##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10985555
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54094262875203	33.0	33.0	33.0	33.0	33.0
2	32.55508101320325	33.0	33.0	33.0	33.0	33.0
3	32.544732969795334	33.0	33.0	33.0	33.0	33.0
4	36.45976065842827	37.0	37.0	37.0	37.0	37.0
5	36.50140561856001	37.0	37.0	37.0	37.0	37.0
6	36.502866263925675	37.0	37.0	37.0	37.0	37.0
7	36.4832027148378	37.0	37.0	37.0	37.0	37.0
8	36.47837437434886	37.0	37.0	37.0	37.0	37.0
9	36.493022246031266	37.0	37.0	37.0	37.0	37.0
10-11	36.44780746170767	37.0	37.0	37.0	37.0	37.0
12-13	36.42627910014561	37.0	37.0	37.0	37.0	37.0
14-15	38.35448723346249	40.0	37.0	40.0	37.0	40.0
16-17	38.33026688228314	40.0	37.0	40.0	37.0	40.0
18-19	38.38828188471133	40.0	37.0	40.0	37.0	40.0
20-21	38.39302741971332	40.0	37.0	40.0	37.0	40.0
22-23	38.39364696268494	40.0	37.0	40.0	37.0	40.0
24-25	38.36233582582438	40.0	37.0	40.0	37.0	40.0
26-27	38.235793195520756	40.0	37.0	40.0	37.0	40.0
28-29	38.17709805704246	40.0	37.0	40.0	37.0	40.0
30-31	38.07821543094083	40.0	37.0	40.0	33.0	40.0
32-33	37.989180951220035	40.0	37.0	40.0	33.0	40.0
34-35	37.963824172578406	40.0	37.0	40.0	33.0	40.0
36-37	37.89872384112473	40.0	37.0	40.0	33.0	40.0
38-39	37.84049853092147	40.0	37.0	40.0	33.0	40.0
40-41	37.807546820051456	40.0	37.0	40.0	33.0	40.0
42-43	37.74474898459229	40.0	37.0	40.0	33.0	40.0
44-45	37.66956966727929	40.0	37.0	40.0	33.0	40.0
46-47	37.61703430354663	40.0	37.0	40.0	33.0	40.0
48-49	37.588505572703724	40.0	37.0	40.0	33.0	40.0
50-51	37.50719747234269	40.0	37.0	40.0	33.0	40.0
52-53	37.406889727825586	40.0	37.0	40.0	33.0	40.0
54-55	37.23618240220411	40.0	37.0	40.0	33.0	40.0
56-57	37.12964458757771	37.0	37.0	40.0	33.0	40.0
58-59	37.00949613127884	37.0	37.0	40.0	33.0	40.0
60-61	36.88151645490326	37.0	37.0	40.0	33.0	40.0
62-63	36.73328735053232	37.0	37.0	40.0	33.0	40.0
64-65	36.549754683319485	37.0	37.0	40.0	33.0	40.0
66-67	36.34519698943315	37.0	37.0	40.0	33.0	40.0
68-69	36.141843007757984	37.0	37.0	40.0	33.0	40.0
70-71	35.89619528179952	37.0	35.0	37.0	33.0	40.0
72-73	35.71822875498363	37.0	33.0	37.0	33.0	40.0
74-75	35.538280038106834	37.0	33.0	37.0	33.0	40.0
76-77	33.6987578444454	35.0	33.0	37.0	27.0	37.0
78-79	35.011289309637846	37.0	33.0	37.0	33.0	37.0
80-81	35.072155343445814	37.0	33.0	37.0	33.0	37.0
82-83	34.937369860535625	37.0	33.0	37.0	33.0	37.0
84-85	34.79096041657432	37.0	33.0	37.0	33.0	37.0
86-87	34.698408098050905	37.0	33.0	37.0	33.0	37.0
88-89	34.58306237985754	37.0	33.0	37.0	33.0	37.0
90-91	34.5261912122252	37.0	33.0	37.0	33.0	37.0
92-93	34.457333666636664	37.0	33.0	37.0	33.0	37.0
94-95	34.47815264195483	37.0	33.0	37.0	33.0	37.0
96-97	34.468943787807895	37.0	33.0	37.0	33.0	37.0
98-99	34.46843589086468	37.0	33.0	37.0	33.0	37.0
100	34.44402867117293	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	3.0
17	2.0
18	5.0
19	24.0
20	122.0
21	470.0
22	1754.0
23	4802.0
24	11337.0
25	22192.0
26	38679.0
