	37.0	33.0	38.5	33.0	40.0
70-71	35.54536325514997	37.0	33.0	37.0	33.0	40.0
72-73	35.342904440744284	37.0	33.0	37.0	33.0	40.0
74-75	35.154638446160085	37.0	33.0	37.0	33.0	40.0
76-77	33.335431118676894	35.0	33.0	37.0	27.0	37.0
78-79	34.653660261236595	37.0	33.0	37.0	30.0	37.0
80-81	34.76799389562024	37.0	33.0	37.0	33.0	37.0
82-83	34.63446831120841	37.0	33.0	37.0	30.0	37.0
84-85	34.479062696586375	37.0	33.0	37.0	27.0	37.0
86-87	34.35396303229216	37.0	33.0	37.0	27.0	37.0
88-89	34.23775849656208	37.0	33.0	37.0	27.0	37.0
90-91	34.177388517708664	37.0	33.0	37.0	27.0	37.0
92-93	34.129867214593546	37.0	33.0	37.0	27.0	37.0
94-95	34.146245905150224	37.0	33.0	37.0	27.0	37.0
96-97	34.1190368933611	37.0	33.0	37.0	27.0	37.0
98-99	34.15110598748426	37.0	33.0	37.0	27.0	37.0
100	34.15661829841985	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	3.0
18	4.0
19	18.0
20	126.0
21	615.0
22	2256.0
23	6425.0
24	14468.0
25	28365.0
26	49374.0
27	77577.0
28	114065.0
29	158937.0
30	213908.0
31	279889.0
32	368996.0
33	485891.0
34	655746.0
35	933584.0
36	1584481.0
37	3326104.0
38	3154157.0
39	77450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.122955582430464	19.805182614851443	14.160870192182209	23.910991610535877
2	15.052329337735177	21.047677590546527	40.913272393936374	22.986720677781918
3	18.190546129826288	26.11449735576362	30.195671497473953	25.499285016936135
4	12.66118465835434	18.040394475675647	38.228726868170796	31.06969399779922
5	13.774756333549984	37.37568230725105	35.16309251628888	13.686468842910097
6	30.36825421435063	37.39253467674363	18.55073873779194	13.6884723711138
7	26.82446445196892	32.454088853190555	22.96907011604397	17.752376578796557
8	24.960040983517715	36.14605095313299	20.68119836901832	18.212709694330975
9	26.04547886046093	16.381799819238875	20.894315788451387	36.67840553184881
10-11	24.652586573220415	26.44333863567161	28.362663982965806	20.541410808142167
12-13	25.522519721144764	24.200596897823953	28.36620422028856	21.91067916074272
14-15	22.625782578434738	25.20928481764344	26.29368590101697	25.871246702904855
16-17	22.107574623620447	28.372813700065752	26.830815783059286	22.688795893254515
18-19	22.1906576413441	27.40796159286659	28.64437372799046	21.75700703779885
20-21	23.161059652254586	26.459529648231445	28.621291562017902	21.758119137496063
22-23	22.92187858684249	26.428234878251484	28.378714022953538	22.271172511952493
24-25	22.63569213949007	26.590439118800973	28.5837170213303	22.190151720378648
26-27	22.489271801493455	26.67601675727143	28.533973044404686	22.30073839683043
28-29	22.55212672162197	26.733989735058945	28.48598414026235	22.227899403056735
30-31	22.839816120336202	26.442872160057647	28.433393985458178	22.283917734147977
32-33	22.623148451356847	26.60375546475838	28.30765517623881	22.465440907645963
34-35	22.781156172454715	26.70659356795643	28.224630384518797	22.287619875070067
36-37	22.89421179010027	26.628540757667167	28.05314145761083	22.424105994621733
38-39	22.532485978966758	26.92695567119772	28.153677861587116	22.38688048824841
40-41	22.745010986464692	26.676657443843887	28.118477822995207	22.45985374669621
42-43	22.614628516973408	26.871230996071244	27.797205192344748	22.716935294610604
44-45	22.65253672706445	26.929124647821517	27.64847517613686	22.769863448977173
