##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10715245
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52659038594078	33.0	33.0	33.0	33.0	33.0
2	32.54567357069297	33.0	33.0	33.0	33.0	33.0
3	32.5412465137288	33.0	33.0	33.0	33.0	33.0
4	36.445762929359056	37.0	37.0	37.0	37.0	37.0
5	36.48110985796405	37.0	37.0	37.0	37.0	37.0
6	36.480332460900335	37.0	37.0	37.0	37.0	37.0
7	36.45970680091776	37.0	37.0	37.0	37.0	37.0
8	36.452239869457024	37.0	37.0	37.0	37.0	37.0
9	36.47803638647553	37.0	37.0	37.0	37.0	37.0
10-11	36.42814611331799	37.0	37.0	37.0	37.0	37.0
12-13	36.409255877957065	37.0	37.0	37.0	37.0	37.0
14-15	38.354765430001834	40.0	37.0	40.0	37.0	40.0
16-17	38.31939419024017	40.0	37.0	40.0	37.0	40.0
18-19	38.34447000511888	40.0	37.0	40.0	37.0	40.0
20-21	38.339381843407295	40.0	37.0	40.0	37.0	40.0
22-23	38.33747639238344	40.0	37.0	40.0	37.0	40.0
24-25	38.31151045044763	40.0	37.0	40.0	37.0	40.0
26-27	38.18649973850379	40.0	37.0	40.0	37.0	40.0
28-29	38.127596944798086	40.0	37.0	40.0	33.0	40.0
30-31	38.05588169378978	40.0	37.0	40.0	33.0	40.0
32-33	37.9791069761352	40.0	37.0	40.0	33.0	40.0
34-35	37.957158711903475	40.0	37.0	40.0	33.0	40.0
36-37	37.89361638779418	40.0	37.0	40.0	33.0	40.0
38-39	37.831922982676524	40.0	37.0	40.0	33.0	40.0
40-41	37.80896381898627	40.0	37.0	40.0	33.0	40.0
42-43	37.75589929965251	40.0	37.0	40.0	33.0	40.0
44-45	37.684042304589724	40.0	37.0	40.0	33.0	40.0
46-47	37.637904258948396	40.0	37.0	40.0	33.0	40.0
48-49	37.615204059543714	40.0	37.0	40.0	33.0	40.0
50-51	37.5187421811235	40.0	37.0	40.0	33.0	40.0
52-53	37.411498643287985	40.0	37.0	40.0	33.0	40.0
54-55	37.22950637645609	40.0	37.0	40.0	33.0	40.0
56-57	37.109175619349486	37.0	37.0	40.0	33.0	40.0
58-59	36.98394581968524	37.0	37.0	40.0	33.0	40.0
60-61	36.83771592020922	37.0	37.0	40.0	33.0	40.0
62-63	36.67446541731072	37.0	37.0	40.0	33.0	40.0
64-65	36.483673420042926	37.0	37.0	40.0	33.0	40.0
66-67	36.27242913922197	37.0	37.0	40.0	33.0	40.0
68-69	36.057529806664775	37.0	37.0	40.0	33.0	40.0
70-71	35.79758371781344	37.0	33.0	37.0	33.0	40.0
72-73	35.60490469244266	37.0	33.0	37.0	33.0	40.0
74-75	35.41392244072922	37.0	33.0	37.0	33.0	40.0
76-77	33.59050197500634	35.0	33.0	37.0	27.0	37.0
78-79	34.90363655457122	37.0	33.0	37.0	33.0	37.0
80-81	34.9664966918142	37.0	33.0	37.0	33.0	37.0
82-83	34.838713269405496	37.0	33.0	37.0	33.0	37.0
84-85	34.693454884641255	37.0	33.0	37.0	33.0	37.0
86-87	34.59752966239495	37.0	33.0	37.0	33.0	37.0
88-89	34.48056163797784	37.0	33.0	37.0	30.0	37.0
90-91	34.419179841561714	37.0	33.0	37.0	30.0	37.0
92-93	34.352292155962616	37.0	33.0	37.0	27.0	37.0
94-95	34.37402736925745	37.0	33.0	37.0	33.0	37.0
96-97	34.36134252047922	37.0	33.0	37.0	33.0	37.0
98-99	34.36163555104975	37.0	33.0	37.0	33.0	37.0
100	34.3414943985445	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	15.0
20	103.0
21	460.0
22	1791.0
23	5271.0
24	12221.0
25	24124.0
26	41443.0
27	65560.0
28	96320.0
29	134159.0
30	180533.0
31	237815.0
32	312480.0
33	414701.0
34	563523.0
35	805855.0
36	1376606.0
37	3073049.0
38	3297946.0
