FastQCFastQC Report
Fri 27 May 2016
SRR2139235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10715245
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148700.044.1007541
CTTATAC85600.036.6349751
GTATCAA261500.034.9164431
TATACAC124800.028.7720663
TCAACGC309300.028.7221414
ATCAACG311750.028.4686323
CAACGCA316700.028.0533815
TATCAAC323850.027.6724572
AACGCAG323850.027.4607666
TTATACA131800.024.7134592
ACGCAGA369350.024.0895067
GTACATG317350.022.9571821
CGCAGAG395450.022.4893638
TACATGG323100.022.0526122
TACACAT170250.021.3981635
ACATGGG323100.021.3421023
ACACATC153600.021.2077316
ACATCTC156750.021.0032168
GCAGAGT438850.019.9991349
GAGTACT247350.019.61351612-13
Produced by FastQC (version 0.11.5)