FastQCFastQC Report
Fri 27 May 2016
SRR2139234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9219827
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC125350.051.9513631
GGTATCA142900.049.302021
TATACAC165250.041.5584873
TTATACA173200.038.9668662
GTATCAA258100.036.3791051
ATCAACG322450.028.6860313
TCAACGC324250.028.553354
TACACAT244650.028.5051255
TATCAAC328200.028.2865982
CAACGCA328950.028.1338945
AACGCAG336950.027.4910476
ACACATC254500.026.1880846
ATACACA281950.025.4682754
ACATCTC267850.024.8357098
ACGCAGA384200.024.1540437
CACATCT275100.024.1192677
CGCAGAG385550.024.0369328
CATCTCC309850.021.2098679
GCAGAGT444600.020.7588969
GTACATG318650.020.3303071
Produced by FastQC (version 0.11.5)