39	71264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64458208841702	20.048034365989764	14.042889359972637	24.26449418562058
2	14.531874912799475	20.991773869846185	41.70967626031883	22.766674957035512
3	18.04924665744927	26.265344376166855	30.483894675296742	25.20151429108714
4	12.35838000104152	18.2144611006617	38.46815312719841	30.95900577109837
5	13.404303867993686	37.69786878414819	35.538048826695054	13.35977852116307
6	30.006900457218283	37.71347181283616	18.72602478415773	13.55360294578783
7	26.40745965211248	32.52432398885887	23.120115312342367	17.948101046686286
8	24.44749513426898	36.80319022103554	21.021469877730283	17.727844766965198
9	25.499818249606054	16.682810332381575	20.982693349522105	36.83467806849027
10-11	24.25207740462455	26.604983829860945	28.737107099965915	20.405831665548586
12-13	25.163066779079745	24.152688292060972	28.766181855715438	21.918063073143845
14-15	22.262819636391804	25.445882238204852	26.329126032829357	25.962172092573987
16-17	21.948420218109803	28.71100474137549	26.8964498711882	22.444125169326508
18-19	21.993575508539468	27.772594093742143	28.50214810767276	21.731682290045633
20-21	22.794553304897136	26.84606977531074	28.506426749493656	21.852950170298467
22-23	22.444715329155258	27.0574064541601	28.312061053597372	22.185817163087272
24-25	22.396530121165785	27.155192553657425	28.456826552315995	21.991450772860794
26-27	22.300057881016873	27.256159640289745	28.459184544442408	21.984597934250974
28-29	22.039293296969202	27.142354856399265	28.55185902117891	22.266492825452627
30-31	22.48949924127608	26.86737064387273	28.61217144780349	22.030958667047702
32-33	22.256516915653105	27.231056748033584	28.384416535444064	22.12800980086925
34-35	22.31328658170046	27.265658582626656	28.52371982146918	21.897335014203705
36-37	22.571073138546417	27.112112003457646	28.191824988546184	22.12498986944976
38-39	22.280450780876794	27.167124270219983	28.35118114868186	22.201243800221363
40-41	22.39182742732411	26.991907086455626	28.378373177707093	22.237892308513175
42-43	22.381920191610817	27.105671312464853	28.17248316778722	22.33992532813711
44-45	22.259787368030263	27.247324700997407	28.040010693409545	22.45287723756278
46-47	22.573863410516708	27.11058667529236	27.67385650163642	22.641693412554513
48-49	22.563949855440736	27.315048218678772	27.575051591395077	22.54595033448541
50-51	22.475330020303392	27.419178593563004	27.640372188153712	22.465119197979895
52-53	22.675665772829902	27.428466572130418	27.607794917271566	22.288072737768115
54-55	22.571251887972814	27.414264012608964	27.38556394588379	22.62892015353443
56-57	22.713189699022422	27.656609011185125	27.146293691914387	22.48390759787807
58-59	22.49896369272923	27.688160913461417	27.475278468229565	22.337596925579792
60-61	22.706459077622604	27.81264209158818	27.155047296200063	22.32585153458915
62-63	22.51424753977258	27.94007928078446	27.25205629612751	22.293616883315455
64-65	22.679808385522122	27.67961435089387	27.182813551584584	22.457763711999426
