##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10683457
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53347189023179	33.0	33.0	33.0	33.0	33.0
2	32.54418902046407	33.0	33.0	33.0	33.0	33.0
3	32.53641054576248	33.0	33.0	33.0	33.0	33.0
4	36.44644369327269	37.0	37.0	37.0	37.0	37.0
5	36.48379864307967	37.0	37.0	37.0	37.0	37.0
6	36.48553019869879	37.0	37.0	37.0	37.0	37.0
7	36.465956103909065	37.0	37.0	37.0	37.0	37.0
8	36.46037981900428	37.0	37.0	37.0	37.0	37.0
9	36.48324067761961	37.0	37.0	37.0	37.0	37.0
10-11	36.4332326137504	37.0	37.0	37.0	37.0	37.0
12-13	36.41386636366862	37.0	37.0	37.0	37.0	37.0
14-15	38.35794027158063	40.0	37.0	40.0	37.0	40.0
16-17	38.32805209961532	40.0	37.0	40.0	37.0	40.0
18-19	38.36150531611631	40.0	37.0	40.0	37.0	40.0
20-21	38.35933221383823	40.0	37.0	40.0	37.0	40.0
22-23	38.35901987227296	40.0	37.0	40.0	37.0	40.0
24-25	38.33153943882305	40.0	37.0	40.0	37.0	40.0
26-27	38.20931414213169	40.0	37.0	40.0	37.0	40.0
28-29	38.153057675481094	40.0	37.0	40.0	35.0	40.0
30-31	38.07301962627818	40.0	37.0	40.0	33.0	40.0
32-33	37.99356630644152	40.0	37.0	40.0	33.0	40.0
34-35	37.970412927598105	40.0	37.0	40.0	33.0	40.0
36-37	37.90915839423866	40.0	37.0	40.0	33.0	40.0
38-39	37.857698562987466	40.0	37.0	40.0	33.0	40.0
40-41	37.82235832381481	40.0	37.0	40.0	33.0	40.0
42-43	37.76283808192234	40.0	37.0	40.0	33.0	40.0
44-45	37.686468487894984	40.0	37.0	40.0	33.0	40.0
46-47	37.637712725564676	40.0	37.0	40.0	33.0	40.0
48-49	37.610219460643535	40.0	37.0	40.0	33.0	40.0
50-51	37.53114332181924	40.0	37.0	40.0	33.0	40.0
52-53	37.4215754313595	40.0	37.0	40.0	33.0	40.0
54-55	37.23983747126715	40.0	37.0	40.0	33.0	40.0
56-57	37.125151330051736	37.0	37.0	40.0	33.0	40.0
58-59	36.99404384067742	37.0	37.0	40.0	33.0	40.0
60-61	36.85888617914053	37.0	37.0	40.0	33.0	40.0
62-63	36.69832459036243	37.0	37.0	40.0	33.0	40.0
64-65	36.502656872338605	37.0	37.0	40.0	33.0	40.0
66-67	36.29005502709984	37.0	37.0	40.0	33.0	40.0
68-69	36.07621837732361	37.0	37.0	40.0	33.0	40.0
70-71	35.819734150060135	37.0	35.0	37.0	33.0	40.0
72-73	35.63118211871775	37.0	33.0	37.0	33.0	40.0
74-75	35.44373207803258	37.0	33.0	37.0	33.0	40.0
76-77	33.607144510664384	35.0	33.0	37.0	27.0	37.0
78-79	34.92794500230917	37.0	33.0	37.0	33.0	37.0
80-81	34.99664397252777	37.0	33.0	37.0	33.0	37.0
82-83	34.86434701437953	37.0	33.0	37.0	33.0	37.0
84-85	34.719883741339046	37.0	33.0	37.0	33.0	37.0
86-87	34.62364147247462	37.0	33.0	37.0	33.0	37.0
88-89	34.50891272214001	37.0	33.0	37.0	33.0	37.0
90-91	34.4517555155975	37.0	33.0	37.0	33.0	37.0
92-93	34.38483257244464	37.0	33.0	37.0	33.0	37.0
94-95	34.403453620238935	37.0	33.0	37.0	33.0	37.0
96-97	34.39307651848376	37.0	33.0	37.0	33.0	37.0
98-99	34.39079469627664	37.0	33.0	37.0	33.0	37.0
100	34.37294391680492	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	2.0
18	2.0
19	25.0
20	103.0
21	479.0
22	1736.0
23	4908.0
24	11682.0
25	22512.0
26	39850.0
27	63753.0
28	92466.0
29	130584.0
30	176865.0
31	232784.0
32	307496.0
33	407273.0
34	553535.0