46-47	22.813688642624818	26.921415149676303	27.44004214471542	22.82485406298346
48-49	22.90607735713074	27.17339914705519	27.155848598421663	22.76467489739241
50-51	22.88530124280587	27.21152792501784	27.12594824756995	22.777222584606335
52-53	23.11904922757041	27.102357883611596	26.999047811718285	22.779545077099712
54-55	23.122710842081716	26.98340193781356	26.994137643939514	22.899749576165206
56-57	23.094631794542348	27.21924862102511	26.831085438253805	22.855034146178742
58-59	22.924099905754634	27.216522264736387	27.008081573321707	22.851296256187272
60-61	23.051986743672224	27.173792611247578	26.90809708195901	22.866123563121192
62-63	23.081167603826692	27.21865617474386	26.96259774220668	22.737578479222773
64-65	23.139461345145424	27.081223134210404	26.889044458039034	22.890271062605137
66-67	23.174723523864994	27.18146303921896	26.70861340347377	22.93520003344228
68-69	23.10422071703778	27.10509358505911	26.811535425778292	22.979150272124816
70-71	23.10849494400905	26.967800649641326	26.809056992019553	23.114647414330076
72-73	23.112158954838133	27.015609375679045	26.691735007711458	23.18049666177136
74-75	22.977961858241798	27.0583597525331	26.75620254843879	23.20747584078631
76-77	23.134635587701144	27.056697372650333	26.66579056217198	23.14287647747654
78-79	22.97826743311804	27.084565071963567	26.722751153061797	23.2144163418566
80-81	23.083083948254377	27.017647758124085	26.775735650880232	23.123532642741303
82-83	23.211418719597017	26.894548977518134	26.917387341597603	22.97664496128724
84-85	23.14884472540871	26.9301744837386	26.824898732161333	23.09608205869135
86-87	23.031967316447634	27.035398591195086	26.824640534400046	23.107993557957233
88-89	23.200466847041035	26.824133362203373	26.740788776979745	23.234611013775847
90-91	23.16966432452712	26.862311977171505	26.784266349353413	23.183757348947964
92-93	23.21070586571908	26.853644292020718	26.91545192498709	23.020197917273112
94-95	22.977729407547407	26.992996856501687	26.922651491016886	23.106622244934016
96-97	23.036736736568823	27.029548677857747	26.98790157424316	22.94581301133027
98-99	24.269890446322396	28.967047904228505	27.098892494678584	19.664169154770512
100	23.061157734346747	30.04518116964765	20.979796822512192	25.91386427349341
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2968.0
1	2587.5
2	3352.5
3	4788.0
4	6461.0
5	10504.0
6	14083.0
7	16844.5
8	19031.5
9	19138.0
10	17507.0
11	15805.0
12	16951.0
13	22025.0
14	34086.0
15	51089.0
16	65224.0
17	73554.5
18	75507.0
19	71173.0
20	63216.5
21	54991.0
22	49731.0
23	50383.5
24	56881.0
25	69424.5
26	86323.0
27	103798.0
28	123178.5
29	145903.0
30	167212.5
31	187553.0
32	209605.5
33	230634.0
34	252289.5
35	272878.0
36	295766.5
37	318171.5
38	333954.0
39	349862.0
40	366870.5
41	380497.5
42	389820.0
43	399241.5
44	410268.5
45	428450.5
46	446386.5
47	464562.0
48	477140.5
49	476265.5
50	475574.5
51	475865.0
52	458131.5
53	429044.5
54	409847.5
55	392762.0
56	372817.0
57	343778.0
58	310783.0
59	275413.5
60	234856.5
61	201207.5
62	166427.0
63	134702.0
64	112010.0
65	88730.5
66	70644.0
67	52584.0
68	34637.5
69	25313.5
70	18537.5
71	11845.5
72	8048.5
73	5846.5
74	4357.0
75	3116.5
76	2050.5
77	1203.5
78	881.5
79	702.5
80	489.5
81	327.5
82	255.5
83	176.0
84	106.5
85	76.0
86	61.5
87	53.5
88	43.5
89	33.5
90	22.0
91	13.5
92	11.5