27	62564.0
28	92704.0
29	130052.0
30	176959.0
31	236378.0
32	311797.0
33	415914.0
34	564201.0
35	804076.0
36	1385929.0
37	3107232.0
38	3515152.0
39	103205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39155873326382	20.280459203017053	14.319631552525111	23.008350511194017
2	14.721604871123944	21.617041651514192	41.6518601017427	22.009493375619165
3	17.808331031067613	27.26839017236726	30.808129402656487	24.115149393908638
4	12.044411200402132	18.573461004292323	39.28321584586561	30.098911949439927
5	12.937179778354393	38.06407596156953	35.56129845055621	13.437445809519865
6	28.7019091731577	38.662938857540105	19.03248096748262	13.602671001819575
7	25.567046908417463	33.42440140712053	23.585626761688417	17.42292492277359
8	24.432265825440773	36.36419825853132	21.313315531167977	17.890220384859937
9	25.7703320405751	16.501642384021565	21.607784040041672	36.120241535361664
10-11	23.943560810125604	27.11151744068445	28.727279827824365	20.21764192136558
12-13	25.138485296938867	25.003608148065382	28.893671938666714	20.96423461632904
14-15	22.224471668919275	25.911372963294294	27.286928330215943	24.577227037570488
16-17	21.17507945661371	29.108975377211255	27.69749002212451	22.018455144050527
18-19	21.147966579749497	27.751001201122747	30.19591181328572	20.905120405842034
20-21	22.254052113347385	26.713247505810582	30.25817077118418	20.774529609657854
22-23	21.945199682739222	26.827153691579696	30.005688261424048	21.221958364257034
24-25	21.606760972690754	27.086914985656847	30.24540016693344	21.060923874718966
26-27	21.51058950931144	27.136885441224656	30.151184862772716	21.20134018669118
28-29	21.444092705250032	27.16011901147611	30.14662911135071	21.24915917192315
30-31	21.935717715204696	26.889897812792363	30.004152229677043	21.170232242325895
32-33	21.56412063874805	27.160605807368466	29.862875085266534	21.412398468616953
34-35	21.760952926909972	27.27695211193132	29.864949670664775	21.09714529049393
36-37	21.84495780353097	27.213077870874393	29.609831970222427	21.33213235537221
38-39	21.521204899888197	27.414241126233012	29.700490288735875	21.364063685142916
40-41	21.73952618098582	27.170020763173742	29.643932707874303	21.446520347966135
42-43	21.71051354747154	27.379797710268278	29.219337787511495	21.69035095474869
44-45	21.65566638921202	27.553237852230474	29.01096262762512	21.78013313093239
46-47	21.95072052575477	27.468134933976003	28.596995652879322	21.98414888738991
48-49	22.056076453167282	27.82128761359701	28.263702582091565	21.858933351144145
50-51	22.05609764644728	27.83225023313586	28.169377337442498	21.942274782974366
52-53	22.230790825051518	27.81000878953298	28.037673630052602	21.9215267553629
54-55	22.234776109958222	27.751656503105682	27.987861918629562	22.02570546830653
56-57	22.339079466567327	28.010559285542463	27.64782725841563	22.002533989474582
58-59	22.059151962313457	28.00535681006582	27.978000107497873	21.95749112012285