35	794124.0
36	1368000.0
37	3058198.0
38	3332542.0
39	84535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00846224213754	20.058301353204307	14.201320789703184	23.73191561495497
2	14.789884959521999	21.277410486137587	41.364092166047	22.568612388293417
3	18.007635543438795	26.44310732003695	30.52822695874566	25.0210301777786
4	12.392560408838856	18.34508700424746	38.90797522008355	30.354377366830132
5	13.443831898232942	37.59562096800689	35.51770742372998	13.442839710030189
6	29.624454084707217	38.00507402395147	18.876505438232687	13.493966453108616
7	26.20070450978555	32.76472213067362	23.39638751763591	17.638185841904917
8	24.514592982402604	36.43422723562233	21.096832233236864	17.954347548738202
9	25.703505897014423	16.638574948165186	21.272646110711168	36.385273044109226
10-11	24.15979782503531	26.766096378703256	28.80073563440464	20.273370161856793
12-13	25.15841907458465	24.518279509201683	28.780806465026775	21.54249495118689
14-15	22.271878624814946	25.611210726569233	26.723352625742	25.393558022873826
16-17	21.72161595258913	28.71600924681964	27.255175922924575	22.30719887766666
18-19	21.731771841268234	27.749795782395154	29.14816337071418	21.37026900562243
20-21	22.646107334142307	26.822265435868708	29.151360750134568	21.380266479854413
22-23	22.369771511394003	26.824589024056028	28.919703324528058	21.88593614002191
24-25	22.134920461398377	27.009528264772825	29.043311541471727	21.81223973235707
26-27	22.032353421361712	27.09265347493033	28.995449337098904	21.879543766609057
28-29	22.045308908293404	27.104474870310508	29.019968936844894	21.8302472845512
30-31	22.33806765274736	26.872853571661448	28.886777415058784	21.902301360532412
32-33	22.177625144761905	27.017040181877494	28.82212439910385	21.983210274256752
34-35	22.304948279017207	27.151833928972252	28.739516481793387	21.803701310217154
36-37	22.371663199206676	27.047724609112265	28.59838160613541	21.982230585545643
38-39	22.081095744114805	27.324859948768232	28.69093429563922	21.90311001147774
40-41	22.312253128672708	27.095714214887924	28.60653401994238	21.985498636496985
42-43	22.20564030157301	27.246489185282247	28.334311125791743	22.213559387352998
44-45	22.219386426023295	27.34378797941338	28.141289082904482	22.29553651165885
46-47	22.391716353621227	27.320199523147863	27.87179395626819	22.416290166962725
48-49	22.466562269649085	27.550489177551864	27.64725195389785	22.3356965989012
50-51	22.44022924469565	27.59899901934302	27.60106314252036	22.35970859344097
52-53	22.624182367661664	27.520421391266876	27.522124609870534	22.333271631200926
54-55	22.651624428467443	27.412336931720322	27.492452690241613	22.44358594957062
56-57	22.675688440106843	27.656311019069012	27.24439613028067	22.423604410543476
58-59	22.476696087309264	27.666062918917962	27.484713981969854	22.37252701180292
60-61	22.60767756279593	27.669754700380413	27.353335975128918	22.369231761694746
62-63	22.588002827664898	27.660978177765195	27.42561763416181	22.325401360408097
