FastQCFastQC Report
Fri 27 May 2016
SRR2139237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10683457
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155800.053.1714021
GTATCAA286850.037.694341
TCAACGC349550.030.4062044
ATCAACG353800.030.0526433
TATCAAC361200.029.6623782
CAACGCA358950.029.611745
AACGCAG363600.029.3155576
ACGCAGA412400.025.7530637
CGCAGAG439150.024.1844778
GCAGAGT481500.021.8260159
GTACATG341300.021.6478461
GTGGTAT72450.021.0583971
TACATGG344350.021.0080662
TGGTATC68250.020.5960942
ACATGGG345300.020.4289933
GAGTACT297150.020.32829712-13
CAGAGTA472800.019.67113910-11
AGAGTAC441650.018.98667110-11
CATGGGG253400.018.773114
GTACTTT332650.018.15969514-15
Produced by FastQC (version 0.11.5)