60-61	22.26362069871572	28.084689178061666	27.75967259555711	21.892017527665505
62-63	22.219860254322594	28.043425035458913	27.87635926267951	21.860355447538982
64-65	22.284828930516802	27.887130889402023	27.789400185412884	22.038639994668298
66-67	22.40693359927938	27.923225268791413	27.55722129512328	22.11261983680593
68-69	22.344495570780552	27.834135554853116	27.701566543376554	22.11980233098977
70-71	22.36195060510564	27.636124575981537	27.72456521956441	22.27735959934841
72-73	22.338051315413768	27.801472845749313	27.588475055060908	22.272000783776008
74-75	22.219932941432333	27.777129509876374	27.609675672382654	22.39326187630864
76-77	22.314176565274792	27.731889222414548	27.58012536428095	22.37380884802971
78-79	22.184138584754027	27.790568749299272	27.609033215263963	22.416259450682734
80-81	22.31893295895184	27.6664315575987	27.654956262765424	22.35967922068404
82-83	22.5065349616622	27.51028080746988	27.82033501593076	22.162849214937168
84-85	22.47044382171105	27.53213990820045	27.676691137255823	22.32072513283268
86-87	22.265485809297722	27.702482961516267	27.693416588409704	22.338614640776306
88-89	22.559193730125358	27.465804930713166	27.54048045526904	22.434520883892436
90-91	22.495048820035866	27.466439020724888	27.616498089002565	22.422014070236685
92-93	22.55656824270821	27.43745748552041	27.731873472308493	22.274100799462886
94-95	22.28431474813595	27.574965336650255	27.794471183415126	22.346248731798664
96-97	22.29124049854859	27.629196262517635	27.870610274837475	22.208952964096294
98-99	23.505861068958396	29.554432827997136	27.971623601540195	18.96808250150427
100	22.361347572468247	30.58136931963493	22.015019101394316	25.042264006502506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5438.0
1	4572.5
2	5807.0
3	8282.5
4	10820.0
5	17357.0
6	23300.0
7	28697.5
8	33452.5
9	33342.0
10	28711.5
11	24116.5
12	25342.0
13	33296.5
14	52284.5
15	78036.0
16	98379.0
17	109332.0
18	109846.5
19	101343.5
20	88811.5
21	76178.5
22	67477.5
23	66717.0
24	73989.0
25	88241.0
26	107817.0
27	128838.0
28	149465.0
29	172258.5
30	194657.5
31	215003.5
32	236906.0
33	258333.0
34	277128.0
35	294599.5
36	316195.0
37	337240.5
38	351168.5
39	361225.5
40	371055.0
41	380188.5
42	385943.0
43	390956.5
44	397476.0
45	411370.5
46	426643.0
47	441225.5
48	444397.0
49	434457.5
50	426003.5
51	418901.0
52	395367.0
53	360153.5
54	335712.5
55	315895.5
56	294349.5
57	265610.5
58	236079.5
59	207717.5
60	177383.5
61	153444.5
62	126556.5
63	102097.0
64	85389.5
65	66882.5
66	54063.5
67	40614.5
68	26138.5
69	18506.0
70	12921.0
71	7957.0
72	5410.0
73	4015.5
74	3265.5
75	2522.0
76	1616.5
77	853.5
78	636.0
79	551.5
80	375.5
81	227.0
82	175.0
83	124.0
84	83.5
85	57.0
86	41.0
87	29.5
88	21.0
89	17.5
90	14.0
91	11.0
92	8.0
93	6.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0011651664390192394
5	0.0
6	1.274400792677293E-4
7	0.0
8	0.0
9	0.0
10-11	0.004487711362785039
12-13	2.2757157012094518E-5