64-65	22.642653566078565	27.556058552635943	27.35947553898621	22.44181234229928
66-67	22.73212410309498	27.598989543532703	27.167889067560296	22.500997285812023
68-69	22.67983343572011	27.49714536596323	27.2980123068336	22.525008891483065
70-71	22.662113905193177	27.38893587295604	27.29105468562578	22.657895536225002
72-73	22.689616101001224	27.420441891177965	27.186555119862703	22.703386887958104
74-75	22.575980592885767	27.459857028678456	27.22278620393347	22.74137617450231
76-77	22.664062450029544	27.462648841655877	27.152156551194338	22.721132157120238
78-79	22.532562576244626	27.475713026366172	27.230138553734097	22.761585843655112
80-81	22.61353050542682	27.414703886856906	27.253831093629742	22.71793451408653
82-83	22.760560820385827	27.283145001661328	27.379746511911897	22.576547666040945
84-85	22.760533215080315	27.301132452850997	27.297729694642904	22.640604637425785
86-87	22.60125206355024	27.419994565827288	27.300732743384387	22.67802062723808
88-89	22.758004336378907	27.23929100040623	27.217688896377723	22.785015766837144
90-91	22.755684510661027	27.267242785418393	27.251775699324128	22.725297004596452
92-93	22.81784223454073	27.221712531043686	27.379245045988437	22.581200188427147
94-95	22.585794294101362	27.360593723454063	27.38720877793336	22.666403204511216
96-97	22.645662346162172	27.4380676526926	27.433226291693185	22.483043709452044
98-99	23.85697353455277	29.340445152507854	27.595396920870535	19.207184392068843
100	22.72295432412431	30.388423263483144	21.468535818394333	25.420086593998214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3382.0
1	2808.5
2	3461.5
3	4943.0
4	6678.0
5	10864.0
6	14505.0
7	17505.5
8	19889.5
9	19833.0
10	17540.0
11	15209.5
12	16267.5
13	21425.0
14	33463.0
15	50098.5
16	63916.0
17	71720.5
18	72793.0
19	68072.5
20	60639.0
21	53615.5
22	49435.5
23	50562.5
24	57705.0
25	71158.0
26	89027.0
27	107847.0
28	128343.0
29	150674.5
30	172229.0
31	192432.5
32	212566.0
33	232889.0
34	254202.5
35	274592.0
36	295418.5
37	314631.5
38	330896.0
39	346586.5
40	361270.0
41	373848.0
42	381158.0
43	387866.0
44	395696.0
45	408544.0
46	421598.0
47	435794.0
48	447876.0
49	445306.5
50	438223.5
51	431205.0
52	409475.0
53	380391.0
54	357154.0
55	335329.5
56	312399.0
57	284167.0
58	254200.0
59	220877.5
60	185248.0
61	156694.5
62	128605.5
63	103392.0
64	84633.5
65	66085.0
66	52476.0
67	39186.5
68	25696.0
69	18498.5
70	13357.0
71	8626.5
72	5926.5
73	4283.5
74	3275.5
75	2403.0
76	1602.5
77	947.0
78	672.5
79	525.0
80	348.5
81	219.5
82	176.5
83	122.0
84	74.0
85	44.0
86	31.0
87	26.0
88	22.0
89	16.5
90	10.5
91	7.0
92	4.5
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0011981140561524233
5	0.0
6	2.1528611946488856E-4
7	0.0
8	0.0
9	0.0
10-11	0.004052995205578119
12-13	5.148146335029944E-5
14-15	0.0014321207077446934
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	220.0
20-21	829.0
22-23	1760.0
24-25	3541.0
26-27	5006.0
28-29	9035.0
30-31	18859.0
32-33	24398.0
34-35	34126.0
36-37	47194.0
38-39	55765.0
40-41	60279.0
42-43	74262.0