66-67	22.648152128810477	27.651756899581148	27.084052277907457	22.616038693700922
68-69	22.633161394089065	27.55327262678467	27.195117682775134	22.618448296351133
70-71	22.660240524615844	27.43122380123436	27.18705138466988	22.721484289479918
72-73	22.690282676317914	27.45837894868806	27.12809132159429	22.723247053399735
74-75	22.592223491439327	27.5919881269047	27.085160893373008	22.730627488282977
76-77	22.719424034432244	27.55183622035401	27.001146783414615	22.727592961799132
78-79	22.5997280015465	27.554336387757072	27.079311100613563	22.766624510082867
80-81	22.5878826718081	27.545175194138377	27.156261321123015	22.710680812930505
82-83	22.740650453936823	27.429497939929824	27.202727604607922	22.62712400152543
84-85	22.71077327222424	27.469412849677223	27.181656093675766	22.63815778442277
86-87	22.58842204340132	27.558323850330694	27.174160720425437	22.679093385842545
88-89	22.70598467211354	27.470717603476846	27.088184018937405	22.73511370547221
90-91	22.686959811840644	27.5685221282847	27.098970166432494	22.64554789344216
92-93	22.745728156312648	27.49301085429417	27.18330262389203	22.577958365501154
94-95	22.485974718351102	27.623687273467546	27.229554362625997	22.660783645555355
96-97	22.608886302244365	27.734338060772878	27.2372165644884	22.419559072494362
98-99	23.786059599014035	29.619891051222474	27.42892807315458	19.16512127660891
100	22.586080368068345	30.73258476170403	21.31694131098685	25.364393559240778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2509.0
1	2177.0
2	2961.0
3	4266.5
4	5643.0
5	9217.5
6	12297.0
7	14358.5
8	15752.0
9	15407.5
10	13754.5
11	12133.0
12	12908.5
13	16781.5
14	26218.0
15	39583.5
16	50664.0
17	56921.5
18	57950.0
19	54496.5
20	49179.0
21	44340.5
22	42299.0
23	44710.5
24	51290.0
25	62874.0
26	78158.0
27	95483.5
28	115818.0
29	138453.5
30	160365.0
31	182371.0
32	205361.0
33	227082.5
34	247824.0
35	266887.5
36	288120.0
37	309560.5
38	327307.5
39	342293.5
40	356378.0
41	371094.5
42	381921.0
43	391403.5
44	400132.5
45	413540.5
46	436571.0
47	461655.5
48	470345.5
49	463862.0
50	459395.5
51	453700.5
52	431206.0
53	397314.0
54	369321.0
55	344900.0
56	318133.5
57	287363.0
58	256533.0
59	222071.0
60	184153.5
61	153169.5
62	124832.5
63	98920.0
64	77362.0
65	56371.5
66	41885.0
67	31357.0
68	21163.5
69	14351.5
70	10271.5
71	7423.0
72	5323.0
73	3735.5
74	2712.5
75	1978.0
76	1277.0
77	720.0
78	513.0
79	398.5
80	267.5
81	167.5
82	128.0
83	83.5
84	53.5
85	41.0
86	31.5
87	22.5
88	14.0
89	9.5
90	9.0
91	9.0
92	7.0
93	4.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0011852272159899283
5	0.0
6	1.2132247092810291E-4
7	0.0
8	0.0
9	0.0
10-11	0.0040409715316821965
12-13	2.799749329110067E-5
14-15	0.0013065496869180313
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	213.0
20-21	810.0
22-23	1672.0
24-25	3358.0
26-27	4906.0
28-29	8264.0
30-31	17673.0
32-33	21661.0
34-35	29478.0
36-37	41442.0
38-39	49667.0
40-41	52231.0
42-43	63107.0
44-45	74960.0
46-47	73018.0
48-49	67197.0
50-51	60166.0
52-53	59407.0
54-55	68491.0
56-57	72595.0
58-59	73356.0
60-61	75713.0
62-63	74924.0
64-65	78363.0