14-15	0.0012516436356651986
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	872.0
22-23	1981.0
24-25	4956.0
26-27	6600.0
28-29	11604.0
30-31	21780.0
32-33	27662.0
34-35	39307.0
36-37	52137.0
38-39	62756.0
40-41	69556.0
42-43	88795.0
44-45	108092.0
46-47	104804.0
48-49	91648.0
50-51	77607.0
52-53	74027.0
54-55	79604.0
56-57	86505.0
58-59	86459.0
60-61	87337.0
62-63	85910.0
64-65	91470.0
66-67	97680.0
68-69	98241.0
70-71	96285.0
72-73	91485.0
74-75	95574.0
76-77	93241.0
78-79	101475.0
80-81	105100.0
82-83	101449.0
84-85	102661.0
86-87	108441.0
88-89	116530.0
90-91	123937.0
92-93	126683.0
94-95	144802.0
96-97	352961.0
98-99	644741.0
100-101	7022524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.82679234763691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.65144691354064	70.84585298650669
2	8.085221714767785	13.070050732482812
3	2.195369331725952	5.3233398330535175
4	0.8858038806650586	2.8638674529298256
5	0.4382128309193718	1.7709668744395095
6	0.24055794744594505	1.1666116359472292
7	0.14369425163658897	0.8130041807005762
8	0.08697150384301319	0.5623702145024746
9	0.060039680549532015	0.4367533313155931
>10	0.2060030980958001	2.632166899734682
>50	0.005438034244961868	0.30050168312126746
>100	0.0011735726893678695	0.15056511111093449
>500	2.2413599975913512E-5	0.01056174104962422
>1k	4.482627605500593E-5	0.05338732310516162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.102862804837808E-6	9.102862804837808E-6
2	0.0	1.8205725609675616E-5	0.0	9.102862804837808E-6	9.102862804837808E-6
3	0.0	1.8205725609675616E-5	0.0	1.8205725609675616E-5	9.102862804837808E-6
4	0.0	5.461717682902685E-5	0.0	1.8205725609675616E-5	9.102862804837808E-6
5	0.0	7.282290243870246E-5	0.0	1.8205725609675616E-5	9.102862804837808E-6
6	0.0	7.282290243870246E-5	0.0	1.8205725609675616E-5	9.102862804837808E-6
7	0.0	9.102862804837807E-5	0.0	5.461717682902685E-5	9.102862804837808E-6
8	0.0	1.092343536580537E-4	0.0	5.461717682902685E-5	9.102862804837808E-6
9	0.0	1.7295439329191833E-4	0.0	6.372003963386465E-5	9.102862804837808E-6
10-11	0.0	1.7295439329191833E-4	0.0	6.372003963386465E-5	9.102862804837808E-6
12-13	0.0	1.8205725609675614E-4	0.0	7.282290243870246E-5	1.3654294207256712E-5
14-15	0.0	2.503287271330397E-4	0.0	1.1378578506047259E-4	2.275715701209452E-5
16-17	0.0	2.548801585354586E-4	0.0	1.4109437347498602E-4	2.7308588414513423E-5
18-19	0.0	2.8218874694997204E-4	0.0	1.6385153048708055E-4	3.1860019816932326E-5
20-21	0.0	3.0949733536448547E-4	0.0	2.0481441310885067E-4	4.096288262177014E-5
22-23	0.0	3.3225449237657997E-4	0.0	2.3667443292578298E-4	5.006574542660794E-5
24-25	0.0	3.550116493886745E-4	0.0	2.9584304115722873E-4	6.827147103628356E-5
26-27	0.0	3.641145121935123E-4	0.0	4.4148884603463365E-4	7.282290243870246E-5
28-29	0.0	3.641145121935123E-4	0.0	0.0010058663399345777	7.282290243870246E-5
30-31	0.0	4.3238598322979584E-4	0.0	0.002080004150905439	7.737433384112137E-5