44-45	89517.0
46-47	87278.0
48-49	77248.0
50-51	67320.0
52-53	66209.0
54-55	71445.0
56-57	75853.0
58-59	75226.0
60-61	74577.0
62-63	74357.0
64-65	80496.0
66-67	85049.0
68-69	85872.0
70-71	84415.0
72-73	81001.0
74-75	84348.0
76-77	82008.0
78-79	88711.0
80-81	91527.0
82-83	89912.0
84-85	90778.0
86-87	97010.0
88-89	105244.0
90-91	112077.0
92-93	116223.0
94-95	134475.0
96-97	362302.0
98-99	660172.0
100-101	7057533.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.40824502048297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.75525427397278	73.14164741064816
2	7.553912768688302	12.450093886108407
3	1.9424447328817123	4.802203844581882
4	0.7373794656159812	2.430645907022183
5	0.35997756028087013	1.483255949475081
6	0.19506874536257018	0.9645163778206128
7	0.11183436522579357	0.6451251639866163
8	0.07282754626955378	0.4801272221777558
9	0.05398746610112262	0.40041111010466984
>10	0.21120400691926078	2.7810749099385657
>50	0.005112984755867195	0.2791112606032631
>100	9.737354298716281E-4	0.12570891333602008
>500	2.2348496388061945E-5	0.01607804419681065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.360266063690807E-6	0.0
4	0.0	1.216834588279805E-4	0.0	9.360266063690807E-6	0.0
5	0.0	1.310437248916713E-4	0.0	9.360266063690807E-6	0.0
6	1.0296292670059887E-4	1.404039909553621E-4	0.0	9.360266063690807E-6	0.0
7	1.0296292670059887E-4	1.5912452308274372E-4	0.0	9.360266063690807E-6	0.0
8	1.0296292670059887E-4	1.5912452308274372E-4	0.0	9.360266063690807E-6	0.0
9	1.0296292670059887E-4	2.9952851403810583E-4	0.0	2.808079819107242E-5	0.0
10-11	1.0296292670059887E-4	2.9952851403810583E-4	0.0	5.148146335029944E-5	0.0
12-13	1.0296292670059887E-4	2.9952851403810583E-4	0.0	6.552186244583565E-5	0.0
14-15	1.0296292670059887E-4	4.118517068023955E-4	0.0	7.020199547768106E-5	1.8720532127381615E-5
16-17	1.310437248916713E-4	4.305722389297771E-4	0.0	7.488212850952646E-5	1.8720532127381615E-5
18-19	1.357238579235167E-4	4.6333317015269496E-4	0.0	7.956226154137186E-5	3.2760931222917826E-5
20-21	1.404039909553621E-4	5.10134500471149E-4	0.0	9.828279366875347E-5	3.744106425476323E-5
22-23	1.404039909553621E-4	5.569358307896031E-4	0.0	1.1232319276428968E-4	4.212119728660863E-5
24-25	1.450841239872075E-4	5.850166289806754E-4	0.0	1.310437248916713E-4	4.6801330318454035E-5
26-27	1.4976425701905292E-4	5.990570280762117E-4	0.0	1.8720532127381614E-4	4.6801330318454035E-5
28-29	1.4976425701905292E-4	6.03737161108057E-4	0.0	5.10134500471149E-4	4.6801330318454035E-5
30-31	1.4976425701905292E-4	7.535014181271099E-4	0.0	0.0014227604416810027	4.6801330318454035E-5
32-33	1.4976425701905292E-4	7.581815511589554E-4	0.0	0.003673904429998642	5.148146335029944E-5
34-35	1.5444439005089832E-4	8.985855421143176E-4	0.0	0.006734711432825535	5.616159638214484E-5
36-37	1.5912452308274372E-4	0.001020269000942298	0.0	0.010375854931601258	5.616159638214484E-5
38-39	1.5912452308274372E-4	0.0010296292670059887	0.0	0.01467221705483534	5.616159638214484E-5