66-67	81655.0
68-69	73722.0
70-71	73283.0
72-73	70221.0
74-75	72023.0
76-77	70655.0
78-79	77619.0
80-81	79568.0
82-83	78486.0
84-85	79459.0
86-87	84954.0
88-89	92847.0
90-91	99512.0
92-93	105103.0
94-95	130930.0
96-97	367642.0
98-99	679298.0
100-101	7325596.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.78497330360777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95565012821272	71.93450497637343
2	8.131180467574675	13.300167549348235
3	2.1204703532471796	5.202678336942368
4	0.7757456808727711	2.5377735920227447
5	0.37945067402418586	1.5516681622552007
6	0.20031542532725718	0.9829675027614337
7	0.11339816014400057	0.6491985850038727
8	0.08175805799766361	0.5349264472554986
9	0.053091308547304004	0.3907864126975375
>10	0.1817647967982562	2.3940383245021857
>50	0.005406334746172473	0.2967155452811844
>100	0.0017460212919861696	0.21324864277230066
>500	2.2591215879646886E-5	0.011325922784067447
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8664995527400448E-5
2	0.0	2.799749329110067E-5	0.0	9.332497763700224E-6	1.8664995527400448E-5
3	0.0	2.799749329110067E-5	0.0	9.332497763700224E-6	1.8664995527400448E-5
4	0.0	9.332497763700224E-5	0.0	9.332497763700224E-6	1.8664995527400448E-5
5	0.0	1.0265747540070246E-4	0.0	9.332497763700224E-6	1.8664995527400448E-5
6	0.0	1.0265747540070246E-4	0.0	9.332497763700224E-6	1.8664995527400448E-5
7	0.0	1.1198997316440267E-4	0.0	9.332497763700224E-6	1.8664995527400448E-5
8	0.0	1.1198997316440267E-4	0.0	9.332497763700224E-6	5.599498658220134E-5
9	1.8664995527400448E-5	2.5197743961990603E-4	0.0	5.599498658220134E-5	5.599498658220134E-5
10-11	1.8664995527400448E-5	2.5197743961990603E-4	0.0	7.465998210960179E-5	5.599498658220134E-5
12-13	1.8664995527400448E-5	2.5197743961990603E-4	0.0	1.1198997316440267E-4	7.465998210960179E-5
14-15	2.7997493291100672E-5	3.872986571935593E-4	0.0	1.1198997316440267E-4	1.0732372428255257E-4
16-17	4.666248881850112E-5	4.012974038391096E-4	0.0	1.1198997316440267E-4	1.3065496869180314E-4
18-19	4.666248881850112E-5	4.432936437757606E-4	0.0	1.1665622204625278E-4	1.633187108647539E-4
20-21	4.666248881850112E-5	5.039548792398121E-4	0.0	1.3532121757365325E-4	1.7731745751030424E-4
22-23	5.599498658220134E-5	6.112786035223646E-4	0.0	1.4931996421920358E-4	1.959824530377047E-4
24-25	6.532748434590157E-5	6.952710833956666E-4	0.0	1.7731745751030424E-4	2.1464744856510514E-4
26-27	6.999373322775167E-5	7.279348255686174E-4	0.0	2.333124440925056E-4	2.1464744856510514E-4
28-29	7.932623099145191E-5	7.279348255686174E-4	0.0	4.246286482483602E-4	2.379786929743557E-4
30-31	9.332497763700224E-5	8.492572964967204E-4	0.0	8.305923009693198E-4	2.426449418562058E-4
32-33	9.332497763700224E-5	8.77254789787821E-4	0.0	0.001997154521431848	2.5197743961990603E-4
34-35	1.0265747540070245E-4	0.001049905998416275	0.0	0.003644340376724937	2.799749329110067E-4
36-37	1.1665622204625278E-4	0.0012318897048084294	0.0	0.005856142346721891	2.799749329110067E-4
38-39	1.213224709281029E-4	0.0012645534469813802	0.0	0.009089852821844017	3.0330617732025726E-4