32-33	0.0	4.369374146322148E-4	0.0	0.003827753809434298	9.102862804837807E-5
34-35	0.0	5.507231996926873E-4	0.0	0.006540406925275965	9.102862804837807E-5
36-37	0.0	6.645089847531599E-4	0.0	0.009944877614285304	9.558005945079698E-5
38-39	0.0	6.781632789604167E-4	0.0	0.01439617752585099	1.0013149085321588E-4
40-41	0.0	7.737433384112137E-4	0.0	0.019416406362719046	1.0013149085321588E-4
42-43	0.0	7.965004954233082E-4	0.0	0.023908669156906502	1.0013149085321588E-4
44-45	0.0	9.148377118861997E-4	0.0	0.0288924865425552	1.0013149085321588E-4
46-47	0.0	0.001001314908532159	0.0	0.03396733255625228	1.0013149085321588E-4
48-49	0.0	0.0010058663399345777	0.0	0.03928340443427756	1.092343536580537E-4
50-51	0.0	0.0010695863795684422	0.0	0.04419895034888997	1.1833721646289149E-4
52-53	0.0	0.0010786892423732802	0.0	0.04915090771472175	1.228886478653104E-4
54-55	0.0	0.0010968949679829557	0.0	0.05409376221774867	1.319915106701482E-4
56-57	0.0	0.0011287549877998882	0.0	0.05842672491285147	1.365429420725671E-4
58-59	0.0	0.0011287549877998882	0.0	0.06309194210033084	1.365429420725671E-4
60-61	0.0	0.0011333064192023071	0.0	0.06784818791585859	1.365429420725671E-4
62-63	0.0	0.0011469607134095637	0.0	0.07272277094784924	1.365429420725671E-4
64-65	0.0	0.0012835036554821309	0.0	0.07690553640667222	1.365429420725671E-4
66-67	0.0	0.0012880550868845497	0.0	0.08192121381213785	1.365429420725671E-4
68-69	0.0	0.0013244665381039011	0.0	0.0872554914157728	1.365429420725671E-4
70-71	0.0	0.0013517751265184144	0.0	0.09168403417032639	1.6385153048708055E-4
72-73	0.0	0.0013745322835305089	0.0	0.09570294809866228	1.8205725609675614E-4
74-75	0.0	0.0014245980289571169	0.0	0.09877971572669747	1.8205725609675614E-4
76-77	0.0	0.0014928694999934004	0.0	0.1019884748654028	1.8205725609675614E-4
78-79	0.0	0.0015201780884079139	0.0	0.10402296470228405	1.8205725609675614E-4
80-81	0.0	0.0015201780884079139	0.0	0.10469202511843961	1.8660868749917505E-4
82-83	0.0	0.0015292809512127516	0.0	0.10519723400410813	1.9116011890159395E-4
84-85	0.0	0.0015383838140175894	0.0	0.10539294555441214	1.9116011890159395E-4
86-87	0.0	0.0015747952652369406	0.0	0.10549762847666777	1.9116011890159395E-4
88	0.0	0.0016749267560901566	0.0	0.10551128277087503	1.9116011890159395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21195	0.0	54.844025	1
GTATCAA	39555	0.0	38.847527	1
TCAACGC	51865	0.0	29.27664	4
ATCAACG	51955	0.0	29.21697	3
TATCAAC	52415	0.0	29.065664	2
CAACGCA	52895	0.0	28.724272	5
AACGCAG	53950	0.0	28.225412	6
ACGCAGA	60530	0.0	25.149895	7
GTGGTAT	8325	0.0	24.943005	1
CGCAGAG	64470	0.0	23.64641	8
TGGTATC	8325	0.0	23.568594	2
GCAGAGT	68480	0.0	22.051714	9
GTACATG	44400	0.0	22.019201	1
TACATGG	44055	0.0	21.720293	2
ACATGGG	44740	0.0	20.970972	3
GAGTACT	43735	0.0	20.721869	12-13
CAGAGTA	67700	0.0	19.610416	10-11
AGAGTAC	64640	0.0	19.58476	10-11
CATGGGG	31220	0.0	19.166843	4
AGTACTT	48510	0.0	18.33707	12-13
>>END_MODULE