40-41	1.7784505521012533E-4	0.0011045113955155152	0.0	0.01905282157264264	6.552186244583565E-5
42-43	1.7784505521012533E-4	0.0011466325928021239	0.0	0.02365339234294667	7.488212850952646E-5
44-45	1.7784505521012533E-4	0.0012823564507256405	0.0	0.02874537708159447	8.424239457321726E-5
46-47	1.8720532127381614E-4	0.0013759591113625487	0.0	0.03330850678764374	8.424239457321726E-5
48-49	1.8720532127381614E-4	0.0013759591113625487	0.0	0.03863449817788381	8.424239457321726E-5
50-51	2.0124572036935235E-4	0.001553804166572674	0.0	0.043169547085742	9.360266063690807E-5
52-53	2.1528611946488856E-4	0.0015818849647637465	0.0	0.04763907413115436	9.828279366875347E-5
54-55	2.1996625249673396E-4	0.0015865650977955918	0.0	0.05201031838289797	1.0296292670059887E-4
56-57	2.2464638552857936E-4	0.0015912452308274373	0.0	0.056653010350488614	1.0296292670059887E-4
58-59	2.2932651856042476E-4	0.0015959253638592828	0.0	0.06171223415791349	1.0296292670059887E-4
60-61	2.527271837196518E-4	0.0016380465611458912	0.0	0.06668253543773331	1.0296292670059887E-4
62-63	2.6676758281518804E-4	0.0016848478914643452	0.0	0.07153115325872515	1.0296292670059887E-4
64-65	2.714477158470334E-4	0.0020077770706616784	0.0	0.07628148828604824	1.0296292670059887E-4
66-67	2.808079819107242E-4	0.002017137336725369	0.0	0.08121902863464514	1.0296292670059887E-4
68-69	2.90168247974415E-4	0.0020732989331075135	0.0	0.08642333656605722	1.0296292670059887E-4
70-71	2.9952851403810583E-4	0.002120100263425968	0.0	0.09136087691465412	1.1232319276428968E-4
72-73	3.3696957829286905E-4	0.0021294605294896584	0.0	0.09508158267497122	1.310437248916713E-4
74-75	3.4632984435655986E-4	0.0021481810616170403	0.0	0.0979458240904606	1.310437248916713E-4
76-77	3.6505037648394147E-4	0.002237103589222103	0.0	0.10083346617110922	1.357238579235167E-4
78-79	3.744106425476323E-4	0.0022651843874131753	0.0	0.10249491339741434	1.404039909553621E-4
80-81	3.837709086113231E-4	0.0022698645204450208	0.0	0.1030892902924587	1.404039909553621E-4
82-83	3.837709086113231E-4	0.002283904919540557	0.0	0.10359474465989801	1.404039909553621E-4
84-85	3.837709086113231E-4	0.0023166658507634747	0.0	0.1038615122427132	1.404039909553621E-4
86-87	3.837709086113231E-4	0.0023494267819863927	0.0	0.10393639437122273	1.404039909553621E-4
88	3.837709086113231E-4	0.0024991910390054456	0.0	0.10394575463728642	1.404039909553621E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15580	0.0	53.171402	1
GTATCAA	28685	0.0	37.69434	1
TCAACGC	34955	0.0	30.406204	4
ATCAACG	35380	0.0	30.052643	3
TATCAAC	36120	0.0	29.662378	2
CAACGCA	35895	0.0	29.61174	5
AACGCAG	36360	0.0	29.315557	6
ACGCAGA	41240	0.0	25.753063	7
CGCAGAG	43915	0.0	24.184477	8
GCAGAGT	48150	0.0	21.826015	9
GTACATG	34130	0.0	21.647846	1
GTGGTAT	7245	0.0	21.058397	1
TACATGG	34435	0.0	21.008066	2
TGGTATC	6825	0.0	20.596094	2
ACATGGG	34530	0.0	20.428993	3
GAGTACT	29715	0.0	20.328297	12-13
CAGAGTA	47280	0.0	19.671139	10-11
AGAGTAC	44165	0.0	18.986671	10-11
CATGGGG	25340	0.0	18.77311	4
GTACTTT	33265	0.0	18.159695	14-15
>>END_MODULE