40-41	1.2598871980995302E-4	0.0013998746645550336	0.0	0.01211358209728289	3.0797242620210735E-4
42-43	1.3065496869180314E-4	0.0014325384067279843	0.0	0.015020655150675511	3.1263867508395744E-4
44-45	1.3065496869180314E-4	0.0015538608776560874	0.0	0.018515675563181243	3.173049239658076E-4
46-47	1.586524619829038E-4	0.0016518521041749396	0.0	0.022323334650770934	3.2197117284765773E-4
48-49	1.586524619829038E-4	0.0016518521041749396	0.0	0.02592101253867737	3.4530241725690825E-4
50-51	1.586524619829038E-4	0.0018291695616852437	0.0	0.02946269544000161	3.499686661387584E-4
52-53	1.586524619829038E-4	0.001838502059448944	0.0	0.03312103456337209	3.8263240831170914E-4
54-55	1.633187108647539E-4	0.0018664995527400447	0.0	0.036606722478114126	3.919649060754094E-4
56-57	1.8664995527400448E-4	0.0019038295437948456	0.0	0.04045171155675862	3.919649060754094E-4
58-59	2.0531495080140493E-4	0.0019038295437948456	0.0	0.04441335685744936	3.966311549572595E-4
60-61	2.0531495080140493E-4	0.0019458257837314964	0.0	0.048118358469638356	4.059636527209597E-4
62-63	2.1464744856510514E-4	0.0019878220236681475	0.0	0.052411307440940455	4.1996239936651004E-4
64-65	2.1931369744695523E-4	0.002333124440925056	0.0	0.056503607710323006	4.246286482483602E-4
66-67	2.2864619521065547E-4	0.0023937856763891073	0.0	0.060679900459578856	4.386273948939105E-4
68-69	2.426449418562058E-4	0.00250110940067166	0.0	0.06509417190180905	4.386273948939105E-4
70-71	2.426449418562058E-4	0.0025431056406083107	0.0	0.06893916098045355	4.61958639303161E-4
72-73	2.426449418562058E-4	0.0025617706361357115	0.0	0.07232219141979487	5.319523725309127E-4
74-75	2.426449418562058E-4	0.0026224318715997626	0.0	0.0749772870335676	5.319523725309127E-4
76-77	2.5197743961990603E-4	0.0027530868402915656	0.0	0.07760438515404922	5.319523725309127E-4
78-79	2.5197743961990603E-4	0.002809081826873767	0.0	0.07934956223586115	5.412848702946129E-4
80-81	2.566436885017561E-4	0.002809081826873767	0.0	0.07986284961286466	5.506173680583132E-4
82-83	2.6130993738360627E-4	0.0028137480757556175	0.0	0.08033414074993152	5.786148613494139E-4
84-85	2.6130993738360627E-4	0.0028184143246374675	0.0	0.08053478945185108	5.786148613494139E-4
86-87	2.7064243514730645E-4	0.0029210718000381697	0.0	0.08063744692725178	5.879473591131142E-4
88	2.7064243514730645E-4	0.0030983892575484743	0.0	0.08065144567389733	6.159448524042147E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14870	0.0	44.100754	1
CTTATAC	8560	0.0	36.634975	1
GTATCAA	26150	0.0	34.916443	1
TATACAC	12480	0.0	28.772066	3
TCAACGC	30930	0.0	28.722141	4
ATCAACG	31175	0.0	28.468632	3
CAACGCA	31670	0.0	28.053381	5
TATCAAC	32385	0.0	27.672457	2
AACGCAG	32385	0.0	27.460766	6
TTATACA	13180	0.0	24.713459	2
ACGCAGA	36935	0.0	24.089506	7
GTACATG	31735	0.0	22.957182	1
CGCAGAG	39545	0.0	22.489363	8
TACATGG	32310	0.0	22.052612	2
TACACAT	17025	0.0	21.398163	5
ACATGGG	32310	0.0	21.342102	3
ACACATC	15360	0.0	21.207731	6
ACATCTC	15675	0.0	21.003216	8
GCAGAGT	43885	0.0	19.999134	9
GAGTACT	24735	0.0	19.613516	12-13
>>END_MODULE