FastQCFastQC Report
Fri 27 May 2016
SRR2139236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10985555
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211950.054.8440251
GTATCAA395550.038.8475271
TCAACGC518650.029.276644
ATCAACG519550.029.216973
TATCAAC524150.029.0656642
CAACGCA528950.028.7242725
AACGCAG539500.028.2254126
ACGCAGA605300.025.1498957
GTGGTAT83250.024.9430051
CGCAGAG644700.023.646418
TGGTATC83250.023.5685942
GCAGAGT684800.022.0517149
GTACATG444000.022.0192011
TACATGG440550.021.7202932
ACATGGG447400.020.9709723
GAGTACT437350.020.72186912-13
CAGAGTA677000.019.61041610-11
AGAGTAC646400.019.5847610-11
CATGGGG312200.019.1668434
AGTACTT485100.018.3370712-13
Produced by FastQC (version 0.11.5)