93	6.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001482773681651612
5	0.006902268132132651
6	0.0
7	1.7342382241539325E-5
8	0.0
9	8.150919653523482E-4
10-11	9.321530454827386E-4
12-13	0.0012920074769946796
14-15	0.001131590441260441
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	244.0
20-21	935.0
22-23	1882.0
24-25	4142.0
26-27	5491.0
28-29	8963.0
30-31	19862.0
32-33	24939.0
34-35	35669.0
36-37	49223.0
38-39	58772.0
40-41	63229.0
42-43	79445.0
44-45	95272.0
46-47	92194.0
48-49	82117.0
50-51	72403.0
52-53	69941.0
54-55	75407.0
56-57	80563.0
58-59	81387.0
60-61	80411.0
62-63	78702.0
64-65	85986.0
66-67	91002.0
68-69	93547.0
70-71	92256.0
72-73	88778.0
74-75	92954.0
76-77	90115.0
78-79	96929.0
80-81	100040.0
82-83	98842.0
84-85	101601.0
86-87	108381.0
88-89	117103.0
90-91	124231.0
92-93	130847.0
94-95	152760.0
96-97	403967.0
98-99	717112.0
100-101	7584797.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.50432840434388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.8029447876061	73.26627320029472
2	7.539024835255198	12.440043617127989
3	1.9520126514772105	4.831484785407296
4	0.7443933814516569	2.456627040212301
5	0.3423695109368674	1.4123483282984957
6	0.188277356747726	0.9320218123329718
7	0.11497792777007544	0.6640323698395264
8	0.07297383198166207	0.48165255989907685
9	0.05186359933165031	0.38510742883408083
>10	0.18243765114594032	2.4781396204012016
>50	0.00670812458232487	0.36978702343393727
>100	0.001973627721316689	0.25261557798741574
>500	2.1357064117403944E-5	0.011717634040771245
>1k	2.135692820670545E-5	0.018149001890282422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7342382241539325E-5	0.0	0.0	0.0
3	0.0	1.7342382241539325E-5	0.0	8.671191120769662E-6	0.0
4	0.0	4.335595560384831E-5	0.0	1.7342382241539325E-5	8.671191120769662E-6
5	0.0	7.804072008692695E-5	0.0	1.7342382241539325E-5	1.7342382241539325E-5
6	0.0	7.804072008692695E-5	0.0	2.6013573362308986E-5	1.7342382241539325E-5
7	0.0	8.671191120769662E-5	0.0	2.6013573362308986E-5	2.6013573362308986E-5
8	0.0	8.671191120769662E-5	0.0	2.6013573362308986E-5	2.6013573362308986E-5
9	0.0	1.9943739577770223E-4	0.0	4.335595560384831E-5	3.468476448307865E-5
10-11	0.0	2.0810858689847189E-4	0.0	6.503393340577246E-5	3.468476448307865E-5
12-13	8.671191120769662E-6	2.0810858689847189E-4	0.0	7.804072008692695E-5	4.335595560384831E-5
14-15	8.671191120769662E-6	3.33840858149632E-4	0.0	7.804072008692695E-5	5.202714672461797E-5
16-17	1.7342382241539325E-5	3.381764537100168E-4	0.0	7.804072008692695E-5	5.202714672461797E-5
18-19	2.6013573362308986E-5	3.59854431511941E-4	0.0	7.804072008692695E-5	5.6362742285002805E-5
20-21	2.6013573362308986E-5	4.075459826761741E-4	0.0	7.804072008692695E-5	6.069833784538763E-5
22-23	2.6013573362308986E-5	4.5090193828002244E-4	0.0	8.671191120769662E-5	6.503393340577247E-5
24-25	6.93695289661573E-5	5.029290850046404E-4	0.0	1.3873905793231457E-4	7.370512452654213E-5
26-27	9.538310232846628E-5	5.289426583669494E-4	0.0	2.688069247438595E-4	8.237631564731178E-5
28-29	9.971869788885112E-5	5.419494450481039E-4	0.0	7.543936275069606E-4	8.671191120769662E-5
30-31	1.1272548457000561E-4	6.329969518161854E-4	0.0	0.0019380112154920195	8.671191120769662E-5
32-33	1.2139667569077526E-4	6.546749296181095E-4	0.0	0.004188185311331747	9.104750676808145E-5
34-35	1.2139667569077526E-4	7.847427964296544E-4	0.0	0.007101705527910353	1.0405429344923594E-4
36-37	1.2139667569077526E-4	9.278174499223538E-4	0.0	0.011654080866314425	1.0405429344923594E-4
38-39	1.2139667569077526E-4	9.798445966469717E-4	0.0	0.019822342902079446	1.2573227125116008E-4
40-41	1.2139667569077526E-4	0.0010492141256131292	0.0	0.027435648706115208	1.387390579323146E-4
42-43	1.2139667569077526E-4	0.001083898890096208	0.0	0.03321499758810819	1.387390579323146E-4
44-45	1.2139667569077526E-4	0.0011836175879850587	0.0	0.03974440450204775	1.387390579323146E-4
46-47	1.2139667569077526E-4	0.0012573227125116009	0.0	0.04599199770456228	1.387390579323146E-4
48-49	1.2139667569077526E-4	0.0012616583080719858	0.0	0.05182337373327989	1.4741024905308425E-4
50-51	1.2139667569077526E-4	0.0014177397482458397	0.0	0.05657518646746166	1.560814401738539E-4
52-53	1.2139667569077526E-4	0.0014307465349269943	0.0	0.06066798867646494	1.7342382241539324E-4
54-55	1.2139667569077526E-4	0.0014524245127289183	0.0	0.06475211969434745	1.9076620465693255E-4
56-57	1.2139667569077526E-4	0.0014827736816516123	0.0	0.06876254558770342	2.0810858689847189E-4
58-59	1.2139667569077526E-4	0.0015087872550139212	0.0	0.07309380555252787	2.2545096914001122E-4
60-61	1.3006786681154492E-4	0.0015304652328158453	0.0	0.0774033875395504	2.3845775582116572E-4
62-63	1.3006786681154492E-4	0.0015694855928593088	0.0	0.0817433186954956	2.4279335138155053E-4
64-65	1.3006786681154492E-4	0.0018036077531200898	0.0	0.08605723627807851	2.4279335138155053E-4
66-67	1.3006786681154492E-4	0.0018036077531200898	0.0	0.09030611992725565	2.4712894694193534E-4
68-69	1.3006786681154492E-4	0.0018643060909654774	0.0	0.09487583764790125	2.55800138062705E-4
70-71	1.3006786681154492E-4	0.0019119976421297104	0.0	0.09915507046600108	2.688069247438595E-4
72-73	1.3006786681154492E-4	0.0019380112154920195	0.0	0.10252816381198049	2.861493069853988E-4
74-75	1.3006786681154492E-4	0.0019900383622166373	0.0	0.10542867724187793	2.861493069853988E-4
76-77	1.3006786681154492E-4	0.0020724146778639493	0.0	0.1086413535521231	2.904849025457837E-4
78-79	1.3006786681154492E-4	0.0020984282512262584	0.0	0.11044496130524319	2.948204981061685E-4
80-81	1.3006786681154492E-4	0.0021201062290281823	0.0	0.11109096504374052	3.1216288034770787E-4
82-83	1.3006786681154492E-4	0.0021417842068301067	0.0	0.11182368069344556	3.251696670288623E-4
84-85	1.3006786681154492E-4	0.0021721333757528	0.0	0.11210115880931018	3.33840858149632E-4
86-87	1.3006786681154492E-4	0.0022111537357962638	0.0	0.11217919952939712	3.4251204927040167E-4
88	1.3006786681154492E-4	0.002332550411487039	0.0	0.11218787072051789	3.468476448307865E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16185	0.0	54.77203	1
GTATCAA	30140	0.0	37.972427	1
TCAACGC	37610	0.0	29.9967	4
ATCAACG	38100	0.0	29.610916	3
TATCAAC	38815	0.0	29.220299	2
CAACGCA	38640	0.0	29.219318	5
AACGCAG	39395	0.0	28.657719	6
ACGCAGA	44840	0.0	25.17842	7
CGCAGAG	49015	0.0	23.051485	8
GTACATG	39130	0.0	22.380602	1
GTGGTAT	7335	0.0	22.064428	1
TGGTATC	6940	0.0	21.959393	2
TACATGG	39115	0.0	21.818481	2
GCAGAGT	52730	0.0	21.3463	9
ACATGGG	39275	0.0	21.161215	3
GAGTACT	30970	0.0	20.43825	12-13
CAGAGTA	51625	0.0	19.316874	10-11
AGAGTAC	48275	0.0	19.127834	10-11
CATGGGG	30475	0.0	19.05924	4
GTACTTT	34815	0.0	18.070137	14-15
>>END_MODULE